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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHFF1

S. pombe expression vector with nmt1 promoter

Catalog number RDB06580
Resource name pHFF1
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP1 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06580 pHFF1 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06580z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14494_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14494_A6L7a.seq check
            >06580_14494_A6L7_nmt1Fw_A07_01_ABI24.ab1
                1 TGCATTTATT AATCAGGAAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
               61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
              121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATATA
              181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC
              241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC
              301 CTATGCATCA CCACCATCAT CATATGGATT ATAAGGATGA TGACGATAAA CCCATGGACT
              361 ACAAGGACGA CGATGACAAA GATATCATGA GATCTCGAGC CCGGGCGGCC GCTAACTAAC
              421 TAAGGGTAAA AGGAATGTCT CCCTTGCCAG TACTGCTAGG GTTTTTCTTT CAAACTATGG
              481 AAGCCCATTC AAGCTGCATA TTACGATTTT GTTTTTCGCT TTTAGAAAGT GGTTTAGATG
              541 AGATAATAGA AAAATTCTTG ATCTCCGACA ACGAGTACTT TTATTTTTTT TGCTAATCAC
              601 TTTACTCAAT ATTAGCTCGA AATCGTAGAA ACGTAGACGG GTGCGGGATA CCGAGTGGTG
              661 TAGTTAAGAA TTTTTATAAA CCACGTGGCC CAAAAATATG AACCCAAAAC GTTTATACAT
              721 GAGTATACTT TAAGAAGGCT ATACCCCTTC GTGTTAGATG TAGTTTTAGC TACCCAACCC
              781 GAGTCTATGA GCTTGACTTC AGATGTAGAA GGCATTAAAT CGTTTTGAAT ATTAATTAAA
              841 AAACGATGAA AATTAAATAT TTAAAAGCAA TCATACGCTG AAAATTTAGT GCTGTGGCTA
              901 ATCCTTCAAC ATGGAAATGC CATAAAAGTG ACTTTGACAA AAAAAAAAAG TATATACAGG
              961 TAGTAAACTC ATCTACTTTC ATTTGACTTT GTTTTACAGC ATGTGGAGGA GGATATTTTA
             1021 TTGCTAATCG TAGTTACATT CAATAGGTTA TACTATGAAA TTTCGACAGA TTGGCGCATG
            1081 CATGCAAGAG CATTGTGCTT TTGGGGA
            //
            Primer: f1ori_R3
            Sequence file: RDB14494_A6L7b.seq check
            >06580_14494_A6L7_f1ori_R3_C07_07_ABI24.ab1
                1 GCGATGGTGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC
              241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG
              301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC
              361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT
              421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA
              481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG
              541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT
              601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT
              661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG
              721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC
              781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG
              841 TTTTGATAAA TTGTAAACTC TTACTCAAAA AAAGAATGTT AAAACTAATT TAACCTTTTT
              901 TATAAAGGCT CATGGCGTTA AAGAGGATAG TAAAAATTCT ACAATTTTTT TAAATTTAAT
              961 GTCACAATTG TCTTGCGAAT ATAATTCTAT TAAATTTTCG CTAACTGCTG TTTTAGTACT
             1021 ATTTACCTTT CATATGCCCT TATATGCAAA CTTTAGTCAT GGAGGAATGC AATTATTTTT
            1081 GCATCGTTCA TGTACTGAGC GCTTTTTTAT CATTTTAAAG GTA
            //
            Primer: LEU2pro_F
            Sequence file: RDB14494_A6L7c.seq check
            >06580_14494_A6L7_LEU2pro_F_B07_04_ABI24.ab1
                1 AACCATTGCA GTTGAAATAT TATTTAAGGA CCTATTGTTT TTTCCAATAG GTGGTTAGCA
               61 ATCGTCTTAC TTTCTAACTT TTCTTACCTT TTACATTTCA GCAATATATA TATATATTTC
              121 AAGGATATAC CATTCTAATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG
              181 TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT
              241 CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG
              301 GTGTTCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGCTGATGCC GTTTTGTTAG
              361 GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA
              421 AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT
              481 CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG
              541 TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG
              601 TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG
              661 CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAGCTAATG
              721 TTTTGGCCTC TTCAAGATTA TGGAGAAAAA CTGTGGAGGA AACCATCAAG AACGAATTCC
              781 CTACATTGAA AGGTTCAACA TCAATTGATT GATTCTGCCG CCATGATCCT AGTTAAGAAC
              841 CCAACCCACC TAAATGGTAT TATAATCACC AGCAACATGT TTGGTGACAT CATCTCCGAT
              901 GAAGCCTCCG TTATCCCAGG TTTCCTTGGG TTTGTTGCCA TCTGCGTCCC TTGGGCCTCT
              961 TTGCCAGACA GACACGCAAT TTGGGTTTGT ACGAACCATG CCACGGGTCT GCTCCAGATT
             1021 TGCCAAGGAT TAGTTCACCT ATCGCCACTA TCTTGTCTGC CTGCCAATGA ATGTGAATGT
            1081 CATGGACTTG CCTGAGAGTT AGCCATTGGT AG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            user report Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270.

            2022.02.09

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