Resource data sheet
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pHIS3H

S. pombe vector for chromosomal integration

Catalog number RDB06562
Resource name pHIS3H
Clone info. S. pombe vector for chromosomal integration
For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364157.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06562 pHIS3H DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06562z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14201_A6Fnp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH-Forward RDB14201_A6Fna.seq
>06562_14201_A6Fn_ADH-Forward_A11_02.ab1
    1 TAGTCATTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
   61 GTGTTGGCAG CGATGAAATT ATTGACTCCT TAATTCGCAT TTCTTGTATT CCTGGAAAAG
  121 ATAAGATTTT GATGTGTCCA CCTTCGTACG GAATGTATAC GGTCTCTGCA AAAATCAACG
  181 ATGTTGAGGT TGTTAAGGTT CTCCTTGAAC CAGATTTCAA TTTGAACGTC GATGCTATTT
  241 GCGAAACACT TTCTAAAGAT AGTGCCATCA AAGTCTTTTT TGCTTGTTCC CCTGGTAATC
  301 CAACAGCGGC CGCGGGCCCG CGGCCGCTCT TAAACTCGAG GATATTAAGA AGATTTTGGA
  361 GCACCCTACA TGGAATGGAA TCGTTGTCGT CGATGAGGCC TATATTGATT TCTCTGCTCC
  421 CGATATGTCT GCCTTAACTC TTGTCAATGA ATATCCAAAC CTTGCTGTTT GCCAAACCCT
  481 TTCGAAATCT TTCGGTCTAG CAGGAATTCG GTAAGTTAAT CTAAGCTTTT AAGCATTAAT
  541 ATTATTTCTT TACTAACTAT GATGTTTAGT ATCGGATTTT GTTTGACAAG TAAACCTATT
  601 GCTACCATTA TGAACTCATT AAAGGCTCCT TATAACATTA GATCTGTTTA GCTTGCCTCG
  661 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT
  721 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC
  781 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC
  841 GGCTCCTCGC TGCGGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT
  901 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC
  961 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT
 1021 AAACAACCAT GGGTAAAAAG CCTGAACTCA CGCGACGTCT GTCGAGAAGT TTTCTGATCG
 1081 AAAAGTTCGA CAGCGTCTCC GAACTTGAAT GCAGCTCTCG AGGGCGAGAT CTCGAGCTTT
1141 CAGCCTTCCG GAAT
//
Primer: TEFpro_F RDB14201_A6Fnb.seq
>06562_14201_A6Fn_TEFpro_F_B11_05.ab1
    1 TCTGCCCGGG CGCGAAGCAA AAATTACGGC TCCTCGCTGC GGACCTGCGA GCAGGGAAAC
   61 GCTCCCCTCA CAGACGCGTT GAATTGTCCC CACGCCGCGC CCCTGTAGAG AAATATAAAA
  121 GGTTAGGATT TGCCACTGAG GTTCTTCTTT CATATACTTC CTTTTAAAAT CTTGCTAGGA
  181 TACAGTTCTC ACATCACATC CGAACATAAA CAACCATGGG TAAAAAGCCT GAACTCACCG
  241 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC
  301 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC
  361 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG
  421 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA
  481 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC
  541 TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA
  601 GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC
  661 GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG
  721 ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT
  781 GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA
  841 ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG
  901 AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT
  961 ACTTCGAGCG GGAGGCATCC GGAGCTTTGC AAGATCGCCG GCGGCTCCGG GCGGTATATG
 1021 CTCCGCATTG GGTCTTGACC CAACTTCTAT CAGAGCTTGG GTTTGACGGC AATTTTCGAA
 1081 TGATTGCCAG CTTTGGGGCG CAGGTCCGAT GCGAACGCAA TTCGTCCGGA ATTCGAAGTC
1141 GGGTACCTGT CGGGCGTTAC AC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

2018.12.10

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