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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHIS3H

S. pombe vector for chromosomal integration

Catalog number RDB06562
Resource name pHIS3H
Clone info. S. pombe vector for chromosomal integration
Comment For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364157.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008)の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06562 pHIS3H DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06562z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14201_A6Fnp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40polyA_R2
            Region: Zeo,SV40pro_ori
            Sequence file: RDB17487_A9I6j.seq check
            1
            Primer: TEFpro_F
            Sequence file: RDB14201_A6Fnb.seq check
            >06562_14201_A6Fn_TEFpro_F_B11_05.ab1
                1 TCTGCCCGGG CGCGAAGCAA AAATTACGGC TCCTCGCTGC GGACCTGCGA GCAGGGAAAC
               61 GCTCCCCTCA CAGACGCGTT GAATTGTCCC CACGCCGCGC CCCTGTAGAG AAATATAAAA
              121 GGTTAGGATT TGCCACTGAG GTTCTTCTTT CATATACTTC CTTTTAAAAT CTTGCTAGGA
              181 TACAGTTCTC ACATCACATC CGAACATAAA CAACCATGGG TAAAAAGCCT GAACTCACCG
              241 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC
              301 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC
              361 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG
              421 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA
              481 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC
              541 TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA
              601 GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC
              661 GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG
              721 ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT
              781 GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA
              841 ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG
              901 AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT
              961 ACTTCGAGCG GGAGGCATCC GGAGCTTTGC AAGATCGCCG GCGGCTCCGG GCGGTATATG
             1021 CTCCGCATTG GGTCTTGACC CAACTTCTAT CAGAGCTTGG GTTTGACGGC AATTTTCGAA
             1081 TGATTGCCAG CTTTGGGGCG CAGGTCCGAT GCGAACGCAA TTCGTCCGGA ATTCGAAGTC
            1141 GGGTACCTGT CGGGCGTTAC AC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

            2022.05.18

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