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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHIS3H

S. pombe vector for chromosomal integration

Catalog number RDB06562
Resource name pHIS3H
Clone info. S. pombe vector for chromosomal integration
For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364157.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06562 pHIS3H DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB06562z.seq

Original reference

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14201_A6Fnp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40polyA_R2
Region: Zeo,SV40pro_ori
Sequence file: RDB17487_A9I6j.seq check
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Primer: TEFpro_F
Sequence file: RDB14201_A6Fnb.seq check
>06562_14201_A6Fn_TEFpro_F_B11_05.ab1
    1 TCTGCCCGGG CGCGAAGCAA AAATTACGGC TCCTCGCTGC GGACCTGCGA GCAGGGAAAC
   61 GCTCCCCTCA CAGACGCGTT GAATTGTCCC CACGCCGCGC CCCTGTAGAG AAATATAAAA
  121 GGTTAGGATT TGCCACTGAG GTTCTTCTTT CATATACTTC CTTTTAAAAT CTTGCTAGGA
  181 TACAGTTCTC ACATCACATC CGAACATAAA CAACCATGGG TAAAAAGCCT GAACTCACCG
  241 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC
  301 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC
  361 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG
  421 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA
  481 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC
  541 TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA
  601 GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC
  661 GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG
  721 ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT
  781 GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA
  841 ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG
  901 AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT
  961 ACTTCGAGCG GGAGGCATCC GGAGCTTTGC AAGATCGCCG GCGGCTCCGG GCGGTATATG
 1021 CTCCGCATTG GGTCTTGACC CAACTTCTAT CAGAGCTTGG GTTTGACGGC AATTTTCGAA
 1081 TGATTGCCAG CTTTGGGGCG CAGGTCCGAT GCGAACGCAA TTCGTCCGGA ATTCGAAGTC
1141 GGGTACCTGT CGGGCGTTAC AC
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Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

2021.03.25

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