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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pARG1B

S. pombe vector for chromosomal integration

Catalog number RDB06559
Resource name pARG1B
Clone info. S. pombe vector for chromosomal integration
Comment For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364162.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008)の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06559 pARG1B DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06559z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14296_A6Hhp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CYC1ter_R
            Sequence file: RDB14296_A6Hha.seq check
            >06559_14296_A6Hh_CYC1ter_R_G01_19_ABI24.ab1
                1 ATGCAGTTTC GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG ACATAACTAA
               61 TTACATGATA TCGACAAAGG AAAAGGGGGA CGGATCTCCG AGGCCTCGGA CCCGTCGGGC
              121 CGCGTCGGAC GTGTCAGTCC TGCTCCTCGG CCACGAAGTG CTTAGCCCTC CCACACATAA
              181 CCAGAGGGCA GCAATTCACG AATCCCAACT GCCGTCGGCT GTCCATCACT GTCCTTCACT
              241 ATGGCTTTGA TCCCAGGATG CAGATCGAGA AGCACCTGTC GGCACCGTCC GCAGGGGCTC
              301 AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG TCAGGTTGCC AGCTGCCGCA
              361 GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCACAAGGTC CCCCAGTAAA ATGATATACA
              421 TTGACACCAG TGAAGATGCG GCCGTCGCTA GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT
              481 TCAGAGATGG GGATGCTGTT GATTGTAGCC GTTGCTCTTT CAATGAGGGT GGATTCTTCT
              541 TGAGACAAAG GCTTGGCCAT GGTTTAGTTC CTCACCTTGT CGTATTATAC TATGCCGATA
              601 TACTATGCCG ATGATTAATT GTCAACGCCG CCCTTAGATT AGATTGCTAT GCTTTCTTTC
              661 TAATGAGCAA GAAGTAAAAA AAGTTGTAAT AGAACAAGAA AAATGAAACT GAAACTTGAG
              721 AAATTGAAGA CCGTTTATTA ACTTAAATAT CAATGGGAGG TCATCGAAAG AGAAAAAAAT
              781 CAAAAAAAAA AATTTTCAAG AAAAAGAAAC GTGATAAAAA TTTTTATTGC CTTTTTCGAC
              841 GAAGAAAAAG AAACGAGGCG GTCTCTTTTT TCTTTTCCAA ACCTTTAGTA CGGGTAATTA
              901 ACGACACCCT AGAGGAAGAA AGAGGGGAAA TTTAGTATGC TGTGCTTGGG TGTTTTGAAG
              961 TGGTACGGCG ATGCGCGGGA GTCCGAGAAA ATCTGGGAAG AGTAAAAAAA AGGAGTAGAA
             1021 CATTTTTGAA GCTATGGGTG GTGGTGGGGC TAGATCTGCG ACCAAGTTCC GCATTTGAAT
            1081 TCCATCATAG GATGAAGGAG GGCACCAAAC GTTATTCCAC AGGCC
            //
            Primer: TEFpro_R
            Sequence file: RDB14296_A6Hhb.seq check
            >06559_14296_A6Hh_TEFpro_R_C01_07_ABI24.ab1
                1 GGGCAGGCGA GCGCGGAGTC GAGAAATCTG GAAGAGTAAA AAAGGAGTAG AAACATTTTG
               61 AAGCTATGGT GTGTGGGCTA GATCTGCGAC CAAGTCCGCA TTGAATTTCA TCATAGATTA
              121 AAGAAGCACC AACCTTATCA CAGGCCTTGC GCAAAGCAAT CAAAAATTCA GGCTTGGCAG
              181 GACAAATACC ACCTTCACCT TGAACGGGCT CGACAATAAC AGCCGCAGTT TTGTCATTGA
              241 CAAATTGCTC TATGGATGCA GGGTCATTAT AGACGGCTTG GACGACATCA GGAAGTAATG
              301 GTTGGAAACC ACGTTTATAT TTGGGGTTCG CAGTGATACT AAGAGATCCC AAAGAACGGC
              361 CGTGGAAAGA GTTATTAAAG TAAACAATTT GACTCTTCCC TTCTCCATAC TTCTCAAACG
              421 CGGCCGCGGG CCCGCGGCCG CCTTACGAGC GAACTTCAAG GCTGTCTCAT TGGCCTCAGT
              481 ACCACAATTG GCAAAGAAAA TTTTTGTGGG GCCAGCAATA CCGCTATTTT TTGCCAAAGA
              541 ATTATTAATG ACATTAGATA GCTCAATGGC AGGCTCGTTG TAGAAAAGGT TGCTACTATG
              601 GACTAATTTG GAGCATTGAT CAGCGGCAAG TCTAGCAACT TCAGGATGAG CATGACCCAA
              661 AGATGTAACA GCTACGCCAG AGGTGAAGTC AATGTATTTA CGACCTTCCT TGTCAAACAA
              721 ATAGGAGCCC TCACCTTTAG CAGCTACGAC TAGTATATTA CCCTGTTATC CCTAGCGGAT
              781 CTGCCGGTAG AGGTGTGGTC AATAAGAGCG ACCTCATACT ATACCTGAGA AAGCAACCTG
              841 ACCTACAGGA AAGAGTTACT CAAGAATAAG AATTTTCGTT TTAAAACCTA AGAGTCACTT
              901 TAAAATTTGT ATACACTTAT TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA
              961 TAAGAAATTC GCTTTATTTA GAAAGTGGGC GCGCCCGGAT CCTCTAGAGT CGACCCTGCA
             1021 GGCATGCAAA GCTTTGGCGT AATCATGGTC ATAGCTGTTC TTGTGTGAAA TGATATCCGC
            1081 TCACATCCAC ACAACAATAC GAGCCGGAAG CAATAAAAAG GTGGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

            2022.05.18

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