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pARG1B

S. pombe vector for chromosomal integration

Catalog number RDB06559
Resource name pARG1B
Clone info. S. pombe vector for chromosomal integration
For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364162.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06559 pARG1B DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06559z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14296_A6Hhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CYC1ter_R RDB14296_A6Hha.seq
>06559_14296_A6Hh_CYC1ter_R_G01_19_ABI24.ab1
    1 ATGCAGTTTC GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG ACATAACTAA
   61 TTACATGATA TCGACAAAGG AAAAGGGGGA CGGATCTCCG AGGCCTCGGA CCCGTCGGGC
  121 CGCGTCGGAC GTGTCAGTCC TGCTCCTCGG CCACGAAGTG CTTAGCCCTC CCACACATAA
  181 CCAGAGGGCA GCAATTCACG AATCCCAACT GCCGTCGGCT GTCCATCACT GTCCTTCACT
  241 ATGGCTTTGA TCCCAGGATG CAGATCGAGA AGCACCTGTC GGCACCGTCC GCAGGGGCTC
  301 AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG TCAGGTTGCC AGCTGCCGCA
  361 GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCACAAGGTC CCCCAGTAAA ATGATATACA
  421 TTGACACCAG TGAAGATGCG GCCGTCGCTA GAGAGAGCTG CGCTGGCGAC GCTGTAGTCT
  481 TCAGAGATGG GGATGCTGTT GATTGTAGCC GTTGCTCTTT CAATGAGGGT GGATTCTTCT
  541 TGAGACAAAG GCTTGGCCAT GGTTTAGTTC CTCACCTTGT CGTATTATAC TATGCCGATA
  601 TACTATGCCG ATGATTAATT GTCAACGCCG CCCTTAGATT AGATTGCTAT GCTTTCTTTC
  661 TAATGAGCAA GAAGTAAAAA AAGTTGTAAT AGAACAAGAA AAATGAAACT GAAACTTGAG
  721 AAATTGAAGA CCGTTTATTA ACTTAAATAT CAATGGGAGG TCATCGAAAG AGAAAAAAAT
  781 CAAAAAAAAA AATTTTCAAG AAAAAGAAAC GTGATAAAAA TTTTTATTGC CTTTTTCGAC
  841 GAAGAAAAAG AAACGAGGCG GTCTCTTTTT TCTTTTCCAA ACCTTTAGTA CGGGTAATTA
  901 ACGACACCCT AGAGGAAGAA AGAGGGGAAA TTTAGTATGC TGTGCTTGGG TGTTTTGAAG
  961 TGGTACGGCG ATGCGCGGGA GTCCGAGAAA ATCTGGGAAG AGTAAAAAAA AGGAGTAGAA
 1021 CATTTTTGAA GCTATGGGTG GTGGTGGGGC TAGATCTGCG ACCAAGTTCC GCATTTGAAT
1081 TCCATCATAG GATGAAGGAG GGCACCAAAC GTTATTCCAC AGGCC
//
Primer: TEFpro_R RDB14296_A6Hhb.seq
>06559_14296_A6Hh_TEFpro_R_C01_07_ABI24.ab1
    1 GGGCAGGCGA GCGCGGAGTC GAGAAATCTG GAAGAGTAAA AAAGGAGTAG AAACATTTTG
   61 AAGCTATGGT GTGTGGGCTA GATCTGCGAC CAAGTCCGCA TTGAATTTCA TCATAGATTA
  121 AAGAAGCACC AACCTTATCA CAGGCCTTGC GCAAAGCAAT CAAAAATTCA GGCTTGGCAG
  181 GACAAATACC ACCTTCACCT TGAACGGGCT CGACAATAAC AGCCGCAGTT TTGTCATTGA
  241 CAAATTGCTC TATGGATGCA GGGTCATTAT AGACGGCTTG GACGACATCA GGAAGTAATG
  301 GTTGGAAACC ACGTTTATAT TTGGGGTTCG CAGTGATACT AAGAGATCCC AAAGAACGGC
  361 CGTGGAAAGA GTTATTAAAG TAAACAATTT GACTCTTCCC TTCTCCATAC TTCTCAAACG
  421 CGGCCGCGGG CCCGCGGCCG CCTTACGAGC GAACTTCAAG GCTGTCTCAT TGGCCTCAGT
  481 ACCACAATTG GCAAAGAAAA TTTTTGTGGG GCCAGCAATA CCGCTATTTT TTGCCAAAGA
  541 ATTATTAATG ACATTAGATA GCTCAATGGC AGGCTCGTTG TAGAAAAGGT TGCTACTATG
  601 GACTAATTTG GAGCATTGAT CAGCGGCAAG TCTAGCAACT TCAGGATGAG CATGACCCAA
  661 AGATGTAACA GCTACGCCAG AGGTGAAGTC AATGTATTTA CGACCTTCCT TGTCAAACAA
  721 ATAGGAGCCC TCACCTTTAG CAGCTACGAC TAGTATATTA CCCTGTTATC CCTAGCGGAT
  781 CTGCCGGTAG AGGTGTGGTC AATAAGAGCG ACCTCATACT ATACCTGAGA AAGCAACCTG
  841 ACCTACAGGA AAGAGTTACT CAAGAATAAG AATTTTCGTT TTAAAACCTA AGAGTCACTT
  901 TAAAATTTGT ATACACTTAT TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA
  961 TAAGAAATTC GCTTTATTTA GAAAGTGGGC GCGCCCGGAT CCTCTAGAGT CGACCCTGCA
 1021 GGCATGCAAA GCTTTGGCGT AATCATGGTC ATAGCTGTTC TTGTGTGAAA TGATATCCGC
1081 TCACATCCAC ACAACAATAC GAGCCGGAAG CAATAAAAAG GTGGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

2019.02.18

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