Resource data sheet
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pARG1H

S. pombe vector for chromosomal integration

Catalog number RDB06558
Resource name pARG1H
Clone info. S. pombe vector for chromosomal integration
For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364159.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06558 pARG1H DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06558z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14295_A6Hhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: TEFpro_F RDB14295_A6Hha.seq
>06558_14295_A6Hh_TEFpro_F_D01_10_ABI24.ab1
    1 TCCAGCGCGA GCAAATTACG GCTCCTCGCT GCGGACCTGC GAGCAGGGAA ACGCTCCCCT
   61 CACAGACGCG TTGAATTGTC CCCACGCCGC GCCCCTGTAG AGAAATATAA AAGGTTAGGA
  121 TTTGCCACTG AGGTTCTTCT TTCATATACT TCCTTTTAAA ATCTTGCTAG GATACAGTTC
  181 TCACATCACA TCCGAACATA AACAACCATG GGTAAAAAGC CTGAACTCAC CGCGACGTCT
  241 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG
  301 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA
  361 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC
  421 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC
  481 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT
  541 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG
  601 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC
  661 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC
  721 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA
  781 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC
  841 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG GATTCCCAAT ACGAGGTCGC
  901 CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA
  961 GCGGAGGCAT CCGGGAGCTT GCAGGATCGC CGCGGCTCCC GGGCGTATAT GCTCCCGCAT
 1021 TGGGTCTTGA CCCAACTCTA TCAGAGCTTT GCATGGACGG CAATTTCGAT GAATGCAGCC
1081 TGGGCGCAAG GTTCGATGCG ACGCCAATTC GATTCCGGA
//
Primer: TEFter_R RDB14295_A6Hhb.seq
>06558_14295_A6Hh_TEFter_R_B01_04_ABI24.ab1
    1 AACAAGGCTA CATTTTGATT AAAATAGAAC AACTACAATA TAAAAAAACT ATACAAATGA
   61 CAAGTTCTTG AAAACAAGAA TCTTTTTATT GTCAGTACTG ATTATTCCTT TGCCCTCGGA
  121 CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC ATCGGTCCAG
  181 ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG TCCCGGCTCC GGATCGGACG
  241 ATTGCGTCGC ATCGACCCTG CGCCCAAGCT GCATCATCGA AATTGCCGTC AACCAAGCTC
  301 TGATAGAGTT GGTCAAGACC AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA
  361 AGCTCCGGAT GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC
  421 CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC CTCGCTCCAG
  481 TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT TGTTGGAGCC GAAATCCGCG
  541 TGCACGAGGT GCCGGACTTC GGGGCAGTCC TCGGCCCAAA GCATCAGCTC ATCGAGAGCC
  601 TGCGCGACGG ACGCACTGAC GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA
  661 TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG
  721 AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC CATGGCCTCC
  781 GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA CGTGACACCC
  841 TGTGCACGGC GGGAGATGCA ATAGGTCACG CTCTCGCTGA ATTCCCCAAT GTCAAGCACT
  901 TCCGGAAATC GGGAGCGCGG CCGATGCAAA GTGCCGATAA ACATAACGAT CTTTGTAGAA
  961 ACCATCGGCG CAGCTATTTA CCCGCAGGAC ATATCCACGC CCTCCTACAT CGAAAGCTGA
 1021 AAGCACGAGA TTCTTCGCCC TCGAGAGCTG CATCAGGTCG GAGACGCTGG TCGGACTTTT
1081 TCGATCAGAA AGATTCCTCC GAC
//
Primer: ADH-Forward RDB14295_A6Hhc.seq
>06558_14295_A6Hh_ADH_Forward_F01_16_ABI24.ab1
    1 TACTCCTATT GACACACCTC TACCGGCAGA TCCGCTAGGG ATAACAGGGT AATATACTAG
   61 TCGTAGCTGC TAAAGGTGAG GGCTCCTATT TGTTTGACAA GGAAGGTCGT AAATACATTG
  121 ACTTCACCTC TGGCGTAGCT GTTACATCTT TGGGTCATGC TCATCCTGAA GTTGCTAGAC
  181 TTGCCGCTGA TCAATGCTCC AAATTAGTCC ATAGTAGCAA CCTTTTCTAC AACGAGCCTG
  241 CCATTGAGCT ATCTAATGTC ATTAATAATT CTTTGGCAAA AAATAGCGGT ATTGCTGGCC
  301 CCACAAAAAT TTTCTTTGCC AATTGTGGTA CTGAGGCCAA TGAGACAGCC TTGAAGTTCG
  361 CTCGTAAGGC GGCCGCGGGC CCGCGGCCGC GTTTGAGAAG TATGGAGAAG GGAAGAGTCA
  421 AATTGTTTAC TTTAATAACT CTTTCCACGG CCGTTCTTTG GGATCTCTTA GTATCACTGC
  481 GAACCCCAAA TATAAACGTG GTTTCCAACC ATTACTTCCT GATGTCGTCC AAGCCGTCTA
  541 TAATGACCCT GCATCCATAG AGCAATTTGT CAATGACAAA ACTGCGGCTG TTATTGTCGA
  601 GCCCGTTCAA GGTGAAGGTG GTATTTGTCC TGCCAAGCCT GAATTTTTGA TTGCTTTGCG
  661 CAAGGCCTGT GATAAGGTTG GTGCTTCTTT AATCTATGAT GAAATTCAAT GCGGACTTGG
  721 TCGCAGATCT GTTTAGCTTG CCTCGTCCCC GCCGGGTCAC CCGGCCAGCG ACATGGAGGC
  781 CCAGAATACC CTCCTTGACA GTCTTGACGT GCGCAGCTCA GGGCATGATG TGACTGTCGC
  841 CCGTACATTT AGCCCATACA TCCCCATGTA TAATCATTTG CATCCATACA TTTTGATGGC
  901 CGCACGGCGC GAAGCAAAAA ATTACGGCTC CTCGCTGCGG ACCTGCGAGC AGGGAAACGC
  961 TCCCCTCACA GACGCGTTTG AATTGTCCCC ACGCCGCGCC CCTGTAGAGA ATATAAAAGG
 1021 TTAAGATTTG CACTGAGTTC TCTTTCATAT ACTCTTTTAA AATCTTGCTA GATACAGCTC
1081 TCACATCACA TCCGCAACAT ACATCATGTT CAAAGGCCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

2018.12.10

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