RDB06558 - pARG1H

Resource data sheet
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pARG1H

S. pombe vector for chromosomal integration

Catalog number	RDB06558
Resource name	pARG1H
Clone info.	S. pombe vector for chromosomal integration For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone	pUC119 (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364159.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008)の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06558	pARG1H	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06558z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14295_A6Hhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: TEFpro_F	RDB14295_A6Hha.seq
>06558_14295_A6Hh_TEFpro_F_D01_10_ABI24.ab1 1 TCCAGCGCGA GCAAATTACG GCTCCTCGCT GCGGACCTGC GAGCAGGGAA ACGCTCCCCT 61 CACAGACGCG TTGAATTGTC CCCACGCCGC GCCCCTGTAG AGAAATATAA AAGGTTAGGA 121 TTTGCCACTG AGGTTCTTCT TTCATATACT TCCTTTTAAA ATCTTGCTAG GATACAGTTC 181 TCACATCACA TCCGAACATA AACAACCATG GGTAAAAAGC CTGAACTCAC CGCGACGTCT 241 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG 301 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA 361 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC 421 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC 481 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT 541 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG 601 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC 661 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC 721 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA 781 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC 841 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG GATTCCCAAT ACGAGGTCGC 901 CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA 961 GCGGAGGCAT CCGGGAGCTT GCAGGATCGC CGCGGCTCCC GGGCGTATAT GCTCCCGCAT 1021 TGGGTCTTGA CCCAACTCTA TCAGAGCTTT GCATGGACGG CAATTTCGAT GAATGCAGCC 1081 TGGGCGCAAG GTTCGATGCG ACGCCAATTC GATTCCGGA //
Primer: TEFter_R	RDB14295_A6Hhb.seq
>06558_14295_A6Hh_TEFter_R_B01_04_ABI24.ab1 1 AACAAGGCTA CATTTTGATT AAAATAGAAC AACTACAATA TAAAAAAACT ATACAAATGA 61 CAAGTTCTTG AAAACAAGAA TCTTTTTATT GTCAGTACTG ATTATTCCTT TGCCCTCGGA 121 CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC ATCGGTCCAG 181 ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG TCCCGGCTCC GGATCGGACG 241 ATTGCGTCGC ATCGACCCTG CGCCCAAGCT GCATCATCGA AATTGCCGTC AACCAAGCTC 301 TGATAGAGTT GGTCAAGACC AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA 361 AGCTCCGGAT GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC 421 CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC CTCGCTCCAG 481 TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT TGTTGGAGCC GAAATCCGCG 541 TGCACGAGGT GCCGGACTTC GGGGCAGTCC TCGGCCCAAA GCATCAGCTC ATCGAGAGCC 601 TGCGCGACGG ACGCACTGAC GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA 661 TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG 721 AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC CATGGCCTCC 781 GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA CGTGACACCC 841 TGTGCACGGC GGGAGATGCA ATAGGTCACG CTCTCGCTGA ATTCCCCAAT GTCAAGCACT 901 TCCGGAAATC GGGAGCGCGG CCGATGCAAA GTGCCGATAA ACATAACGAT CTTTGTAGAA 961 ACCATCGGCG CAGCTATTTA CCCGCAGGAC ATATCCACGC CCTCCTACAT CGAAAGCTGA 1021 AAGCACGAGA TTCTTCGCCC TCGAGAGCTG CATCAGGTCG GAGACGCTGG TCGGACTTTT 1081 TCGATCAGAA AGATTCCTCC GAC //
Primer: ADH-Forward	RDB14295_A6Hhc.seq
>06558_14295_A6Hh_ADH_Forward_F01_16_ABI24.ab1 1 TACTCCTATT GACACACCTC TACCGGCAGA TCCGCTAGGG ATAACAGGGT AATATACTAG 61 TCGTAGCTGC TAAAGGTGAG GGCTCCTATT TGTTTGACAA GGAAGGTCGT AAATACATTG 121 ACTTCACCTC TGGCGTAGCT GTTACATCTT TGGGTCATGC TCATCCTGAA GTTGCTAGAC 181 TTGCCGCTGA TCAATGCTCC AAATTAGTCC ATAGTAGCAA CCTTTTCTAC AACGAGCCTG 241 CCATTGAGCT ATCTAATGTC ATTAATAATT CTTTGGCAAA AAATAGCGGT ATTGCTGGCC 301 CCACAAAAAT TTTCTTTGCC AATTGTGGTA CTGAGGCCAA TGAGACAGCC TTGAAGTTCG 361 CTCGTAAGGC GGCCGCGGGC CCGCGGCCGC GTTTGAGAAG TATGGAGAAG GGAAGAGTCA 421 AATTGTTTAC TTTAATAACT CTTTCCACGG CCGTTCTTTG GGATCTCTTA GTATCACTGC 481 GAACCCCAAA TATAAACGTG GTTTCCAACC ATTACTTCCT GATGTCGTCC AAGCCGTCTA 541 TAATGACCCT GCATCCATAG AGCAATTTGT CAATGACAAA ACTGCGGCTG TTATTGTCGA 601 GCCCGTTCAA GGTGAAGGTG GTATTTGTCC TGCCAAGCCT GAATTTTTGA TTGCTTTGCG 661 CAAGGCCTGT GATAAGGTTG GTGCTTCTTT AATCTATGAT GAAATTCAAT GCGGACTTGG 721 TCGCAGATCT GTTTAGCTTG CCTCGTCCCC GCCGGGTCAC CCGGCCAGCG ACATGGAGGC 781 CCAGAATACC CTCCTTGACA GTCTTGACGT GCGCAGCTCA GGGCATGATG TGACTGTCGC 841 CCGTACATTT AGCCCATACA TCCCCATGTA TAATCATTTG CATCCATACA TTTTGATGGC 901 CGCACGGCGC GAAGCAAAAA ATTACGGCTC CTCGCTGCGG ACCTGCGAGC AGGGAAACGC 961 TCCCCTCACA GACGCGTTTG AATTGTCCCC ACGCCGCGCC CCTGTAGAGA ATATAAAAGG 1021 TTAAGATTTG CACTGAGTTC TCTTTCATAT ACTCTTTTAA AATCTTGCTA GATACAGCTC 1081 TCACATCACA TCCGCAACAT ACATCATGTT CAAAGGCCG //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

2018.12.10

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