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pARG1K

S. pombe vector for chromosomal integration

Catalog number RDB06557
Resource name pARG1K
Clone info. S. pombe vector for chromosomal integration
For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364153.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06557 pARG1K DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06557z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14294_A6Hhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: TEFter_R RDB14294_A6Hha.seq
>06557_14294_A6Hh_TEFter_R_A01_01_ABI24.ab1
    1 AATCACGCTA CATTTGATTA AAATAGAACA ACTACAATAT AAAAAAACTA TACAAATGAC
   61 AAGTTCTTGA AAACAAGAAT CTTTTTATTG TCAGTACTGA TTAGAAAAAC TCATCGAGCA
  121 TCAAATGAAA CTGCAATTTA TTCATATCAG GATTATCAAT ACCATATTTT TGAAAAAGCC
  181 GTTTCTGTAA TGAAGGAGAA AACTCACCGA GGCAGTTCCA TAGGATGGCA AGATCCTGGT
  241 ATCGGTCTGC GATTCCGACT CGTCCAACAT CAATACAACC TATTAATTTC CCCTCGTCAA
  301 AAATAAGGTT ATCAAGTGAG AAATCACCAT GAGTGACGAC TGAATCCGGT GAGAATGGCA
  361 AAAGCTTATG CATTTCTTTC CAGACTTGTT CAACAGGCCA GCCATTACGC TCGTCATCAA
  421 AATCACTCGC ATCAACCAAA CCGTTATTCA TTCGTGATTG CGCCTGAGCG AGACGAAATA
  481 CGCGATCGCT GTTAAAAGGA CAATTACAAA CAGGAATCGA ATGCAACCGG CGCAGGAACA
  541 CTGCCAGCGC ATCAACAATA TTTTCACCTG AATCAGGATA TTCTTCTAAT ACCTGGAATG
  601 CTGTTTTGCC GGGGATCGCA GTGGTGAGTA ACCATGCATC ATCAGGAGTA CGGATAAAAT
  661 GCTTGATGGT CGGAAGAGGC ATAAATTCCG TCAGCCAGTT TAGTCTGACC ATCTCATCTG
  721 TAACATCATT GGCAACGCTA CCTTTGCCAT GTTTCAGAAA CAACTCTGGC GCATCGGGCT
  781 TCCCATACAA TCGATAGATT GTCGCACCTG ATTGCCCGAC ATTATCGCGA GCCCATTTAT
  841 ACCCATATAA ATCAGCATCC ATGTTGGAAT TTAATCGCGG CCTCGAAACG TGAGTCTTTT
  901 CCTTACCCAT GGGTTGTTTA TGTTCGGATG TGATGTGAGA ACTGTATCCT AGCAAGATTT
  961 TAAAACGAAG TATATGAAAG AAGAACCTCA GTGGCAATCC CTAACCTTTT TATATTTCTC
 1021 CTACAGGGGC CGCCGCCGTT GGGAACCATC CACGCGTCCT GTGAGGAGCG TTGCCTGCCT
1081 CGCAAGGTCC TGCAGCGGAA GA
//
Primer: ADH-Forward RDB14294_A6Hhb.seq
>06557_14294_A6Hh_ADH_Forward_C01_07_ABI24.ab1
    1 TACTCCTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
   61 GTCGTAGCTG CTAAAGGTGA GGGCTCCTAT TTGTTTGACA AGGAAGGTCG TAAATACATT
  121 GACTTCACCT CTGGCGTAGC TGTTACATCT TTGGGTCATG CTCATCCTGA AGTTGCTAGA
  181 CTTGCCGCTG ATCAATGCTC CAAATTAGTC CATAGTAGCA ACCTTTTCTA CAACGAGCCT
  241 GCCATTGAGC TATCTAATGT CATTAATAAT TCTTTGGCAA AAAATAGCGG TATTGCTGGC
  301 CCCACAAAAA TTTTCTTTGC CAATTGTGGT ACTGAGGCCA ATGAGACAGC CTTGAAGTTC
  361 GCTCGTAAGG CGGCCGCGGG CCCGCGGCCG CGTTTGAGAA GTATGGAGAA GGGAAGAGTC
  421 AAATTGTTTA CTTTAATAAC TCTTTCCACG GCCGTTCTTT GGGATCTCTT AGTATCACTG
  481 CGAACCCCAA ATATAAACGT GGTTTCCAAC CATTACTTCC TGATGTCGTC CAAGCCGTCT
  541 ATAATGACCC TGCATCCATA GAGCAATTTG TCAATGACAA AACTGCGGCT GTTATTGTCG
  601 AGCCCGTTCA AGGTGAAGGT GGTATTTGTC CTGCCAAGCC TGAATTTTTG ATTGCTTTGC
  661 GCAAGGCCTG TGATAAGGTT GGTGCTTCTT TAATCTATGA TGAAATTCAA TGCGGACTTG
  721 GTCGCAGATC TGTTTAGCTT GCCTCGTCCC CGCCGGGTCA CCCGGCCAGC GACATGGAGG
  781 CCCAGAATAC CCTCCTTGAC AGTCTTGACG TGCGCAGCTC AGGGCATGAT GTGACTGTCG
  841 CCCGTACATT TAGCCCATAC ATCCCCATGT ATAATCATTT GCATCCATAC ATTTTGATGG
  901 CCGCACGGCG CGAAGCAAAA ATTACGGCTC CTCGCTGCAG ACCTGCGAGC AGGGAAACGC
  961 TTCCCCTCAC AAGACGCGTT GATGTCCACG GCGCGCCTGT AGAGAAAATA TAAAAGTAAG
 1021 AATTGGCAAC TGAGATCCTC ATCATATACC TCGTTTAAAT CTGCTAGCAT CAGTACTCAC
1081 ATCACACTTC CG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753 .

2018.12.10

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