RDB06557 - pARG1K

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pARG1K (#RDB06557)

S. pombe vector for chromosomal integration

Clone info.	S. pombe vector for chromosomal integration
Comment	For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone	pUC119 (Plasmid)
Selectable markers	Amp^r
Reference of insert sequence	AB364153.1 (DDBJ accession number)
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06557z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008)の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008)の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06557	pARG1K	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pARG1K was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06557).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]

Matsuyama, A,, Shirai, A,, Yoshida, M., A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14294_A6Hhp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: TEFter_R Sequence file: RDB14294_A6Hha.seq
>06557_14294_A6Hh_TEFter_R_A01_01_ABI24.ab1 1 AATCACGCTA CATTTGATTA AAATAGAACA ACTACAATAT AAAAAAACTA TACAAATGAC 61 AAGTTCTTGA AAACAAGAAT CTTTTTATTG TCAGTACTGA TTAGAAAAAC TCATCGAGCA 121 TCAAATGAAA CTGCAATTTA TTCATATCAG GATTATCAAT ACCATATTTT TGAAAAAGCC 181 GTTTCTGTAA TGAAGGAGAA AACTCACCGA GGCAGTTCCA TAGGATGGCA AGATCCTGGT 241 ATCGGTCTGC GATTCCGACT CGTCCAACAT CAATACAACC TATTAATTTC CCCTCGTCAA 301 AAATAAGGTT ATCAAGTGAG AAATCACCAT GAGTGACGAC TGAATCCGGT GAGAATGGCA 361 AAAGCTTATG CATTTCTTTC CAGACTTGTT CAACAGGCCA GCCATTACGC TCGTCATCAA 421 AATCACTCGC ATCAACCAAA CCGTTATTCA TTCGTGATTG CGCCTGAGCG AGACGAAATA 481 CGCGATCGCT GTTAAAAGGA CAATTACAAA CAGGAATCGA ATGCAACCGG CGCAGGAACA 541 CTGCCAGCGC ATCAACAATA TTTTCACCTG AATCAGGATA TTCTTCTAAT ACCTGGAATG 601 CTGTTTTGCC GGGGATCGCA GTGGTGAGTA ACCATGCATC ATCAGGAGTA CGGATAAAAT 661 GCTTGATGGT CGGAAGAGGC ATAAATTCCG TCAGCCAGTT TAGTCTGACC ATCTCATCTG 721 TAACATCATT GGCAACGCTA CCTTTGCCAT GTTTCAGAAA CAACTCTGGC GCATCGGGCT 781 TCCCATACAA TCGATAGATT GTCGCACCTG ATTGCCCGAC ATTATCGCGA GCCCATTTAT 841 ACCCATATAA ATCAGCATCC ATGTTGGAAT TTAATCGCGG CCTCGAAACG TGAGTCTTTT 901 CCTTACCCAT GGGTTGTTTA TGTTCGGATG TGATGTGAGA ACTGTATCCT AGCAAGATTT 961 TAAAACGAAG TATATGAAAG AAGAACCTCA GTGGCAATCC CTAACCTTTT TATATTTCTC 1021 CTACAGGGGC CGCCGCCGTT GGGAACCATC CACGCGTCCT GTGAGGAGCG TTGCCTGCCT 1081 CGCAAGGTCC TGCAGCGGAA GA //
Primer: ADH-Forward Sequence file: RDB14294_A6Hhb.seq
>06557_14294_A6Hh_ADH_Forward_C01_07_ABI24.ab1 1 TACTCCTTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA 61 GTCGTAGCTG CTAAAGGTGA GGGCTCCTAT TTGTTTGACA AGGAAGGTCG TAAATACATT 121 GACTTCACCT CTGGCGTAGC TGTTACATCT TTGGGTCATG CTCATCCTGA AGTTGCTAGA 181 CTTGCCGCTG ATCAATGCTC CAAATTAGTC CATAGTAGCA ACCTTTTCTA CAACGAGCCT 241 GCCATTGAGC TATCTAATGT CATTAATAAT TCTTTGGCAA AAAATAGCGG TATTGCTGGC 301 CCCACAAAAA TTTTCTTTGC CAATTGTGGT ACTGAGGCCA ATGAGACAGC CTTGAAGTTC 361 GCTCGTAAGG CGGCCGCGGG CCCGCGGCCG CGTTTGAGAA GTATGGAGAA GGGAAGAGTC 421 AAATTGTTTA CTTTAATAAC TCTTTCCACG GCCGTTCTTT GGGATCTCTT AGTATCACTG 481 CGAACCCCAA ATATAAACGT GGTTTCCAAC CATTACTTCC TGATGTCGTC CAAGCCGTCT 541 ATAATGACCC TGCATCCATA GAGCAATTTG TCAATGACAA AACTGCGGCT GTTATTGTCG 601 AGCCCGTTCA AGGTGAAGGT GGTATTTGTC CTGCCAAGCC TGAATTTTTG ATTGCTTTGC 661 GCAAGGCCTG TGATAAGGTT GGTGCTTCTT TAATCTATGA TGAAATTCAA TGCGGACTTG 721 GTCGCAGATC TGTTTAGCTT GCCTCGTCCC CGCCGGGTCA CCCGGCCAGC GACATGGAGG 781 CCCAGAATAC CCTCCTTGAC AGTCTTGACG TGCGCAGCTC AGGGCATGAT GTGACTGTCG 841 CCCGTACATT TAGCCCATAC ATCCCCATGT ATAATCATTT GCATCCATAC ATTTTGATGG 901 CCGCACGGCG CGAAGCAAAA ATTACGGCTC CTCGCTGCAG ACCTGCGAGC AGGGAAACGC 961 TTCCCCTCAC AAGACGCGTT GATGTCCACG GCGCGCCTGT AGAGAAAATA TAAAAGTAAG 1021 AATTGGCAAC TGAGATCCTC ATCATATACC TCGTTTAAAT CTGCTAGCAT CAGTACTCAC 1081 ATCACACTTC CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original	Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753. [link to RRC of NBRP]

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