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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-GFH31

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter

Catalog number RDB06544
Resource name pDUAL-GFH31
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364132 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06544 pDUAL-GFH31 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06544z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14202_A6Fnp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB14202_A6Fna.seq check
            >06544_14202_A6Fn_o_2_ADH_rev_A12_03.ab1
                1 GGGGGTTCTC AGATAAGAAT TTTCGTTTTA AACCTAAGAG TCACTTTAAA ATTTGTATAC
               61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
              121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC AGTGGTGATG GTGGTGATGT
              181 TTGTCATCGT CGTCCTTGTA GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC
              241 CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC
              301 GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
              361 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT
              421 CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG
              481 TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA
              541 ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT
              601 GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC
              661 ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA
              721 ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG
              781 CAAATAAATT TAAGGGTAAG TTTTCCGTAT GTTGCATCAC CTTTCACCCT CTCCACTGAC
              841 AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA CAACTCCAGT
              901 GAAAAGTTCT TCTCCTTTAC TCATGATATC CGGGATCCAG GCCCTGTCGA CGCTAGCATC
              961 AGTAACTCTA AAGTCCCTTC TATTCCTTTT TTTTAACCCT TTATTTTTGG CGGCTAGTGT
             1021 TTTGCTCTAG TACGTCAAGT GCCTTGCAGA ATGAAGAACT ACGGTGTCGC ATCTAATGCT
            1081 GGCAGTCCTA CCATCACAGT GAATGTCACG ATCATGGAAG TTTATTTGAC
            //
            Primer: LEU1pro_F
            Sequence file: RDB14202_A6Fnb.seq check
            >06544_14202_A6Fn_o_2_LEU1pro_F_B12_06.ab1
                1 ATAAAGTGCA CAGTGTTGAG TTTCCCGAAA CCAGGCATTT TTGCGGTAGG GTCTGAAACT
               61 TTCACGAAAC CATTTCACAA CAAAACTTCT TCAACAACAT GTGTGCAAAG AAAATCGTCG
              121 TCTTACCAGG AGACCATATT GGCCCTGAAA TTGTTGCTTC TGCCTTGGAG GTTTTGAAAG
              181 TCGTTGAGAA GAAGCGACCT GAGTTAAAAC TCGAGTTTGA AGAACACAAG ATTGGAGGTG
              241 CCTCTATTGA TGCCTATGGA ACCCCTTTGA CTGACGAGAC TGTGAAGGCT TGTTTGGAAG
              301 CTGACGGTGT TCTTTTGGGT GCCGTTGGTG GTCCTGAATG GACCAACCCC AATTGTCGTC
              361 CTGAGCAAGG TTTATTGAAG CTTCGTAAGA GTATGGGTGT TTGGGCCAAC CTTCGACCTT
              421 GCAACTTTGC CAGCAAGTCT TTAGTCAAGT ACAGCCCTTT GAAGCCTGAA ATCGTTGAAG
              481 GTGTCGATTT TTGTGTTGTA CGAGAACTTA CTGGAGGTTG TTACTTTGGT GAGCGCACTG
              541 AGGACAACGG ATCGGGTTAT GCTATGGACA CTTGGCCTTA CAGTTTGGAA GAAGTTTCTC
              601 GTATTGCTCG TTTGGCGGCC GCGGGCCCAA CACCAATGTT TATAACCAAG TTTTATCTTG
              661 TTTGTCTACA TGGTATTTTA CATTCATCTA CATACATCTT TCATTGGCTT TGTACATAGT
              721 TATCATTACA AGTCTAAAAA AATTCACTCT TTTCTTATTC AATGTCAATC CAAGAGAAAA
              781 GATTGTGGTA ATGTTGTAGG AGCATGTTTA ATAAATTACT ATAGCAAATT ACTTTTTATT
              841 CCCAAGGTGT TTATCTATAA TAGTTAATAT TTTAGTCGCT ACATAAAATT TTACCAAAGA
              901 GTACTTGTAT ACTAATTCTA AATGCCTTCT GACATAAAAC GCCTAGGAAA ACAAACGCAA
              961 ACAACGCATC GACTTTTTCA ATAACCAACC AAAAAATTTT ACATTAGTCT TTTTTTAATG
             1021 CTGAGAAAGT CTTTGCTGAT ATGCCCTTCC ACAGCCTTCC TCTATATCTC TTGGCCTCCG
             1081 ACCACAGAAT TACGAACAGC TCATAGACTT CCACGAACGA CATTGATGAA TGGTCGGCTT
            1141 ACCGTCAGTT TAACAAATCC ACCGATC
            //
            Primer: f1ori_R3
            Sequence file: RDB14202_A6Fnc.seq check
            >06544_14202_A6Fn_o_2_f1ori_R3_C12_09.ab1
                1 GGGGTCGCTG AGTATGCGGT GTGAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC
               61 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT
              121 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG
              181 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGGAGC
              241 GCTACCGTGA ATGGGCTCAA CCAAACAATG AACCTTTTCT TCTGATTTTC CTACCACACC
              301 GGAAAGGGAG GCAGAAGGCA AAAGGCCCAA GCTACCAGGA ATGACAGAAG CCTCATCTGA
              361 AATAATGTCA CCAAACAAGT TGTCAGTCAA AACAACACCG TTAAGTGTAC GAGGGCTCTT
              421 GACCAAAAGC ATGGCTGCGG AGTCAATGAG CTGGTTTTTT AAGGTAAGGT GAGGATATTC
              481 CTCCTTAAAA ATCTTAGCTA CAGTCTTGCG CCAAAGACGA GAAGTTGCCA AAACATTAGC
              541 TTTGTCGAGT AATGTGACGG GAGCAGGAGG GTTGGAAGTT TCAGCTAAGC GGCCGCGGGC
              601 CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG
              661 AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA
              721 ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG
              781 ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC
              841 CGAAATGTAA AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT
              901 AAAAAGTAGG GCAAGCGAGA CAGAATGGGA TACAAGGGCA TCGTCTATAA TTATAGCTAA
              961 AAATTGTATT TTAATTTGTA TTTTTTGTAA TTTTATTTCT CATTGTTTTA CTTAAAATGA
             1021 ATGCGAATTT AGAGAAAACT TTCACGAAAT GTCAAATAGC TCAGCAAAAT ATACCATTTT
             1081 AGGCAAGCGA TCAGCACTAT CTCGATGTAG ATCCTTTTCC TTCATAAATT TCCAACAACT
            1141 GTA
            //
            Primer: URA4pro_F
            Sequence file: RDB14202_A6Fnd.seq check
            >06544_14202_A6Fn_o_2_URA4pro_F_D12_12.ab1
                1 TTGTATCGTA CGTATTGTCC TACCAAGAAC CTCTTTTTTG CTTGGATCGA AATTAAAGGT
               61 TTAAAAGCAA AGTTATGGAT GCTAGAGTAT TTCAAAGCTA TTCAGCTAGA GCTGAGGGGA
              121 TGAAAAATCC CATTGCCAAG GAATTGTTGG CTTTGATGGA AGAAAAGCAA AGCAACTTGT
              181 CAGTCGCGGT CGATTTGACG AAGAAATCCG AAATCTTAGA ATTGGTAGAT AAAATTGGAC
              241 CCTATGTCTG TGTTATCAAG ACACATATTG ACGTTGTCGA GGATTTCGAC CAGGATATGG
              301 TAGAAAAACT GGTGGCCTTA GGTAAAAAGC ATCGTTTTCT TATCTTTGAG GATCGCAAAT
              361 TCGCAGACAT TGGAAATACC GTCAAGCTAC AATATGCATC TGGTGTGTAC AAAATTGCTT
              421 CTTGGGCTCA TATCACAAAT TGCCATACAG TGCCAGGCGA GGGTATTATA CAAGGCCTCA
              481 AAGAAGTTGG TTTACCTTTG GGACGTGGTC TCTTGCTTTT GGCTGAAATG TCTTCCAAAG
              541 GCTCTTTGGC TACTGGTTCC TACACAGAGA AAACCTTAGA ATGGTTTGAG AAGCATACCG
              601 ATTTTTGCTT TGGCTTTATA GCTGGTCGTC GATTTCCTAA CCTTCAAAGC GACTACATAA
              661 CTATGTCCCC TGGTATCGGC TTGGATGTTA AAGGAGACGG GCTGGGACAG CAATATCGTA
              721 CTCCTGAAGA AGTGATTGTA AACTGCGGTA GCGACATCAT CATTGTTGGT CGTGGAGTCT
              781 ATGGAGCTGG TCGTAATCCT GTTGTCGAAG CCAAGAGATA TAGAGAAGCT GGTTGGAAGG
              841 CATATCAGCA AAGACTTTCT CAGCATTAAA AAAAGACTAA TGTAAAATTT TTTTTGGTTG
              901 GTTATTGAAA AAGTCGATGC CTTGTTTGCG TTTGTTTTCC TAGGCGTTTT ATGTCAGAAC
              961 GCATTTAGAA TTAGTATACA AGTACTCTTT GGGTAAAATT TTATGTAGCG ACTAAAATAT
             1021 TACTATTATA GATAAACACC TTTGGAATAA AAAGTTATTG CTATAGTTAT TTATAAACAT
             1081 GCTTCTTACA ACATAACACA TCGTTCTCAT GTATTGACAT TGAATAGAAA GAAGTGAGTT
            1141 TTTTTAGAAC TG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl