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pDUAL-GFH31

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter

Catalog number RDB06544
Resource name pDUAL-GFH31
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364132 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06544 pDUAL-GFH31 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06544z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14202_A6Fnp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev RDB14202_A6Fna.seq
>06544_14202_A6Fn_o_2_ADH_rev_A12_03.ab1
    1 GGGGGTTCTC AGATAAGAAT TTTCGTTTTA AACCTAAGAG TCACTTTAAA ATTTGTATAC
   61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
  121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC AGTGGTGATG GTGGTGATGT
  181 TTGTCATCGT CGTCCTTGTA GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC
  241 CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC
  301 GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
  361 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT
  421 CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG
  481 TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA
  541 ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT
  601 GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC
  661 ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA
  721 ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG
  781 CAAATAAATT TAAGGGTAAG TTTTCCGTAT GTTGCATCAC CTTTCACCCT CTCCACTGAC
  841 AGAAAATTTG TGCCCATTAA CATCACCATC TAATTCAACA AGAATTGGGA CAACTCCAGT
  901 GAAAAGTTCT TCTCCTTTAC TCATGATATC CGGGATCCAG GCCCTGTCGA CGCTAGCATC
  961 AGTAACTCTA AAGTCCCTTC TATTCCTTTT TTTTAACCCT TTATTTTTGG CGGCTAGTGT
 1021 TTTGCTCTAG TACGTCAAGT GCCTTGCAGA ATGAAGAACT ACGGTGTCGC ATCTAATGCT
1081 GGCAGTCCTA CCATCACAGT GAATGTCACG ATCATGGAAG TTTATTTGAC
//
Primer: LEU1pro_F RDB14202_A6Fnb.seq
>06544_14202_A6Fn_o_2_LEU1pro_F_B12_06.ab1
    1 ATAAAGTGCA CAGTGTTGAG TTTCCCGAAA CCAGGCATTT TTGCGGTAGG GTCTGAAACT
   61 TTCACGAAAC CATTTCACAA CAAAACTTCT TCAACAACAT GTGTGCAAAG AAAATCGTCG
  121 TCTTACCAGG AGACCATATT GGCCCTGAAA TTGTTGCTTC TGCCTTGGAG GTTTTGAAAG
  181 TCGTTGAGAA GAAGCGACCT GAGTTAAAAC TCGAGTTTGA AGAACACAAG ATTGGAGGTG
  241 CCTCTATTGA TGCCTATGGA ACCCCTTTGA CTGACGAGAC TGTGAAGGCT TGTTTGGAAG
  301 CTGACGGTGT TCTTTTGGGT GCCGTTGGTG GTCCTGAATG GACCAACCCC AATTGTCGTC
  361 CTGAGCAAGG TTTATTGAAG CTTCGTAAGA GTATGGGTGT TTGGGCCAAC CTTCGACCTT
  421 GCAACTTTGC CAGCAAGTCT TTAGTCAAGT ACAGCCCTTT GAAGCCTGAA ATCGTTGAAG
  481 GTGTCGATTT TTGTGTTGTA CGAGAACTTA CTGGAGGTTG TTACTTTGGT GAGCGCACTG
  541 AGGACAACGG ATCGGGTTAT GCTATGGACA CTTGGCCTTA CAGTTTGGAA GAAGTTTCTC
  601 GTATTGCTCG TTTGGCGGCC GCGGGCCCAA CACCAATGTT TATAACCAAG TTTTATCTTG
  661 TTTGTCTACA TGGTATTTTA CATTCATCTA CATACATCTT TCATTGGCTT TGTACATAGT
  721 TATCATTACA AGTCTAAAAA AATTCACTCT TTTCTTATTC AATGTCAATC CAAGAGAAAA
  781 GATTGTGGTA ATGTTGTAGG AGCATGTTTA ATAAATTACT ATAGCAAATT ACTTTTTATT
  841 CCCAAGGTGT TTATCTATAA TAGTTAATAT TTTAGTCGCT ACATAAAATT TTACCAAAGA
  901 GTACTTGTAT ACTAATTCTA AATGCCTTCT GACATAAAAC GCCTAGGAAA ACAAACGCAA
  961 ACAACGCATC GACTTTTTCA ATAACCAACC AAAAAATTTT ACATTAGTCT TTTTTTAATG
 1021 CTGAGAAAGT CTTTGCTGAT ATGCCCTTCC ACAGCCTTCC TCTATATCTC TTGGCCTCCG
 1081 ACCACAGAAT TACGAACAGC TCATAGACTT CCACGAACGA CATTGATGAA TGGTCGGCTT
1141 ACCGTCAGTT TAACAAATCC ACCGATC
//
Primer: f1ori_R3 RDB14202_A6Fnc.seq
>06544_14202_A6Fn_o_2_f1ori_R3_C12_09.ab1
    1 GGGGTCGCTG AGTATGCGGT GTGAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC
   61 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT
  121 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG
  181 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGGAGC
  241 GCTACCGTGA ATGGGCTCAA CCAAACAATG AACCTTTTCT TCTGATTTTC CTACCACACC
  301 GGAAAGGGAG GCAGAAGGCA AAAGGCCCAA GCTACCAGGA ATGACAGAAG CCTCATCTGA
  361 AATAATGTCA CCAAACAAGT TGTCAGTCAA AACAACACCG TTAAGTGTAC GAGGGCTCTT
  421 GACCAAAAGC ATGGCTGCGG AGTCAATGAG CTGGTTTTTT AAGGTAAGGT GAGGATATTC
  481 CTCCTTAAAA ATCTTAGCTA CAGTCTTGCG CCAAAGACGA GAAGTTGCCA AAACATTAGC
  541 TTTGTCGAGT AATGTGACGG GAGCAGGAGG GTTGGAAGTT TCAGCTAAGC GGCCGCGGGC
  601 CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG
  661 AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA
  721 ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG
  781 ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC
  841 CGAAATGTAA AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT
  901 AAAAAGTAGG GCAAGCGAGA CAGAATGGGA TACAAGGGCA TCGTCTATAA TTATAGCTAA
  961 AAATTGTATT TTAATTTGTA TTTTTTGTAA TTTTATTTCT CATTGTTTTA CTTAAAATGA
 1021 ATGCGAATTT AGAGAAAACT TTCACGAAAT GTCAAATAGC TCAGCAAAAT ATACCATTTT
 1081 AGGCAAGCGA TCAGCACTAT CTCGATGTAG ATCCTTTTCC TTCATAAATT TCCAACAACT
1141 GTA
//
Primer: URA4pro_F RDB14202_A6Fnd.seq
>06544_14202_A6Fn_o_2_URA4pro_F_D12_12.ab1
    1 TTGTATCGTA CGTATTGTCC TACCAAGAAC CTCTTTTTTG CTTGGATCGA AATTAAAGGT
   61 TTAAAAGCAA AGTTATGGAT GCTAGAGTAT TTCAAAGCTA TTCAGCTAGA GCTGAGGGGA
  121 TGAAAAATCC CATTGCCAAG GAATTGTTGG CTTTGATGGA AGAAAAGCAA AGCAACTTGT
  181 CAGTCGCGGT CGATTTGACG AAGAAATCCG AAATCTTAGA ATTGGTAGAT AAAATTGGAC
  241 CCTATGTCTG TGTTATCAAG ACACATATTG ACGTTGTCGA GGATTTCGAC CAGGATATGG
  301 TAGAAAAACT GGTGGCCTTA GGTAAAAAGC ATCGTTTTCT TATCTTTGAG GATCGCAAAT
  361 TCGCAGACAT TGGAAATACC GTCAAGCTAC AATATGCATC TGGTGTGTAC AAAATTGCTT
  421 CTTGGGCTCA TATCACAAAT TGCCATACAG TGCCAGGCGA GGGTATTATA CAAGGCCTCA
  481 AAGAAGTTGG TTTACCTTTG GGACGTGGTC TCTTGCTTTT GGCTGAAATG TCTTCCAAAG
  541 GCTCTTTGGC TACTGGTTCC TACACAGAGA AAACCTTAGA ATGGTTTGAG AAGCATACCG
  601 ATTTTTGCTT TGGCTTTATA GCTGGTCGTC GATTTCCTAA CCTTCAAAGC GACTACATAA
  661 CTATGTCCCC TGGTATCGGC TTGGATGTTA AAGGAGACGG GCTGGGACAG CAATATCGTA
  721 CTCCTGAAGA AGTGATTGTA AACTGCGGTA GCGACATCAT CATTGTTGGT CGTGGAGTCT
  781 ATGGAGCTGG TCGTAATCCT GTTGTCGAAG CCAAGAGATA TAGAGAAGCT GGTTGGAAGG
  841 CATATCAGCA AAGACTTTCT CAGCATTAAA AAAAGACTAA TGTAAAATTT TTTTTGGTTG
  901 GTTATTGAAA AAGTCGATGC CTTGTTTGCG TTTGTTTTCC TAGGCGTTTT ATGTCAGAAC
  961 GCATTTAGAA TTAGTATACA AGTACTCTTT GGGTAAAATT TTATGTAGCG ACTAAAATAT
 1021 TACTATTATA GATAAACACC TTTGGAATAA AAAGTTATTG CTATAGTTAT TTATAAACAT
 1081 GCTTCTTACA ACATAACACA TCGTTCTCAT GTATTGACAT TGAATAGAAA GAAGTGAGTT
1141 TTTTTAGAAC TG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl