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pDUAL-HFF61

S. pombe 5 ter end His6-FLAG2-tagged vector with ef1a-c promoter

Catalog number RDB06534
Resource name pDUAL-HFF61
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with ef1a-c promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364122 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06534 pDUAL-HFF61 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06534z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12779_A4Dp.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB12779_A4Dpa.seq check
            >06534_12779_A4Dp_ADHrev_H08_23.ab1
                1 TGAAGGGGTT CCTTCAAAGA ATAAGAATTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA
               61 TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA
              121 ATTCGCTTAT TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT
              181 GTCATCGTCG TCCTTGTAGT CCATGGGTTT ATCGTCATCA TCCTTATAAT CCATATGATG
              241 ATGGTGGTGA TGCATAGGAT CCAGGCCTGT CGACGCTAGC GTGAGTTCGA TGAAACAGAC
              301 TCTAGGTAGA AGCGAAGGGC GCCAGCGCCT ACATATATAC TTGCGCAAAA AAAGCAGTCA
              361 CATAACGTTC GTTACGTCTC GAAAGTTTTT TTCTTTTTTT CCGGCTTAAA ATATTGTCGT
              421 GAGCCTATCA GCGAACGAAT TTTGTTTGCG CAGGAAAGTC ATGATGTCAT TTAAAATTTT
              481 TCTTTGCTTC TTAACCTTTT GTCTTTAGAG TACACTTAGC CGAATTTGCC ACCGCAATAA
              541 ACATTAGCTT AGCGAACGTC GTTCTTGGCG ATTTTGATTT ATTGGGCGGA AACGATCAAC
              601 GCTGTATATA TGAAATGGAT CGTTTTTCGT TAATTTAAAA ATATATATCT GGTTTATTAA
              661 ATGATGAAAA ATATTTAGTT GTATCATTGA ACCATTTTTG ATGGCCGTTA TCAGATCGGA
              721 TTGAGATTGT GGACTGAGAT TTTCGCTGAT ATACCCACCT TAGCTGCTTT TGTTTGTTCT
              781 TTGTCCGTCA CCACAACGGT AATAGTTTTG ATGGCTTTGC TTTTGGAGGG TACTTCTTTG
              841 GCCCGGTATG TCTTTTTTAT CGTGTATATA TAGTTTTGTC TAACAGTTGA ATTAGCAAAG
              901 TTCGAGAAGA ACTTCGGGAG CAGATCTCCT CGATTAAATC TGTTGACCCT TATTTCAACG
              961 TTTCTTTAAA AATCATTCAA GTCGGAGGTA GAGAGGACTC AAATGTATAT GTGCGCATGA
             1021 AAACTCGTGC AGCTAATGAG GTATGAGTGA AGGCGAGAAG GTCTCCTAAA TGATTGAAAT
             1081 GCATGCAAGC TTGGCGTAAT CATGTTCATA GCTGTTTCCT GTGTTGAATT GTTATCCGCT
             1141 CCACATCCAC ACAACAATAC GAGTCGGAGC CATAAAGTGT AAAGGCCTGG GGGGTGCCTA
            1201 TTGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

            2022.05.18

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