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pDUAL-HFF61

S. pombe 5 ter end His6-FLAG2-tagged vector with ef1a-c promoter

Catalog number RDB06534
Resource name pDUAL-HFF61
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with ef1a-c promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364122 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06534 pDUAL-HFF61 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06534z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12779_A4Dp.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev RDB12779_A4Dpa.seq
>06534_12779_A4Dp_ADHrev_H08_23.ab1
    1 TGAAGGGGTT CCTTCAAAGA ATAAGAATTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA
   61 TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA
  121 ATTCGCTTAT TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT
  181 GTCATCGTCG TCCTTGTAGT CCATGGGTTT ATCGTCATCA TCCTTATAAT CCATATGATG
  241 ATGGTGGTGA TGCATAGGAT CCAGGCCTGT CGACGCTAGC GTGAGTTCGA TGAAACAGAC
  301 TCTAGGTAGA AGCGAAGGGC GCCAGCGCCT ACATATATAC TTGCGCAAAA AAAGCAGTCA
  361 CATAACGTTC GTTACGTCTC GAAAGTTTTT TTCTTTTTTT CCGGCTTAAA ATATTGTCGT
  421 GAGCCTATCA GCGAACGAAT TTTGTTTGCG CAGGAAAGTC ATGATGTCAT TTAAAATTTT
  481 TCTTTGCTTC TTAACCTTTT GTCTTTAGAG TACACTTAGC CGAATTTGCC ACCGCAATAA
  541 ACATTAGCTT AGCGAACGTC GTTCTTGGCG ATTTTGATTT ATTGGGCGGA AACGATCAAC
  601 GCTGTATATA TGAAATGGAT CGTTTTTCGT TAATTTAAAA ATATATATCT GGTTTATTAA
  661 ATGATGAAAA ATATTTAGTT GTATCATTGA ACCATTTTTG ATGGCCGTTA TCAGATCGGA
  721 TTGAGATTGT GGACTGAGAT TTTCGCTGAT ATACCCACCT TAGCTGCTTT TGTTTGTTCT
  781 TTGTCCGTCA CCACAACGGT AATAGTTTTG ATGGCTTTGC TTTTGGAGGG TACTTCTTTG
  841 GCCCGGTATG TCTTTTTTAT CGTGTATATA TAGTTTTGTC TAACAGTTGA ATTAGCAAAG
  901 TTCGAGAAGA ACTTCGGGAG CAGATCTCCT CGATTAAATC TGTTGACCCT TATTTCAACG
  961 TTTCTTTAAA AATCATTCAA GTCGGAGGTA GAGAGGACTC AAATGTATAT GTGCGCATGA
 1021 AAACTCGTGC AGCTAATGAG GTATGAGTGA AGGCGAGAAG GTCTCCTAAA TGATTGAAAT
 1081 GCATGCAAGC TTGGCGTAAT CATGTTCATA GCTGTTTCCT GTGTTGAATT GTTATCCGCT
 1141 CCACATCCAC ACAACAATAC GAGTCGGAGC CATAAAGTGT AAAGGCCTGG GGGGTGCCTA
1201 TTGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702.

2018.12.10

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