Resource data sheet
Prev.

pHFG1-ccdB2

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number RDB06525
Resource name pHFG1-ccdB2
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter.
Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06525 pHFG1-ccdB2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06525z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12297_A7Fep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12297_A7Fea.seq
>06512_12297_A7Fe_nmt1Fw_A11_01_ABI08.ab1
    1 AGGAAATTTA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
  241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
  301 ATCCTATGCA TCACCACCAT CATCATATGG ATTATAAGGA TGATGACGAT AAACCCATGG
  361 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  421 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  481 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  541 TGACCACCCT GACCTGGGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  601 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  661 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  721 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  781 TGGAGTACAA CTACATCAGC CACAACGTCT ATATCACCGC CGACAAGCAG AAGAACGGCA
  841 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  901 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  961 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
 1021 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGATATCA
 1081 CAGTTTGTAC AAAAAAAAGC CTGAACGAGA AACCGTAAAT GATATAAATA TCATATATTT
 1141 AAAATAGATT TTGCATAAAA ACAGACTACC TAATACCTGG TAACACCACT ATATCCCAGG
1201 TCCACCTTAT TGGGCGGCGG C
//
Primer: Cm_M_R | Sequence: RDB12297_A7Feb.seq
>06512_12297_A7Fe_Cm_M_R_B11_02_ABI08.ab1
    1 GTCTAGGGGT GACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAATT
   61 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT
  121 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG
  181 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT
  241 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC
  301 TCGACGGATC CTAACTCAAA ATCCACACAT TATACGAGCC GGAAGCATAA AGTGTAAAGC
  361 CTGGGGTGCC TAATGCGGCC GCCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT
  421 AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC
  481 TCGTTCAGCT TTTTTGTACA AACTTGTGAT ATCCTTGTAC AGCTCGTCCA TGCCGAGAGT
  541 GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC
  601 TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC
  661 GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCGATGTT
  721 GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC
  781 GTTGTGGCTG ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA
  841 GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGGTGTC GCCCTCGAAC TTCACCTCGG
  901 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT
  961 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC
 1021 ACTGCACGCC CCAGGTCAGG GTGGGTCACG GAGGGTGGGG CCAGGCACGG GCAGCTTGCT
 1081 GGTGGGTGCA GATGAACTTC AAGGTCAGCT TTGCGGTAGG TGGCATCGGC CCTCGTCCCT
 1141 CGCCGGACAC GCTGAAACCT GTGGCCGGTT AACGGTTCGC GTCCAGCCTC GGAACCAGGA
1201 ATGGCACCAA CCCCGGGGTG T
//
Primer: nmt1ter_R | Sequence: RDB12297_A7Fec.seq
>06512_12297_A7Fe_nmt1ter_R_C11_03_ABI08.ab1
    1 GAACGTCATA GGTTACTCAT GTATAAACGT TTTGGGTTCA TATTTTTGGG CCACGTGGTT
   61 TATAAAAATT CTTAACTACA CCACTCGGTA TCCCGCACCC GTCTACGTTT CTACGATTTC
  121 GAGCTAATAT TGAGTAAAGT GATTAGCAAA AAAAATAAAA GTACTCGTTG TCGGAGATCA
  181 AGAATTTTTC TATTATCTCA TCTAAACCAC TTTCTAAAAG CGAAAAACAA AATCGTAATA
  241 TGCAGCTTGA ATGGGCTTCC ATAGTTTGAA AGAAAAACCC TAGCAGTACT GGCAAGGGAG
  301 ACATTCCTTT TACCCTTAGT TAGTTAGCGG CCGCCCGGGC TCGAGATCTC ATGATATCAC
  361 CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA ATATATTAAA
  421 TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA TCCAGTCACT
  481 ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT CCCCAGAACA
  541 TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC ACTTCTTCCC
  601 CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG CTTTCATCCC
  661 CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA CGTGCACTGG
  721 CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT ACATCCACAA
  781 ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT TCACCAGCCC
  841 CTGTTCTCGT CAGCAAAAGA GCCGTTCATT TCAATAAACC GGGCGACCTC AGCCATCCCT
  901 TCCTGATTTT CCGCTTTCCA GCGTTCGGCA CGCAGACGAC GGGGCTTCAT TTCTGCATGG
  961 TTGTGCTTAC CAGACCGGAG ATATTGACAT CATATATGCC TTGAGCAACT GATAGCTGTC
 1021 GCTGTCAACT GTCACTGTAA TAACGCTGCT TCATAGCATA CCTCTTTTTG ACATACTTTC
 1081 GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAAAT CAGCGGCGGC AAAATTACGC
 1141 AATACTGGTT ATTCTGGCTT TTAGTTAAGG CCGATTCCCA CGCGATTTAC CGCCCGGCTG
1201 CAACTCATCG CAAGTTAACT GGTGTTGATA ATCTTAC
//
Primer: f1ori_F | Sequence: RDB12297_A7Fed.seq
>06512_12297_A7Fe_f1ori_F_D11_04_ABI08.ab1
    1 GCGGGCGCTA AGCGCTGGCA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC
   61 GCTTAATGCG CCGCTACAGG GCGCGTACTA TGGTTGCTTT GACGTATGCG GTGTGAAATA
  121 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC
  181 AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG
  241 GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT
  301 AAAACGACGG CCAGTGAATT CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG
  361 ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT
  421 TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA
  481 ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT
  541 TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG CGGCAGCGTT
  601 CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG ATACAAGGGC
  661 ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTGTA ATTTTATTTC
  721 TCATTGTTTT ACTTAAAATG AATGCGAATT AGAGAAAACT TCAACGAAAT GTCAAAATAA
  781 GCTCAGCAAA ATATACAATT TTAGGGAAAG CGATCAGCAA CTTATTCTCC GTTGTAGATT
  841 CTTTTTCTTC ATAAATTCCA ACAATGTAAC TGCCAATTGA TTTTTCTCTA GATGCTAATA
  901 AATTGAAACT AATGAATATA ATAACACAGG TTTTGATAAA TTGTAAACTC TTACTCAAAA
  961 AAAGAATGTT AAAACTAATT TAACCCTTTT TATAAAGGCT CATGGCGTTT AAAGAGGATA
 1021 GTAAAAATTC TACAATTTTT TAATTTAATG TCACAATTGT CTTTGCGATA TATTCTATTA
 1081 AATTTTCGCT AACTGCTGTT TAGTACTATA CTTTCATATG CCTTTATTGC ACCTTAGTCC
1141 ATGCAGAATG CATATTTTTG CAATCTTTTC TTTGTTAACC TGAGAAGGCG GC
//
Primer: LEU2pro_F | Sequence: RDB12297_A7Fee.seq
>06512_12297_A7Fe_LEU2pro_F_E11_05_ABI08.ab1
    1 CAAACGTTGC AGTTGACATA TTATTTAAGG ACCTATTGTT TTTTCCAATA GGTGGTTAGC
   61 AATCGTCTTA CTTTCTAACT TTTCTTACCT TTTACATTTC AGCAATATAT ATATATATTT
  121 CAAGGATATA CCATTCTAAT GTCTGCCCCT AAGAAGATCG TCGTTTTGCC AGGTGACCAC
  181 GTTGGTCAAG AAATCACAGC CGAAGCCATT AAGGTTCTTA AAGCTATTTC TGATGTTCGT
  241 TCCAATGTCA AGTTCGATTT CGAAAATCAT TTAATTGGTG GTGCTGCTAT CGATGCTACA
  301 GGTGTTCCAC TTCCAGATGA GGCGCTGGAA GCCTCCAAGA AGGCTGATGC CGTTTTGTTA
  361 GGTGCTGTGG GTGGTCCTAA ATGGGGTACC GGTAGTGTTA GACCTGAACA AGGTTTACTA
  421 AAAATCCGTA AAGAACTTCA ATTGTACGCC AACTTAAGAC CATGTAACTT TGCATCCGAC
  481 TCTCTTTTAG ACTTATCTCC AATCAAGCCA CAATTTGCTA AAGGTACTGA CTTCGTTGTT
541 GTCAGAGAAT TAGTGGGAGG TATTTACTTT GG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

GNP_filter3_RDBDEP_html_190927.pl