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pHFG1-ccdB2

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number RDB06525
Resource name pHFG1-ccdB2
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06525 pHFG1-ccdB2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06525z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12297_A7Fep1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB12297_A7Fea.seq check
            >06512_12297_A7Fe_nmt1Fw_A11_01_ABI08.ab1
                1 AGGAAATTTA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
              241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
              301 ATCCTATGCA TCACCACCAT CATCATATGG ATTATAAGGA TGATGACGAT AAACCCATGG
              361 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
              421 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
              481 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
              541 TGACCACCCT GACCTGGGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
              601 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
              661 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
              721 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
              781 TGGAGTACAA CTACATCAGC CACAACGTCT ATATCACCGC CGACAAGCAG AAGAACGGCA
              841 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
              901 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
              961 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
             1021 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGATATCA
             1081 CAGTTTGTAC AAAAAAAAGC CTGAACGAGA AACCGTAAAT GATATAAATA TCATATATTT
             1141 AAAATAGATT TTGCATAAAA ACAGACTACC TAATACCTGG TAACACCACT ATATCCCAGG
            1201 TCCACCTTAT TGGGCGGCGG C
            //
            Primer: Cm_M_R
            Sequence file: RDB12297_A7Feb.seq check
            >06512_12297_A7Fe_Cm_M_R_B11_02_ABI08.ab1
                1 GTCTAGGGGT GACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAATT
               61 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT
              121 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG
              181 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT
              241 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC
              301 TCGACGGATC CTAACTCAAA ATCCACACAT TATACGAGCC GGAAGCATAA AGTGTAAAGC
              361 CTGGGGTGCC TAATGCGGCC GCCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT
              421 AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC
              481 TCGTTCAGCT TTTTTGTACA AACTTGTGAT ATCCTTGTAC AGCTCGTCCA TGCCGAGAGT
              541 GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC
              601 TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC
              661 GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCGATGTT
              721 GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC
              781 GTTGTGGCTG ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA
              841 GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGGTGTC GCCCTCGAAC TTCACCTCGG
              901 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT
              961 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC
             1021 ACTGCACGCC CCAGGTCAGG GTGGGTCACG GAGGGTGGGG CCAGGCACGG GCAGCTTGCT
             1081 GGTGGGTGCA GATGAACTTC AAGGTCAGCT TTGCGGTAGG TGGCATCGGC CCTCGTCCCT
             1141 CGCCGGACAC GCTGAAACCT GTGGCCGGTT AACGGTTCGC GTCCAGCCTC GGAACCAGGA
            1201 ATGGCACCAA CCCCGGGGTG T
            //
            Primer: nmt1ter_R
            Sequence file: RDB12297_A7Fec.seq check
            >06512_12297_A7Fe_nmt1ter_R_C11_03_ABI08.ab1
                1 GAACGTCATA GGTTACTCAT GTATAAACGT TTTGGGTTCA TATTTTTGGG CCACGTGGTT
               61 TATAAAAATT CTTAACTACA CCACTCGGTA TCCCGCACCC GTCTACGTTT CTACGATTTC
              121 GAGCTAATAT TGAGTAAAGT GATTAGCAAA AAAAATAAAA GTACTCGTTG TCGGAGATCA
              181 AGAATTTTTC TATTATCTCA TCTAAACCAC TTTCTAAAAG CGAAAAACAA AATCGTAATA
              241 TGCAGCTTGA ATGGGCTTCC ATAGTTTGAA AGAAAAACCC TAGCAGTACT GGCAAGGGAG
              301 ACATTCCTTT TACCCTTAGT TAGTTAGCGG CCGCCCGGGC TCGAGATCTC ATGATATCAC
              361 CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA ATATATTAAA
              421 TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA TCCAGTCACT
              481 ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT CCCCAGAACA
              541 TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC ACTTCTTCCC
              601 CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG CTTTCATCCC
              661 CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA CGTGCACTGG
              721 CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT ACATCCACAA
              781 ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT TCACCAGCCC
              841 CTGTTCTCGT CAGCAAAAGA GCCGTTCATT TCAATAAACC GGGCGACCTC AGCCATCCCT
              901 TCCTGATTTT CCGCTTTCCA GCGTTCGGCA CGCAGACGAC GGGGCTTCAT TTCTGCATGG
              961 TTGTGCTTAC CAGACCGGAG ATATTGACAT CATATATGCC TTGAGCAACT GATAGCTGTC
             1021 GCTGTCAACT GTCACTGTAA TAACGCTGCT TCATAGCATA CCTCTTTTTG ACATACTTTC
             1081 GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAAAT CAGCGGCGGC AAAATTACGC
             1141 AATACTGGTT ATTCTGGCTT TTAGTTAAGG CCGATTCCCA CGCGATTTAC CGCCCGGCTG
            1201 CAACTCATCG CAAGTTAACT GGTGTTGATA ATCTTAC
            //
            Primer: f1ori_F
            Sequence file: RDB12297_A7Fed.seq check
            >06512_12297_A7Fe_f1ori_F_D11_04_ABI08.ab1
                1 GCGGGCGCTA AGCGCTGGCA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC
               61 GCTTAATGCG CCGCTACAGG GCGCGTACTA TGGTTGCTTT GACGTATGCG GTGTGAAATA
              121 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC
              181 AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG
              241 GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT
              301 AAAACGACGG CCAGTGAATT CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG
              361 ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT
              421 TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA
              481 ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT
              541 TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG CGGCAGCGTT
              601 CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG ATACAAGGGC
              661 ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTGTA ATTTTATTTC
              721 TCATTGTTTT ACTTAAAATG AATGCGAATT AGAGAAAACT TCAACGAAAT GTCAAAATAA
              781 GCTCAGCAAA ATATACAATT TTAGGGAAAG CGATCAGCAA CTTATTCTCC GTTGTAGATT
              841 CTTTTTCTTC ATAAATTCCA ACAATGTAAC TGCCAATTGA TTTTTCTCTA GATGCTAATA
              901 AATTGAAACT AATGAATATA ATAACACAGG TTTTGATAAA TTGTAAACTC TTACTCAAAA
              961 AAAGAATGTT AAAACTAATT TAACCCTTTT TATAAAGGCT CATGGCGTTT AAAGAGGATA
             1021 GTAAAAATTC TACAATTTTT TAATTTAATG TCACAATTGT CTTTGCGATA TATTCTATTA
             1081 AATTTTCGCT AACTGCTGTT TAGTACTATA CTTTCATATG CCTTTATTGC ACCTTAGTCC
            1141 ATGCAGAATG CATATTTTTG CAATCTTTTC TTTGTTAACC TGAGAAGGCG GC
            //
            Primer: LEU2pro_F
            Sequence file: RDB12297_A7Fee.seq check
            >06512_12297_A7Fe_LEU2pro_F_E11_05_ABI08.ab1
                1 CAAACGTTGC AGTTGACATA TTATTTAAGG ACCTATTGTT TTTTCCAATA GGTGGTTAGC
               61 AATCGTCTTA CTTTCTAACT TTTCTTACCT TTTACATTTC AGCAATATAT ATATATATTT
              121 CAAGGATATA CCATTCTAAT GTCTGCCCCT AAGAAGATCG TCGTTTTGCC AGGTGACCAC
              181 GTTGGTCAAG AAATCACAGC CGAAGCCATT AAGGTTCTTA AAGCTATTTC TGATGTTCGT
              241 TCCAATGTCA AGTTCGATTT CGAAAATCAT TTAATTGGTG GTGCTGCTAT CGATGCTACA
              301 GGTGTTCCAC TTCCAGATGA GGCGCTGGAA GCCTCCAAGA AGGCTGATGC CGTTTTGTTA
              361 GGTGCTGTGG GTGGTCCTAA ATGGGGTACC GGTAGTGTTA GACCTGAACA AGGTTTACTA
              421 AAAATCCGTA AAGAACTTCA ATTGTACGCC AACTTAAGAC CATGTAACTT TGCATCCGAC
              481 TCTCTTTTAG ACTTATCTCC AATCAAGCCA CAATTTGCTA AAGGTACTGA CTTCGTTGTT
            541 GTCAGAGAAT TAGTGGGAGG TATTTACTTT GG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl