Please review the QC test results indicated by icon below as well as clone information before placing your order.

pHFG1-ccdB2 (#RDB06525)

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Clone info.	S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter.
Comment	Gateway compatible.
Vector backbone	pREP1 (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer	Yoshida, Minoru | Matsuyama, Akihisa |

 

Sequence	RDB06525z.seq

Distribution information

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06525	pHFG1-ccdB2	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pHFG1-ccdB2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06525).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12297_A7Fep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB12297_A7Fea.seq
>06512_12297_A7Fe_nmt1Fw_A11_01_ABI08.ab1     1 AGGAAATTTA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA    61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC   121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT   181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA   241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG   301 ATCCTATGCA TCACCACCAT CATCATATGG ATTATAAGGA TGATGACGAT AAACCCATGG   361 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG   421 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA   481 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG   541 TGACCACCCT GACCTGGGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC   601 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA   661 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA   721 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC   781 TGGAGTACAA CTACATCAGC CACAACGTCT ATATCACCGC CGACAAGCAG AAGAACGGCA   841 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC   901 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC   961 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC  1021 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGATATCA  1081 CAGTTTGTAC AAAAAAAAGC CTGAACGAGA AACCGTAAAT GATATAAATA TCATATATTT  1141 AAAATAGATT TTGCATAAAA ACAGACTACC TAATACCTGG TAACACCACT ATATCCCAGG 1201 TCCACCTTAT TGGGCGGCGG C //
Primer: Cm_M_R Sequence file: RDB12297_A7Feb.seq
>06512_12297_A7Fe_Cm_M_R_B11_02_ABI08.ab1     1 GTCTAGGGGT GACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAATT    61 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT   121 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG   181 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT   241 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC   301 TCGACGGATC CTAACTCAAA ATCCACACAT TATACGAGCC GGAAGCATAA AGTGTAAAGC   361 CTGGGGTGCC TAATGCGGCC GCCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT   421 AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC   481 TCGTTCAGCT TTTTTGTACA AACTTGTGAT ATCCTTGTAC AGCTCGTCCA TGCCGAGAGT   541 GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC   601 TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC   661 GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCGATGTT   721 GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC   781 GTTGTGGCTG ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA   841 GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGGTGTC GCCCTCGAAC TTCACCTCGG   901 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT   961 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC  1021 ACTGCACGCC CCAGGTCAGG GTGGGTCACG GAGGGTGGGG CCAGGCACGG GCAGCTTGCT  1081 GGTGGGTGCA GATGAACTTC AAGGTCAGCT TTGCGGTAGG TGGCATCGGC CCTCGTCCCT  1141 CGCCGGACAC GCTGAAACCT GTGGCCGGTT AACGGTTCGC GTCCAGCCTC GGAACCAGGA 1201 ATGGCACCAA CCCCGGGGTG T //
Primer: nmt1ter_R Sequence file: RDB12297_A7Fec.seq
>06512_12297_A7Fe_nmt1ter_R_C11_03_ABI08.ab1     1 GAACGTCATA GGTTACTCAT GTATAAACGT TTTGGGTTCA TATTTTTGGG CCACGTGGTT    61 TATAAAAATT CTTAACTACA CCACTCGGTA TCCCGCACCC GTCTACGTTT CTACGATTTC   121 GAGCTAATAT TGAGTAAAGT GATTAGCAAA AAAAATAAAA GTACTCGTTG TCGGAGATCA   181 AGAATTTTTC TATTATCTCA TCTAAACCAC TTTCTAAAAG CGAAAAACAA AATCGTAATA   241 TGCAGCTTGA ATGGGCTTCC ATAGTTTGAA AGAAAAACCC TAGCAGTACT GGCAAGGGAG   301 ACATTCCTTT TACCCTTAGT TAGTTAGCGG CCGCCCGGGC TCGAGATCTC ATGATATCAC   361 CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA ATATATTAAA   421 TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA TCCAGTCACT   481 ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT CCCCAGAACA   541 TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC ACTTCTTCCC   601 CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG CTTTCATCCC   661 CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA CGTGCACTGG   721 CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT ACATCCACAA   781 ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT TCACCAGCCC   841 CTGTTCTCGT CAGCAAAAGA GCCGTTCATT TCAATAAACC GGGCGACCTC AGCCATCCCT   901 TCCTGATTTT CCGCTTTCCA GCGTTCGGCA CGCAGACGAC GGGGCTTCAT TTCTGCATGG   961 TTGTGCTTAC CAGACCGGAG ATATTGACAT CATATATGCC TTGAGCAACT GATAGCTGTC  1021 GCTGTCAACT GTCACTGTAA TAACGCTGCT TCATAGCATA CCTCTTTTTG ACATACTTTC  1081 GGGTATACAT ATCAGTATAT ATTCTTATAC CGCAAAAAAT CAGCGGCGGC AAAATTACGC  1141 AATACTGGTT ATTCTGGCTT TTAGTTAAGG CCGATTCCCA CGCGATTTAC CGCCCGGCTG 1201 CAACTCATCG CAAGTTAACT GGTGTTGATA ATCTTAC //
Primer: f1ori_F Sequence file: RDB12297_A7Fed.seq
>06512_12297_A7Fe_f1ori_F_D11_04_ABI08.ab1     1 GCGGGCGCTA AGCGCTGGCA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC    61 GCTTAATGCG CCGCTACAGG GCGCGTACTA TGGTTGCTTT GACGTATGCG GTGTGAAATA   121 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC   181 AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG   241 GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT   301 AAAACGACGG CCAGTGAATT CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG   361 ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT   421 TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA   481 ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT   541 TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG CGGCAGCGTT   601 CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG ATACAAGGGC   661 ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTGTA ATTTTATTTC   721 TCATTGTTTT ACTTAAAATG AATGCGAATT AGAGAAAACT TCAACGAAAT GTCAAAATAA   781 GCTCAGCAAA ATATACAATT TTAGGGAAAG CGATCAGCAA CTTATTCTCC GTTGTAGATT   841 CTTTTTCTTC ATAAATTCCA ACAATGTAAC TGCCAATTGA TTTTTCTCTA GATGCTAATA   901 AATTGAAACT AATGAATATA ATAACACAGG TTTTGATAAA TTGTAAACTC TTACTCAAAA   961 AAAGAATGTT AAAACTAATT TAACCCTTTT TATAAAGGCT CATGGCGTTT AAAGAGGATA  1021 GTAAAAATTC TACAATTTTT TAATTTAATG TCACAATTGT CTTTGCGATA TATTCTATTA  1081 AATTTTCGCT AACTGCTGTT TAGTACTATA CTTTCATATG CCTTTATTGC ACCTTAGTCC 1141 ATGCAGAATG CATATTTTTG CAATCTTTTC TTTGTTAACC TGAGAAGGCG GC //
Primer: LEU2pro_F Sequence file: RDB12297_A7Fee.seq
>06512_12297_A7Fe_LEU2pro_F_E11_05_ABI08.ab1     1 CAAACGTTGC AGTTGACATA TTATTTAAGG ACCTATTGTT TTTTCCAATA GGTGGTTAGC    61 AATCGTCTTA CTTTCTAACT TTTCTTACCT TTTACATTTC AGCAATATAT ATATATATTT   121 CAAGGATATA CCATTCTAAT GTCTGCCCCT AAGAAGATCG TCGTTTTGCC AGGTGACCAC   181 GTTGGTCAAG AAATCACAGC CGAAGCCATT AAGGTTCTTA AAGCTATTTC TGATGTTCGT   241 TCCAATGTCA AGTTCGATTT CGAAAATCAT TTAATTGGTG GTGCTGCTAT CGATGCTACA   301 GGTGTTCCAC TTCCAGATGA GGCGCTGGAA GCCTCCAAGA AGGCTGATGC CGTTTTGTTA   361 GGTGCTGTGG GTGGTCCTAA ATGGGGTACC GGTAGTGTTA GACCTGAACA AGGTTTACTA   421 AAAATCCGTA AAGAACTTCA ATTGTACGCC AACTTAAGAC CATGTAACTT TGCATCCGAC   481 TCTCTTTTAG ACTTATCTCC AATCAAGCCA CAATTTGCTA AAGGTACTGA CTTCGTTGTT 541 GTCAGAGAAT TAGTGGGAGG TATTTACTTT GG //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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