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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHFF1-ccdB2 (#RDB06522)

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06522z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06522 pHFF1-ccdB2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHFF1-ccdB2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06522).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12286_A3Jm.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB12286_A3Jma.seq check
>06522_12286_A3Jm_nmt1Fw_C05_08.ab1
    1 TAAATTTAAT TTAATCCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
  241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
  301 GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG
  361 GACTACAAGG ACGACGATGA CAAAGATATC ACAAGTTTGT ACAAAAAAGC TGAACGAGAA
  421 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA
  481 TAATACTGTA AAACACAACA TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT
  541 TTACACTTTA TGCTTCCGGC TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT
  601 TTTCAGGAGC TAAGGAAGCT AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA
  661 TATCCCAATG GCATCGTAAA GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT
  721 ATAACCAGAC CGTTCAGCTG GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC
  781 ACAAGTTTTA TCCGGCCTTT ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT
  841 TCCGTATGGC AATGAAAGAC GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA
  901 CCGTTTTCCA TGAGCAAACT GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT
  961 TCCGGCAGTT TCTACACATA TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCCTGGCC
 1021 TATTTCCCTA AAAGGGTTTA TTTGAGAATA TGTTTTTTCG TCTCAGCCCA AATCCCTGGG
 1081 TGAGTTTCAC CAAGTTTTTG GAATTAACCG TGACAATATG GACACTTTCT TCGTCCCCCG
1141 AATTTTTCAA
//
Primer: nmt1Rv
Sequence file: RDB12286_A3Jmb.seq check
>06522_12286_A3Jm_nmt1Rv_D05_11.ab1
    1 AGCCGCCCGG GGCCTCGAGA TCTCATGATA TCACCACTTT GTACAAGAAA GCTGAACGAG
   61 AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA
  121 CATAATACTG TAAAACACAA CATATCCAGT CACTATGGTC GACCTGCAGA CTGGCTGTGT
  181 ATAAGGGAGC CTGACATTTA TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA
  241 TTTTCGCGGT GGCTGAGATC AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC
  301 ATATCGGTGG TCATCATGCG CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA
  361 CGGGAGACTT TATCTGACAG CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG
  421 GGCGTGTCAA TAATATCACT CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA
  481 TAGGTGTAAA CCTTAAACTG CATTTCACCA GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT
  541 CATTTCAATA AACCGGGCGA CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC
  601 GGCACGCAGA CGACGGGCTT CATTCTGCAT GGTTGTGCTT ACCAGACCGG AGATATTGAC
  661 ATCATATATG CCTTGAGCAA CTGATAGCTG TCGCTGTCAA CTGTCACTGT AATACGCTGC
  721 TTCATAGCAT ACCTCTTTTT GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC
  781 CGCAAAAATC AGCGCGCAAA TACGCATACT GTTATCTGGC TTTTAGTAAG CCGGATCCAC
  841 GCGTTTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC
  901 CGACATGGAA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC
  961 TTGTCGCCTT GCGTATAATA TTTGCCCATG GGTGAAAACG GGGGCGAAGA AGTTGTCCAT
 1021 ATTGGTCCAC GTTTAAATCA AAACTGGTGA ACTCACCCAG GGATTGGCTG AGACGAAAAA
1081 CATATTCTCA TAATCCTTCG GAATAGGCCA GGTTTTCACC CGTAAACCAC GGCCCCAAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles