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pREP81-ccdB2

S. pombe expression vector with nmt1 (low strength) promoter

Catalog number RDB06521
Resource name pREP81-ccdB2
Clone info. S. pombe expression vector with nmt1 (low strength) promoter
Gateway compatible.
Vector backbone pREP81 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06521 pREP81-ccdB2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06521z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12285_A3Jm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12285_A3Jma.seq
>06521_12285_A3Jm_nmt1Fw_A05_02.ab1
    1 TGGATATTTT ATTTAATTCA GGGAAAAACG TAACTCTCGG CTACTGGATG GTTCAGTCAC
   61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC
  121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA
  181 AGATGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC
  241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC
  301 CGGATATCAA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC
  361 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT
  421 ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT
  481 CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA
  541 AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG
  601 AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG
661 ATATTACGGC CTTTTTA
//
Primer: nmt1Rv | Sequence: RDB12285_A3Jmb.seq
>06521_12285_A3Jm_nmt1Rv_B05_05.ab1
    1 AGCCGCCGGG GGCTGGAGAT CTCATGATAT CACCACTTTG TACAAGAAAG CTGAACGAGA
   61 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC
  121 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGTCG ACCTGCAGAC TGGCTGTGTA
  181 TAAGGGAGCC TGACATTTAT ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT
  241 TTTCGCGGTG GCTGAGATCA GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA
  301 TATCGGTGGT CATCATGCGC CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC
  361 GGGAGACTTT ATCTGACAGC AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG
  421 GCGTGTCAAT AATATCACTC TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT
  481 AGGTGTAAAC CTTAAACTGC ATTTCACCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC
  541 ATTTCAATAA ACCGGGCGAC CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG
  601 GCACGCAGAC GACGGGCTTC ATTCTGCATG GTTGTGCTTA CCAGACCGGA GATATTGACA
661 TCATATATGC CTTGAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

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