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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pREP81-ccdB2

S. pombe expression vector with nmt1 (low strength) promoter

Catalog number RDB06521
Resource name pREP81-ccdB2
Clone info. S. pombe expression vector with nmt1 (low strength) promoter
Comment Gateway compatible.
Vector backbone pREP81 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

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            RDB06521 pREP81-ccdB2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06521z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12285_A3Jm.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB12285_A3Jma.seq check
            >06521_12285_A3Jm_nmt1Fw_A05_02.ab1
                1 TGGATATTTT ATTTAATTCA GGGAAAAACG TAACTCTCGG CTACTGGATG GTTCAGTCAC
               61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC
              121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA
              181 AGATGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC
              241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC
              301 CGGATATCAA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC
              361 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT
              421 ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT
              481 CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT AAGGAAGCTA
              541 AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG
              601 AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG
            661 ATATTACGGC CTTTTTA
            //
            Primer: nmt1Rv
            Sequence file: RDB12285_A3Jmb.seq check
            >06521_12285_A3Jm_nmt1Rv_B05_05.ab1
                1 AGCCGCCGGG GGCTGGAGAT CTCATGATAT CACCACTTTG TACAAGAAAG CTGAACGAGA
               61 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC
              121 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGTCG ACCTGCAGAC TGGCTGTGTA
              181 TAAGGGAGCC TGACATTTAT ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT
              241 TTTCGCGGTG GCTGAGATCA GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA
              301 TATCGGTGGT CATCATGCGC CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC
              361 GGGAGACTTT ATCTGACAGC AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG
              421 GCGTGTCAAT AATATCACTC TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT
              481 AGGTGTAAAC CTTAAACTGC ATTTCACCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC
              541 ATTTCAATAA ACCGGGCGAC CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG
              601 GCACGCAGAC GACGGGCTTC ATTCTGCATG GTTGTGCTTA CCAGACCGGA GATATTGACA
            661 TCATATATGC CTTGAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.05.18

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