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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pREP1-ccdB2

S. pombe vector with nmt1 promoter

Catalog number RDB06519
Resource name pREP1-ccdB2
Clone info. S. pombe vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

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            RDB06519 pREP1-ccdB2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06519z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0025j (Japanese text)
            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12283_A3Jmp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB12283_A3Jma_v2.seq check
            >06519_12283_A3Jm_nmt1Fw_E04_13.ab1
                1 GAAATTTATT TAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
               61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
              121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
              181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
              241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
              301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT
              361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA
              421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
              481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT
              541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA
              601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG
              661 GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT
              721 ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC
              781 GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT
              841 GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT TCTACACATA
              901 TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCTGGCCT ATTTCCCTAA AGGGTTTATT
              961 GAGAATATGT TTTTTCGTCT CAGCCCAATC CCTGGTGAGT TTCACCAGTT TTGATTTAAA
             1021 CGTGGCCAAT ATGGACAACT TCTTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC
             1081 AAGGCGACAG GTTGCCTGAA TGCGCTGACC GAATTCAGAT TCATCATGCC CGGATTTGGT
            1141 TGAATGGGCC T
            //
            Primer: nmt1Rv
            Sequence file: RDB12283_A3Jmb_v2.seq check
            >06519_12283_A3Jm_nmt1Rv_F04_16.ab1
                1 GGGGCGCCCG GGGCCTGGAG ATCTCATGAT ATCACCACTT TGTACAAGAA AGCTGAACGA
               61 GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT
              121 ACATAATACT GTAAAACACA ACATATCCAG TCACTATGGT CGACCTGCAG ACTGGCTGTG
              181 TATAAGGGAG CCTGACATTT ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC
              241 ATTTTCGCGG TGGCTGAGAT CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC
              301 CATATCGGTG GTCATCATGC GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC
              361 ACGGGAGACT TTATCTGACA GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC
              421 GGGCGTGTCA ATAATATCAC TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT
              481 ATAGGTGTAA ACCTTAAACT GCATTTCACC AGCCCCTGTT CTCGTCAGCA AAAGAGCCGT
              541 TCATTTCAAT AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT
              601 CGGCACGCAG ACGACGGGCT TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA
              661 CATCATATAT GCCTTGAGCA ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG
              721 CTTCATAGCA TACCTCTTTT TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA
              781 CCGCAAAAAT CAGCGCGCAA ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA
              841 CGCGTTTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG
              901 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC
              961 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTTGTCCAT
             1021 ATTTGCCCAC GTTTAAATCA AACTGCTGAA ACTCACCCAG GATTGGCCTG GAGACGAAAA
             1081 CCATATTCTT CAATTAAACC TTAGAAATAG CCAGGTTTCC ACCGGTTAAC ACACCGGCCA
            1141 CCAATT
            //
            Primer: LEU2pro_F
            Sequence file: RDB12283_A3Jmc_v2.seq check
            >06519_12283_A3Jm_LEU2pro_F_G07_19.ab1
                1 ATTTTGCAGT GAAATATTAT TTAAGGACCT ATTGTTTTTT CCAATAGGTG GTTAGCAATC
               61 GTCTTACTTT CTAACTTTTC TTACCTTTTA CATTTCAGCA ATATATATAT ATATTTCAAG
              121 GATATACCAT TCTAATGTCT GCCCCTAAGA AGATCGTCGT TTTGCCAGGT GACCACGTTG
              181 GTCAAGAAAT CACAGCCGAA GCCATTAAGG TTCTTAAAGC TATTTCTGAT GTTCGTTCCA
              241 ATGTCAAGTT CGATTTCGAA AATCATTTAA TTGGTGGTGC TGCTATCGAT GCTACAGGTG
              301 TTCCACTTCC AGATGAGGCG CTGGAAGCCT CCAAGAAGGC TGATGCCGTT TTGTTAGGTG
              361 CTGTGGGTGG TCCTAAATGG GGTACCGGTA GTGTTAGACC TGAACAAGGT TTACTAAAAA
              421 TCCGTAAAGA ACTTCAATTG TACGCCAACT TAAGACCATG TAACTTTGCA TCCGACTCTC
              481 TTTTAGACTT ATCTCCAATC AAGCCACAAT TTGCTAAAGG TACTGACTTC GTTGTTGTCA
              541 GAGAATTAGT GGGAGGTATT TACTTTGGTA AGAGAAAGGA AGACGATGGT GATGGTGTCG
              601 CTTGGGATAG TGAACAATAC ACCGTTCCAG AAGTGCAAAG AATCACAAGA ATGGCCGCTT
              661 TCATGGCCCT ACAACATGAG CCACCATTGC CTATTTGGTC CTTGGATAAA GCTAATGTTT
              721 TGGCCTCTTC AAGATTATGG AGAAAAACTG TGGAGGAAAC CATCAAGAAC GAATTCCCTA
              781 CATTGAAAGT TCAACATCAA TTGATTGATT CTGCCGCCAT GATCCTAGTT AAGAACCCAA
              841 CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGACATCATC TCCGATGAAG
              901 CCTCGTATCC CAGGTTCCTT GGGTTTGTGC CATCTGCGTC TTGGCCTCTT TGCCAGACAG
              961 AACACGCAAT TTGGTTTGTA CGAACATGCA ACGATCTGCT CAGATTGCAA GATAAGTCAC
             1021 CTATCGCCAC CTACTTCTGC TGCATGAATG TGAATGCATG ACTGCCTGAG AGTTAAAGGC
            1081 AT
            //
            Primer: f1ori_R3
            Sequence file: RDB12283_A3Jmd_v2.seq check
            >06519_12283_A3Jm_f1ori_R3_H07_22.ab1
                1 GGGGGGGTCC TGGCGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC
              241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG
              301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC
              361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT
              421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA
              481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG
              541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT
              601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT
              661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG
              721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC
              781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG
              841 TTTTGATAAA TTGTAAAACT CTTACTCAAA AAAAGAAATG TTAAAAACTA ATTTAACCTT
              901 TTTTATAAAG GCTCATGGGC GTTAAAGAGG ATAGTAAAAA TTTCTACAAT TTTTTTAATT
              961 TAATGTCACA ATTTGTCTTT GCGAATATAT TTCTATTAAA TTTTCGCTAA CTGCTGTTTT
             1021 AAGTACTATT AACTTTCAAT ATGCCTTATA TGGCAACCTT TCAGTCATGA GGAATGCATA
            1081 TTTTTGGCCA ATCATCTGGT ACTGGAGCGC TTTTTTAT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Stigliano, I.D., Glucosidase II and N-glycan mannose content regulate the half-lives of monoglucosylated species in vivo. Mol. Biol. Cell 22 (11): 1810-1823 (2011). PMID 21471007.
            user_report Stigliano I.D., Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals. Mol. Biol. Cell, 20, 3974-3984 (2009). PMID 19605557.

            2022.05.18

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