Resource data sheet
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pREP1-ccdB2

S. pombe vector with nmt1 promoter

Catalog number RDB06519
Resource name pREP1-ccdB2
Clone info. S. pombe vector with nmt1 promoter.
Gateway compatible
Vector backbone pREP1 (Plasmid)
Selectable markers Amp^r, Cm^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06519 pREP1-ccdB2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06519z.seq from Depositor

Featured content

Featured content Dnaconda's recommendation EXR0025j (Japanese text)
Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12283_A3Jmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB12283_A3Jma_v2.seq
>06519_12283_A3Jm_nmt1Fw_E04_13.ab1
    1 GAAATTTATT TAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
  181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
  241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
  301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT
  361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA
  421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
  481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT
  541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA
  601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG
  661 GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT
  721 ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC
  781 GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT
  841 GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT TCTACACATA
  901 TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCTGGCCT ATTTCCCTAA AGGGTTTATT
  961 GAGAATATGT TTTTTCGTCT CAGCCCAATC CCTGGTGAGT TTCACCAGTT TTGATTTAAA
 1021 CGTGGCCAAT ATGGACAACT TCTTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC
 1081 AAGGCGACAG GTTGCCTGAA TGCGCTGACC GAATTCAGAT TCATCATGCC CGGATTTGGT
1141 TGAATGGGCC T
//
Primer: nmt1Rv RDB12283_A3Jmb_v2.seq
>06519_12283_A3Jm_nmt1Rv_F04_16.ab1
    1 GGGGCGCCCG GGGCCTGGAG ATCTCATGAT ATCACCACTT TGTACAAGAA AGCTGAACGA
   61 GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT
  121 ACATAATACT GTAAAACACA ACATATCCAG TCACTATGGT CGACCTGCAG ACTGGCTGTG
  181 TATAAGGGAG CCTGACATTT ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC
  241 ATTTTCGCGG TGGCTGAGAT CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC
  301 CATATCGGTG GTCATCATGC GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC
  361 ACGGGAGACT TTATCTGACA GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC
  421 GGGCGTGTCA ATAATATCAC TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT
  481 ATAGGTGTAA ACCTTAAACT GCATTTCACC AGCCCCTGTT CTCGTCAGCA AAAGAGCCGT
  541 TCATTTCAAT AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT
  601 CGGCACGCAG ACGACGGGCT TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA
  661 CATCATATAT GCCTTGAGCA ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG
  721 CTTCATAGCA TACCTCTTTT TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA
  781 CCGCAAAAAT CAGCGCGCAA ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA
  841 CGCGTTTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG
  901 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC
  961 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTTGTCCAT
 1021 ATTTGCCCAC GTTTAAATCA AACTGCTGAA ACTCACCCAG GATTGGCCTG GAGACGAAAA
 1081 CCATATTCTT CAATTAAACC TTAGAAATAG CCAGGTTTCC ACCGGTTAAC ACACCGGCCA
1141 CCAATT
//
Primer: LEU2pro_F RDB12283_A3Jmc_v2.seq
>06519_12283_A3Jm_LEU2pro_F_G07_19.ab1
    1 ATTTTGCAGT GAAATATTAT TTAAGGACCT ATTGTTTTTT CCAATAGGTG GTTAGCAATC
   61 GTCTTACTTT CTAACTTTTC TTACCTTTTA CATTTCAGCA ATATATATAT ATATTTCAAG
  121 GATATACCAT TCTAATGTCT GCCCCTAAGA AGATCGTCGT TTTGCCAGGT GACCACGTTG
  181 GTCAAGAAAT CACAGCCGAA GCCATTAAGG TTCTTAAAGC TATTTCTGAT GTTCGTTCCA
  241 ATGTCAAGTT CGATTTCGAA AATCATTTAA TTGGTGGTGC TGCTATCGAT GCTACAGGTG
  301 TTCCACTTCC AGATGAGGCG CTGGAAGCCT CCAAGAAGGC TGATGCCGTT TTGTTAGGTG
  361 CTGTGGGTGG TCCTAAATGG GGTACCGGTA GTGTTAGACC TGAACAAGGT TTACTAAAAA
  421 TCCGTAAAGA ACTTCAATTG TACGCCAACT TAAGACCATG TAACTTTGCA TCCGACTCTC
  481 TTTTAGACTT ATCTCCAATC AAGCCACAAT TTGCTAAAGG TACTGACTTC GTTGTTGTCA
  541 GAGAATTAGT GGGAGGTATT TACTTTGGTA AGAGAAAGGA AGACGATGGT GATGGTGTCG
  601 CTTGGGATAG TGAACAATAC ACCGTTCCAG AAGTGCAAAG AATCACAAGA ATGGCCGCTT
  661 TCATGGCCCT ACAACATGAG CCACCATTGC CTATTTGGTC CTTGGATAAA GCTAATGTTT
  721 TGGCCTCTTC AAGATTATGG AGAAAAACTG TGGAGGAAAC CATCAAGAAC GAATTCCCTA
  781 CATTGAAAGT TCAACATCAA TTGATTGATT CTGCCGCCAT GATCCTAGTT AAGAACCCAA
  841 CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGACATCATC TCCGATGAAG
  901 CCTCGTATCC CAGGTTCCTT GGGTTTGTGC CATCTGCGTC TTGGCCTCTT TGCCAGACAG
  961 AACACGCAAT TTGGTTTGTA CGAACATGCA ACGATCTGCT CAGATTGCAA GATAAGTCAC
 1021 CTATCGCCAC CTACTTCTGC TGCATGAATG TGAATGCATG ACTGCCTGAG AGTTAAAGGC
1081 AT
//
Primer: f1ori_R3 RDB12283_A3Jmd_v2.seq
>06519_12283_A3Jm_f1ori_R3_H07_22.ab1
    1 GGGGGGGTCC TGGCGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC
  241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG
  301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC
  361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT
  421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA
  481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG
  541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT
  601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT
  661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG
  721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC
  781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG
  841 TTTTGATAAA TTGTAAAACT CTTACTCAAA AAAAGAAATG TTAAAAACTA ATTTAACCTT
  901 TTTTATAAAG GCTCATGGGC GTTAAAGAGG ATAGTAAAAA TTTCTACAAT TTTTTTAATT
  961 TAATGTCACA ATTTGTCTTT GCGAATATAT TTCTATTAAA TTTTCGCTAA CTGCTGTTTT
 1021 AAGTACTATT AACTTTCAAT ATGCCTTATA TGGCAACCTT TCAGTCATGA GGAATGCATA
1081 TTTTTGGCCA ATCATCTGGT ACTGGAGCGC TTTTTTAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Stigliano, I.D., Glucosidase II and N-glycan mannose content regulate the half-lives of monoglucosylated species in vivo. Mol. Biol. Cell 22 (11): 1810-1823 (2011). PMID 21471007.
user report Stigliano I.D., Glucosidase II beta subunit modulates N-glycan trimming in fission yeasts and mammals. Mol. Biol. Cell, 20, 3974-3984 (2009). PMID 19605557.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl