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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pYFH1-ccdB2

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06515
Resource name pYFH1-ccdB2
Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06515 pYFH1-ccdB2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06515z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB09079_A8Eip1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB09079_A8Eia.seq check
            >06515_09079_A8Ei_nmt1Fw_10_F01_16_ABI24.ab1
                1 ATAGAATTTT ATTAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
              241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
              301 ATCCGGATAT CAACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT
              361 ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA
              421 CATATCCAGT CACTATGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
              481 GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG
              541 CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA
              601 AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC
              661 TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT
              721 TTATTCACAT TCTTGCCCGC CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG
              781 ACGGTGAGCT GGTGATATGG GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA
              841 CTGAAACGTT TTCATCGCTC TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA
              901 TATATTCGCA AGATGTGGCG TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA
              961 TTGAGAATAT GTTTTTCGTC TCAGCCAATC CCTGGGTGAG TTTCACCCAG TTTTGAATTT
             1021 AAACGTGGGC CAATATGGGA ACAACTTTCT TTCGCCCCCC GTTTTCACCA TGGGCAAATA
            1081 TTATACGCAG CCGACCAGTG CTGATGCCGC TGGGCGAATT CCAAGTTCCA GTA
            //
            Primer: nmt1ter_R
            Sequence file: RDB09079_A8Eib.seq check
            >06515_09079_A8Ei_nmt1ter_R_10_G01_19_ABI24.ab1
                1 GACTTTTTAG TAACTCATGT ATAAACGTTT TGGGTTCATA TTTTTGGGCC ACGTGGTTTA
               61 TAAAAATTCT TAACTACACC ACTCGGTATC CCGCACCCGT CTACGTTTCT ACGATTTCGA
              121 GCTAATATTG AGTAAAGTGA TTAGCAAAAA AAATAAAAGT ACTCGTTGTC GGAGATCAAG
              181 AATTTTTCTA TTATCTCATC TAAACCACTT TCTAAAAGCG AAAAACAAAA TCGTAATATG
              241 CAGCTTGAAT GGGCTTCCAT AGTTTGAAAG AAAAACCCTA GCAGTACTGG CAAGGGAGAC
              301 ATTCCTTTTA CCCTTAGTTA GTTAGCGGCC GCCCGGGCTC GAGATCTCAG TGGTGATGGT
              361 GGTGATGTTT GTCATCGTCG TCCTTGTAGT CCATGGGCTT GTACAGCTCG TCCATGCCGA
              421 GAGTGATCCC GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG
              481 GGTCTTTGCT CAGGGCGGAC TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC
              541 CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA
              601 TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT
              661 AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT
              721 TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT
              781 CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC
              841 CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA
              901 AGCACTGCAG GCCGTAGCCG AAAGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC
              961 CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGTG GCATCGCCCT CGCCCTCGCC
             1021 GGACACGCTG AACCTTGTGG CCGTTTACGT CGCCGTTCCA GCCTCGACCA AGGGATGGGC
             1081 ACCACCCCCG ATTGACAGGC TTCCTTCGAC CTTTGGCCTC CACCATGTGA TTATTCGAAC
            1141 GAC
            //
            Primer: EYFP-N
            Sequence file: RDB09079_A8Eic.seq check
            >06515_09079_A8Ei_EGFP_N_C02_03_ABI08.ab1
                1 GGACCCCCGG GGGGTGACAG CTCCTCGCCC TTGGCTCACC ATGATAATCA ACCACTTTGT
               61 ACAAGAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT
              121 TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA TATCCAGTCA CTATGGTCGA
              181 CCTGCAGACT GGCTGTGTAT AAGGGAGCCT GACATTTATA TTCCCCAGAA CATCAGGTTA
              241 ATGGCGTTTT TGATGTCATT TTCGCGGTGG CTGAGATCAG CCACTTCTTC CCCGATAACG
              301 GAGACCGGCA CACTGGCCAT ATCGGTGGTC ATCATGCGCC AGCTTTCATC CCCGATATGC
              361 ACCACCGGGT AAAGTTCACG GGAGACTTTA TCTGACAGCA GACGTGCACT GGCCAGGGGG
              421 ATCACCATCC GTCGCCCGGG CGTGTCAATA ATATCACTCT GTACATCCAC AAACAGACGA
              481 TAACGGCTCT CTCTTTTATA GGTGTAAACC TTAAACTGCA TTTCACCAGC CCCTGTTCTC
              541 GTCAGCAAAA GAGCCGTTCA TTTCAATAAA CCGGGCGACC TCAGCCATCC CTTCCTGATT
              601 TTCCGCTTTC CAGCGTTCGG CACGCAGACG ACGGGCTTCA TTCTGCATGG TTGTGCTTAC
              661 CAGACCGGAG ATATTGACAT CATATATGCC TTGAGCAACT GATAGCTGTC GCTGTCAACT
              721 GTCACTGTAA TACGCTGCTT CATAGCATAC CTCTTTTTGA CATACTTCGG GTATACATAT
              781 CAGTATATAT TCTTATACCG CAAAAATCAG CGCGCAAATA CGCATACTGT TATCTGGCTT
              841 TTAGTAAGCC GGATCCAGAT CTTTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA
              901 ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG
              961 CCAGCGGCAT CAGCACCTTG TCGCCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG
             1021 GCGAAGAAGT TGTCCATATT GGCCACGTTT AATCAAACTG GTGAAACTCA CCCAGGATTG
             1081 GCCTGGAGAC GAAAAACATA TCTCATAAGC CTTAGGGATA GGCCAGTTTT TCCACCGGTA
            1141 ACACCGCCCC ACATCTCTGG CCG
            //
            Primer: f1ori_F
            Sequence file: RDB09079_A8Eid.seq check
            >06515_09079_A8Ei_f1ori_F_H02_08_ABI08.ab1
                1 CAGGGGGCCT TGGCGCTGGC AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG
               61 CGCTTAATGC GCCGCTACAG GGCGCGTACT ATGGTTGCTT TGACGTATGC GGTGTGAAAT
              121 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG
              181 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
              241 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
              301 TAAAACGACG GCCAGTGAAT TCCAACCTTC CAATTCATTA AATCGAGTCC TCCATCAGAT
              361 GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG
              421 TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA
              481 AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA TAGTTTTCTA
              541 TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA GCGGCAGCGT
              601 TCATTATGGT ATATAAAAGA TAAAAAGTAG GGGCAAGCGA GACAGAATGG GATACAAGGG
              661 CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTGT AATTTTATTT
              721 CTCATTGTTT TACTTAAAAT GAATGCGAAT TAGAGAAAAC TTCAACGAAA TGTCAAAATA
              781 AGCTCAGCAA AATATACAAT TTTAGGGAAA GCGATCAGCA ACTTATTCTC CGTTGTAGAT
              841 TCTTTTTCTT CATAAATTCC AACAATGTAA CTGCCAATTG ATTTTTCTCT AGATGCTAAT
              901 AAATTGAAAC TAATGAATAT AATAACACAG GTTTTGATAA ATTGTAAACT CTTACTCAAA
              961 AAAAGAATGT TAAAACTAAT TTAACCTTTT TATAAAGGCT CATGGCGTTA AAGAGGATAG
             1021 TAAAAATTCT ACAATTTTTT AATTTAATGT CACATTGTCT TGCGAATATA ATCTATAATT
             1081 TTCGCTAACT GCTGTTTAGT ACTATACTTC ATATGCTTAA TGCACTAATT CATGAGGATG
            1141 CATATTTTTG CATTCTTTTC TTGTACCGTG AGGCGG
            //
            Primer: LEU2pro_F
            Sequence file: RDB09078_A8Eid.seq check
            1

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl