Resource data sheet
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pCFH1-ccdB2

S. pombe 3 ter end CFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06514
Resource name pCFH1-ccdB2
Clone info. S. pombe 3 ter end CFP-FLAG-His6-tagged vector with nmt1 promoter.
Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


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RDB06514 pCFH1-ccdB2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06514z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12298_A5L2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12298_A5L2a.seq
>06514_12298_A5L2_nmt1Fw_A12_03.ab1
    1 TGTTATTTAA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
  181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
  241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
  301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT
  361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA
  421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
  481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT
  541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA
  601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG
  661 GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT
  721 ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC
  781 GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT
  841 GAACGTTTCA TCGCTCTGGA GTGAATACAC GACGATTCGC AGTTCTACAA CATAATATCG
  901 CAGATGTGGC GTGTACGTGA AAACTGCTAT ACCCTAAACG TATGAGAATA TGTTTCGTCT
  961 CAGCCAATCT GGGTGGTCAC AGTTTGAATA CCTGGCCAAT GCCAGTCTGC CGTACGATGC
1021 ATTTAGCAAG CAAGCTTAGG CCTGCTATCG GTACATGCG
//
Primer: nmt1Rv | Sequence: RDB12298_A5L2b.seq
>06514_12298_A5L2_nmt1Rv_B12_06.ab1
    1 AGGGGGGGCC GGGGGCTCGA AATCTCAGTG GTGATGGTGG TGATGTTTGT CATCGTCGTC
   61 CTTGTAGGTC CATGGGCTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA
  121 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT
  181 GGGTGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT
  241 GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT
  301 TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGATGTAGT
  361 TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT
  421 CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC
  481 CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA
  541 AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCCCAGGTCA
  601 GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA
  661 GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC
  721 CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGGCACCA CCCCGGTGAA CAGCTCCTCG
  781 CCCTTGCTCA CCCATGATAT CAACCACTTT GTACAAGAAA GCTGAACGAG AAAACGTAAA
  841 ATGATATAAA ATATCAATAT ATTAAATTTA GATTTTGCAT AAAAACAGAC TACATAATAC
  901 TGTAAAACAC AACATATCCA GTCACTATGG TCGACCTGCA GACTGGCTGT GTAATAAGGG
  961 GAGCCTGACA TTTATATTCC CCAGAACAAT CAGGTTAATG GCGTTTTTGA ATGTCAATTT
 1021 TTCCGCGGTG GGCTTGAGAT CCAGCTCACT TTCTTTCCCC GGATTAACGG AAAACCGGCA
 1081 CACTTGGTCC ATTATTCGGG TGGTTCATCA TGCGGCCAGC CTTTTACATC CTCCGAATAT
 1141 TGCACCACCG GGTAAAAGGT TCAGCGGAGA ACTTTTAATT CTGACAGCAG ACGTGCAATC
1201 GTGGGCCCCA GAGGTGTGT
//
Primer: LEU2pro_F | Sequence: RDB12298_A5L2c.seq
>06514_12298_A5L2_LEU2pro_F_C12_09.ab1
    1 ATCATGCAGG TGAAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
   61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
  901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGC CTCTTTGCCA
  961 GACAAGAACA CCGCATTTGG TTTGTACGAA CCCATGCCAC GGGTTCTGCT CCAGATTTGC
 1021 CAAAAGAATA AAGGTCAACC CTATCGCAAC TATCTGTCTG CTGCAATGAT GGTGAAATGG
 1081 TCATGACTTG CCCTGAAGGA AGGTAGGTCA ATGGAGATGC AGTCAAAAAG GTTTGGAATG
1141 CAGGTTATTT CAG
//
Primer: f1ori_R3 | Sequence: RDB12298_A5L2d.seq
>06514_12298_A5L2_f1ori_R3_D12_12.ab1
    1 GTTTGTGGTG CTTTTCGTTG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
   61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
  241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
  301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
  361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
  421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
  481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
  541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
  601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
  661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
  721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
  781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG
  841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT
  901 TATAAAGGCT CATGGCGTTA AAGAGGATAG TAAAAATTCT ACAATTTTTT AATTTAATGT
  961 CACAATTGTC TTGCGAATAT AATTCTATTA ATTTTTCGCT AACTGCTGTT TTAGTACTAT
 1021 TACTTTCAAT ATGCCTTATA TGCAACTTTA ATTCATGAAG AAATGCAATA TTTTTTGGCA
 1081 ATCTTTCTGT ACTTGAAGCG CGTTTTAATC AATTAAGTAC TCAATATTAA TTTTTTTCAA
1141 GGTAAAAGGG CCAAAATAAA ATTAAA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

GNP_filter3_RDBDEP_html_190927.pl