Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCFH1-ccdB2

S. pombe 3 ter end CFP-FLAG-His6-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12298_A5L2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB12298_A5L2a.seq
>06514_12298_A5L2_nmt1Fw_A12_03.ab1     1 TGTTATTTAA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG    61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG   121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT   181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT   241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT   301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT   361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA   421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC   481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT   541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA   601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG   661 GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT   721 ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC   781 GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT   841 GAACGTTTCA TCGCTCTGGA GTGAATACAC GACGATTCGC AGTTCTACAA CATAATATCG   901 CAGATGTGGC GTGTACGTGA AAACTGCTAT ACCCTAAACG TATGAGAATA TGTTTCGTCT   961 CAGCCAATCT GGGTGGTCAC AGTTTGAATA CCTGGCCAAT GCCAGTCTGC CGTACGATGC 1021 ATTTAGCAAG CAAGCTTAGG CCTGCTATCG GTACATGCG //
Primer: nmt1Rv Sequence file: RDB12298_A5L2b.seq
>06514_12298_A5L2_nmt1Rv_B12_06.ab1     1 AGGGGGGGCC GGGGGCTCGA AATCTCAGTG GTGATGGTGG TGATGTTTGT CATCGTCGTC    61 CTTGTAGGTC CATGGGCTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA   121 CGAACTCCAG CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT   181 GGGTGCTCAG GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT   241 GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT   301 TGGCCTTGAT GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGATGTAGT   361 TGTACTCCAG CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT   421 CGATGCGGTT CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC   481 CGTCGTCCTT GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA   541 AGAAGTCGTG CTGCTTCATG TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCCCAGGTCA   601 GGGTGGTCAC GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA   661 GGGTCAGCTT GCCGTAGGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGC   721 CGTTTACGTC GCCGTCCAGC TCGACCAGGA TGGGGCACCA CCCCGGTGAA CAGCTCCTCG   781 CCCTTGCTCA CCCATGATAT CAACCACTTT GTACAAGAAA GCTGAACGAG AAAACGTAAA   841 ATGATATAAA ATATCAATAT ATTAAATTTA GATTTTGCAT AAAAACAGAC TACATAATAC   901 TGTAAAACAC AACATATCCA GTCACTATGG TCGACCTGCA GACTGGCTGT GTAATAAGGG   961 GAGCCTGACA TTTATATTCC CCAGAACAAT CAGGTTAATG GCGTTTTTGA ATGTCAATTT  1021 TTCCGCGGTG GGCTTGAGAT CCAGCTCACT TTCTTTCCCC GGATTAACGG AAAACCGGCA  1081 CACTTGGTCC ATTATTCGGG TGGTTCATCA TGCGGCCAGC CTTTTACATC CTCCGAATAT  1141 TGCACCACCG GGTAAAAGGT TCAGCGGAGA ACTTTTAATT CTGACAGCAG ACGTGCAATC 1201 GTGGGCCCCA GAGGTGTGT //
Primer: LEU2pro_F Sequence file: RDB12298_A5L2c.seq
>06514_12298_A5L2_LEU2pro_F_C12_09.ab1     1 ATCATGCAGG TGAAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT    61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA   121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT   181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC   241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT   301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT   361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA   421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT   481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC   541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC   601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT   661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT   721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT   781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA   841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA   901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGC CTCTTTGCCA   961 GACAAGAACA CCGCATTTGG TTTGTACGAA CCCATGCCAC GGGTTCTGCT CCAGATTTGC  1021 CAAAAGAATA AAGGTCAACC CTATCGCAAC TATCTGTCTG CTGCAATGAT GGTGAAATGG  1081 TCATGACTTG CCCTGAAGGA AGGTAGGTCA ATGGAGATGC AGTCAAAAAG GTTTGGAATG 1141 CAGGTTATTT CAG //
Primer: f1ori_R3 Sequence file: RDB12298_A5L2d.seq
>06514_12298_A5L2_f1ori_R3_D12_12.ab1     1 GTTTGTGGTG CTTTTCGTTG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG    61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC   121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT   181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC   241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA   301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT   361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT   421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA   481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG   541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA   601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT   661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA   721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA   781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG   841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT   901 TATAAAGGCT CATGGCGTTA AAGAGGATAG TAAAAATTCT ACAATTTTTT AATTTAATGT   961 CACAATTGTC TTGCGAATAT AATTCTATTA ATTTTTCGCT AACTGCTGTT TTAGTACTAT  1021 TACTTTCAAT ATGCCTTATA TGCAACTTTA ATTCATGAAG AAATGCAATA TTTTTTGGCA  1081 ATCTTTCTGT ACTTGAAGCG CGTTTTAATC AATTAAGTAC TCAATATTAA TTTTTTTCAA 1141 GGTAAAAGGG CCAAAATAAA ATTAAA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl