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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHFY1-ccdB2

S. pombe 5 ter end His6-FLAG-YFP-tagged vector with nmt1 promoter

Catalog number RDB06513
Resource name pHFY1-ccdB2
Clone info. S. pombe 5 ter end His6-FLAG-YFP-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06513 pHFY1-ccdB2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06513z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12296_A8Eip1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB12296_A8Eia.seq check
            >06513_12296_A8Ei_nmt1Fw_10_C01_07_ABI24.ab1
                1 AGCAATTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT
               61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA
              121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATATAT
              181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
              241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
              301 TATGCATCAC CACCATCATC ATATGGATTA TAAGGATGAT GACGATAAAC CCATGGTGAG
              361 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
              421 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
              481 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
              541 CACCTTCGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA
              601 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
              661 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
              721 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
              781 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
              841 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
              901 CCAGCAGAAC ACCCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
              961 GCTACCAGTC CGCCCTGAGC AAAGACCCCC AACGAGAAGC GCGATCACAT GGGTCCCTGC
             1021 TGGAGTTTCG TGACCGCCGC CGGGATCACT CTCGGCCATG GACGAGCTGT ACAAGGATAT
            1081 CACAGGTTGG TACCAAAAGC TGACGAAGAA CCGTTAAAAA GTGGAAT
            //
            Primer: nmt1ter_R
            Sequence file: RDB12296_A8Eib.seq check
            >06513_12296_A8Ei_nmt1ter_R_10_D01_10_ABI24.ab1
                1 TAACTTTTTT GTTACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT
               61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG
              121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA
              181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT
              241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA
              301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA TGATATCACC
              361 ACTTTGTACA AGAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT
              421 TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA
              481 TGGTCGACCT GCAGACTGGC TGTGTATAAG GGAGCCTGAC ATTTATATTC CCCAGAACAT
              541 CAGGTTAATG GCGTTTTTGA TGTCATTTTC GCGGTGGCTG AGATCAGCCA CTTCTTCCCC
              601 GATAACGGAG ACCGGCACAC TGGCCATATC GGTGGTCATC ATGCGCCAGC TTTCATCCCC
              661 GATATGCACC ACCGGGTAAA GTTCACGGGA GACTTTATCT GACAGCAGAC GTGCACTGGC
              721 CAGGGGGATC ACCATCCGTC GCCCGGGCGT GTCAATAATA TCACTCTGTA CATCCACAAA
              781 CAGACGATAA CGGCTCTCTC TTTTATAGGT GTAAACCTTA AACTGCATTT CACCAGCCCC
              841 TGTTCTCGTC AGCAAAAGAG CCGTTCATTT CAATAAACCG GGCGACCTCA GCCATCCCTT
              901 CCTGATTTTC CGCTTTCCAG CGTTCGGCAC GCAGACGACG GGCTTCATTC TGCATGGTTG
              961 TGCTTACCAG ACCGGAGATA TTGACATCAT ATATGCCTTG AGCAACTGAT AGCTGTCGCT
             1021 GTCAACTGTC ACTGTAATAC GCTGCTTCAT AGCATACCCT CTTTTTTGAA CATACTTCGG
            1081 GTATACATAT CCAGTATATA TTGTTATAAC CGGCCAAAAA AATTCCAGTC GGCGCCCA
            //
            Primer: EYFP-C_F
            Sequence file: RDB12296_A8Eic.seq check
            >06513_12296_A8Ei_EYFP-C_F_10_E01_13_ABI24.ab1
                1 GGTTCCAAGC ACCCCCACCG AGGAAGGCGC CGGATCACAT GGTCCTGCTG GGAGTTCGTG
               61 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGGATATCAC AAGTTTGTAC
              121 AAAAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG
              181 CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA TCCAGTCACT ATGGCGGCCG
              241 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATAATGTG TGGATTTTGA
              301 GTTAGGATCC GTCGAGATTT TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG
              361 GATATACCAC CGTTGATATA TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT
              421 CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA
              481 CCGTAAAGAA AAATAAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG
              541 ATGAAATGCT CATCCGGAAA TTCCGTATGG CAATGAAAGA CGGTGAAGCT GGTGATATGG
              601 GAATAGTGTT CAACCCTTGG TTAACCGTTT TTCATTGAGC AAACTGGAAA CGTTTTCTTC
              661 GCTTCTGGAG TGAATACCCA CGACCAATTT CCGGAGTTTT CACACCATAT TTTTCCAAAA
              721 AGGGGGCGGG TTACGGGAAA ACCCGTCCCT TTTTCCCTAA AGGGGTAATT TGAAAAAAAT
              781 GTTTTTTCCC CCCCCCAATC CCGGTGGGAA TTCCCCCAGT TTGATATAAA CAGGGCACCA
              841 ATGGGAAACT TCTTCTCCCC CCGTTTTTAC ATGGGGGAAA TATATATCCA CAGGGCAGAC
            901 AGGGTGTGAA GCT
            //
            Primer: f1ori_F
            Sequence file: RDB12296_A8Eid.seq check
            >06513_12296_A8Ei_f1ori_F_B02_02_ABI08.ab1
                1 AGGGGGGGCC TTTAGGGCGC GGGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC
               61 CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTACTATGG TTGCTTTGAC GTATGCGGTG
              121 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG
              181 GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC
              241 GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG
              301 ACGTTGTAAA ACGACGGCCA GTGAATTCCA ACCTTCCAAT TCATTAAATC GAGTCCTCCA
              361 TCAGATGATT TACAAGGTTT TGCATAGAAT CCCTAAGTAA GAGAAGTTGT TAGTGATTTC
              421 TTCAAGTTTA CTAAAATTTA CATACAAATG GATGATCTCT TCTCCATTGT TTCCTTTCTT
              481 CTTGCAAACG CGTTTTACAA AGAGATGACA TGCTATTTTC TGATTATTTT TTTCTATAGT
              541 TTTCTATTTT GTACGTCCTT GGGAGTCCGA AATGTAAAAT CGGGGTATGG TTAGTAGCGG
              601 CAGCGTTCAT TATGGTATAT AAAAGATAAA AAGTAGGGGC AAGCGAGACA GAATGGGATA
              661 CAAGGGCATC GTCTATAATT ATAGCTAAAA ATTGTATTTT AATTTGTATT TTTTGTAATT
              721 TTATTTCTCA TTGTTTTACT TAAAATGAAT GCGAATTAGA GAAAACTTCA ACGAAATGTC
              781 AAAATAAGCT CAGCAAAATA TACAATTTTA GGGAAAGCGA TCAGCAACTT ATTCTCCGTT
              841 GTAGATTCTT TTTCTTCATA AATTCCAACA ATGTAACTGC CAATTGATTT TTCTCTAGAT
              901 GCTAATAAAT TGAAAACTAA TGAATATAAT AACACAGGTT TTGATAATTT GTAAACTCTT
              961 ACTCAAAAAA AAGAATGTTA AAACTAATTT AACCTTTTTA TAAGGCTCAT GGCGGTTAAA
             1021 GAGGATTAGT AAAAATTCTA CAATTTTTTT ATTTAATGTC ACAATTTGTC CTTGCGAATA
             1081 TAATTCTATT AATTTTCGCT AACTGCTGAT TTAGTACTAT TACTTCATAT GCCTAATATG
            1141 CACTTATCAT GGAAGAATGC CAAATTATTT TTTTTTGTCG CAAAACTC
            //
            Primer: LEU2pro_F
            Sequence file: RDB12296_A8Eie.seq check
            >06513_12296_A8Ei_LEUpro_F_A01_01_ABI08.ab1
                1 ATAATGGAGG TGAAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
               61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
              121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
              181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
              241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
              301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
              361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
              421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
              481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
              541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
              601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
              661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
              721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
              781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
              841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
              901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTGT TGCCATCTGC GTCCTTGGGC CTCTTTGCCA
              961 GACAAGACAC CGCATTTGGT TTGTACGAAC CATGCCACGG TTCTGCTCCA GATTTGCAAA
             1021 GATAGTCAAC CCTATCGCAC TATCTGTCTG CTGCATGATG TGAATGGTCA TTGGAACCTG
            1081 GCTGGCAGAA GATGGGTTAA AGC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl