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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGFH1-ccdB2

S. pombe 3 ter end GFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06509
Resource name pGFH1-ccdB2
Clone info. S. pombe 3 ter end GFP-FLAG-His6-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06509 pGFH1-ccdB2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06509z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12293_A3Jmp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB12293_A3Jma.seq check
            >06509_12293_A3Jm_nmt1Fw_A03_03.ab1
                1 CGAAATTTAA TTAAATCCAG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
               61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
              121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
              181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT
              241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT
              301 GGATCCGGAT ATCAACAAGT TTGTACAAAA AAGCTGAACG AGAAACGTAA AATGATATAA
              361 ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC
              421 AACATATCCA GTCACTATGG CGGCCGCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
              481 CGGCTCGTAT AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA
              541 AGCTAAAATG GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG
              601 TAAAGAACAT TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA
              661 GCTGGATATT ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC
              721 CTTTATTCAC ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA
              781 AGACGGTGAG CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA
              841 AACTGAAACG TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA
              901 CATATATTCG CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT
              961 TATTGAGAAT ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT
             1021 AAACGTGGCC CAATATGGAC AACTTTCTTT CGCCCCCGTT TTCACCATGG GGCAAATATA
             1081 TACGCAAGGG CGACAGGTTG CCTGAATGCG CTGCCGAATC CAGTCCATTC ATGCCGATTT
            1141 GTTGAATGGC CATCCAATGG TATCCCG
            //
            Primer: nmt1_Rv
            Sequence file: RDB12293_A3Jmb.seq check
            >06509_12293_A3Jm_nmt1Rv_B03_06.ab1
                1 ATCCGCGGTC TCGAGATCTC AGTGGTGATG GTGGTGATGT TTGTCATCGT CGTCCTTGTA
               61 GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC
              121 AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA
              181 CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA
              241 ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT
              301 GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC
              361 CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT
              421 ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC
              481 TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC
              541 ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA ACACCATAAC AGAAAGTAGT
              601 GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG CAAATAAATT TAAGGGTAAG
              661 TTTTCCGTAT GTTGCATCAC CTTCACCCTC TCCACTGACA GAAAATTTGT GCCCATTAAC
              721 ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG AAAAGTTCTT CTCCTTTACT
              781 CATGATATCA ACCACTTTGT ACAGAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
              841 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA TACTGTAAAA CACACATATC
              901 CAGTCCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG AGCCTGAACA TTTATATTCC
              961 CCAGAACATC AGTATGCGTT TTGGATGTCA TTTCGCGTGC TGAATCAGCA CTCTCCCGAT
             1021 ACGAACGGCA CTGGCATATC GTTGCATCAT GCCCAGCTAC ATCCAGATTG ACAACGGTAT
            1081 GTCACGAACT TACTGAACGG CGACG
            //
            Primer: EYFP-N
            Sequence file: RDB12293_A3Jmc.seq check
            >06509_12293_A3Jm_Cm_C_F_A01_01_ABI08.ab1
                1 CAACGTGAAA TGGATCGGCT TACTAAAAGC CAGATAACAG TATGCGTATT TGCGCGCTGA
               61 TTTTTGCGGT ATAAGAATAT ATACTGATAT GTATACCCGA AGTATGTCAA AAAGAGGTAT
              121 GCTATGAAGC AGCGTATTAC AGTGACAGTT GACAGCGACA GCTATCAGTT GCTCAAGGCA
              181 TATATGATGT CAATATCTCC GGTCTGGTAA GCACAACCAT GCAGAATGAA GCCCGTCGTC
              241 TGCGTGCCGA ACGCTGGAAA GCGGAAAATC AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA
              301 TTGAAATGAA CGGCTCTTTT GCTGACGAGA ACAGGGGCTG GTGAAATGCA GTTTAAGGTT
              361 TACACCTATA AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG TGATATTATT
              421 GACACGCCCG GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT GTCAGATAAA
              481 GTCTCCCGTG AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG CATGATGACC
              541 ACCGATATGG CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA TCTCAGCCAC
              601 CGCGAAAATG ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA AATGTCAGGC
              661 TCCCTTATAC ACAGCCAGTC TGCAGGTCGA CCATAGTGAC TGGATATGTT GTGTTTTACA
              721 GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT
              781 TTACGTTTCT CGTTCAGCTT TCTTGTACAA AGTGGTTGAT ATCATGAGTA AAGGAGAAAG
            841 AACTTTTCAC TGGGAGTTTG TCCCAAATTC TTTGTTGAA
            //
            Primer: f1ori_F
            Sequence file: RDB12293_A3Jmd.seq check
            >06509_12293_A3Jm_f1ori_F_B01_02_ABI08.ab1
                1 GGGGGGCGCC TACGCGGCTG GCAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC
               61 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTA CTATGGTTGC TTTGACGTAT GCGGTGTGAA
              121 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG
              181 CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA
              241 GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT
              301 TGTAAAACGA CGGCCAGTGA ATTCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG
              361 ATGATTTACA AGGTTTTGCA TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA
              421 AGTTTACTAA AATTTACATA CAAATGGATG ATCTCTTCTC CATTGTTTCC TTTCTTCTTG
              481 CAAACGCGTT TTACAAAGAG ATGACATGCT ATTTTCTGAT TATTTTTTTC TATAGTTTTC
              541 TATTTTGTAC GTCCTTGGGA GTCCGAAATG TAAAATCGGG GTATGGTTAG TAGCGGCAGC
              601 GTTCATTATG GTATATAAAA GATAAAAAGT AGGGGCAAGC GAGACAGAAT GGGATACAAG
              661 GGCATCGTCT ATAATTATAG CTAAAAATTG TATTTTAATT TGTATTTTTT GTAATTTTAT
              721 TTCTCATTGT TTTACTTAAA ATGAATGCGA ATTAGAGAAA ACTTCAACGA AATGTCAAAA
              781 TAAGCTCAGC AAAATATACA ATTTTAGGGA AAGCGATCAG CAACTTATTC TCCGTTGTAG
              841 ATTCTTTTTC TTCATAAAAT TCCAACAATG TAAAGTGCCA ATTGATTTTT CTCTAGATGC
              901 TAATAAATTG AAACTAATGA AATATAATAA CACAGGTTTT GATAAATTGT AAAACTCTTA
              961 CTCAAAAAAA GAATGTTAAA ACTAAATTTA ACCTTTTTAT AAAGGCTCAT GGGCGTTTAA
             1021 GAGGAATAGT AAAAAATTCT ACAATTTTTT AATTTTAATG TCACCAATTG TCCTGCCGCA
             1081 TATTATTCCT ATTTAATTTT CGCAACTGCC TGGTTTTAGT AACTATTACT CATATGCCTA
            1141 AATGCACCTT AGTTCATGAA AGGAATGGCA AATTATTGGT C
            //
            Primer: LEU2pro_F
            Sequence file: RDB12293_A3Jme.seq check
            >06509_12293_A3Jm_LEU2pro_F_C01_03_ABI08.ab1
                1 CAACAATTGA GTGACAATAT TATTTAAGGA CCTATTGTTT TTTCCAATAG GTGGTTAGCA
               61 ATCGTCTTAC TTTCTAACTT TTCTTACCTT TTACATTTCA GCAATATATA TATATATTTC
              121 AAGGATATAC CATTCTAATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG
              181 TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT
              241 CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG
              301 GTGTTCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGCTGATGCC GTTTTGTTAG
              361 GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA
              421 AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT
              481 CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG
              541 TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG
              601 TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG
            661 CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAAGCTA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl