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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGFH1-ccdB2 (#RDB06509)

S. pombe 3 ter end GFP-FLAG-His6-tagged vector with nmt1 promoter

Clone info. S. pombe 3 ter end GFP-FLAG-His6-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06509z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

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RDB06509 pGFH1-ccdB2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGFH1-ccdB2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06509).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12293_A3Jmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB12293_A3Jma.seq check
>06509_12293_A3Jm_nmt1Fw_A03_03.ab1
    1 CGAAATTTAA TTAAATCCAG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
   61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
  121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
  181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT
  241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT
  301 GGATCCGGAT ATCAACAAGT TTGTACAAAA AAGCTGAACG AGAAACGTAA AATGATATAA
  361 ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC
  421 AACATATCCA GTCACTATGG CGGCCGCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
  481 CGGCTCGTAT AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA
  541 AGCTAAAATG GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG
  601 TAAAGAACAT TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA
  661 GCTGGATATT ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC
  721 CTTTATTCAC ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA
  781 AGACGGTGAG CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA
  841 AACTGAAACG TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA
  901 CATATATTCG CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT
  961 TATTGAGAAT ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT
 1021 AAACGTGGCC CAATATGGAC AACTTTCTTT CGCCCCCGTT TTCACCATGG GGCAAATATA
 1081 TACGCAAGGG CGACAGGTTG CCTGAATGCG CTGCCGAATC CAGTCCATTC ATGCCGATTT
1141 GTTGAATGGC CATCCAATGG TATCCCG
//
Primer: nmt1_Rv
Sequence file: RDB12293_A3Jmb.seq check
>06509_12293_A3Jm_nmt1Rv_B03_06.ab1
    1 ATCCGCGGTC TCGAGATCTC AGTGGTGATG GTGGTGATGT TTGTCATCGT CGTCCTTGTA
   61 GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC
  121 AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA
  181 CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA
  241 ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT
  301 GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC
  361 CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT
  421 ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC
  481 TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC
  541 ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA ACACCATAAC AGAAAGTAGT
  601 GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG CAAATAAATT TAAGGGTAAG
  661 TTTTCCGTAT GTTGCATCAC CTTCACCCTC TCCACTGACA GAAAATTTGT GCCCATTAAC
  721 ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG AAAAGTTCTT CTCCTTTACT
  781 CATGATATCA ACCACTTTGT ACAGAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
  841 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA TACTGTAAAA CACACATATC
  901 CAGTCCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG AGCCTGAACA TTTATATTCC
  961 CCAGAACATC AGTATGCGTT TTGGATGTCA TTTCGCGTGC TGAATCAGCA CTCTCCCGAT
 1021 ACGAACGGCA CTGGCATATC GTTGCATCAT GCCCAGCTAC ATCCAGATTG ACAACGGTAT
1081 GTCACGAACT TACTGAACGG CGACG
//
Primer: EYFP-N
Sequence file: RDB12293_A3Jmc.seq check
>06509_12293_A3Jm_Cm_C_F_A01_01_ABI08.ab1
    1 CAACGTGAAA TGGATCGGCT TACTAAAAGC CAGATAACAG TATGCGTATT TGCGCGCTGA
   61 TTTTTGCGGT ATAAGAATAT ATACTGATAT GTATACCCGA AGTATGTCAA AAAGAGGTAT
  121 GCTATGAAGC AGCGTATTAC AGTGACAGTT GACAGCGACA GCTATCAGTT GCTCAAGGCA
  181 TATATGATGT CAATATCTCC GGTCTGGTAA GCACAACCAT GCAGAATGAA GCCCGTCGTC
  241 TGCGTGCCGA ACGCTGGAAA GCGGAAAATC AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA
  301 TTGAAATGAA CGGCTCTTTT GCTGACGAGA ACAGGGGCTG GTGAAATGCA GTTTAAGGTT
  361 TACACCTATA AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG TGATATTATT
  421 GACACGCCCG GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT GTCAGATAAA
  481 GTCTCCCGTG AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG CATGATGACC
  541 ACCGATATGG CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA TCTCAGCCAC
  601 CGCGAAAATG ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA AATGTCAGGC
  661 TCCCTTATAC ACAGCCAGTC TGCAGGTCGA CCATAGTGAC TGGATATGTT GTGTTTTACA
  721 GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT
  781 TTACGTTTCT CGTTCAGCTT TCTTGTACAA AGTGGTTGAT ATCATGAGTA AAGGAGAAAG
841 AACTTTTCAC TGGGAGTTTG TCCCAAATTC TTTGTTGAA
//
Primer: f1ori_F
Sequence file: RDB12293_A3Jmd.seq check
>06509_12293_A3Jm_f1ori_F_B01_02_ABI08.ab1
    1 GGGGGGCGCC TACGCGGCTG GCAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC
   61 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTA CTATGGTTGC TTTGACGTAT GCGGTGTGAA
  121 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG
  181 CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA
  241 GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT
  301 TGTAAAACGA CGGCCAGTGA ATTCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG
  361 ATGATTTACA AGGTTTTGCA TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA
  421 AGTTTACTAA AATTTACATA CAAATGGATG ATCTCTTCTC CATTGTTTCC TTTCTTCTTG
  481 CAAACGCGTT TTACAAAGAG ATGACATGCT ATTTTCTGAT TATTTTTTTC TATAGTTTTC
  541 TATTTTGTAC GTCCTTGGGA GTCCGAAATG TAAAATCGGG GTATGGTTAG TAGCGGCAGC
  601 GTTCATTATG GTATATAAAA GATAAAAAGT AGGGGCAAGC GAGACAGAAT GGGATACAAG
  661 GGCATCGTCT ATAATTATAG CTAAAAATTG TATTTTAATT TGTATTTTTT GTAATTTTAT
  721 TTCTCATTGT TTTACTTAAA ATGAATGCGA ATTAGAGAAA ACTTCAACGA AATGTCAAAA
  781 TAAGCTCAGC AAAATATACA ATTTTAGGGA AAGCGATCAG CAACTTATTC TCCGTTGTAG
  841 ATTCTTTTTC TTCATAAAAT TCCAACAATG TAAAGTGCCA ATTGATTTTT CTCTAGATGC
  901 TAATAAATTG AAACTAATGA AATATAATAA CACAGGTTTT GATAAATTGT AAAACTCTTA
  961 CTCAAAAAAA GAATGTTAAA ACTAAATTTA ACCTTTTTAT AAAGGCTCAT GGGCGTTTAA
 1021 GAGGAATAGT AAAAAATTCT ACAATTTTTT AATTTTAATG TCACCAATTG TCCTGCCGCA
 1081 TATTATTCCT ATTTAATTTT CGCAACTGCC TGGTTTTAGT AACTATTACT CATATGCCTA
1141 AATGCACCTT AGTTCATGAA AGGAATGGCA AATTATTGGT C
//
Primer: LEU2pro_F
Sequence file: RDB12293_A3Jme.seq check
>06509_12293_A3Jm_LEU2pro_F_C01_03_ABI08.ab1
    1 CAACAATTGA GTGACAATAT TATTTAAGGA CCTATTGTTT TTTCCAATAG GTGGTTAGCA
   61 ATCGTCTTAC TTTCTAACTT TTCTTACCTT TTACATTTCA GCAATATATA TATATATTTC
  121 AAGGATATAC CATTCTAATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG
  181 TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT
  241 CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG
  301 GTGTTCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGCTGATGCC GTTTTGTTAG
  361 GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA
  421 AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT
  481 CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG
  541 TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG
  601 TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG
661 CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAAGCTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles