S. pombe 3 ter end GFP-FLAG-His6-tagged vector with nmt1 promoter
Clone info. | S. pombe 3 ter end GFP-FLAG-His6-tagged vector with nmt1 promoter. |
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Comment | Gateway compatible. |
Vector backbone | pREP1 (Plasmid) |
Selectable markers | E. coli: Amp^r, Cm^r, ccdB. |
Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
Depositor|Developer | Yoshida, Minoru | Matsuyama, Akihisa | |
Sequence | RDB06509z.seq |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。 |
備考 | このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB06509 | pGFH1-ccdB2 | DNA solution |
Materials & Methods section:
The pGFH1-ccdB2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06509). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB12293_A3Jmp1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: nmt1Fw Sequence file: RDB12293_A3Jma.seq ![]() |
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>06509_12293_A3Jm_nmt1Fw_A03_03.ab1 1 CGAAATTTAA TTAAATCCAG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC 61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT 121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG 181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT 241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT 301 GGATCCGGAT ATCAACAAGT TTGTACAAAA AAGCTGAACG AGAAACGTAA AATGATATAA 361 ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC 421 AACATATCCA GTCACTATGG CGGCCGCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 481 CGGCTCGTAT AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA 541 AGCTAAAATG GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG 601 TAAAGAACAT TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA 661 GCTGGATATT ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC 721 CTTTATTCAC ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA 781 AGACGGTGAG CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA 841 AACTGAAACG TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA 901 CATATATTCG CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT 961 TATTGAGAAT ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT 1021 AAACGTGGCC CAATATGGAC AACTTTCTTT CGCCCCCGTT TTCACCATGG GGCAAATATA 1081 TACGCAAGGG CGACAGGTTG CCTGAATGCG CTGCCGAATC CAGTCCATTC ATGCCGATTT 1141 GTTGAATGGC CATCCAATGG TATCCCG // |
Primer: nmt1_Rv Sequence file: RDB12293_A3Jmb.seq ![]() |
>06509_12293_A3Jm_nmt1Rv_B03_06.ab1 1 ATCCGCGGTC TCGAGATCTC AGTGGTGATG GTGGTGATGT TTGTCATCGT CGTCCTTGTA 61 GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC 121 AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA 181 CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA 241 ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT 301 GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC 361 CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT 421 ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC 481 TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC 541 ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA ACACCATAAC AGAAAGTAGT 601 GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG CAAATAAATT TAAGGGTAAG 661 TTTTCCGTAT GTTGCATCAC CTTCACCCTC TCCACTGACA GAAAATTTGT GCCCATTAAC 721 ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG AAAAGTTCTT CTCCTTTACT 781 CATGATATCA ACCACTTTGT ACAGAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA 841 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA TACTGTAAAA CACACATATC 901 CAGTCCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG AGCCTGAACA TTTATATTCC 961 CCAGAACATC AGTATGCGTT TTGGATGTCA TTTCGCGTGC TGAATCAGCA CTCTCCCGAT 1021 ACGAACGGCA CTGGCATATC GTTGCATCAT GCCCAGCTAC ATCCAGATTG ACAACGGTAT 1081 GTCACGAACT TACTGAACGG CGACG // |
Primer: EYFP-N Sequence file: RDB12293_A3Jmc.seq ![]() |
>06509_12293_A3Jm_Cm_C_F_A01_01_ABI08.ab1 1 CAACGTGAAA TGGATCGGCT TACTAAAAGC CAGATAACAG TATGCGTATT TGCGCGCTGA 61 TTTTTGCGGT ATAAGAATAT ATACTGATAT GTATACCCGA AGTATGTCAA AAAGAGGTAT 121 GCTATGAAGC AGCGTATTAC AGTGACAGTT GACAGCGACA GCTATCAGTT GCTCAAGGCA 181 TATATGATGT CAATATCTCC GGTCTGGTAA GCACAACCAT GCAGAATGAA GCCCGTCGTC 241 TGCGTGCCGA ACGCTGGAAA GCGGAAAATC AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA 301 TTGAAATGAA CGGCTCTTTT GCTGACGAGA ACAGGGGCTG GTGAAATGCA GTTTAAGGTT 361 TACACCTATA AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG TGATATTATT 421 GACACGCCCG GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT GTCAGATAAA 481 GTCTCCCGTG AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG CATGATGACC 541 ACCGATATGG CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA TCTCAGCCAC 601 CGCGAAAATG ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA AATGTCAGGC 661 TCCCTTATAC ACAGCCAGTC TGCAGGTCGA CCATAGTGAC TGGATATGTT GTGTTTTACA 721 GTATTATGTA GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT 781 TTACGTTTCT CGTTCAGCTT TCTTGTACAA AGTGGTTGAT ATCATGAGTA AAGGAGAAAG 841 AACTTTTCAC TGGGAGTTTG TCCCAAATTC TTTGTTGAA // |
Primer: f1ori_F Sequence file: RDB12293_A3Jmd.seq ![]() |
>06509_12293_A3Jm_f1ori_F_B01_02_ABI08.ab1 1 GGGGGGCGCC TACGCGGCTG GCAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC 61 CGCGCTTAAT GCGCCGCTAC AGGGCGCGTA CTATGGTTGC TTTGACGTAT GCGGTGTGAA 121 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG 181 CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA 241 GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT 301 TGTAAAACGA CGGCCAGTGA ATTCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG 361 ATGATTTACA AGGTTTTGCA TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA 421 AGTTTACTAA AATTTACATA CAAATGGATG ATCTCTTCTC CATTGTTTCC TTTCTTCTTG 481 CAAACGCGTT TTACAAAGAG ATGACATGCT ATTTTCTGAT TATTTTTTTC TATAGTTTTC 541 TATTTTGTAC GTCCTTGGGA GTCCGAAATG TAAAATCGGG GTATGGTTAG TAGCGGCAGC 601 GTTCATTATG GTATATAAAA GATAAAAAGT AGGGGCAAGC GAGACAGAAT GGGATACAAG 661 GGCATCGTCT ATAATTATAG CTAAAAATTG TATTTTAATT TGTATTTTTT GTAATTTTAT 721 TTCTCATTGT TTTACTTAAA ATGAATGCGA ATTAGAGAAA ACTTCAACGA AATGTCAAAA 781 TAAGCTCAGC AAAATATACA ATTTTAGGGA AAGCGATCAG CAACTTATTC TCCGTTGTAG 841 ATTCTTTTTC TTCATAAAAT TCCAACAATG TAAAGTGCCA ATTGATTTTT CTCTAGATGC 901 TAATAAATTG AAACTAATGA AATATAATAA CACAGGTTTT GATAAATTGT AAAACTCTTA 961 CTCAAAAAAA GAATGTTAAA ACTAAATTTA ACCTTTTTAT AAAGGCTCAT GGGCGTTTAA 1021 GAGGAATAGT AAAAAATTCT ACAATTTTTT AATTTTAATG TCACCAATTG TCCTGCCGCA 1081 TATTATTCCT ATTTAATTTT CGCAACTGCC TGGTTTTAGT AACTATTACT CATATGCCTA 1141 AATGCACCTT AGTTCATGAA AGGAATGGCA AATTATTGGT C // |
Primer: LEU2pro_F Sequence file: RDB12293_A3Jme.seq ![]() |
>06509_12293_A3Jm_LEU2pro_F_C01_03_ABI08.ab1 1 CAACAATTGA GTGACAATAT TATTTAAGGA CCTATTGTTT TTTCCAATAG GTGGTTAGCA 61 ATCGTCTTAC TTTCTAACTT TTCTTACCTT TTACATTTCA GCAATATATA TATATATTTC 121 AAGGATATAC CATTCTAATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG 181 TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT 241 CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG 301 GTGTTCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGCTGATGCC GTTTTGTTAG 361 GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA 421 AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT 481 CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG 541 TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG 601 TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG 661 CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAAGCTA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
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