Resource data sheet
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pGFH1-ccdB2

S. pombe 3 ter end GFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06509
Resource name pGFH1-ccdB2
Clone info. S. pombe 3 ter end GFP-FLAG-His6-tagged vector with nmt1 promoter.
Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06509 pGFH1-ccdB2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06509z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12293_A3Jm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12293_A3Jma.seq
>06509_12293_A3Jm_nmt1Fw_A03_03.ab1
    1 CGAAATTTAA TTAAATCCAG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
   61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
  121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
  181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT
  241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT
  301 GGATCCGGAT ATCAACAAGT TTGTACAAAA AAGCTGAACG AGAAACGTAA AATGATATAA
  361 ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC
  421 AACATATCCA GTCACTATGG CGGCCGCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC
  481 CGGCTCGTAT AATGTGTGGA TTTTGAGTTA GGATCCGTCG AGATTTTCAG GAGCTAAGGA
  541 AGCTAAAATG GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG
  601 TAAAGAACAT TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA
  661 GCTGGATATT ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC
  721 CTTTATTCAC ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTCCGTA TGGCAATGAA
  781 AGACGGTGAG CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA
  841 AACTGAAACG TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA
  901 CATATATTCG CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT
  961 TATTGAGAAT ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT
 1021 AAACGTGGCC CAATATGGAC AACTTTCTTT CGCCCCCGTT TTCACCATGG GGCAAATATA
 1081 TACGCAAGGG CGACAGGTTG CCTGAATGCG CTGCCGAATC CAGTCCATTC ATGCCGATTT
1141 GTTGAATGGC CATCCAATGG TATCCCG
//
Primer: nmt1Rv | Sequence: RDB12293_A3Jmb.seq
>06509_12293_A3Jm_nmt1Rv_B03_06.ab1
    1 ATCCGCGGTC TCGAGATCTC AGTGGTGATG GTGGTGATGT TTGTCATCGT CGTCCTTGTA
   61 GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC
  121 AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA
  181 CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA
  241 ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT
  301 GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC
  361 CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT
  421 ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC
  481 TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC
  541 ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA ACACCATAAC AGAAAGTAGT
  601 GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG CAAATAAATT TAAGGGTAAG
  661 TTTTCCGTAT GTTGCATCAC CTTCACCCTC TCCACTGACA GAAAATTTGT GCCCATTAAC
  721 ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG AAAAGTTCTT CTCCTTTACT
  781 CATGATATCA ACCACTTTGT ACAGAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
  841 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA TACTGTAAAA CACACATATC
  901 CAGTCCACTA TGGTCGACCT GCAGACTGGC TGTGTATAAG AGCCTGAACA TTTATATTCC
  961 CCAGAACATC AGTATGCGTT TTGGATGTCA TTTCGCGTGC TGAATCAGCA CTCTCCCGAT
 1021 ACGAACGGCA CTGGCATATC GTTGCATCAT GCCCAGCTAC ATCCAGATTG ACAACGGTAT
1081 GTCACGAACT TACTGAACGG CGACG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

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