Resource data sheet
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pYFH1-ccdB2 rfA

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06505
Resource name pYFH1-ccdB2 rfA
Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter.
Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06505 pYFH1-ccdB2 rfA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06505z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12332_A3Jm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB12332_A3Jma.seq
>06505_12332_A3Jm_nmt1Fw_A02_02.ab1
    1 GGGAAATTTT ATTTAATCAG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
   61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
  121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
  181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT
  241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT
  301 GGATCCGGAT ATCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA
  361 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA
  421 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC
  481 GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA
  541 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT
  601 AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG
661 CTGGATATTA CGGCC
//
Primer: nmt1Rv | Sequence: RDB12332_A3Jmb.seq
>06505_12332_A3Jm_nmt1Rv_B02_05.ab1
    1 AGCGCCGGGG GCTCGAGATC TCAGTGGTGA TGGTGGTGAT GTTTGTCATC GTCGTCCTTG
   61 TAGTCCATGG GCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC
  121 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGTAG
  181 CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG
  241 TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTCACC
  301 TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT GTAGTTGTAC
  361 TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG
  421 CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG
  481 TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG
  541 TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCAGGCCGTA GCCGAAGGTG
  601 GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC
661 AGCTTGCCGT AG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

GNP_filter3_RDBDEP_html_190927.pl