RDB06505 - pYFH1-ccdB2 rfA

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pYFH1-ccdB2 rfA (#RDB06505)

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Clone info.	S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter.
Comment	Gateway compatible.
Vector backbone	pREP1 (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06505z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06505	pYFH1-ccdB2 rfA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYFH1-ccdB2 rfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06505).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12332_A3Jm.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB12332_A3Jma.seq
>06505_12332_A3Jm_nmt1Fw_A02_02.ab1 1 GGGAAATTTT ATTTAATCAG GGAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC 61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT 121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG 181 ATATATAAAG GAAGAGGAAT CCTGGCATAT CATCAATTGA ATAAGTTGAA TTAATTATTT 241 CAATCTCATT CTCACTTTCT GACTTATAGT CGCTTTGTTA AAGCTAGCGT CGACAGGCCT 301 GGATCCGGAT ATCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA 361 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA 421 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 481 GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA 541 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT 601 AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG 661 CTGGATATTA CGGCC //
Primer: nmt1Rv Sequence file: RDB12332_A3Jmb.seq
>06505_12332_A3Jm_nmt1Rv_B02_05.ab1 1 AGCGCCGGGG GCTCGAGATC TCAGTGGTGA TGGTGGTGAT GTTTGTCATC GTCGTCCTTG 61 TAGTCCATGG GCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC 121 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGTAG 181 CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG 241 TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTCACC 301 TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT GTAGTTGTAC 361 TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG 421 CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG 481 TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG 541 TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT GCAGGCCGTA GCCGAAGGTG 601 GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC 661 AGCTTGCCGT AG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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Reference sequence