Resource data sheet
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pDUAL-HFG21c

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with tub1 promoter

Catalog number RDB06493
Resource name pDUAL-HFG21c
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with tub1 promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Gateway compatible.
Vector backbone pDUAL (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of nucleotide sequence AB364139.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering Please visit Information of Request for Distribution. 
Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06493 pDUAL-HFG21c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06493z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12324_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: | Sequence: RDB12324_A3Kla.seq
>RDB12324_Cm_N_rev_H11_23.ab1
    1 CATTCTCAAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT
   61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GACGGATCCT AACTCAAAAT
  121 CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGCGGCCGC
  181 CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC
  241 TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA
  301 CTTGTGATAT CTTTGTATAG TTCATCCATG CCATGTGTAA TCCCAGCAGC TGTTACAAAC
  361 TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT TCGAAAGGGC AGATTGTGTG
  421 GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC CAATTGGAGT ATTTTGTTGA
  481 TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT GTCTAATTTT GAAGTTAACT
  541 TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT TGTGTGAGTT ATAGTTGTAT
  601 TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT CAATACCTTT TAACTCGATT
661 CTATTAACAA GGGT
//
Primer: | Sequence: RDB12324_A3Klb.seq
>RDB12324_ADH_rev_A12_03.ab1
    1 TAAAGGGTTT CTCCAAGAAT AAGAATTTTC GTTTTAAAAC CTAAGAGTCA CTTTAAAATT
   61 TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT CATAAGAAAT
  121 TCGCTTATTT AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCATG ATATCACCAC
  181 TTTGTACAAG AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA
  241 GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG
  301 GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA
  361 GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA
  421 TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA
  481 TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA
  541 GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA
  601 GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG
661 TTCTCGTCAG CAAAAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

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