S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter
| Catalog number | RDB06467 |
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| Resource name | pLYS1U-GFH21c |
| Clone info. | S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter. Gateway compatible. |
| Vector backbone | pUC119 (Plasmid) |
| Selectable markers | E. coli: Amp^r, Cm^r, ccdB. |
| Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Use Survival2 (Invitrogen) or equivalent to amplify this clone. Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
|---|---|---|---|---|
| RDB06467 | pLYS1U-GFH21c | Under QC test. Please contact us. | DNA solution |
Electronic file
| Electronic file | Sequence RDB06467z.seq from Depositor |
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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This clone will be sequenced a portion and digested by restriction enzyme for examination.
Original, user report and related articles
| user report | Ito, D., Pericentrin-mediated SAS-6 recruitment promotes centriole assembly. Elife 8. pii: e41418 (2019). PMID 31182187. |
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2019.10.05
GNP_filter3_RDBDEP_html_190927.pl