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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pLYS1U-CFH21c

S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter

Catalog number RDB06463
Resource name pLYS1U-CFH21c
Clone info. S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter.
Comment Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06463 pLYS1U-CFH21c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06463z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12321_A3Kl.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer:
            Sequence file: RDB12321_A3Kla.seq check
            >RDB12321_Cm_N_rev_C10_07.ab1
                1 CTATCTGAAT GTTCTTTACG ATGCCATTGG GATATATCAG ACGGTGGTAT ATCCAGTGAT
               61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GACGGATCCT AACTCAAAAT
              121 CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGCGGCCGC
              181 CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC
              241 TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA
              301 CTTGTTGATA TCCGGATCCA GGCCTGTCGA CGCTAGCGAA CGTCTTTCCA GGGTTTCTAC
              361 GAGGTTTTTG GTGTTTGGTT TGTAGTGAAG TGCGGTGTAC ACTTAATCAA AACAATTCCT
              421 TAATAAAGAA GTCTAACGAT ATTTATTGAT GATACGGAAG GAAGTATGCT AAACATGTAA
              481 ATAGACATTA AAATAATGCA TTGCTTGCAA AAAGAAGCTT ACTCAGTCAG GTGTTTTAAG
              541 CTTTCTAATC ATAAAAAGAT TAGGATCTTC ATCAATTTCA CCTTTTATTT TCTCAATTTG
              601 TAAGTGAAAG ATTGGATTGG TAAATTTCCA TTAAAACTCA ACATGTGCTC CTTTATAGAA
            661 CAGGTATATC CTC
            //
            Primer:
            Sequence file: RDB12321_A3Klb.seq check
            >RDB12321_EGFP_N_D10_10.ab1
                1 GACCCGGGGG GACGCTCCTC GCCCTTGCTC ACCATGATAT CAACCACTTT GTACAAGAAA
               61 GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA
              121 AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGTC GACCTGCAGA
              181 CTGGCTGTGT ATAAGGGAGC CTGACATTTA TATTCCCCAG AACATCAGGT TAATGGCGTT
              241 TTTGATGTCA TTTTCGCGGT GGCTGAGATC AGCCACTTCT TCCCCGATAA CGGAGACCGG
              301 CACACTGGCC ATATCGGTGG TCATCATGCG CCAGCTTTCA TCCCCGATAT GCACCACCGG
              361 GTAAAGTTCA CGGGAGACTT TATCTGACAG CAGACGTGCA CTGGCCAGGG GGATCACCAT
              421 CCGTCGCCCG GGCGTGTCAA TAATATCACT CTGTACATCC ACAAACAGAC GATAACGGCT
              481 CTCTCTTTTA TAGGTGTAAA CCTTAAACTG CATTTCACCA GCCCCTGTTC TCGTCAGCAA
              541 AAGAGCCGTT CATTTCAATA AACCGGGCGA CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT
              601 TCCAGCGTTC GGCACGCAGA CGACGGGCTT CATTCTGCAT GGTTGTGCTT ACCAGACCGG
            661 AGATATTGAC ATCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl