Resource data sheet
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pLYS1U-CFH21c

S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter

Catalog number RDB06463
Resource name pLYS1U-CFH21c
Clone info. S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter.
Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Order form 
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06463 pLYS1U-CFH21c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06463z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12321_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: | Sequence: RDB12321_A3Kla.seq
>RDB12321_Cm_N_rev_C10_07.ab1
    1 CTATCTGAAT GTTCTTTACG ATGCCATTGG GATATATCAG ACGGTGGTAT ATCCAGTGAT
   61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GACGGATCCT AACTCAAAAT
  121 CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGCGGCCGC
  181 CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC
  241 TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA
  301 CTTGTTGATA TCCGGATCCA GGCCTGTCGA CGCTAGCGAA CGTCTTTCCA GGGTTTCTAC
  361 GAGGTTTTTG GTGTTTGGTT TGTAGTGAAG TGCGGTGTAC ACTTAATCAA AACAATTCCT
  421 TAATAAAGAA GTCTAACGAT ATTTATTGAT GATACGGAAG GAAGTATGCT AAACATGTAA
  481 ATAGACATTA AAATAATGCA TTGCTTGCAA AAAGAAGCTT ACTCAGTCAG GTGTTTTAAG
  541 CTTTCTAATC ATAAAAAGAT TAGGATCTTC ATCAATTTCA CCTTTTATTT TCTCAATTTG
  601 TAAGTGAAAG ATTGGATTGG TAAATTTCCA TTAAAACTCA ACATGTGCTC CTTTATAGAA
661 CAGGTATATC CTC
//
Primer: | Sequence: RDB12321_A3Klb.seq
>RDB12321_EGFP_N_D10_10.ab1
    1 GACCCGGGGG GACGCTCCTC GCCCTTGCTC ACCATGATAT CAACCACTTT GTACAAGAAA
   61 GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA
  121 AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGTC GACCTGCAGA
  181 CTGGCTGTGT ATAAGGGAGC CTGACATTTA TATTCCCCAG AACATCAGGT TAATGGCGTT
  241 TTTGATGTCA TTTTCGCGGT GGCTGAGATC AGCCACTTCT TCCCCGATAA CGGAGACCGG
  301 CACACTGGCC ATATCGGTGG TCATCATGCG CCAGCTTTCA TCCCCGATAT GCACCACCGG
  361 GTAAAGTTCA CGGGAGACTT TATCTGACAG CAGACGTGCA CTGGCCAGGG GGATCACCAT
  421 CCGTCGCCCG GGCGTGTCAA TAATATCACT CTGTACATCC ACAAACAGAC GATAACGGCT
  481 CTCTCTTTTA TAGGTGTAAA CCTTAAACTG CATTTCACCA GCCCCTGTTC TCGTCAGCAA
  541 AAGAGCCGTT CATTTCAATA AACCGGGCGA CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT
  601 TCCAGCGTTC GGCACGCAGA CGACGGGCTT CATTCTGCAT GGTTGTGCTT ACCAGACCGG
661 AGATATTGAC ATCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

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