Please review the QC test results indicated by icon below as well as clone information before placing your order.

pLYS1K-FFH1c

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12267_A3J7.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB12267_A3J7a.seq
>06458_12267_A3J7_nmt1Fw_B08_05.ab1     1 AGGAAATTTA TTAAATCCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA    61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT   121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA   181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC   241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG   301 GATCCGGATA TCAACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA   361 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA   421 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC   481 GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA   541 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT   601 AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG 661 CTGGATATTA CGGCC //
Primer: Sequence file: RDB12267_A3J7b.seq
>06458_12267_A3J7_ADHrev_C08_08.ab1     1 GGAGGGGTCC TCAAGATAAG AATTTTCGTT TTAAAACCTA AGAGTCACTT TAAAATTTTG    61 TATACACTTA TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC   121 GCTTATTTAG AAGTGGCGCG CCGGATCCTC TAGAGTCGAG ATCTCAGTGG TGATGGTGGT   181 GATGTTTGTC ATCGTCGTCC TTGTAGTCCA TGGGTTTATC GTCATCATCC TTATAATCCA   241 TGATATCAAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA   301 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA   361 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT   421 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC   481 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG   541 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA   601 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT 661 ACATCCACAA ACAGA //
Primer: Sequence file: RDB12267_A3J7c.seq
>06458_12267_A3J7_nmt1Fw2_D08_11.ab1     1 GTCATGGGCG ACGATGACAA CATCACCACC ATCACCACTG AGATCTCGAC TCTAGAGGAT    61 CCGGCGCGCC ACTTCTAAAT AAGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA   121 AGTTATAAAA AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA   181 ATTCTTATTC TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGTATGA   241 GGTCGCTCTT ATTGACCACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA   301 CTAGTACTTT GTAGTACCTG CGCGGTCTCA AGATCAAACA TTTTTGGAAT CGCAAAAAGA   361 TGTCATTCCA GCCGGACGTG GCATGAAGAA TGTCCAACTT TTGGTGATCA ATCGTTTTGA   421 TACTAACAAG ATATGCGGTA TCGGAGAAGT TGGTGAAATA TATCTTCGTG CTGGTGGATT   481 GGCTGAGGGC TACCTTGGAA ATGATGAATT GACTAGCAAA AAATTCTTAA AGAGTTGGTT   541 TGCTGATCCC TCCAAGTTTG TAGACCGAAC TCCAGAAAAT GCGCCCTGGA AACCTTATTG   601 GTTCGGAATA CGCGATCGTA TGTATCGTTC TGGTGACTTG GGCCGCTATC TCCCAACTGG 661 TAATGTAGAA TGTAGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl