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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pLYS1K-FFH1c (#RDB06458)

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06458z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06458 pLYS1K-FFH1c DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pLYS1K-FFH1c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06458).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12267_A3J7.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12267_A3J7a.seq check
>06458_12267_A3J7_nmt1Fw_B08_05.ab1
    1 AGGAAATTTA TTAAATCCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
  241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
  301 GATCCGGATA TCAACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA
  361 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA
  421 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC
  481 GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA
  541 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT
  601 AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG
661 CTGGATATTA CGGCC
//
Primer:
Sequence file: RDB12267_A3J7b.seq check
>06458_12267_A3J7_ADHrev_C08_08.ab1
    1 GGAGGGGTCC TCAAGATAAG AATTTTCGTT TTAAAACCTA AGAGTCACTT TAAAATTTTG
   61 TATACACTTA TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC
  121 GCTTATTTAG AAGTGGCGCG CCGGATCCTC TAGAGTCGAG ATCTCAGTGG TGATGGTGGT
  181 GATGTTTGTC ATCGTCGTCC TTGTAGTCCA TGGGTTTATC GTCATCATCC TTATAATCCA
  241 TGATATCAAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
  301 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA
  361 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT
  421 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC
  481 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG
  541 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA
  601 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT
661 ACATCCACAA ACAGA
//
Primer:
Sequence file: RDB12267_A3J7c.seq check
>06458_12267_A3J7_nmt1Fw2_D08_11.ab1
    1 GTCATGGGCG ACGATGACAA CATCACCACC ATCACCACTG AGATCTCGAC TCTAGAGGAT
   61 CCGGCGCGCC ACTTCTAAAT AAGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA
  121 AGTTATAAAA AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA
  181 ATTCTTATTC TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGTATGA
  241 GGTCGCTCTT ATTGACCACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
  301 CTAGTACTTT GTAGTACCTG CGCGGTCTCA AGATCAAACA TTTTTGGAAT CGCAAAAAGA
  361 TGTCATTCCA GCCGGACGTG GCATGAAGAA TGTCCAACTT TTGGTGATCA ATCGTTTTGA
  421 TACTAACAAG ATATGCGGTA TCGGAGAAGT TGGTGAAATA TATCTTCGTG CTGGTGGATT
  481 GGCTGAGGGC TACCTTGGAA ATGATGAATT GACTAGCAAA AAATTCTTAA AGAGTTGGTT
  541 TGCTGATCCC TCCAAGTTTG TAGACCGAAC TCCAGAAAAT GCGCCCTGGA AACCTTATTG
  601 GTTCGGAATA CGCGATCGTA TGTATCGTTC TGGTGACTTG GGCCGCTATC TCCCAACTGG
661 TAATGTAGAA TGTAGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles