Resource data sheet
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pLYS1K-FFH1c

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06458
Resource name pLYS1K-FFH1c
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Distribution information (domestic users) [open/close]


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RDB06458 pLYS1K-FFH1c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06458z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12267_A3J7.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: | Sequence: RDB12267_A3J7a.seq
>06458_12267_A3J7_nmt1Fw_B08_05.ab1
    1 AGGAAATTTA TTAAATCCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
  241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
  301 GATCCGGATA TCAACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA
  361 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA
  421 ACATATCCAG TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC
  481 GGCTCGTATA ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA
  541 GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT
  601 AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG
661 CTGGATATTA CGGCC
//
Primer: | Sequence: RDB12267_A3J7b.seq
>06458_12267_A3J7_ADHrev_C08_08.ab1
    1 GGAGGGGTCC TCAAGATAAG AATTTTCGTT TTAAAACCTA AGAGTCACTT TAAAATTTTG
   61 TATACACTTA TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC
  121 GCTTATTTAG AAGTGGCGCG CCGGATCCTC TAGAGTCGAG ATCTCAGTGG TGATGGTGGT
  181 GATGTTTGTC ATCGTCGTCC TTGTAGTCCA TGGGTTTATC GTCATCATCC TTATAATCCA
  241 TGATATCAAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
  301 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA
  361 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT
  421 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC
  481 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG
  541 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA
  601 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT
661 ACATCCACAA ACAGA
//
Primer: | Sequence: RDB12267_A3J7c.seq
>06458_12267_A3J7_nmt1Fw2_D08_11.ab1
    1 GTCATGGGCG ACGATGACAA CATCACCACC ATCACCACTG AGATCTCGAC TCTAGAGGAT
   61 CCGGCGCGCC ACTTCTAAAT AAGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA
  121 AGTTATAAAA AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA
  181 ATTCTTATTC TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGTATGA
  241 GGTCGCTCTT ATTGACCACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
  301 CTAGTACTTT GTAGTACCTG CGCGGTCTCA AGATCAAACA TTTTTGGAAT CGCAAAAAGA
  361 TGTCATTCCA GCCGGACGTG GCATGAAGAA TGTCCAACTT TTGGTGATCA ATCGTTTTGA
  421 TACTAACAAG ATATGCGGTA TCGGAGAAGT TGGTGAAATA TATCTTCGTG CTGGTGGATT
  481 GGCTGAGGGC TACCTTGGAA ATGATGAATT GACTAGCAAA AAATTCTTAA AGAGTTGGTT
  541 TGCTGATCCC TCCAAGTTTG TAGACCGAAC TCCAGAAAAT GCGCCCTGGA AACCTTATTG
  601 GTTCGGAATA CGCGATCGTA TGTATCGTTC TGGTGACTTG GGCCGCTATC TCCCAACTGG
661 TAATGTAGAA TGTAGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

GNP_filter3_RDBDEP_html_190927.pl