Resource data sheet
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pHIS3K-GFH21c

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter

Catalog number RDB06449
Resource name pHIS3K-GFH21c
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter.
Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06449 pHIS3K-GFH21c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06449z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12320_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: | Sequence: RDB12320_A3Kla.seq
>RDB12320_Cm_N_rev_A10_01.ab1
    1 CTGTCCTAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT
   61 TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ACGGATCCTA ACTCAAAATC
  121 CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC
  181 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT
  241 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTT TTGTACAAAC
  301 TTGTTGATAT CCGGATCCAG GCCTGTCGAC GCTAGCGAAC GTCTTTCCAG GGTTTCTACG
  361 AGGTTTTTGG TGTTTGGTTT GTAGTGAAGT GCGGTGTACA CTTAATCAAA ACAATTCCTT
  421 AATAAAGAAG TCTAACGATA TTTATTGATG ATACGGAAGG AAGTATGCTA AACATGTAAA
  481 TAGACATTAA AATAATGCAT TGCTTGCAAA AAGAAGCTTA CTCAGTCAGG TGTTTTAAGC
  541 TTTCTAATCA TAAAAAGATT AGGATCTTCA TCAATTTCAC CTTTTATTTT CTCAATTTGT
  601 AAGTGAAAGA TTGGATTGGT AAATTTCCAT TAAAACTCAA CATGTGCTCC TTTATAGAAC
661 AGGTATATCC TC
//
Primer: | Sequence: RDB12320_A3Klb.seq
>RDB12320_yEGFP_N_B10_04.ab1
    1 ATCAGGTCTT GGCACGGACA GGTAGTTTTC CAGTAGTGCA AATAAATTTA AGGGTAAGTT
   61 TTCCGTATGT TGCATCACCT TCACCCTCTC CACTGACAGA AAATTTGTGC CCATTAACAT
  121 CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA AAGTTCTTCT CCTTTACTCA
  181 TGATATCAAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
  241 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA
  301 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT
  361 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC
  421 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG
  481 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA
  541 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT
  601 ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT
661 TCACCAGCCC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.09.27

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