RDB06449 - pHIS3K-GFH21c

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pHIS3K-GFH21c (#RDB06449)

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter

Clone info.	S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter.
Comment	Gateway compatible.
Vector backbone	pUC119 (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06449z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06449	pHIS3K-GFH21c	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHIS3K-GFH21c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06449).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12320_A3Kl.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB12320_A3Kla.seq
>RDB12320_Cm_N_rev_A10_01.ab1 1 CTGTCCTAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 61 TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ACGGATCCTA ACTCAAAATC 121 CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC 181 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT 241 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTT TTGTACAAAC 301 TTGTTGATAT CCGGATCCAG GCCTGTCGAC GCTAGCGAAC GTCTTTCCAG GGTTTCTACG 361 AGGTTTTTGG TGTTTGGTTT GTAGTGAAGT GCGGTGTACA CTTAATCAAA ACAATTCCTT 421 AATAAAGAAG TCTAACGATA TTTATTGATG ATACGGAAGG AAGTATGCTA AACATGTAAA 481 TAGACATTAA AATAATGCAT TGCTTGCAAA AAGAAGCTTA CTCAGTCAGG TGTTTTAAGC 541 TTTCTAATCA TAAAAAGATT AGGATCTTCA TCAATTTCAC CTTTTATTTT CTCAATTTGT 601 AAGTGAAAGA TTGGATTGGT AAATTTCCAT TAAAACTCAA CATGTGCTCC TTTATAGAAC 661 AGGTATATCC TC //
Primer: Sequence file: RDB12320_A3Klb.seq
>RDB12320_yEGFP_N_B10_04.ab1 1 ATCAGGTCTT GGCACGGACA GGTAGTTTTC CAGTAGTGCA AATAAATTTA AGGGTAAGTT 61 TTCCGTATGT TGCATCACCT TCACCCTCTC CACTGACAGA AAATTTGTGC CCATTAACAT 121 CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA AAGTTCTTCT CCTTTACTCA 181 TGATATCAAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA 241 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA 301 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT 361 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC 421 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG 481 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA 541 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT 601 ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT 661 TCACCAGCCC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

External Database

Reference sequence