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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHIS3K-GFH21c

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter

Catalog number RDB06449
Resource name pHIS3K-GFH21c
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with tub1 promoter.
Comment Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06449 pHIS3K-GFH21c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06449z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12320_A3Kl.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer:
            Sequence file: RDB12320_A3Kla.seq check
            >RDB12320_Cm_N_rev_A10_01.ab1
                1 CTGTCCTAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT
               61 TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ACGGATCCTA ACTCAAAATC
              121 CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGCGGCCGCC
              181 ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT
              241 AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTT TTGTACAAAC
              301 TTGTTGATAT CCGGATCCAG GCCTGTCGAC GCTAGCGAAC GTCTTTCCAG GGTTTCTACG
              361 AGGTTTTTGG TGTTTGGTTT GTAGTGAAGT GCGGTGTACA CTTAATCAAA ACAATTCCTT
              421 AATAAAGAAG TCTAACGATA TTTATTGATG ATACGGAAGG AAGTATGCTA AACATGTAAA
              481 TAGACATTAA AATAATGCAT TGCTTGCAAA AAGAAGCTTA CTCAGTCAGG TGTTTTAAGC
              541 TTTCTAATCA TAAAAAGATT AGGATCTTCA TCAATTTCAC CTTTTATTTT CTCAATTTGT
              601 AAGTGAAAGA TTGGATTGGT AAATTTCCAT TAAAACTCAA CATGTGCTCC TTTATAGAAC
            661 AGGTATATCC TC
            //
            Primer:
            Sequence file: RDB12320_A3Klb.seq check
            >RDB12320_yEGFP_N_B10_04.ab1
                1 ATCAGGTCTT GGCACGGACA GGTAGTTTTC CAGTAGTGCA AATAAATTTA AGGGTAAGTT
               61 TTCCGTATGT TGCATCACCT TCACCCTCTC CACTGACAGA AAATTTGTGC CCATTAACAT
              121 CACCATCTAA TTCAACAAGA ATTGGGACAA CTCCAGTGAA AAGTTCTTCT CCTTTACTCA
              181 TGATATCAAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
              241 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA
              301 TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA GGGAGCCTGA CATTTATATT
              361 CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT CGCGGTGGCT GAGATCAGCC
              421 ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT CGGTGGTCAT CATGCGCCAG
              481 CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG AGACTTTATC TGACAGCAGA
              541 CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG TGTCAATAAT ATCACTCTGT
              601 ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG TGTAAACCTT AAACTGCATT
            661 TCACCAGCCC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

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