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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHIS3K-CFH21c

S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter

Catalog number RDB06446
Resource name pHIS3K-CFH21c
Clone info. S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter.
Comment Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06446 pHIS3K-CFH21c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06446z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17486_A9I5p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Cm_M_R
            Region: CmR,lac UV5pro,attR1
            Sequence file: RDB17486_A9I5a.seq check
            >06446_17486_A9I5_3_Cm-M_R_G10_19_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NTNNCCNGCT NNCCGTCTTT NNTTGCCNTA CGGAATTCCG
               61 GATGAGCATT CATCNNGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
              121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
              181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
              241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
              301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
              361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
              421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
              481 TTCAGCTTTT TTGTACAAAC TTGTTGATAT CCGGATCCAG GCCTGTCGAC GCTAGCGAAC
              541 GTCTTTCCAG GGTTTCTACG AGGTTTTTGG TGTTTGGTTT GTAGTGAAGT GCGGTGTACA
              601 CTTAATCAAA ACAATTCCTT AATAAAGAAG TCTAACGATA TTTATTGATG ATACGGAAGG
              661 AAGTATGCTA AACATGTAAA TAGACATTAA AATAATGCAT TGCTTGCAAA AAGAAGCTTA
              721 CTCAGTCAGG TGTTTTAAGC TTTCTAATCA TAAAAAGATT ANGATCTTCA TCAATTTCAC
              781 CTTTTATTTT CTCAATTTGT AAGTGAAAGA TTGGATTGGT AAATTTCCAT TAAAACTCAA
              841 CATGTGCTCC TTNATAGAAC AGGTATATCC TCATGTAAAT CTTTTTAAAA GTATATAAAA
            901 TATTACATN
            //
            Primer: ccdB-C_F
            Region: ccdB,attR2,ECFP
            Sequence file: RDB17486_A9I5b.seq check
            >06446_17486_A9I5_3_ccdB-C_F_A11_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTCNN GGNNGNANGN TGGCGCATGA TGACCACCGA TATGGCCAGT
               61 GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC
              121 AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC
              181 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG
              241 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC
              301 AGCTTTCTTG TACAAAGTGG TTGATATCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
              361 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC
              421 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC
              481 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG
              541 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
              601 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC
              661 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT
              721 CAAGGANGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACATCA GCCACNACGT
              781 CTATATCACC GCCNACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGCCACAA
            841 CATCGANGAN GG
            //
            Primer: EGFP-N_F
            Region: ECFP,FLAG,6xHis,ADH1ter
            Sequence file: RDB17486_A9I5c.seq check
            >06446_17486_A9I5_3_EGFP-N_F_B11_05_ABI24.ab1
                1 NNNNNNNNNN NNNGGNNNNG GNGNNGGCGN NGCNNCCTAC GGNAAGCTGN NCCTGAAGTT
               61 CATCTGCACC ACCGGNAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTG
              121 GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
              181 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
              241 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
              301 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT
              361 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA
              421 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
              481 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
              541 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
              601 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGCCC ATGGACTACA AGGACGACGA
              661 TGACAAACAT CACCACCATC ACCACTGAGA TCTCGACTCT AGAGGATCCG GCGCGCCACT
              721 TCTAAATAAG CGAATTTCTT ATGANTTATG ATTTTTATTA TTAAATAAGT TATAAAAAAA
              781 ATAAGTGTAT ACAAATTTTA AAGTGACTCT TANGTTTTAA AACGAAAATT CTTATTCTTG
            841 ANTAACTCTT TCCTGTAGGT CANGN
            //
            Primer: Venus-C_F
            Region: FLAG,6xHis,ADH1ter
            Sequence file: RDB17486_A9I5d.seq check
            >06446_17486_A9I5_3_Venus-C_F2_H10_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGNNN NNNTCTCGGN ATGGNNGAGC TGTACNAGCC CNTGGNCTAC
               61 AAGGACGACG ATGACAAACA TCACCACCAT CACCACTGAG ATCTCGACTC TAGAGGATCC
              121 GGCGCGCCAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG
              181 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT
              241 TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGTATGAGG
              301 TCGCTCTTAT TGACCACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
              361 AGTGTTGGCA GCGATGAAAT TATTGACTCC TTAATTCGCA TTTCTTGTAT TCCTGGAAAA
              421 GATAAGATTT TGATGTGTCC ACCTTCGTAC GGAATGTATA CGGTCTCTGC AAAAATCAAC
              481 GATGTTGAGG TTGTTAAGGT TCTCCTTGAA CCAGATTTCA ATTTGAACGT CGATGCTATT
              541 TGCGAAACAC TTTCTAAAGA TAGTGCCATC AAAGTCTTTT TTGCTTGTTC CCCTGGTAAT
              601 CCAACAGCGG CCGCGGGCCC GCGGCCGCTC TTAAACTCGA GGATATTAAG AAGATTTTGG
              661 AGCACCCTAC ATGGAATGGA ATCGTTGTCG TCGATGAGGC CTATATTGAT TTCTCTGCTC
            721 CCGATATGTC TGCCTTAACT CTTGTCAATG AATATCCNAA CCT
            //
            Primer: TEFpro_F
            Region: KanMX
            Sequence file: RDB17486_A9I5e.seq check
            >06446_17486_A9I5_3_TEFpro_F_D11_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNAANN NNNNGGCTCC TCGCTGCAGA NCTGCGAGCA GGGAANCGCT
               61 CCCCTCACAG ACGCGTTGAA TTGTCCCCAC GCCGCGCCCC TGTAGAGAAA TATAAAAGGT
              121 TAGGATTTGC CACTGAGGTT CTTCTTTCAT ATACTTCCTT TTAAAATCTT GCTAGGATAC
              181 AGTTCTCACA TCACATCCGA ACATAAACAA CCATGGGTAA GGAAAAGACT CACGTTTCGA
              241 GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA
              301 ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG GAAGCCCGAT GCGCCAGAGT
              361 TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC
              421 TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG
              481 ATGATGCATG GTTACTCACC ACTGCGATCC CCGGCAAAAC AGCATTCCAG GTATTAGAAG
              541 AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC
              601 ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG
              661 CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG
              721 GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAGCT TTTGCCATTC TCACCGGATT
              781 CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA
            841 TAGGTTGTAT TGATGTTGGN
            //
            Primer: M13fwd
            Region: KanMX
            Sequence file: RDB17486_A9I5f.seq check
            >06446_17486_A9I5_3_M13fwd_E11_14_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNGNG GNGTTNGTNN CGAATCGACN GCAGTATAGC GACCAGCATT
               61 CACATACGAT TGACGCATGA TATTACTTTC TGCGCACTTA ACTTCGCATC TGGGCAGATG
              121 ATGTCGAGGC GAAAAAAAAT ATAAATCACG CTAACATTTG ATTAAAATAG AACAACTACA
              181 ATATAAAAAA ACTATACAAA TGACAAGTTC TTGAAAACAA GAATCTTTTT ATTGTCAGTA
              241 CTGATTAGAA AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT
              301 CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA CCGAGGCAGT
              361 TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC GACTCGTCCA ACATCAATAC
              421 AACCTATTAA TTTCCCCTCG TCAAAAATAA GGTTATCAAG TGAGAAATCA CCATGAGTGA
              481 CGACTGAATC CGGTGAGAAT GGCAAAAGCT TATGCATTTC TTTCCAGACT TGTTCAACAG
              541 GCCAGCCATT ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG
              601 ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA CAAACAGGAA
              661 TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC AATATTTTCA CCTGAATCAG
              721 GATATTCTTC TAATACCTGG AATGCTGTTT TGCCGGGGAT CGCAGTGGTG AGTAACCATG
              781 CATCATCAGG AGTACGGATA AAATGCTTGA TGGTCGGAAG ANGCATAAAT TCCGTCAGCC
            841 AGTTTAGTCT GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACNTT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl