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pHIS3K-CFH21c

S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter

Catalog number RDB06446
Resource name pHIS3K-CFH21c
Clone info. S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter.
Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


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RDB06446 pHIS3K-CFH21c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06446z.seq from Depositor

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17486_A9I5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CmR,lac UV5pro,attR1 | Primer: Cm_M_R | Sequence: RDB17486_A9I5a.seq
>06446_17486_A9I5_3_Cm-M_R_G10_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNCCNGCT NNCCGTCTTT NNTTGCCNTA CGGAATTCCG
   61 GATGAGCATT CATCNNGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT
  121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC
  181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA
  241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG
  301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
  361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
  421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG
  481 TTCAGCTTTT TTGTACAAAC TTGTTGATAT CCGGATCCAG GCCTGTCGAC GCTAGCGAAC
  541 GTCTTTCCAG GGTTTCTACG AGGTTTTTGG TGTTTGGTTT GTAGTGAAGT GCGGTGTACA
  601 CTTAATCAAA ACAATTCCTT AATAAAGAAG TCTAACGATA TTTATTGATG ATACGGAAGG
  661 AAGTATGCTA AACATGTAAA TAGACATTAA AATAATGCAT TGCTTGCAAA AAGAAGCTTA
  721 CTCAGTCAGG TGTTTTAAGC TTTCTAATCA TAAAAAGATT ANGATCTTCA TCAATTTCAC
  781 CTTTTATTTT CTCAATTTGT AAGTGAAAGA TTGGATTGGT AAATTTCCAT TAAAACTCAA
  841 CATGTGCTCC TTNATAGAAC AGGTATATCC TCATGTAAAT CTTTTTAAAA GTATATAAAA
901 TATTACATN
//
Region: ccdB,attR2,ECFP | Primer: ccdB-C_F | Sequence: RDB17486_A9I5b.seq
>06446_17486_A9I5_3_ccdB-C_F_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTCNN GGNNGNANGN TGGCGCATGA TGACCACCGA TATGGCCAGT
   61 GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC
  121 AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC
  181 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG
  241 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC
  301 AGCTTTCTTG TACAAAGTGG TTGATATCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
  361 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC
  421 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC
  481 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG
  541 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
  601 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC
  661 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT
  721 CAAGGANGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACATCA GCCACNACGT
  781 CTATATCACC GCCNACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGCCACAA
841 CATCGANGAN GG
//
Region: ECFP,FLAG,6xHis,ADH1ter | Primer: EGFP-N_F | Sequence: RDB17486_A9I5c.seq
>06446_17486_A9I5_3_EGFP-N_F_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNNGGNNNNG GNGNNGGCGN NGCNNCCTAC GGNAAGCTGN NCCTGAAGTT
   61 CATCTGCACC ACCGGNAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTG
  121 GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
  181 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
  241 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
  301 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT
  361 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA
  421 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
  481 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
  541 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
  601 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGCCC ATGGACTACA AGGACGACGA
  661 TGACAAACAT CACCACCATC ACCACTGAGA TCTCGACTCT AGAGGATCCG GCGCGCCACT
  721 TCTAAATAAG CGAATTTCTT ATGANTTATG ATTTTTATTA TTAAATAAGT TATAAAAAAA
  781 ATAAGTGTAT ACAAATTTTA AAGTGACTCT TANGTTTTAA AACGAAAATT CTTATTCTTG
841 ANTAACTCTT TCCTGTAGGT CANGN
//
Region: FLAG,6xHis,ADH1ter | Primer: Venus-C_F | Sequence: RDB17486_A9I5d.seq
>06446_17486_A9I5_3_Venus-C_F2_H10_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNNTCTCGGN ATGGNNGAGC TGTACNAGCC CNTGGNCTAC
   61 AAGGACGACG ATGACAAACA TCACCACCAT CACCACTGAG ATCTCGACTC TAGAGGATCC
  121 GGCGCGCCAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG
  181 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT
  241 TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGTATGAGG
  301 TCGCTCTTAT TGACCACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
  361 AGTGTTGGCA GCGATGAAAT TATTGACTCC TTAATTCGCA TTTCTTGTAT TCCTGGAAAA
  421 GATAAGATTT TGATGTGTCC ACCTTCGTAC GGAATGTATA CGGTCTCTGC AAAAATCAAC
  481 GATGTTGAGG TTGTTAAGGT TCTCCTTGAA CCAGATTTCA ATTTGAACGT CGATGCTATT
  541 TGCGAAACAC TTTCTAAAGA TAGTGCCATC AAAGTCTTTT TTGCTTGTTC CCCTGGTAAT
  601 CCAACAGCGG CCGCGGGCCC GCGGCCGCTC TTAAACTCGA GGATATTAAG AAGATTTTGG
  661 AGCACCCTAC ATGGAATGGA ATCGTTGTCG TCGATGAGGC CTATATTGAT TTCTCTGCTC
721 CCGATATGTC TGCCTTAACT CTTGTCAATG AATATCCNAA CCT
//
Region: KanMX | Primer: TEFpro_F | Sequence: RDB17486_A9I5e.seq
>06446_17486_A9I5_3_TEFpro_F_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNAANN NNNNGGCTCC TCGCTGCAGA NCTGCGAGCA GGGAANCGCT
   61 CCCCTCACAG ACGCGTTGAA TTGTCCCCAC GCCGCGCCCC TGTAGAGAAA TATAAAAGGT
  121 TAGGATTTGC CACTGAGGTT CTTCTTTCAT ATACTTCCTT TTAAAATCTT GCTAGGATAC
  181 AGTTCTCACA TCACATCCGA ACATAAACAA CCATGGGTAA GGAAAAGACT CACGTTTCGA
  241 GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA
  301 ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG GAAGCCCGAT GCGCCAGAGT
  361 TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC
  421 TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG
  481 ATGATGCATG GTTACTCACC ACTGCGATCC CCGGCAAAAC AGCATTCCAG GTATTAGAAG
  541 AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC
  601 ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG
  661 CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG
  721 GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAGCT TTTGCCATTC TCACCGGATT
  781 CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA
841 TAGGTTGTAT TGATGTTGGN
//
Region: KanMX | Primer: M13fwd | Sequence: RDB17486_A9I5f.seq
>06446_17486_A9I5_3_M13fwd_E11_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNG GNGTTNGTNN CGAATCGACN GCAGTATAGC GACCAGCATT
   61 CACATACGAT TGACGCATGA TATTACTTTC TGCGCACTTA ACTTCGCATC TGGGCAGATG
  121 ATGTCGAGGC GAAAAAAAAT ATAAATCACG CTAACATTTG ATTAAAATAG AACAACTACA
  181 ATATAAAAAA ACTATACAAA TGACAAGTTC TTGAAAACAA GAATCTTTTT ATTGTCAGTA
  241 CTGATTAGAA AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT
  301 CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA CCGAGGCAGT
  361 TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC GACTCGTCCA ACATCAATAC
  421 AACCTATTAA TTTCCCCTCG TCAAAAATAA GGTTATCAAG TGAGAAATCA CCATGAGTGA
  481 CGACTGAATC CGGTGAGAAT GGCAAAAGCT TATGCATTTC TTTCCAGACT TGTTCAACAG
  541 GCCAGCCATT ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG
  601 ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA CAAACAGGAA
  661 TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC AATATTTTCA CCTGAATCAG
  721 GATATTCTTC TAATACCTGG AATGCTGTTT TGCCGGGGAT CGCAGTGGTG AGTAACCATG
  781 CATCATCAGG AGTACGGATA AAATGCTTGA TGGTCGGAAG ANGCATAAAT TCCGTCAGCC
841 AGTTTAGTCT GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACNTT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles


2019.10.05

GNP_filter3_RDBDEP_html_190927.pl