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pGEM3Zf_hsSox2 (#RDB06430)

Plasmid clone of human Sox2 cDNA.

Alternative name SET-0215_12
Clone info. Plasmid clone of human Sox2 cDNA.
Comment Derived from IRAUp969A0546D6. PCR cloning, forward primer: atgtacaacatgatggagacgga; reverse primer: tcacatgtgtgagaggggcagt.
Vector backbone pGEM-3Zf(+) (Plasmid)
Size of vector backbone 3,197 bp
Selectable markers Amp^r
Gene/insert name Human Sox2 cDNA
Depositor|Developer DNA Bank, |
 

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06430 pGEM3Zf_hsSox2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEM3Zf_hsSox2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06430).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12330_A3Kr.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12330_A3Kra.seq check
>06430_12330_A3Kr_M13(-40)_E10_13.ab1
    1 CGGCAAGCTA ATTGTATACG ACTCACTATA GGGCGAATTC GAGCTCGGTA CCCGGGGATC
   61 CTCTAGAGTC ATGTACAACA TGATGGAGAC GGAGCTGAAG CCGCCGGGCC CGCAGCAAAC
  121 TTCGGGGGGC GGCGGCGGCA ACTCCACCGC GGCGGCGGCC GGCGGCAACC AGAAAAACAG
  181 CCCGGACCGC GTCAAGCGGC CCATGAATGC CTTCATGGTG TGGTCCCGCG GGCAGCGGCG
  241 CAAGATGGCC CAGGAGAACC CCAAGATGCA CAACTCGGAG ATCAGCAAGC GCCTGGGCGC
  301 CGAGTGGAAA CTTTTGTCGG AGACGGAGAA GCGGCCGTTC ATCGACGAGG CTAAGCGGCT
  361 GCGAGCGCTG CACATGAAGG AGCACCCGGA TTATAAATAC CGGCCCCGGC GGAAAACCAA
  421 GACGCTCATG AAGAAGGATA AGTACACGCT GCCCGGCGGG CTGCTGGCCC CCGGCGGCAA
  481 TAGCATGGCG AGCGGGGTCG GGGTGGGCGC CGGCCTGGGC GCGGGCGTGA ACCAGCGCAT
  541 GGACAGTTAC GCGCACATGA ACGGCTGGAG CAACGGCAGC TACAGCATGA TGCAGGACCA
  601 GCTGGGCTAC CCGCAGCACC CGGGCCTCAA TGCGCACGGC GCAGCGCAGA TGCAGCCCAT
661 GCACCGCTAC GACGTGAGCG CCCTGCAGTA CAACTCCATG
//
Primer:
Sequence file: RDB12330_A3Krb.seq check
>06430_12330_A3Kr_Reverse2_F10_16.ab1
    1 AATAACATGA TTACGCCAGC TATTTAGGTG ACACTATAGA ATACTCAAGC TTGCATGCCT
   61 GCAGGTCTCA CATGTGTGAG AGGGGCAGTG TGCCGTTAAT GGCCGTGCCG GGCACCGGGC
  121 CGCTCTGGTA GTGCTGGGAC ATGTGAAGTC TGCTGGGGGC GGCGGGTTCC GGCACCTCGG
  181 CGCCGGGGAG ATACATGCTG ATCATGTCCC GGAGGTCCCC GGCCTGGCAG GGCGCCCTGG
  241 AGTGGGAGGA AGAGGTAACC ACAGGGGGGC TGGAGCTGGC CTCGGACTTG ACCACCGAAC
  301 CCATGGAGCC AAGAGCCATG CCAGGGGTGC CCTGCTGCGA GTAGGACATG CTGTAGGTGG
  361 GCGAGCCGTT CATGTAGGTC TGCGAGCTGG TCATGGAGTT GTACTGCAGG GCGCTCACGT
  421 CGTAGCGGTG CATGGGCTGC ATCTGCGCTG CGCCGTGCGC ATTGAGGCCC GGGTGCTGCG
  481 GGTAGCCCAG CTGGTCCTGC ATCATGCTGT AGCTGCCGTT GCTCCAGCCG TTCATGTGCG
  541 CGTAACTGTC CATGCGCTGG TTCACGCCCG CGCCCAGGCC GGCGCCCACC CCGACCCCGC
  601 TCGCCATGCT ATTGCCGCCG GGGGCCAGCA GCCCGCCGGG CAGCGTGTAC TTATCCTTCT
661 TCATGAGCGT CTTGGTTTTC CGCCGGGGCC GGTATTTATA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles