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pCMFlag_hsCCNH (#RDB06416)

Expression vector of human CCNH.

Alternative name SET-0192_1
Clone info. Expression vector of human CCNH. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano CAS09072. PCR cloning, forward primer: atgtaccacaacagtagtcagaagc; reverse primer: ttagagagattctaccaggtcgtc.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human CCNH/Cyc H cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06416.pdf

Distribution information

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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

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RDB06416 pCMFlag_hsCCNH DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsCCNH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06416).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12340_A3Li.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12340_A3Lia.seq check
>06416_12340_A3Li_CMV-Foward_A04_01.ab1
    1 CGGTTCCACC TCCCGCCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GAATTCACCA TGGACTACAA GGACGATGAC
  181 GATAAGGCGG CCATGTACCA CAACAGTAGT CAGAAGCGGC ACTGGACCTT CTCCAGCGAG
  241 GAGCAGCTGG CAAGACTGCG GGCTGACGCC AACCGCAAAT TCAGATGCAA AGCCGTGGCC
  301 AACGGGAAGG TTCTTCCGAA TGATCCAGTC TTTCTTGAGC CTCATGAAGA AATGACACTC
  361 TGCAAATACT ATGAGAAAAG GTTATTGGAA TTCTGTTCGG TGTTTAAGCC AGCAATGCCA
  421 AGATCTGTTG TGGGTACGGC TTGTATGTAT TTCAAACGTT TTTATCTTAA TAACTCAGTA
  481 ATGGAATATC ACCCCAGGAT AATAATGCTC ACTTGTGCAT TTTTGGCCTG CAAAGTAGAT
  541 GAATTCAATG TATCTAGTCC TCAGTTTGTT GGAAACCTCC GGGAGAGTCC TCTTGGACAG
  601 GAGAAGGCAC TTGAACAGAT ACTGGAATAT GAACTACTTC TTATACAGCA ACTTAATTTC
  661 CACCTTATTG TCCACAATCC TTACAGACCA TTTGAGGGCT TCCTCATCGA CTTAAAGACC
  721 CGCTATCCCA TATTGGAGAA TCCAGAGATT TTGAGGAAAA CAGCTGATGA CTTTCTTAAT
  781 AGAATTGCAT TGACGGATGC TTACCTTTTA TACACACCTT CCCAAATTGC CCTGACTGCC
  841 ATTTTATCTA GTGCCTCCAG GGCTGGAATT ACTATGGAAA GTTATTTATC AGAGAGTCTG
  901 ATGCTGAAAG AGAACAGAAA CTTGCCTGTC ACAGTTACTA GATATAATGA AAAGCATGAG
  961 AAACTTAGTA AAGAAGTATG AACCACCCAG ATCTGAAGAA GTTGCTGTTC TGAAACAGAA
 1021 GTTGGGAGCG ATGTCATTCT GCTGAGCTTG CACTTTAACG TAATCACGAA GAAGAGAAAG
 1081 GGCTATGAAG GATGATGATT TACGTCTCAA AGGAAATCCA AACATGAGGA GAGGATGGAA
 1141 CTGATGACGA CCTGGTAGAT CTCCTCTAAG TCGCTTCGAG CATGCATTCT AGGAGGGCCC
1201 CATATTTCTC TATT
//
Primer:
Sequence file: RDB12340_A3Lib.seq check
>06416_12340_A3Li_BGHrev_B04_04.ab1
    1 GTTTGAATAT CCATTTAGGT GAACTATAGA ATAGGGCCCT CTAGATGCAT GCTCGAGCGG
   61 CCTTAGAGAG ATTCTACCAG GTCGTCATCA GTCCATTCTT CCTCCTCATG TTTGGATTTC
  121 TTTGAGACGT AATCATCATC TTCATAGCCT TTCCTCTTCT TCGTGATTAC GTTAAGTGCA
  181 AGCTCAGCAG AATGACATCG CTCCAACTTC TGTTTCAGAA CAGCAACTTC TTCAGATCTG
  241 GGTGGTTCAT ACTTCTTTAC TAAGTTTCTC ATGCTTTTCA TTATATCTAG TAACTGTGAC
  301 AGGCAAGTTC TGTTCTCTTT CAGCATCAGA CTCTCTGATA AATAACTTTC CATAGTAATT
  361 CCAGCCCTGG AGGCACTAGA TAAAATGGCA GTCAGGGCAA TTTGGGAAGG TGTGTATAAA
  421 AGGTAAGCAT CCGTCAATGC AATTCTATTA AGAAAGTCAT CAGCTGTTTT CCTCAAAATC
  481 TCTGGATTCT CCAATATGGG ATAGCGGGTC TTTAAGTCGA TGAGGAAGCC CTCAAATGGT
  541 CTGTAAGGAT TGTGGACAAT AAGGTGGAAA TTAAGTTGCT GTATAAGAAG TAGTTCATAT
  601 TCCAGTATCT GTTCAAGTGC CTTCTCCTGT CCAAGAGGAC TCTCCCGGAG GTTTCCAACA
  661 AACTGAGGAC TAGATACATT GAATTCATCT ACTTTGCAGG CCAAAAATGC ACAAGTGAGC
  721 ATTATTATCC TGGGGTGATA TTCCATTACT GAGTTATTAA GATAAAAACG TTTGAAATAC
  781 ATACAAGCCG TACCCACAAC AGATCTTGGC ATTGCTGGCT TAAACACCGA ACAGAATTCC
  841 AATAACCTTT TCTCATAGTA TTTGCAGAGT GTCATTTCTT TCATGAGGCT CAAGAAAGAC
  901 TGGATCATTC GGAAGAACCT TCCCGTTGGC CACGGCTTTG CATCTGAATT TGCGGTTGGC
  961 GTCAGCCCGC AGTCTTGCCA GCTGCTCCTC GCTGGAGAAA GGTCCAGTGC CGCTTCTGAC
 1021 TACTGTTGTG GTACATGGCC GCCTTATCGT CATCGTCCCT TGTAGTCCAT GGTGAAATTC
 1081 AGCTTGGTCT CCCTATAGTG AGTCGTATAA TTCGATAAGC CCAGTAAGGC AGTGGGTTCT
 1141 CTAGTAGGCC AGAGAAGCTC TGCTATATTA GACCTCCCAC GTACACTGCC TTCACGGCCA
1201 ATTGTGCCTG TCTCCTAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles