Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
[open/close]
Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsRARA (#RDB06414)
Expression vector of human RARA.
| Alternative name | SET-0086_1 |
|---|---|
| Clone info. | Expression vector of human RARA. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | Derived from Sugano AK130192. PCR cloning, forward primer: GCCAGCAACAGCAGCTCCTG; reverse primer: TCACGGGGAGTGGGTGGCCG. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human RAR alpha cDNA |
| Depositor|Developer | DNA Bank, | |
| Remarks, protocol and/or map (pdf) | RDB06414.pdf |
|---|
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06414 | pCMFlag_hsRARA | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsRARA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06414). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB06414_A3Ir.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
|
Primer: Sequence file: RDB06414_A3Ira.seq
|
|---|
|
>06414_06414_A3Ir_CMV-Foward_A12_03.ab1 1 TGGTTCCCCT TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA 61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA 121 TACGACTCAC TATAGGGAGA CCCAAGCTGA ATTCACCATG GACTACAAGG ACGATGACGA 181 TAAGGCGGCC GCCAGCAACA GCAGCTCCTG CCCGACACCT GGGGGCGGGC ACCTCAATGG 241 GTACCCGGTG CCTCCCTACG CCTTCTTCTT CCCCCCTATG CTGGGTGGAC TCTCCCCGCC 301 AGGCGCTCTG ACCACTCTCC AGCACCAGCT TCCAGTTAGT GGATATAGCA CACCATCCCC 361 AGCCACCATT GAGACCCAGA GCAGCAGTTC TGAAGAGATA GTGCCCAGCC CTCCCTCGCC 421 ACCCCCTCTA CCCCGCATCT ACAAGCCTTG CTTTGTCTGT CAGGACAAGT CCTCAGGCTA 481 CCACTATGGG GTCAGCGCCT GTGAGGGCTG CAAGGGCTTC TTCCGCCGCA GCATCCAGAA 541 GAACATGGTG TACACGTGTC ACCGGGACAA GAACTGCATC ATCAACAAGG TGACCCGGAA 601 CCGCTGCCAG TACTGCCGAC TGCAGAAGTG CTTTGAAGTG GGCATGTCCA AGGAGTCTGT 661 GAGAAACGAC CGA // |
|
Primer: Sequence file: RDB06414_A3Irb.seq
|
|
>06414_06414_A3Ir_Sp6_B12_06.ab1 1 CAATTTAGGC ATGCTCGAGC GGCTCACGGG GAGTGGGTGG CCGGGCTGCT TCTGTTGGAG 61 CTGGGGCTGA GGCTGGGGCT ACAGCTGCCT GGCGGGGGGG CCAGGCCACC CCCGTCCCGC 121 CCCCCACCCC CCGGCTGTCC GCTCAGAGTG TCCAGGCCCT CTGAGTTCTC CAACATTTCC 181 TGGATGAGAG GCGGCATGGA GCCCGGGATC TCCATCTTCA GCGTGATCAC CCGCTCAGCC 241 CCCTTGGCGC TGATGCTTCG CAGGTCAGTA ATCTTCATTA GCATCTTGGG GAACATGTGG 301 GGGCGGCTGG GCCTCCGCTT CCGCACGTAG ACCTTTAGCG CCTCCAGCAG CGGCTCCTGC 361 AGCATGTCCA CCCGGTCCGG CTGCTCCAGG TCCTGGCGGT CTCCGCAGAT GAGGCAGATG 421 GCGCTGAGCA GCCCCGTCTC CGCATCATCC ATCTCCAGGG GCAGCAGCTG GTTGGCGAAG 481 GCAAAGACCA GGTCGGTGAG GGGGCCGAAG CCAGCGTTGT GCATCTGGGT CCGGTTCAGG 541 GTCAGCCCGT CCGAGAAGGT CATGGTGTCC TGCTCGGGCG TGTACCGCGT GCAGATCCGC 601 AGGATCAGGA TGTCCAGGCA GGCAGCCTTG AGGAGGGTGA TCTGGTCGGC GATGGTGAGG 661 GTGGTGAAGC CGGGCA // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human RAR alpha
Reference sequence
Tips
Featured content