Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsSTAT6 (#RDB06383)

Expression vector of human STAT6.

Alternative name SET-0181_9
Clone info. Expression vector of human STAT6. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano BC075852. PCR cloning, forward primer: atgtctctgtggggtctggtctcc; reverse primer: gatcaccaactggggttggc.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human STAT6 cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06383 pCMFlag_hsSTAT6 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsSTAT6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06383).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06383_A3Ir.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB06383_A3Ira.seq check
>06383_06383_A3Ir_2_CMV-Foward_A04_01.ab1
    1 AGTTCCCCTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA
  181 GGCGGCCATG TCTCTGTGGG GTCTGGTCTC CAAGATGCCC CCAGAAAAAG TGCAGCGGCT
  241 CTATGTCGAC TTTCCCCAAC ACCTGCGGCA TCTTCTGGGT GACTGGCTGG AGAGCCAGCC
  301 CTGGGAGTTC CTGGTCGGCT CCGACGCCTT CTGCTGCAAC TTGGCTAGTG CCCTACTTTC
  361 AGACACTGTC CAGCACCTTC AGGCCTCGGT GGGAGAGCAG GGGGAGGGGA GCACCATCTT
  421 GCAACACATC AGCACCCTTG AGAGCATATA TCAGAGGGAC CCCCTGAAGC TGGTGGCCAC
  481 TTTCAGACAA ATACTTCAAG GAGAGAAAAA AGCTGTTATG GAACAGTTCC GCCACTTGCC
  541 AATGCCTTTC CACTGGAAGC AGGAAGAACT CAAGTTTAAG ACAGGCTTGC GGAGGCTGCA
  601 GCACCGAGTA GGGGAGATCC ACCTTCTCCG AGAAGCCCTG CAGAAGGGGG CTGAGGCTGG
661 CCAAGTGTCT CT
//
Primer:
Sequence file: RDB06383_A3Irb.seq check
>06383_06383_A3Ir_2_Sp6_B04_04.ab1
    1 CCGAGAATCT GCTCGAGCGG CCGATCACCA ACTGGGGTTG GCCCTTAGGT CCATGTGGGA
   61 CATTGAGATC CCAGATTGCC CATAGTGGGA GGGCTGCAGG AGGGGCTGTG CCCCCAAGGA
  121 CCCTCCCCCC GACTCCCCTT GCCCCTCCAG GAGAAGCTTA GTGAGGTCCT GTTCAGTGGG
  181 AGGCAGCAGA GGAGGGAATA TGTCTTCACC AATCCAAGTG CCCTGAGGAA ACGCTGTCAC
  241 TGGCTGGCTC AGGCAGCTGT CTTCCACCAT GGTCACATCT GAGCAGAGCA GGGGGTCAGG
  301 GCTGGACACA GCATGCTCCT GAGGCTGGCA CGGCAGCAGG CCCTGGGGGT GAGGCTGGTC
  361 AAAGGGCAGG CTCATCTGGC CCAGGCTGGG GGGCATCTGC AGGTGAGGCT CCTGGAAGGC
  421 TGACAACACG TTGACTGATT CTTCTGGGGA GAGGCCTTGA TACGGGGGGA TGGAGTGAGA
  481 GTGTGGTGGG TACACCTGGG GCACCATATC TGGGCCAAGC TGCATGCTCA TGGAGGAATC
  541 AGGGGCCATT CCAAGGTCAT AAGAAGGCAC CATGGTAGGC ATCTGGAGCT CTGGGGTAGG
  601 AAGTGGTTGG TCCCTTTCCA CGGTCATCTT GATGGTAGCT GGGACATAAC CCCTGCCATC
661 CTTACCCATC TGTTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles