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pCMFlag_hsPTEN (#RDB06380)

Expression vector of human PTEN.

Alternative name SET-0175_1
Clone info. Expression vector of human PTEN. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano BC005821. PCR cloning, forward primer: atgacagccatcatcaaaga; reverse primer: tcagacttttgtaatttgtgtatgc.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human PTEN1 cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06380.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

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RDB06380 pCMFlag_hsPTEN DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsPTEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06380).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06380_A3Df.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB06380_A3Dfa.seq check
>6380_6380_A3Df_T7long_C08_08.ab1
    1 CAAAGCCTGA ATTCACCATG GACTACAAGG ACGATGACGA TAAGGCGGCC ATGACAGCCA
   61 TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA TTCGACTTAG
  121 ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA AGACTTGAAG
  181 GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG CATAAAAACC
  241 ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA TTTAATTGCA
  301 GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT ATCAAACCCT
  361 TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA GCAATTCACT
  421 GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA CATCGGGGCA
  481 AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC AGAGACAAAA
  541 AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC CTGTTAAAGA
  601 ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT GAAACTATTC
661 CAATGTTCAG TGGC
//
Primer:
Sequence file: RDB06380_A3Dfb.seq check
>6380_6380_A3Df_Sp6long_D08_11.ab1
    1 GCAGAAAACG ATCCTGGATG AATGCTCGAG CGGCCTCAGA CTTTTGTAAT TTGTGTATGC
   61 TGATCTTCAT CAAAAGGTTC ATTCTCTGGA TCAGAGTCAG TGGTGTCAGA ATATCTATAA
  121 TGATCAGGTT CATTGTCACT AACATCTGGT GTTACAGAAG TTGAACTGCT AGCCTCTGGA
  181 TTTGACGGCT CCTCTACTGT TTTTGTGAAG TACAGCTTCA CCTTAAAATT TGGAGAAAAG
  241 TATCGGTTGG CTTTGTCTTT ATTTGCTTTG TCAAGATCAT TTTTTGTTAA AGTAAGTACT
  301 AGATATTCCT TGTCATTATC TGCACGCTCT ATACTGCAAA TGCTATCGAT TTCTTGATCA
  361 CATAGACTTC CATTTTCTAC TTTTTCTGAG GTTTCCTCTG GTCCTGGTAT GAAGAATGTA
  421 TTTACCCAAA AGTGAAACAT TTTGTCCTTT TTTAGCATCT TGTTCTGTTT GTGGAAGAAC
  481 TCTACTTTGA TATCACCACA CACAGGTAAC GGCTGAGGGA ACTCAAAGTA CATGAACTTG
  541 TCTTCCCGTC GTGTGGGTCC TGAATTGGAG GAATATATCT TCACCTTTAG CTGGCAGACC
  601 ACAAACTGAG GATTGCAAGT TCCGCCACTG AACATTGGAA TAGTTTCAAA CATCATCTTG
661 TGAAACAACA GTGCCACT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles