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pCMFlag_hsGATA3 (#RDB06376)

Expression vector of human GATA3.

Alternative name SET-0171_7
Clone info. Expression vector of human GATA3. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano BC006793. PCR cloning, forward primer: gaggtgacggcggaccagcc; reverse primer: ctaacccatggcggtgaccatgc.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human GATA3 cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06376.pdf

Distribution information

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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

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RDB06376 pCMFlag_hsGATA3 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsGATA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06376).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12312_A3K8p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12312_A3K8a_v2.seq check
>06376_12312_A3K8_CMV-Forward_A07_01.ab1
    1 CTTTTCAACT CGGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
  181 AGGCGGCCGA GGTGACGGCG GACCAGCCGC GCTGGGTGAG CCACCACCAC CCCGCCGTGC
  241 TCAACGGGCA GCACCCGGAC ACGCACCACC CGGGCCTCAG CCACTCCTAC ATGGACGCGG
  301 CGCAGTACCC GCTGCCGGAG GAGGTGGATG TGCTTTTTAA CATCGACGGT CAAGGCAACC
  361 ACGTCCCGCC CTACTACGGA AACTCGGTCA GGGCCACGGT GCAGAGGTAC CCTCCGACCC
  421 ACCACGGGAG CCAGGTGTGC CGCCCGCCTC TGCTTCATGG ATCCCTACCC TGGCTGGACG
  481 GCGGCAAAGC CCTGGGCAGC CACCACACCG CCTCCCCCTG GAATCTCAGC CCCTTCTCCA
  541 AGACGTCCAT CCACCACGGC TCCCCGGGGC CCCTCTCCGT CTACCCCCCG GCCTCGTCCT
  601 CCTCCTTGTC GGGGGGCCAC GCCAGCCCGC ACCTCTTCAC CTTCCCGCCC ACCCCGCCGA
  661 AGGACGTCTC CCCGGACCCA TCGCTGTCCA CCCCAGGCTC GGCCGGCTCG GCCCGGCAGG
  721 ACGAGAAAGA GTGCCTCAAG TACCAGGTGC CCCTGCCCGA CAGCATGAAG CTGGAGTCGT
  781 CCCACTCCCG TGGCAGCATG ACCGCCCTGG GTGGAGCCTC CTCGTCGACC CACCACCCCA
  841 TCACCACCTA CCCGCCCTAC GTGCCCGAGT ACAGCTCCGG ACTCTTCCCC CCCCAGCAGC
  901 CTGCTGGGCG GCTCCCCCCA CCGGGCTTCG GATGCAAAGT CCAGGGCCCA AGCCCGGGTC
  961 CCAGCACAGA AAGCAGGGAG TGTGTGAAAC TGTGGGGGCA ACCCTCGACC CCCACTGGTG
 1021 GGCGGCGAGA TGGGCACGGG ACACTAACTG TGCACGCCCT GCCGGCTCTA TCACAAATGG
1081 GACGGACAGA AACCGGGCCC CCCCTCTA
//
Primer: BGH_rev
Sequence file: RDB12312_A3K8b_v2.seq check
>06376_12312_A3K8_BGHrev_B07_04.ab1
    1 GAATAAAAAA TTAGGGTGAC TATAGAATAG GGCCCTCTAG ATGCATGCTC GAGCGGCCTA
   61 ACCCATGGCG GTGACCATGC TGGAGGGGTG GTGTGGTCCA AAGGACAGGC TGGATGGCGG
  121 GTGCATCGGC GTGGGCGTGG TCAGCATGTG GCTGGAGTGG CTGAAGGGCG AGATGTGGCT
  181 CAGGGAGGAC ATGTGTCTGG AGAGGGCGGC CGGGTTAAAC GAGCTGTTCT TGGGGAAGTC
  241 CTCCAGTGAG TCATGCACTT TTTTGCACTT TTTGGATTTG CTAGACATTT TTCGGTTTCT
  301 GGTCTGGATG CCTTCCTTCT TCATAGTCAG GGGTCTGTTA ATATTGTGAA GCTTGTAGTA
  361 GAGCCCACAG GCATTGCAGA CAGGGTCCCC ATTGGCATTC CTCCTCCAGA GTGTGGTTGT
  421 GGTGGTCTGA CAGTTCGCAC AGGACGTCCC TGCTCTCCTG GCTGCAGACA GCCTTCGCTT
  481 GGGCTTAATG AGGGGCCGGT TCTGTCCGTT CATTTTGTGA TAGAGCCCGC AGGCGTTGCA
  541 CAGGTAGTGT CCCGTGCCAT CTCGCCGCCA CAGTGGGGTC GAGGTTGCCC CACAGTTCAC
  601 ACACTCCCTG CCTTCTGTGC TGGACCGGGC CTTGGGCCTG GACTTGCATC CGAAGCCGGT
  661 GGGGGAGCCG CCCAGCAGGC TGCTGGGGGG GAAGAGTCCG GAGCTGTACT CGGGCACGTA
  721 GGGCGGGTAG GTGGTGATGG GGTGGTGGGT CGACGAGGAG GCTCCACCCA GGGCGGTCAT
  781 GCTGCCACGG GAGTGGGACG ACTCCAGCTT CATGCTGTCG GGCAGGGGCA CCTGGTACTT
  841 GAGGCACTCT TTCTCGTCCT GCCGGGCCGA GCCGGCCGAG CCTGGGGGTG GACAGCGATG
  901 GGTCCGGGGG AGACGTCCTT CGGCGGGGTG GGCGGGAAGG TGAAGAGGTG CGGGCTGGCG
  961 TGGCCCCCCC GACAAGGAGA GGACGAGCCC GGGGGGTAGA CGGAGAGGGG CCCGGGAGCG
 1021 TGTTGTATGG ACGTCTTGAG AGGCTGAAAT CATGGGAGGC GGTGTTGTTG GCCTGGCTCT
1081 CAAGGGGCCT
//
Primer: SV40pro_F_V2
Sequence file: RDB12312_A3K8c_v2.seq check
>06376_12312_A3K8_SV40pro_F_V2_C07_07.ab1
    1 GGGAACCGTT TTTTGGGAGG CCTAGGCTTT TGCAAAAGCT CCCGGGAGCT TGGATATCCA
   61 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
  121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
  181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
  241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
  301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
  361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
  421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
  481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
  541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
  601 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
  661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
  721 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
  781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
  841 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG
  901 GACTCTGGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTTC
  961 GATTCCACCG CCGCCCTTCT ATGAAAGGGT TGGGGCTTCG GGATCGTTTT TCCCGGGACG
 1021 CCCGGCTGGG ATGGATCCTT CCCAGCGCGG GGATCTCATG GCTGGGAGTT CTTCGACCAC
1081 CCCAACTTGG ATTATTGCCA GCTTATAATG TACGAATTAA GCCGAGTTAA C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles