RDB06375 - pCMFlag

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCMFlag_hsFLI1 (#RDB06375)

Expression vector of human FLI1.

Alternative name	SET-0170_21
Clone info.	Expression vector of human FLI1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment	Derived from Sugano BC001670. PCR cloning, forward primer: gacgggactattaaggaggc; reverse primer: ctagtagtagctgcctaagtgtgaag.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human FLI-1 cDNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB06375.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06375	pCMFlag_hsFLI1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsFLI1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06375).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15915_A8Dgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB15915_A8Dga.seq
>06375_15915_A8Dg_1_CMV-Forward_A01_01_ABI08.ab1 1 TTTTTAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC 121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA 181 GGCGGCCGAC GGGACTATTA AGGAGGCTCT GTCGGTGGTG AGCGACGACC AGTCCCTCTT 241 TGACTCAGCG TACGGAGCGG CAGCCCATCT CCCCAAGGCC GACATGACTG CCTCGGGGAG 301 TCCTGACTAC GGGCAGCCCC ACAAGATCAA CCCCCTCCCA CCACAGCAGG AGTGGATCAA 361 TCAGCCAGTG AGGGTCAACG TCAAGCGGGA GTATGACCAC ATGAATGGAT CCAGGGAGTC 421 TCCGGTGGAC TGCAGCGTTA GCAAATGCAG CAAGCTGGTG GGCGGAGGCG AGTCCAACCC 481 CATGAACTAC AACAGCTATA TGGACGAGAA GAATGGCCCC CCTCCTCCCA ACATGACCAC 541 CAACGAGAGG AGAGTCATCG TCCCCGCAGA CCCCACACTG TGGACACAGG AGCATGTGAG 601 GCAATGGCTG GAGTGGGCCA TAAAGGAGTA CAGCTTGATG GAGATCGACA CATCCTTTTT 661 CCAGAACATG GATGGCAAGG AACTGTGTAA AATGAACAAG GAGGACTTCC TCCGCGCCAC 721 CACCCTCTAC AACACGGAAG TGCTGTTGTC ACACCTCAGT TACCTCAGGG AAAGTTCACT 781 GCTGGCCTAT AATACAACCT CCCACACCGA CCAATCCTCA CGATTGAGTG TCAAAGAAGA 841 CCCTTCTTAT GACTCAGTCA GAAGAGGAGC TTGGGGCAAT AACATGAATT CTGGCCTCAA 901 CAAAAGTCCT CCCCTTGGAG GGGCACAAAC GATCAGTAAG AATACAGAGC AACGGCCCCA 961 GCCAGATCCG TATCAGATCC TGGGCCCGAC CAGCAGTCGC CCTAGCCCAA CCCTGGAAGC 1021 GGGCAGATCC CAGCTGTGGC AATTTCCTCC TGAGCTGCTC TCCGACAGCG CCACGCAGGC 1081 TGTATCACCT GCGAGGGCAC GACGAGAAGT CCAAATTGAC GGAACCCCGA TGAGGTGACC 1141 AAGGCCGCCC G //
Primer: hsFLI1_F2 Sequence file: RDB15915_A8Dgb.seq
>06375_15915_A8Dg_1_hsFLI1_F2_A02_02_ABI24.ab1 1 ACGGGGGCCC CAAGCCAGAT CCGTATCAGA TCCTGGGCCC GACCAGCAGT CGCCTAGCCA 61 ACCCTGGAAG CGGGCAGATC CAGCTGTGGC AATTCCTCCT GGAGCTGCTC TCCGACAGCG 121 CCAACGCCAG CTGTATCACC TGGGAGGGGA CCAACGGGGA GTTCAAAATG ACGGACCCCG 181 ATGAGGTGGC CAGGCGCTGG GGCGAGCGGA AAAGCAAGCC CAACATGAAT TACGACAAGC 241 TGAGCCGGGC CCTCCGTTAT TACTATGATA AAAACATTAT GACCAAAGTG CACGGCAAAA 301 GATATGCTTA CAAATTTGAC TTCCACGGCA TTGCCCAGGC TCTGCAGCCA CATCCGACCG 361 AGTCGTCCAT GTACAAGTAC CCTTCTGACA TCTCCTACAT GCCTTCCTAC CATGCCCACC 421 AGCAGAAGGT GAACTTTGTC CCTCCCCATC CATCCTCCAT GCCTGTCACT TCCTCCAGCT 481 TCTTTGGAGC CGCATCACAA TACTGGACCT CCCCCACGGG GGGAATCTAC CCCAACCCCA 541 ACGTCCCCCG CCATCCTAAC ACCCACGTGC CTTCACACTT AGGCAGCTAC TACTAGGGCC 601 GCTCGAGCAT GCATCTAGAG GGCCCTATTC TATAGTGTCA CCTAAATGCT AGAGCTCGCT 661 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC 721 CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG 781 CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGGTGGG GCAGGACAGC 841 AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT 901 TCTGAGGCGG AAAGAACCAG CTGGGGCTCG AGGGGGGATC CCCACGCGCC CTGTAGCGGC 961 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 1021 CTAGCGCCCG CTCTTTCGCT TTTCTTCCCT TCCTTTCTCG CCACGTCGCC GGCTTCCCCG 1081 TCAGCTCTAA TCGGGCTCCT TAAGGATCCG ATTATGCTTT ACGGCACCTC GACCTCCTAA 1141 AAACTGAATA AGGGTGGTAT ATGAT //
Primer: SV40pro_F Sequence file: RDB15915_A8Dgc.seq
>06375_15915_A8Dg_1_SV40pro_F_B01_02_ABI08.ab1 1 TGGGGGCACT TCGGGACCGG CTTGGGGTAG CATTCAGAGT AGTGAGGGAG GCTTTTTTGG 61 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG GATCTGATCA 121 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC 181 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC 241 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA 301 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC 361 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT 421 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA 481 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC 541 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT 601 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC 661 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG 721 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT 781 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT 841 TGGCGGCGAA TGGGGCTGAC CGCTTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 901 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGGAC TCTGGGGTTC 961 GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC 1021 TTCTATGAAG GTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTTGGATG AATCCTCCAG 1081 CGCGGGGGAA TCTCATGCTG GAGGTTCTTC GCCCCACCCT CAACTGTTAT GGCCAGGCTG 1141 ATATGGTTAC AAGTAAGGCA AATAGGCCAT TCACCAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

human FLI-1

External Database
Human FLI-1

Reference sequence