Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
[open/close]
Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsRORA (#RDB06373)
Expression vector of human RORA.
| Alternative name | SET-0091_2 |
|---|---|
| Clone info. | Expression vector of human RORA. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | Derived from Sugano BC008831. PCR cloning, forward primer: GAGTCAGCTCCGGCAGCCCC; reverse primer: TTACCCATCAATTTGCATTGCTGGCTC. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human ROR alpha cDNA |
| Depositor|Developer | DNA Bank, | |
| Remarks, protocol and/or map (pdf) | RDB06373.pdf |
|---|
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06373 | pCMFlag_hsRORA | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsRORA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06373). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB06373_A3Ir.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
|
Primer: Sequence file: RDB06373_A3Ira.seq
|
|---|
|
>06373_06373_A3Ir_1_CMV-Foward_G11_20.ab1 1 AGTAATCACC TCCCGCCCCC CTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT 121 AATACGACTC ACTATAGGGA GACCCAAGCT GAATTCACCA TGGACTACAA GGACGATGAC 181 GATAAGGCGG CCGAGTCAGC TCCGGCAGCC CCCGACCCCG CCGCCAGCGA GCCAGGCAGC 241 AGCGGCGCGG ACGCGGCCGC CGGCTCCAGG GAGACCCCGC TGAACCAGGA ATCCGCCCGC 301 AAGAGCGAGC CGCCTGCCCC GGTGCGCAGA CAGAGCTATT CCAGCACCAG CAGAGGTATC 361 TCAGTAACGA AGAAGACACA TACATCTCAA ATTGAAATTA TTCCATGCAA GATCTGTGGA 421 GACAAATCAT CAGGAATCCA TTATGGTGTC ATTACATGTG AAGGCTGCAA GGGCTTTTTC 481 AGGAGAAGTC AGCAAAGCAA TGCCACCTAC TCCTGTCCTC GTCAGAAGAA CTGTTTGATT 541 GATCGAACCA GTAGAAACCG CTGCCAACAC TGTCGATTAC AGAAATGCCT TGCCGTAGGG 601 ATGTCTCGAG ATGCTGTAAA ATTTGGCCGA ATGTCAAAAA AGCAGAGAGA CAGCTTGTAT 661 GCAGAAGTAC AGAAA // |
|
Primer: Sequence file: RDB06373_A3Irb.seq
|
|
>06373_06373_A3Ir_1_Sp6_H03_24.ab1 1 CCTAATAGCA GCTCGAGCGG CTTACCCATC AATTTGCATT GCTGGCTCAA ATTCTGAAGT 61 GAACAACTCC TTGTATAATG GAGGAAAATG AAGTCGCACA ATGTCTGGGT ATATTGCTTT 121 AAATGCCATT AGCTTTTCTG TATGTCGTCC ACATAAGGCT CTTAATGTAG ACACCTTGCA 181 TATTAACTTT GTTAGTATTC CATCTTCTCG GTGATTCTTC TGTAGGACGT GTTGAAGAGC 241 TAGCTGAATT TTCTGTTGCA GTTTTTCAAT TTTTACCTTT TCTTGCAGCC ATGAGCGATC 301 TGCTGACATC AGTACAAATG CAGAAAATAA TGCAATTTCA TCTTCAGTCA GGTGCATAGA 361 ACATAAACTC TTTCCAAATT CAAACACAAA GCTAATAAAG TCTTCACAAC CTAAGGATTT 421 GAAGACGTCG GGGCTGGCAT ACTTCCCATC AAAGTACACG GTGTTGTTCT GAGAGTCAAA 481 GGCACGGCAC ATTCTGATAA ACACCACCTC TAGAGAACCT GCTTTTAGAA GCACAATTTG 541 ATCATTTTGA CACAGTTCCA TAAATCCATC AATGCGTTTG GCAAACTCCA CCACATACTG 601 TATAGCTTCT GTAATTTTGA TGGCACACAA TTGCCACATC ACCTCCCGCT GCTTGTTTTG 661 ATAGTTCTCA ATT // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human ROR alpha
Reference sequence
Tips
Featured content