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pCMFlag_hsNR4A2

Expression vector of human NURR1.

Catalog number RDB06298
Resource name pCMFlag_hsNR4A2
Alternative name SET-0078_2
Clone info. Expression vector of human NURR1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano BC009288. PCR cloning, forward primer: ATGCCTTGTGTTCAGGCGCA; reverse primer: GCTTGGGAGGAGGTCTTAGAAAGG.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human NURR1 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human NURR1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06298 pCMFlag_hsNR4A2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB06298_A3Ir.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB06298_A3Ira.seq check
          >06298_06298_A3Ir_CMV-Foward_E04_13.ab1
              1 AGTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
            181 CGGCCATGCC TTGTGTTCAG GCGCAGTATG GGTCCTCGCC TCAAGGAGCC AGCCCCGCTT
            241 CTCAGAGCTA CAGTTACCAC TCTTCGGGAG AATACAGCTC CGATTTCTTA ACTCCAGAGT
            301 TTGTCAAGTT TAGCATGGAC CTCACCAACA CTGAAATCAC TGCCACCACT TCTCTCCCCA
            361 GCTTCAGTAC CTTTATGGAC AACTACAGCA CAGGCTACGA CGTCAAGCCA CCTTGCTTGT
            421 ACCAAATGCC CCTGTCCGGA CAGCAGTCCT CCATTAAGGT AGAAGACATT CAGATGCACA
            481 ACTACCAGCA ACACAGCCAC CTGCCCCCCC AGTCTGAGGA GATGATGCCG CACTCCGGGT
            541 CGGTTTACTA CAAGCCCTCC TCGCCCCCGA CGCCCACCAC CCCGGGCTTC CAGGTGCAGC
            601 ACAGCCCCAT GTGGGACGAC CCGGGATCTC TCCACAACTT CCACCAGAAC TACGTGGCCA
          661 CTACGCACAT G
          //
          Primer:
          Sequence file: RDB06298_A3Irb.seq check
          >06298_06298_A3Ir_Sp6_F04_16.ab1
              1 GCTGAATCCT GCTCGAGCGG CCGCTTGGGA GGAGGTCTTA GAAAGGTAAA GTGTCCAGGA
             61 AAAGTTTGTC AATTATTGCT GGCGGTGGCA CCAAGTCTTC CAATTTCAGG TAGAAAATGC
            121 GCTGTAGCCC CTGTGTGCAA AGGGTACGAA GTTCTGGGAG CTTCCCCAAC AGTTTGGACA
            181 AATAATTGGG GCGGTTCAAC CCCCCATTGT TGAAAGTCAC GTGGTCTTTG AGACAATTTA
            241 CAATCTTGTT TTGCAGTTCT TCCACTCTCT TGGGTTCCTT GAGCCCGTGT CTCTCTGTGA
            301 CCATAGCCAG GGCAGCAATG CAGGAGAAGG CAGAAATGTC GATGTTCATA TTCTGCAAGT
            361 TGGAGGAGAA TTCAACAATG GAATCAATCC ATTCCCCAAA GCCACGAACG CATTGCAACC
            421 TGTGCAAGAC CACCCCATTG CAAAAGATGA GTTTACCCTC CACTGGGTTG GACCTGTATG
            481 CTAATCGAAG GACAAACAGT TCTAAGAAAG CTGATTCAAA AAGCAGGTCT TGGTCGGCTT
            541 TGGGCAGGTC TGCGAAGCCA GGGATCTTCT CTGCCCAGCC CCGGATGATC TCCATGGAGC
            601 CAGTCAGGAG ATCATAGAAT TGCTGGATAT GCTGGGTGTC ATCTCCACTC ATTTGATAGT
          661 CAGGGTTCGC CTGGAAC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl