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pCMFlag_hsPPARA

Expression vector of human PPARA.

Catalog number RDB06268
Resource name pCMFlag_hsPPARA
Alternative name SET-0081_20
Clone info. Expression vector of human PPARA. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gtggacacggaaagcccact; reverse primer: gctgatctgaaggaactcagt. Nucleotide substitution A1442T to NM_005036.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human PPARA/PPAR alpha cDNA
Depositor|Developer DNA Bank, |
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External Database
Human PPARA/PPAR alpha

          Reference sequence
            

          Distribution information

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          RDB06268 pCMFlag_hsPPARA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06268.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15913_A8D1p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB15913_A8D1a.seq check
          >06268_15913_A8D1_1_CMV-Forward_A01_01_ABI08.ab1
              1 ATAATCAACT TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
            121 TACGACTCAC TATAGGGAGA CCCAAGCTGA ATTCACCATG GACTACAAGG ACGATGACGA
            181 TAAGGCGGCC GTGGACACGG AAAGCCCACT CTGCCCCCTC TCCCCACTCG AGGCCGGCGA
            241 TCTAGAGAGC CCGTTATCTG AAGAGTTCCT GCAAGAAATG GGAAACATCC AAGAGATTTC
            301 GCAATCCATC GGCGAGGATA GTTCTGGAAG CTTTGGCTTT ACGGAATACC AGTATTTAGG
            361 AAGCTGTCCT GGCTCAGATG GCTCGGTCAT CACGGACACG CTTTCACCAG CTTCGAGCCC
            421 CTCCTCGGTG ACTTATCCTG TGGTCCCCGG CAGCGTGGAC GAGTCTCCCA GTGGAGCATT
            481 GAACATCGAA TGTAGAATCT GCGGGGACAA GGCCTCAGGC TATCATTACG GAGTCCACGC
            541 GTGTGAAGGC TGCAAGGGCT TCTTTCGGCG AACGATTCGA CTCAAGCTGG TGTATGACAA
            601 GTGCGACCGC AGCTGCAAGA TCCAGAAAAA GAACAGAAAC AAATGCCAGT ATTGTCGATT
            661 TCACAAGTGC CTTTCTGTCG GGATGTCACA CAACGCGATT CGTTTTGGAC GAATGCCAAG
            721 ATCTGAGAAA GCAAAACTGA AAGCAGAAAT TCTTACCTGT GAACATGACA TAGAAGATTC
            781 TGAAACTGCA GATCTCAAAT CTCTGGCCAA GAGAATCTAC GAGGCCTACT TGAAGAACTT
            841 CAACATGAAC AAGGTCAAAG CCCGGGTCAT CCTCTCAGGA AAGGCCAGTA ACAATCCACC
            901 TTTTTGTCAT ACATGATATG GAGACACTGG TGTATGGCTG GAGAAAGACG CTGGTGGCCA
            961 AGCCTGGTGG GCCAATGGCA TCCAAGAAAC AAGGAAGCGG AGGTCCCGCA TCTTCACTGC
           1021 TGCCAGTGCA CGTCAGTGGA GACCGTCACG GAAGCTCCAC GATTCGCCAA GTCATCCAAG
          1081 GCTTCGCCAA CTTGGACCTG AACCGAATC
          //
          Primer: bGH_rev2
          Region: bGHpA,insert 3'
          Sequence file: RDB15913_A8D1b.seq check
          >06268_15913_A8D1_1_BGH_rev2_B01_02_ABI08.ab1
              1 GAGTAGGGTT AAGGAAGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA AACTAGAAGG
             61 CACAGTCGAG GCTGATCAGC GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC
            121 TAGATGCATG CTCGAGCGGC CGCTGATCTG AAGGAACTCA GTACATGTCC CTGTAGATCT
            181 CCTGCAGTAG CGGGTGCAGC GCAGCATCCG ACTCCGTCTT CTTGATGATC TGCACCAGCT
            241 GCGCATGCTC CGTCACCAGC TGCCGGAGGT CTGCCATTTT TTGAAGAAGT TTTGGGAAGA
            301 GAAAGATATC GTCCGGGTGG TTGCTCTGCA GGTGGAGTCT GAGCACATGT ACAATACCCT
            361 CCTGCATTTT TTCAATGTGT CCTACGTTTA GAAGGCCAGG ACGATCTCCA CAGCAAATGA
            421 TAGCAGCCAC AAAAAGGGAG ATATCACTGT CATCCAGTTC CAGTGCATTG AACTTCATGG
            481 CAAAATCAAA CTTGGGTTCC ATGATATCAC AGAACGGTTT CCTTAGGCTT TTTAGGAATT
            541 CACGAGTTAT AAACCCATTT CCATACGCTA CCAGCATCCC GTCTTTGTTC ATCACAGAAG
            601 ACAGCATGGC GAATATGGCC TCATAAACTC CGTATTTTAG CAATGTCACT TGATCGTTCA
            661 GGTCCAAGTT TGCGAAGCCT GGGATGGCCT TGGCGAATTC CGTGAGCTCC GTGACGGTCT
            721 CCACTGACGT GCACTGGCAG CAGTGAAAGA TGCGGACCTC CGCCTCCTTG TTCTGGATGC
            781 CATTGGCCAC CAGCTTGGCC ACCAGCGTCT TCTCAGCCAT ACACAGTGTC TCCATATCAT
            841 GTATGACAAA AGGTGGATTG TTACTGGCCT TTCCTGAGAG GATGACCCGG GGCTTTGACC
            901 TTGTTCATGT TGAAGTTTCT TCAAGTAAGG CCTCGTAGAT TCTCTTGGCC AGAGATTTGG
            961 AGATCTGCAG TTTCAGAAAT CTTCTATGTC ATGTTCACAG GGTAAGAATT TCTGCTTTTC
           1021 CAGTTTTGGC TTTTCTCAGA TCTTTGGCCA ATCCGGTTCC CAAAACGGAA TCGCGTGTGT
          1081 GACATTCTGA ACGAAGGCAA CTTGGTGGAA AT
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB15913_A8D1c.seq check
          >06268_15913_A8D1_SV40pro_F_A01_01_ABI08.ab1
              1 CCGGCCCGTT AACATTCGGA AGTAGTGGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAGCTCCCG GGAGCTTGGA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
            121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
            661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGGCTG
            841 ACCGCTTCCT CGTGCTTTAC GGGTATCGCC GCTCCCGGAT TCGCAGCGCA TCGGCCTTTC
            901 TATCGGCCTT TCTTGACGAG TTTCTTTCTT GAGCGGGACT TCTGGGTTTC GAAAATTGAC
            961 CGACCAAGCG ACGCCCAACT GCCATCACGA GATTTCGAAT TTCCACCGCC CGCCTTTCTT
           1021 ATGAAAAGGT TGGGGCTTCG GAATTCGGTT TTTCCGGAAC GGCCCGGCCT GAATTGGATC
          1081 CTCAAGCGCG GAATCTCATG CCTGGGAGAT TCCTTCCGTC CGAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl