Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsPPARA (#RDB06268)
Expression vector of human PPARA.
| Alternative name | SET-0081_20 |
|---|---|
| Clone info. | Expression vector of human PPARA. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | PCR cloning, forward primer: gtggacacggaaagcccact; reverse primer: gctgatctgaaggaactcagt. Nucleotide substitution A1442T to NM_005036. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Gene/insert name | Human PPARA/PPAR alpha cDNA |
| Depositor|Developer | DNA Bank, | |
| Remarks, protocol and/or map (pdf) | RDB06268.pdf |
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Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06268 | pCMFlag_hsPPARA | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsPPARA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06268). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB15913_A8D1p1-3.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV_Forward (Pr0016) Region: CMV pro,FLAG,insert 5' Sequence file: RDB15913_A8D1d.seq
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>06268_15913_A8D1_1_CMV-Forward_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGCNNAN GGGCGGTAGG CGTGTACGGT GGGANGTCTA 61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA 121 TACGACTCAC TATAGGGAGA CCCAAGCTGA ATTCACCATG GACTACAAGG ACGATGACGA 181 TAAGGCGGCC GTGGACACGG AAAGCCCACT CTGCCCCCTC TCCCCACTCG AGGCCGGCGA 241 TCTAGAGAGC CCGTTATCTG AAGAGTTCCT GCAAGAAATG GGAAACATCC AAGAGATTTC 301 GCAATCCATC GGCGAGGATA GTTCTGGAAG CTTTGGCTTT ACGGAATACC AGTATTTAGG 361 AAGCTGTCCT GGCTCAGATG GCTCGGTCAT CACGGACACG CTTTCACCAG CTTCGAGCCC 421 CTCCTCGGTG ACTTATCCTG TGGTCCCCGG CAGCGTGGAC GAGTCTCCCA GTGGAGCATT 481 GAACATCGAA TGTAGAATCT GCGGGGACAA GGCCTCAGGC TATCATTACG GAGTCCACGC 541 GTGTGAAGGC TGCAAGGGCT TCTTTCGGCG AACGATTCGA CTCAAGCTGG TGTATGACAA 601 GTGCGACCGC AGCTGCAAGA TCCAGAAAAA GAACAGAAAC AAATGCCAGT ATTGTCGATT 661 TCACAAGTGC CTTTCTGTCG GGATGTCACA CAACGCGATT CGTTTTGGAC GANTGCCAAG 721 ATCTGAGAAA GCAAAACTGA AAGCAGAAAT TCTTACCTGT GAACATGACA TAGAANNNTC 781 TGAAACTGCA GATCTCAAAT CTCTGNCCAA NAGAATCTAC NNNGNCTACT TGAAGANNTT 841 CAACATGAAC AAGGTCAAAG CNNGGNNCAT CCTCTCANGA AAGGNCAGTA ACAATCCANC 901 NTTTNGNNNT ACNTGATATN GAGACNCNNG TGTATNNNNN NANNANNANN NTGGNNGNCA 961 AGNNTNGNNG NNNNANNNNN NNNNN // |
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Primer: bGH_rev2 (Pr0606) Region: bGH pA,insert 3' Sequence file: RDB15913_A8D1e.seq
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>06268_15913_A8D1_1_CMV-Forward_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGCNNAN GGGCGGTAGG CGTGTACGGT GGGANGTCTA 61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA 121 TACGACTCAC TATAGGGAGA CCCAAGCTGA ATTCACCATG GACTACAAGG ACGATGACGA 181 TAAGGCGGCC GTGGACACGG AAAGCCCACT CTGCCCCCTC TCCCCACTCG AGGCCGGCGA 241 TCTAGAGAGC CCGTTATCTG AAGAGTTCCT GCAAGAAATG GGAAACATCC AAGAGATTTC 301 GCAATCCATC GGCGAGGATA GTTCTGGAAG CTTTGGCTTT ACGGAATACC AGTATTTAGG 361 AAGCTGTCCT GGCTCAGATG GCTCGGTCAT CACGGACACG CTTTCACCAG CTTCGAGCCC 421 CTCCTCGGTG ACTTATCCTG TGGTCCCCGG CAGCGTGGAC GAGTCTCCCA GTGGAGCATT 481 GAACATCGAA TGTAGAATCT GCGGGGACAA GGCCTCAGGC TATCATTACG GAGTCCACGC 541 GTGTGAAGGC TGCAAGGGCT TCTTTCGGCG AACGATTCGA CTCAAGCTGG TGTATGACAA 601 GTGCGACCGC AGCTGCAAGA TCCAGAAAAA GAACAGAAAC AAATGCCAGT ATTGTCGATT 661 TCACAAGTGC CTTTCTGTCG GGATGTCACA CAACGCGATT CGTTTTGGAC GANTGCCAAG 721 ATCTGAGAAA GCAAAACTGA AAGCAGAAAT TCTTACCTGT GAACATGACA TAGAANNNTC 781 TGAAACTGCA GATCTCAAAT CTCTGNCCAA NAGAATCTAC NNNGNCTACT TGAAGANNTT 841 CAACATGAAC AAGGTCAAAG CNNGGNNCAT CCTCTCANGA AAGGNCAGTA ACAATCCANC 901 NTTTNGNNNT ACNTGATATN GAGACNCNNG TGTATNNNNN NANNANNANN NTGGNNGNCA 961 AGNNTNGNNG NNNNANNNNN NNNNN // |
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Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,NeoR Sequence file: RDB15913_A8D1f.seq
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>06268_15913_A8D1_1_SV40pro_F_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNAANNNNN NGNNNNNNNT TTTNNNNNNN NGCCTAGGCT 61 TTTGCNNNNN ANCTCCCGGG AGCTTGNNTN TCCATTTTCG GATCTGATCA AGAGACAGGA 121 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 181 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 241 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 301 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 361 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 421 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 481 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 541 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 601 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CNNNNNCAAG 661 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 721 ATCATGGTGG AAAATGGCCG CTTTTCTGGA NTCATCGACT GTGGCCNGCT GGGTGTGGCG 781 GACCGCTATC AGGANATAGC GTTGGCTACC CGTGATATTG CTGAANAGCT TGGCGGCNAN 841 NGGGCTGANC GCTTCCTCGT GCTTTACNGN ATCNCCGNTC CCGATNNNCA GCGCATCGCC 901 NTCNATCGCC NTNCNTGACN NNNNNTNNNN ANCGGGANTC NNGGGGTTNN GAAANTGACC 961 GANNNNANCN NNNNNNNNN // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human PPARA/PPAR alpha
Reference sequence
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