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pCMFlag_hsNFE2L2 (#RDB06265)

Expression vector of human NFE2L2.

Alternative name SET-0075_47
Clone info. Expression vector of human NFE2L2. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: atgatggacttggagctgccg; reverse primer: ctagtttttcttaacatctggctt.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human NRF2; NFE2L2 cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06265.pdf

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RDB06265 pCMFlag_hsNFE2L2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsNFE2L2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06265).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06265_A7Gop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB06265_A7Goa.seq check
>6265_6265_A7Go_CMV-Forward_A01_01_ABI08.ab1
    1 CCTCAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
  181 CGGCCATGAT GGACTTGGAG CTGCCGCCGC CGGGACTCCC GTCCCAGCAG GACATGGATT
  241 TGATTGACAT ACTTTGGAGG CAAGATATAG ATCTTGGAGT AAGTCGAGAA GTATTTGACT
  301 TCAGTCAGCG ACGGAAAGAG TATGAGCTGG AAAAACAGAA AAAACTTGAA AAGGAAAGAC
  361 AAGAACAACT CCAAAAGGAG CAAGAGAAAG CCTTTTTCGC TCAGTTACAA CTAGATGAAG
  421 AGACAGGTGA ATTTCTCCCA ATTCAGCCAG CCCAGCACAT CCAGTCAGAA ACCAGTGGAT
  481 CTGCCAACTA CTCCCAGGTT GCCCACATTC CCAAATCAGA TGCTTTGTAC TTTGATGACT
  541 GCATGCAGCT TTTGGCGCAG ACATTCCCGT TTGTAGATGA CAATGAGGTT TCTTCGGCTA
  601 CGTTTCAGTC ACTTGTTCCT GATATTCCCG GTCACATCGA GAGCCCAGTC TTCATTGCTA
  661 CTAATCAGGC TCAGTCACCT GAAACTTCTG TTGCTCAGGT AGCCCCTGTT GATTTAGACG
  721 GTATGCAACA GGACATTGAG CAAGTTTGGG AGGAGCTATT ATCCATTCCT GAGTTACAGT
  781 GTCTTAATAT TGAAAATGAC AAGCTGGTTG AGACTACCAT GGTTCCAAGT CCAGAAGCCA
  841 AACTGACAGA AGTTGACAAT TATCATTTTT TACTCATCTA TACCCTCAAT GGGAAAAAAG
  901 AAGTAGGTAA CTGTAGTCCA CATTTTTCTT TAATGCTTTT TGAGGGATTC CCTTTCAGCA
  961 GCATCCCTCT CCACAGAAGA CCCCAACCCA GTTGACAGTG AACTCATTAA AATTCAGATG
 1021 CCACAGTCCA ACCACAGATT TTTGGTGAAT GAAGTTTTAA TTCCTGCTTT TCATAGCTTG
1081 AAGCCAAGTA TTCAGCAACA GCATTGACCC CGTTCAGCG
//
Primer: BGH_rev2
Sequence file: RDB06265_A7Gob.seq check
>06265_06265_A7Go_BGH_rev2_A01_01_ABI24.ab1
    1 CTTACGGGTT CAGTAGGCAC GGGGGAGGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGA GCTCTAGCAT TTAGGTGACA CTATAGAATA GGGCCCTCTA
  121 GATGCATGCT CGAGCGGCCC TAGTTTTTCT TAACATCTGG CTTCTTACTT TTGGGAACAA
  181 GGAAAACATT GCCATCTCTT GTTTGCTGCA GGGAGTATTC ACTAGGAGAA TAAGGTTTTC
  241 CATCTTCATC ACGTAGCATG CTGAAAACTT CGAGATATAA GGTGCTGAGT TGTTTTTTCA
  301 GTAGGTGAAG GCTTTTGTCA TTTTCTCCTT TTTCTTTGAG CAATTTTTCT TTTTCATCTT
  361 TCAAATGATC TAAATCTTGC TCTAGTTCTA CTATATTTTC CAGTTTTCTT TTTCTGCAAT
  421 TCTGAGCAGC CACTTTATTC TTACCCCTCC TACGTATATC CCGAATTAAT GCAAGTTGAG
  481 CTTCATTGAA CTGCTCTTTG GACATCATTT CGTTGAAGTC AACAACAGGG AGGTTAATGA
  541 TTTTTTCTAC AGGGAATGGG ATATGGAGAG CTTTTGCCCT AAGTTCATCT CTTGTGAGAT
  601 GAGCCTCCAA GCGGCTTGAA TGTTTGTCTT TTGTGAATGG GGTTTTCCGA TGACCAGGAC
  661 TTACAGGCAA TTCTTTCTCT GGTGTGTTCT CACATTGGGC ATCATGCACG TGAGTGCTCT
  721 GCCCCTGAGA TGGTGACAAG GGTTGTACCA TATCCCCAGA AGAATGTACT GGTGTTTTAG
  781 GACCATTCTG TTTGACACTT CCAGGGGCAC TATCTAGCTC TTCCACTTCA GAATCACTGA
  841 GGCCAAGTAG TGTGTCTCCA TAGCTGGAAG ATTCCACTGA GTGTTCTGGT GATGCCACAC
  901 TGGGACTTGT GTTTAGTGAA ATGCCGGAGT CAGAATCATT GAATTCTGCT GTGCTTTCAG
  961 GGTGGTTTTG GTTGAAAGCT TTGCAAAGTG ATAGATCAGA AAACATCAAA TGGGCCCATT
 1021 TAAGAAAGTT TCAGAGGAGT GAATGGCTTA AAAGTAGCAC TGAGGGCCAT GCTTGTTGCT
 1081 GATACTTAGC TCAGCTATGA AGCCAGAATA AAAATTCCAT CACAAAAAAT TCTTGTGTTT
1141 GAACCTTGTT TG
//
Primer: SV40pro_F_V2
Sequence file: RDB06265_A7Goc.seq check
>6265_6265_A7Go_SV40pro_F_V2_C01_03_ABI08.ab1
    1 GTCCAGGCTT TTTTTGGGGA GGGCTAGGCT TTTGCAAAAG CTCCCGGGGG AGCTTGGATA
   61 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT
  121 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA
  181 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG
  241 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG
  301 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT
  361 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT
  421 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG
  481 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT
  541 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC
  601 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GGCGCGCATG CCCGACGGCG AGGATCTCGT
  661 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGGT GGGAAAATGG CCGCTTTTCT
  721 GGATTTCATC GACTGTGGCC GGCTGGGTGT GGGCGGACCG CTATCAGGAC ATAGCGTTGG
  781 CTACCCCGTG ATATTTGCTG AAGAGCTTGG CGGCGAAATG GGGCTGACCG CTTTCCTCGT
  841 GCCTTTTACG GGAATCGCCC GCTCCCGATT TCGCAGCGCG ATCGGCCTTT CTTAATCGCC
  901 TTCTTTGGAC GGAGTTTCTT TCTGGAGCGG GGAATTCCTG AGGGTTTCGA AAATGGAACC
961 GAACCAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles