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pCMFlag_hsESRRA

Expression vector of human ESRRA.

Catalog number RDB06260
Resource name pCMFlag_hsESRRA
Alternative name SET-0032_4
Clone info. Expression vector of human ESRRA. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: atgtccagccaggtggtgggc; reverse primer: tcagtccatcatggcctcgagc.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human ERR alpha cDNA
Depositor|Developer DNA Bank, |
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External Database
Human ERR alpha

          Reference sequence
            

          Distribution information

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          RDB06260 pCMFlag_hsESRRA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06260.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12739_A7Jbp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12739_A7Jba.seq check
          >06260_12739_A7Jb_CMV-Forward_A01_01_ABI08.ab1
              1 AGTGTTCAAC TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTGAA TTCACCATGG ACTACAAGGA CGATGACGAT
            181 AAGGCGGCCA TGTCCAGCCA GGTGGTGGGC ATTGAGCCTC TCTACATCAA GGCAGAGCCG
            241 GCCAGCCCTG ACAGTCCAAA GGGTTCCTCG GAGACAGAGA CCGAGCCTCC TGTGGCCCTG
            301 GCCCCTGGTC CAGCTCCCAC TCGCTGCCTC CCAGGCCACA AGGAAGAGGA GGATGGGGAG
            361 GGGGCTGGGC CTGGCGAGCA GGGCGGTGGG AAGCTGGTGC TCAGCTCCCT GCCCAAGCGC
            421 CTCTGCCTGG TCTGTGGGGA CGTGGCCTCC GGCTACCACT ATGGTGTGGC ATCCTGTGAG
            481 GCCTGCAAAG CCTTCTTCAA GAGGACCATC CAGGGGAGCA TCGAGTACAG CTGTCCGGCC
            541 TCCAACGAGT GTGAGATCAC CAAGCGGAGA CGCAAGGCCT GCCAGGCCTG CCGCTTCACC
            601 AAGTGCCTGC GGGTGGGCAT GCTCAAGGAG GGAGTGCGCC TGGACCGCGT CCGGGGTGGG
            661 CGGCAGAAGT ACAAGCGGCG GCCGGAGGTG GACCCACTGC CCTTCCCGGG CCCCTTCCCT
            721 GCTGGGCCCC TGGCAGTCGC TGGAGGCCCC CGGAAGACAG CAGCCCCAGT GAATGCACTG
            781 GTGTCTCATC TGCTGGTGGT TGAGCCTGAG AAGCTCTATG CCATGCCTGA CCCCGCAGGC
            841 CCTGATGGGC ACCTCCCAGC CGTGGCTACC CTCTGTGACC TCTTTGACCG AGAGATTGTG
            901 GTCACCATCA GCTGGGCCAA GAGCATCCCA GGCTTCTCAT CGCTGTCGCT GTCTGACCAG
            961 ATGTCAGTAC TGCAGAGCGT GTGGATGGAG GGTGCTGGTG CTGGTGTGGC CCAGCGCTCA
           1021 CTGCCACTGC AGGATGAGCT GGCCCTTCGC TGAGGACTTA GTCCCTGGGA ATGAAGGAGG
           1081 GGCACGGGCA GGCTGCCTGG GGACCTGGGG GGGCTGCCCT GCTGCAACTA AGTGGCGGGG
          1141 CCGGGCATC
          //
          Primer: BGH_rev2
          Sequence file: RDB12739_A7Jbb.seq check
          >06260_12739_A7Jb_BGH_rev2_B01_02_ABI08.ab1
              1 ACAATAACTA ATGTTGATGA GCACAGCGGG AGGGGCAAAC AACAGATGGC TGGCAACTAG
             61 AAGGCACAGT CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC
            121 CCTCTAGATG CATGCTCGAG CGGCCTCAGT CCATCATGGC CTCGAGCATC TCCAAGAACA
            181 GCTTGTGCAT GGGCACCTTG CCCTCCAGCT TCACCCCATA GAAATGGGCC AGCACTTTGC
            241 CCGCTGTCTG GCGGAGGAGC GGTAGCGTGA GCAGCAGCCT GCCCGCCCGC CGCCGCTCAG
            301 CACCCCCTCC GGGGCCAGCC CGGCCGGCTT CATACTCCAG CAGGGCCTCG TGCAGAGCTT
            361 CTCGCAGCTG CTCCACAGCC TCGGCATCTT CGATGTGCAC AGAGTCTGAA TTGGCAAGGG
            421 CCAAGGCCTT TAGTAGAACA TACTCCTCTC GCTCCAGCCG CAGGGCCTGC AGCCGCCGCA
            481 CTAGTTGCAG CAGGGCAGCC CCCAGTTCCC CCAGGCCAGC TGCCCGTGCC CCCTCTTCAT
            541 CCAGGACTAA GTCCTCAGCG AAGGCCAGCT CATCCTGCAG TGGCAGTGAG CGCTGGGCCA
            601 CACCCAGCAC CAGCACCTCC ATCCACACGC TCTGCAGTAC TGACATCTGG TCAGACAGCG
            661 ACAGCGATGA GAAGCCTGGG ATGCTCTTGG CCCAGCTGAT GGTGACCACA ATCTCTCGGT
            721 CAAAGAGGTC ACAGAGGGTA GCCACGGCTG GGAGGTGCCC ATCAGGGCCT GCGGGGTCAG
            781 GCATGGCATA GAGCTTCTCA GGCTCAACCA CCAGCAGATG AGACACCAGT GCATTCACTG
            841 GGGGCTGCTG TCTTCCGGGG GCCTCCAGCG ACTGCCAGGG GCCCAGCAAG GGAAGGGGCC
            901 CGGGAAGGGC AGTGGGTCCA CCTCCGGCCG CCGCTTGTAC TTCTGCCGCC CACCCCGGGA
            961 CGCGGTCCAG GCGCACTCCC TCCTTGAGCA TGCCCACCCG CAGGCACTTG GTGAAGCGGC
           1021 AGGCCTGGCA AGCCCTTGCG TCTCCGCTTG GTGATCTCAC ACTCGTTGGA GCGGGACAGC
          1081 TGTACTTCGA ATGCTCCCTG AATGATCTCT TGGAAGAAGC TTTTGGCCAA GGCTCA
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB12739_A7Jbc.seq check
          >06260_12739_A7Jb_SV40pro_F_V2_F09_18_ABI24.ab1
              1 TTCAGACGTT TTTTTGGAGG CTAGGCTTTT GCAAAAGCTC CCGGGAGCTT GGATATCCAT
             61 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
            121 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
            181 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
            241 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT
            301 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
            361 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
            421 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
            481 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
            541 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
            601 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC
            661 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT
            721 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
            781 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC
            841 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG
            901 ACTCTGGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CCTGCCATCA CGAGATTTCG
            961 ATTCCACCGC CCGCCCTTTC TATGAAGGTT GGGCTTTCGG AATCGTTTTC CTGGACGCCC
           1021 GGCTGGATGA ATCTCAGCGC GGGGATCTCA TGCTTGGAAG TCATCGCCCA CCCCAACTGT
          1081 TATGGCAGCC TATATTGGTT ACGAATAAGG CAATATAGCC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Igarashi, J., A key role of PGC-1alpha transcriptional coactivator in production of VEGF by a novel angiogenic agent COA-Cl in cultured human fibroblasts. Physiol. Rep. 4 (6). pii: e12742 (2016). PMID 27033444.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl