Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-GFH1

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06202
Resource name pDUAL2-GFH1
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364068.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06202 pDUAL2-GFH1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06202z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14464_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14464_A6L7a.seq check
            >06202_14464_A6L7_nmt1Fw_A05_02_ABI24.ab1
                1 TGGTATTTAT TAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
               61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
              121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
              181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
              241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
              301 CCGGATATCA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA
              361 TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA
              421 ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG
              481 CCAACACTTG TCACTACTTT CTGTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC
              541 ATGAAACAGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GCTATGTACA GGAAAGAACT
              601 ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT
              661 ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT
              721 GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA
              781 AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA
              841 CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC
              901 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC
              961 ATGGGTCCTT CTTTGAGTTT GTAACAGCTG CTGGGATTTA CACATGGCAT GGATGAACTA
             1021 TACAAACCCA ATGGGACTAC AGACGACGAT GACCAACATT CAACACCATT CACACTGAGA
            1081 TCTCGAACTC TAGAGGGAAT CGGCGCCGCC CACCTTTTCT TAATGAG
            //
            Primer: ADH_rev
            Sequence file: RDB14464_A6L7b.seq check
            >06202_14464_A6L7_ADH_rev_B05_05_ABI24.ab1
                1 CGGGGATTCT CAAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
              121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG
              181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG TTTGTATAGT TCATCCATGC CATGTGTAAT
              241 CCCAGCAGCT GTTACAAACT CAAGAAGGAC CATGTGGTCT CTCTTTTCGT TGGGATCTTT
              301 CGAAAGGGCA GATTGTGTGG ACAGGTAATG GTTGTCTGGT AAAAGGACAG GGCCATCGCC
              361 AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG CTTCCATCTT CAATGTTGTG
              421 TCTAATTTTG AAGTTAACTT TGATTCCATT CTTTTGTTTG TCTGCCATGA TGTATACATT
              481 GTGTGAGTTA TAGTTGTATT CCAATTTGTG TCCAAGAATG TTTCCATCTT CTTTAAAATC
              541 AATACCTTTT AACTCGATTC TATTAACAAG GGTATCACCT TCAAACTTGA CTTCAGCACG
              601 TGTCTTGTAG TTCCCGTCAT CTTTGAAAAA TATAGTTCTT TCCTGTACAT AGCCTTCGGG
              661 CATGGCACTC TTGAAAAAGT CATGCTGTTT CATGTGATCT GGGTATCTTG AAAAGCATTG
              721 AACACCATAA CAGAAAGTAG TGACAAGTGT TGGCCACGGA ACAGGTAGTT TTCCAGTAGT
              781 GCAAATAAAT TTAAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA
              841 CAGAAAATTT GTGCCCATTA ACATCACCCA TCTAATTCAA CAAGAATTGG GACACTCCAG
              901 TGAAAGTTCC TTCCTCCTTT ACTCATGATA TCCCGGATCC AGGCCCTGTC GACCGCTAGC
              961 TTTAACAAAG CGACTATAAG TCAGAAAGTG AGATGGAGAT TGAAAATAAT TTAATTCCAA
             1021 CTTATCATGA TGGAATATGG CCAGGAATCC TCTCCTTATA GATCCTTCCA TGATTCGGAT
            1081 AACCAGGTCC ATTATCCGGG T
            //
            Primer: ADH-Forward
            Sequence file: RDB14464_A6L7c.seq check
            >06202_14464_A6L7_ADH-Forward_C05_08_ABI24.ab1
                1 TGGTCATTTA TTGGACACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
               61 TAGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT
              121 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG
              181 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG
              241 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT
              301 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG
              361 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT
              421 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACCTTC CAATTCATTA AATCGAGTCC
              481 TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA
              541 TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT
              601 TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA
              661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA
              721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAT GGGCAAGCGA GACAGAATGG
              781 GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAAATTT GTATTTTTTG
              841 TAATTTTATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA CTTTCAACGA
              901 ATGTCAAAAA TAAGCTCAGC AAAAATATAC AATTTTAGGA AAGGCGATCA GCCACTATCT
              961 CCGTGTAGAT TCTTTTCCTC ATAATTCAAC ATGTACTGCG ACTGAATTTT CTCTAGATGC
            1021 TATAAATTGA ACTATGCATT ACATACCTCA GGTTGTGCAT
            //
            Primer: f1ori_R3
            Sequence file: RDB14464_A6L7d.seq check
            >06202_14464_A6L7_f1ori_R3_D05_11_ABI24.ab1
                1 GGCATGCGCT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
               61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
              121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
              181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG
              241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC
              301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG
              361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT
              421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT
              481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG
              541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG
              601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT
              661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC
              721 AATTTTATAA TCAGGAAGTT AAGTGAATGG TAGCACATGT CGTAAAAATT GTGAATTTTT
              781 ATTGAATAAT ATTTTAAATA CAAGCCTTTC TAGACTAGGT ATACTCATAA ACATATGAGC
              841 AAAAGGATAG AGGAGATTAC ATTTGCATTC TTTCTAACAA ATTTTATTTT ATTGCCCTTT
              901 ACTGGAAAAT TTAAATAATG AGTACTAAAT GAATAAAAAA GCGCCTCAGT ACAAAGAAAA
              961 GATTGCAAAA TTATTGCATT CTTCAATGAA TCAAGTTGCA TATAAGCATA ATTGGAGGTT
            1021 ATTAGTTACT GAACAGGCAG TAGCTAGATT ATTGGCAT
            //
            Primer: Reverse2
            Sequence file: RDB14464_A6L7e.seq check
            >06202_14464_A6L7_Reverse2_E05_14_ABI24.ab1
                1 CGTAAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
               61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
              121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
              181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
              241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
              301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
              361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
              421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
              481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
              541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
              601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
              661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
              721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
              781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT
              841 TGAGGCCTTG TATAATACCC TCGCCTGGCA CTGTATGGCA ATTTGTGATA TGAGCCCAAG
              901 AAGCAATTTT GTACACACCA GATGCATATT GTAGCTTGAC GGTATTTCCC AATGTCTGCG
              961 AAATTTGCGA TCCCTCAAGA TAAGAAAACG ATGCTTTTTT ACCCTAAAGC CACCAGTTTT
             1021 TTCTAACCAT ATCCCTGCTC GGAATTCCTT CGACCACGTT CGAATATTGC TATCTGATAC
            1081 ACAGGAACTT AGGTCCAAAT TTTTAATTC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl