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pDUAL2-GFH1

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06202
Resource name pDUAL2-GFH1
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter.
Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364068.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06202 pDUAL2-GFH1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06202z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14464_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14464_A6L7a.seq
>06202_14464_A6L7_nmt1Fw_A05_02_ABI24.ab1
    1 TGGTATTTAT TAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
  181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
  241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
  301 CCGGATATCA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA
  361 TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA
  421 ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG
  481 CCAACACTTG TCACTACTTT CTGTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC
  541 ATGAAACAGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GCTATGTACA GGAAAGAACT
  601 ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT
  661 ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT
  721 GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA
  781 AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA
  841 CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC
  901 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC
  961 ATGGGTCCTT CTTTGAGTTT GTAACAGCTG CTGGGATTTA CACATGGCAT GGATGAACTA
 1021 TACAAACCCA ATGGGACTAC AGACGACGAT GACCAACATT CAACACCATT CACACTGAGA
1081 TCTCGAACTC TAGAGGGAAT CGGCGCCGCC CACCTTTTCT TAATGAG
//
Primer: ADH_rev RDB14464_A6L7b.seq
>06202_14464_A6L7_ADH_rev_B05_05_ABI24.ab1
    1 CGGGGATTCT CAAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
  121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG
  181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG TTTGTATAGT TCATCCATGC CATGTGTAAT
  241 CCCAGCAGCT GTTACAAACT CAAGAAGGAC CATGTGGTCT CTCTTTTCGT TGGGATCTTT
  301 CGAAAGGGCA GATTGTGTGG ACAGGTAATG GTTGTCTGGT AAAAGGACAG GGCCATCGCC
  361 AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG CTTCCATCTT CAATGTTGTG
  421 TCTAATTTTG AAGTTAACTT TGATTCCATT CTTTTGTTTG TCTGCCATGA TGTATACATT
  481 GTGTGAGTTA TAGTTGTATT CCAATTTGTG TCCAAGAATG TTTCCATCTT CTTTAAAATC
  541 AATACCTTTT AACTCGATTC TATTAACAAG GGTATCACCT TCAAACTTGA CTTCAGCACG
  601 TGTCTTGTAG TTCCCGTCAT CTTTGAAAAA TATAGTTCTT TCCTGTACAT AGCCTTCGGG
  661 CATGGCACTC TTGAAAAAGT CATGCTGTTT CATGTGATCT GGGTATCTTG AAAAGCATTG
  721 AACACCATAA CAGAAAGTAG TGACAAGTGT TGGCCACGGA ACAGGTAGTT TTCCAGTAGT
  781 GCAAATAAAT TTAAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA
  841 CAGAAAATTT GTGCCCATTA ACATCACCCA TCTAATTCAA CAAGAATTGG GACACTCCAG
  901 TGAAAGTTCC TTCCTCCTTT ACTCATGATA TCCCGGATCC AGGCCCTGTC GACCGCTAGC
  961 TTTAACAAAG CGACTATAAG TCAGAAAGTG AGATGGAGAT TGAAAATAAT TTAATTCCAA
 1021 CTTATCATGA TGGAATATGG CCAGGAATCC TCTCCTTATA GATCCTTCCA TGATTCGGAT
1081 AACCAGGTCC ATTATCCGGG T
//
Primer: ADH-Forward RDB14464_A6L7c.seq
>06202_14464_A6L7_ADH-Forward_C05_08_ABI24.ab1
    1 TGGTCATTTA TTGGACACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
   61 TAGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT
  121 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG
  181 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG
  241 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT
  301 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG
  361 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT
  421 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACCTTC CAATTCATTA AATCGAGTCC
  481 TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA
  541 TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT
  601 TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA
  661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA
  721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAT GGGCAAGCGA GACAGAATGG
  781 GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAAATTT GTATTTTTTG
  841 TAATTTTATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA CTTTCAACGA
  901 ATGTCAAAAA TAAGCTCAGC AAAAATATAC AATTTTAGGA AAGGCGATCA GCCACTATCT
  961 CCGTGTAGAT TCTTTTCCTC ATAATTCAAC ATGTACTGCG ACTGAATTTT CTCTAGATGC
1021 TATAAATTGA ACTATGCATT ACATACCTCA GGTTGTGCAT
//
Primer: f1ori_R3 RDB14464_A6L7d.seq
>06202_14464_A6L7_f1ori_R3_D05_11_ABI24.ab1
    1 GGCATGCGCT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
   61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
  121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
  181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG
  241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC
  301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG
  361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT
  421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT
  481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG
  541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG
  601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT
  661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC
  721 AATTTTATAA TCAGGAAGTT AAGTGAATGG TAGCACATGT CGTAAAAATT GTGAATTTTT
  781 ATTGAATAAT ATTTTAAATA CAAGCCTTTC TAGACTAGGT ATACTCATAA ACATATGAGC
  841 AAAAGGATAG AGGAGATTAC ATTTGCATTC TTTCTAACAA ATTTTATTTT ATTGCCCTTT
  901 ACTGGAAAAT TTAAATAATG AGTACTAAAT GAATAAAAAA GCGCCTCAGT ACAAAGAAAA
  961 GATTGCAAAA TTATTGCATT CTTCAATGAA TCAAGTTGCA TATAAGCATA ATTGGAGGTT
1021 ATTAGTTACT GAACAGGCAG TAGCTAGATT ATTGGCAT
//
Primer: Reverse2 RDB14464_A6L7e.seq
>06202_14464_A6L7_Reverse2_E05_14_ABI24.ab1
    1 CGTAAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
  721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
  781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT
  841 TGAGGCCTTG TATAATACCC TCGCCTGGCA CTGTATGGCA ATTTGTGATA TGAGCCCAAG
  901 AAGCAATTTT GTACACACCA GATGCATATT GTAGCTTGAC GGTATTTCCC AATGTCTGCG
  961 AAATTTGCGA TCCCTCAAGA TAAGAAAACG ATGCTTTTTT ACCCTAAAGC CACCAGTTTT
 1021 TTCTAACCAT ATCCCTGCTC GGAATTCCTT CGACCACGTT CGAATATTGC TATCTGATAC
1081 ACAGGAACTT AGGTCCAAAT TTTTAATTC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl