Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL2-FFH81

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14491_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14491_A6L7a.seq
>06201_14491_A6L7_nmt1Fw_E04_13_ABI24.ab1     1 TGCAATTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG    61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG   121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG   181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC   241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCGGATA   301 TCATGGATTA TAAGGATGAT GACGATAAAC CCATGGACTA CAAGGACGAC GATGACAAAC   361 ATCACCACCA TCACCACTGA GATCTCGACT CTAGAGGATC CGGCGCGCCA CTTCTAAATA   421 AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA GTTATAAAAA AAATAAGTGT   481 ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA TTCTTATTCT TGAGTAACTC   541 TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG GTCGCTCTTA TTGACCACAC   601 CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC TAGTGCCTCT ATTGATGCCT   661 ATGGAACCCC TTTGACTGAC GAGACTGTGA AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT   721 TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT GTCGTCCTGA GCAAGGTTTA   781 TTGAAGCTTC GTAAGAGTAT GGGGTGTTTG GGGCCACCTT TCGACCTTGC AACTTTGCCA   841 GCAAGTCTTT AGTCAAGTAC AGCCCTTTGA AGCCTGAATC GTGAGTGTCG ATTTTTTTGT   901 GTTTGTACGA GACTTACTGG AGTGTACTTG GTGAGCGCAC TGAAGACACC GATCCGGTTA   961 TGCTATGACA CTGCTTACAG TTTGGAGAGT ACTCGGTATG CTCGATAGCG ACGCGGGTCT 1021 ACTTCAATCA TATCGAGTTC TCATC //
Primer: ADH-Forward Sequence file: RDB14491_A6L7b.seq
>06201_14491_A6L7_ADH-Forward_F04_16_ABI24.ab1     1 TACTGCCTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA    61 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG   121 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC   181 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC   241 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG   301 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA   361 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT   421 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACCTTCCA ATTCATTAAA TCGAGTCCTC   481 CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT AAGAGAAGTT GTTAGTGATT   541 TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT CTTCTCCATT GTTTCCTTTC   601 TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT TCTGATTATT TTTTTCTATA   661 GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA ATCGGGGGTA TGGTTAGTAG   721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG   781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAAATTT GTATTTTTTT   841 GTAATTTTAT TTCTCATTGT TTTACTTTAA AATGAAATGC GAATTAGAGA AAACTTTCAA   901 CGAAATGTCA AAATAAGCTC AGCAAAAATA TAACAATTTT TAGGAAAGCG ATCAGCCAAC   961 TATCTCGTTG TAGATTCCTT TTCCTCATTA ATCAACAATG TACTGGCGAT GATTTTTCTC 1021 TAGATGCCTA ATAAATTGAA CTAATGCATA TATACCACAG GTTCTGTA //
Primer: f1ori_R3 Sequence file: RDB14491_A6L7c.seq
>06201_14491_A6L7_f1ori_R3_G04_19_ABI24.ab1     1 GGATGGGGCT TGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA    61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT   121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA   181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA   241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA   301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT   361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC   421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT   481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA   541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG   601 GCCCGAGTCT AACTCCTTAA CCACTCGGAC ATAGTGACTT ATCTGACACT CATTGTAAAT   661 TAATATATAT ATAGGCATTT TGTTTAGTTA AAGGTACTTA AGTAATTAGT ATAAACGAAC   721 CAATTTTATA ATCAGGAAGT TAAGTGAATG GTAGCACATG TCGTAAAAAT TGTGAATTTT   781 TATTGAATAA TATTTTAAAT ACAAGCCTTT CTAGACTAGG TATACTCATA AACATATGAG   841 CAAAAGATAG AGGAGATTAC ATTGCATCTT CTACAAATTT ATTTTATTGC CTTTACTGAA   901 AAATTAAATA ATGAGTACTA AAATGATAAA AAGCGCTCAG TACAAGAAAG ATGGCCAAAA   961 TATGCATCCT CATGATTAAA GTGCCATATA GCCATATGGA AGTATAGGTA CTAAACAGCA 1021 GTAGCGAAAT CATGATTATC GCAAGACATG GAAACTGACT TAAGATGTTA //
Primer: Reverse2 Sequence file: RDB14491_A6L7d.seq
>06201_14491_A6L7_Reverse2_H04_22_ABI24.ab1     1 CGGGAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG    61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT   121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT   181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA   241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT   301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG   361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA   421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG   481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT   541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG   601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG   661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT   721 CGGTATGCTT CTCAAACCAT TCTAAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC   781 CTTTGGGAAG ACATTTCAGC CAAAAGCAAG AGACCACGTC CCAAAGTAAA CCAACTTCTT   841 TGAGCCTGTA TAATACCCTC CGCTGCACTG TATGGCAATT TGTGATATGA GCCAGAGCAT   901 TTTGTACACA CCAGATGCAT ATGGTAGCTG GACGTATTTC ATGTCTGCGA CTTGCGATCC   961 TCAAGGATAG AAACGATGCT TTTAACTAGC ACAGTTTTCT ACGATTCTGG TCGAATCTGA 1021 CACGTCAAGT GCTGTACGGA CAATGGTCCA ATCTTAAGTC A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl