RDB06201 - pDUAL2-FFH81

Resource data sheet
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pDUAL2-FFH81

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter

Catalog number	RDB06201
Resource name	pDUAL2-FFH81
Clone info.	S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone	pDUAL2 (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364067.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06201	pDUAL2-FFH81	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06201z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14491_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw	RDB14491_A6L7a.seq
>06201_14491_A6L7_nmt1Fw_E04_13_ABI24.ab1 1 TGCAATTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG 61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG 121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG 181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC 241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCGGATA 301 TCATGGATTA TAAGGATGAT GACGATAAAC CCATGGACTA CAAGGACGAC GATGACAAAC 361 ATCACCACCA TCACCACTGA GATCTCGACT CTAGAGGATC CGGCGCGCCA CTTCTAAATA 421 AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA GTTATAAAAA AAATAAGTGT 481 ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA TTCTTATTCT TGAGTAACTC 541 TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG GTCGCTCTTA TTGACCACAC 601 CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC TAGTGCCTCT ATTGATGCCT 661 ATGGAACCCC TTTGACTGAC GAGACTGTGA AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT 721 TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT GTCGTCCTGA GCAAGGTTTA 781 TTGAAGCTTC GTAAGAGTAT GGGGTGTTTG GGGCCACCTT TCGACCTTGC AACTTTGCCA 841 GCAAGTCTTT AGTCAAGTAC AGCCCTTTGA AGCCTGAATC GTGAGTGTCG ATTTTTTTGT 901 GTTTGTACGA GACTTACTGG AGTGTACTTG GTGAGCGCAC TGAAGACACC GATCCGGTTA 961 TGCTATGACA CTGCTTACAG TTTGGAGAGT ACTCGGTATG CTCGATAGCG ACGCGGGTCT 1021 ACTTCAATCA TATCGAGTTC TCATC //
Primer: ADH-Forward	RDB14491_A6L7b.seq
>06201_14491_A6L7_ADH-Forward_F04_16_ABI24.ab1 1 TACTGCCTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA 61 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG 121 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC 181 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC 241 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG 301 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA 361 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT 421 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACCTTCCA ATTCATTAAA TCGAGTCCTC 481 CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT AAGAGAAGTT GTTAGTGATT 541 TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT CTTCTCCATT GTTTCCTTTC 601 TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT TCTGATTATT TTTTTCTATA 661 GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA ATCGGGGGTA TGGTTAGTAG 721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG 781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAAATTT GTATTTTTTT 841 GTAATTTTAT TTCTCATTGT TTTACTTTAA AATGAAATGC GAATTAGAGA AAACTTTCAA 901 CGAAATGTCA AAATAAGCTC AGCAAAAATA TAACAATTTT TAGGAAAGCG ATCAGCCAAC 961 TATCTCGTTG TAGATTCCTT TTCCTCATTA ATCAACAATG TACTGGCGAT GATTTTTCTC 1021 TAGATGCCTA ATAAATTGAA CTAATGCATA TATACCACAG GTTCTGTA //
Primer: f1ori_R3	RDB14491_A6L7c.seq
>06201_14491_A6L7_f1ori_R3_G04_19_ABI24.ab1 1 GGATGGGGCT TGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT 121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA 181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA 241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA 301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT 361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC 421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT 481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA 541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG 601 GCCCGAGTCT AACTCCTTAA CCACTCGGAC ATAGTGACTT ATCTGACACT CATTGTAAAT 661 TAATATATAT ATAGGCATTT TGTTTAGTTA AAGGTACTTA AGTAATTAGT ATAAACGAAC 721 CAATTTTATA ATCAGGAAGT TAAGTGAATG GTAGCACATG TCGTAAAAAT TGTGAATTTT 781 TATTGAATAA TATTTTAAAT ACAAGCCTTT CTAGACTAGG TATACTCATA AACATATGAG 841 CAAAAGATAG AGGAGATTAC ATTGCATCTT CTACAAATTT ATTTTATTGC CTTTACTGAA 901 AAATTAAATA ATGAGTACTA AAATGATAAA AAGCGCTCAG TACAAGAAAG ATGGCCAAAA 961 TATGCATCCT CATGATTAAA GTGCCATATA GCCATATGGA AGTATAGGTA CTAAACAGCA 1021 GTAGCGAAAT CATGATTATC GCAAGACATG GAAACTGACT TAAGATGTTA //
Primer: Reverse2	RDB14491_A6L7d.seq
>06201_14491_A6L7_Reverse2_H04_22_ABI24.ab1 1 CGGGAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG 61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT 121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT 181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA 241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT 301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG 361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA 421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG 481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT 541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG 601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG 661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT 721 CGGTATGCTT CTCAAACCAT TCTAAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC 781 CTTTGGGAAG ACATTTCAGC CAAAAGCAAG AGACCACGTC CCAAAGTAAA CCAACTTCTT 841 TGAGCCTGTA TAATACCCTC CGCTGCACTG TATGGCAATT TGTGATATGA GCCAGAGCAT 901 TTTGTACACA CCAGATGCAT ATGGTAGCTG GACGTATTTC ATGTCTGCGA CTTGCGATCC 961 TCAAGGATAG AAACGATGCT TTTAACTAGC ACAGTTTTCT ACGATTCTGG TCGAATCTGA 1021 CACGTCAAGT GCTGTACGGA CAATGGTCCA ATCTTAAGTC A //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl