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pDUAL2-FFH41

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06200
Resource name pDUAL2-FFH41
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter.
Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364066.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06200 pDUAL2-FFH41 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06200z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14490_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14490_A6L7a.seq
>06200_14490_A6L7_nmt1Fw_A04_01_ABI24.ab1
    1 TGCAATTTAT TAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
   61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
  121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATAAA
  181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
  241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCGGA
  301 TATCATGGAT TATAAGGATG ATGACGATAA ACCCATGGAC TACAAGGACG ACGATGACAA
  361 ACATCACCAC CATCACCACT GAGATCTCGA CTCTAGAGGA TCCGGCGCGC CACTTCTAAA
  421 TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT AAGTTATAAA AAAAATAAGT
  481 GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA AATTCTTATT CTTGAGTAAC
  541 TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGTATG AGGTCGCTCT TATTGACCAC
  601 ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT ACTAGTGCCT CTATTGATGC
  661 CTATGGAACC CCTTTGACTG ACGAGACTGT GAAGGCTTGT TTGGAAGCTG ACGGTGTTCT
  721 TTTGGGTGCC GTTGGTGGTC CTGAATGGAC CAACCCCAAT TGTCGTCCTG AGCAAGGTTT
  781 ATTGAAGCTT CGTAAGAGTA TGGGTGTTTG GGCCCAACCC TTCGACCTTG CAACTTTGCC
  841 AGCAAGTCTT TAGTCAAGTA CAGCCCCTTT GAAGCCTGAA ATCGTTGAGG TGTCGATTTT
  901 TGTGTGTACG AGACTTACTG GAAGTGTACT TGGTGAGCGC ACTGAGACAC CGAATCGGTA
  961 TGCTATGACA CTGCCTACAG GTGAGGAGTT TCTCGATGCT CGATGCACGC GGGCCACTCA
1021 TCATATCGAT CTCATCGATG ATAAGGTTTG CATAGAATCT
//
Primer: ADH-Forward RDB14490_A6L7b.seq
>06200_14490_A6L7_ADH-Forward_B04_04_ABI24.ab1
    1 TTCTCATTAA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
   61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
  121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
  181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
  241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
  301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
  361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
  421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
  481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
  541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
  601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT
  661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG
  721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG
  781 ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTTGT
  841 AATTTTATTT CTCATTGTTT TACTTTAAAA TGAAATGCGA AATTAGAGAA AACTTTCAAC
  901 GAAATGTCAA AATAAGCTCA GCAAAATATA CAATTTTAGG GAAAGCGATC AGCAACTTAT
  961 TCTCCGTGTA GATTCTTTTT TCTTCATTAA ACTCGACATG TAACTGCCCA ACTGAATTTT
 1021 TCTCTAGATG CTTAATAAAT TGAACTATGA ATATATAGCC AGTTGATAAT GTAACCTCTA
1081 A
//
Primer: f1ori_R3 RDB14490_A6L7c.seq
>06200_14490_A6L7_f1ori_R3_C04_07_ABI24.ab1
    1 GGCAGTGCGG CTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
  241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
  301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
  361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
  421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
  481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
  541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
  601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA
  661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA
  721 CCAATTTTAT AATCAGGAAG TTAAGTGAAT GGTAGCACAT GTCGTAAAAA TTGTGAATTT
  781 TTATTGAATA ATATTTTAAA TACAAGCCTT TCTAGACTAG GTATACTCAT AAACATATGA
  841 GCAAAAGGAT AGAGGAGATT ACATTGCATC TTCTACAAAT TTATTTATTG CCCTTTACTG
  901 AAAAATTAAA TAATGAGTAC TAAATGATAA AAAAGCGCTC AGTACAAGAA AGATTGCAAA
  961 AAATATTGCA TTCTTCATGA ATTAAAGGTG CATATACGCA TATTGAAGGT ATTAGTTACT
 1021 AAACAGGCAG GTTAGCGAAA TGTTAGCATT ATATCGCAGA ACATGTGACA GTAATAAAAA
1081 CTG
//
Primer: Reverse2 RDB14490_A6L7d.seq
>06200_14490_A6L7_Reverse2_D04_10_ABI24.ab1
    1 CAGGCATGAT TACGCCAAGC TTGTGATATT GACGAAACTT TTTGACATCT AATTTATTCT
   61 GTTCCAACAC CAATGTTTAT AACCAAGTTT TATCTTGTTT GTCTACATGG TATTTTACAT
  121 TCATCTACAT ACATCTTTCA TTGGCTTTGT ACATAGTTAT CATTACAAGT CTAAAAAAAT
  181 TCACTCTTTT CTTATTCAAT GTCAATCCAA GAGAAAAGAT TGTGGTAATG TTGTAGGAGC
  241 ATGTTTAATA AATTACTATA GCAAATTACT TTTTATTCCC AAGGTGTTTA TCTATAATAG
  301 TTAATATTTT AGTCGCTACA TAAAATTTTA CCAAAGAGTA CTTGTATACT AATTCTAAAT
  361 GCCTTCTGAC ATAAAACGCC TAGGAAAACA AACGCAAACA AGGCATCGAC TTTTTCAATA
  421 ACCAACCAAA AAAATTTTAC ATTAGTCTTT TTTTAATGCT GAGAAAGTCT TTGCTGATAT
  481 GCCTTCCAAC CAGCTTCTCT ATATCTCTTG GCTTCGACAA CAGGATTACG ACCAGCTCCA
  541 TAGACTCCAC GACCAACAAT GATGATGTCG CTACCGCAGT TTACAATCAC TTCTTCAGGA
  601 GTACGATATT GCTGTCCCAG CCCGTCTCCT TTAACATCCA AGCCGATACC AGGGGACATA
  661 GTTATGTAGT CGCTTTGAAG GTTAGGAAAT CGACGACCAG CTATAAAGCC AAAGCAAAAA
  721 TCGGTATGCT TCTCAAACCA TTCTAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
  781 CTTTGGAAGA CATTTCAGCC AAAAGCAGGA GACACGTCCA AAGGTAAACC ACTTCTTTGA
  841 GGCTTGTATA TACCCTCGCT GCACTGTATG GCCATTTGTG ATATGAGCCC AGAGCATTTT
  901 GTACAACCAG ATGCATATTT GTAGCCTGGA CGTAATTGCA TGTTCTGGCA ATGCCGAATC
961 TCAGATAGGA CGATGCTTAC CTAGGCACAG TTTCACGAAT CCTGTCGAAT C
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl