S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter
Clone info. | S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter. |
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Comment | Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible. |
Vector backbone | pDUAL2 (RDB06192) (Plasmid) |
Selectable markers | Amp^r |
Reference of insert sequence | AB364066.1 (DDBJ accession number) |
Depositor|Developer | Yoshida, Minoru | Matsuyama, Akihisa | |
Sequence | RDB06200z.seq |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB06200 | pDUAL2-FFH41 | DNA solution |
Materials & Methods section:
The pDUAL2-FFH41 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06200). |
Reference section:
Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP] |
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14490_A6L7p1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: nmt1Fw Sequence file: RDB14490_A6L7a.seq ![]() |
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>06200_14490_A6L7_nmt1Fw_A04_01_ABI24.ab1 1 TGCAATTTAT TAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA 61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC 121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATAAA 181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT 241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCGGA 301 TATCATGGAT TATAAGGATG ATGACGATAA ACCCATGGAC TACAAGGACG ACGATGACAA 361 ACATCACCAC CATCACCACT GAGATCTCGA CTCTAGAGGA TCCGGCGCGC CACTTCTAAA 421 TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT AAGTTATAAA AAAAATAAGT 481 GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA AATTCTTATT CTTGAGTAAC 541 TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGTATG AGGTCGCTCT TATTGACCAC 601 ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT ACTAGTGCCT CTATTGATGC 661 CTATGGAACC CCTTTGACTG ACGAGACTGT GAAGGCTTGT TTGGAAGCTG ACGGTGTTCT 721 TTTGGGTGCC GTTGGTGGTC CTGAATGGAC CAACCCCAAT TGTCGTCCTG AGCAAGGTTT 781 ATTGAAGCTT CGTAAGAGTA TGGGTGTTTG GGCCCAACCC TTCGACCTTG CAACTTTGCC 841 AGCAAGTCTT TAGTCAAGTA CAGCCCCTTT GAAGCCTGAA ATCGTTGAGG TGTCGATTTT 901 TGTGTGTACG AGACTTACTG GAAGTGTACT TGGTGAGCGC ACTGAGACAC CGAATCGGTA 961 TGCTATGACA CTGCCTACAG GTGAGGAGTT TCTCGATGCT CGATGCACGC GGGCCACTCA 1021 TCATATCGAT CTCATCGATG ATAAGGTTTG CATAGAATCT // |
Primer: ADH-Forward Sequence file: RDB14490_A6L7b.seq ![]() |
>06200_14490_A6L7_ADH-Forward_B04_04_ABI24.ab1 1 TTCTCATTAA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG 121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT 181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA 241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT 301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC 361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT 421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT 481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT 541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT 601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT 661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG 721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG 781 ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTTGT 841 AATTTTATTT CTCATTGTTT TACTTTAAAA TGAAATGCGA AATTAGAGAA AACTTTCAAC 901 GAAATGTCAA AATAAGCTCA GCAAAATATA CAATTTTAGG GAAAGCGATC AGCAACTTAT 961 TCTCCGTGTA GATTCTTTTT TCTTCATTAA ACTCGACATG TAACTGCCCA ACTGAATTTT 1021 TCTCTAGATG CTTAATAAAT TGAACTATGA ATATATAGCC AGTTGATAAT GTAACCTCTA 1081 A // |
Primer: f1ori_R3 Sequence file: RDB14490_A6L7c.seq ![]() |
>06200_14490_A6L7_f1ori_R3_C04_07_ABI24.ab1 1 GGCAGTGCGG CTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC 121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA 181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG 241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC 301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC 361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT 421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA 481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT 541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG 601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA 661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA 721 CCAATTTTAT AATCAGGAAG TTAAGTGAAT GGTAGCACAT GTCGTAAAAA TTGTGAATTT 781 TTATTGAATA ATATTTTAAA TACAAGCCTT TCTAGACTAG GTATACTCAT AAACATATGA 841 GCAAAAGGAT AGAGGAGATT ACATTGCATC TTCTACAAAT TTATTTATTG CCCTTTACTG 901 AAAAATTAAA TAATGAGTAC TAAATGATAA AAAAGCGCTC AGTACAAGAA AGATTGCAAA 961 AAATATTGCA TTCTTCATGA ATTAAAGGTG CATATACGCA TATTGAAGGT ATTAGTTACT 1021 AAACAGGCAG GTTAGCGAAA TGTTAGCATT ATATCGCAGA ACATGTGACA GTAATAAAAA 1081 CTG // |
Primer: Reverse2 Sequence file: RDB14490_A6L7d.seq ![]() |
>06200_14490_A6L7_Reverse2_D04_10_ABI24.ab1 1 CAGGCATGAT TACGCCAAGC TTGTGATATT GACGAAACTT TTTGACATCT AATTTATTCT 61 GTTCCAACAC CAATGTTTAT AACCAAGTTT TATCTTGTTT GTCTACATGG TATTTTACAT 121 TCATCTACAT ACATCTTTCA TTGGCTTTGT ACATAGTTAT CATTACAAGT CTAAAAAAAT 181 TCACTCTTTT CTTATTCAAT GTCAATCCAA GAGAAAAGAT TGTGGTAATG TTGTAGGAGC 241 ATGTTTAATA AATTACTATA GCAAATTACT TTTTATTCCC AAGGTGTTTA TCTATAATAG 301 TTAATATTTT AGTCGCTACA TAAAATTTTA CCAAAGAGTA CTTGTATACT AATTCTAAAT 361 GCCTTCTGAC ATAAAACGCC TAGGAAAACA AACGCAAACA AGGCATCGAC TTTTTCAATA 421 ACCAACCAAA AAAATTTTAC ATTAGTCTTT TTTTAATGCT GAGAAAGTCT TTGCTGATAT 481 GCCTTCCAAC CAGCTTCTCT ATATCTCTTG GCTTCGACAA CAGGATTACG ACCAGCTCCA 541 TAGACTCCAC GACCAACAAT GATGATGTCG CTACCGCAGT TTACAATCAC TTCTTCAGGA 601 GTACGATATT GCTGTCCCAG CCCGTCTCCT TTAACATCCA AGCCGATACC AGGGGACATA 661 GTTATGTAGT CGCTTTGAAG GTTAGGAAAT CGACGACCAG CTATAAAGCC AAAGCAAAAA 721 TCGGTATGCT TCTCAAACCA TTCTAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC 781 CTTTGGAAGA CATTTCAGCC AAAAGCAGGA GACACGTCCA AAGGTAAACC ACTTCTTTGA 841 GGCTTGTATA TACCCTCGCT GCACTGTATG GCCATTTGTG ATATGAGCCC AGAGCATTTT 901 GTACAACCAG ATGCATATTT GTAGCCTGGA CGTAATTGCA TGTTCTGGCA ATGCCGAATC 961 TCAGATAGGA CGATGCTTAC CTAGGCACAG TTTCACGAAT CCTGTCGAAT C // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP] |
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original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP] |
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