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pDUAL2-FFH41 (#RDB06200)

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter

Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (RDB06192) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364066.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06200z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

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RDB06200 pDUAL2-FFH41 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-FFH41 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06200).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14490_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14490_A6L7a.seq check
>06200_14490_A6L7_nmt1Fw_A04_01_ABI24.ab1
    1 TGCAATTTAT TAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
   61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
  121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATAAA
  181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
  241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCGGA
  301 TATCATGGAT TATAAGGATG ATGACGATAA ACCCATGGAC TACAAGGACG ACGATGACAA
  361 ACATCACCAC CATCACCACT GAGATCTCGA CTCTAGAGGA TCCGGCGCGC CACTTCTAAA
  421 TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT AAGTTATAAA AAAAATAAGT
  481 GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA AATTCTTATT CTTGAGTAAC
  541 TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGTATG AGGTCGCTCT TATTGACCAC
  601 ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT ACTAGTGCCT CTATTGATGC
  661 CTATGGAACC CCTTTGACTG ACGAGACTGT GAAGGCTTGT TTGGAAGCTG ACGGTGTTCT
  721 TTTGGGTGCC GTTGGTGGTC CTGAATGGAC CAACCCCAAT TGTCGTCCTG AGCAAGGTTT
  781 ATTGAAGCTT CGTAAGAGTA TGGGTGTTTG GGCCCAACCC TTCGACCTTG CAACTTTGCC
  841 AGCAAGTCTT TAGTCAAGTA CAGCCCCTTT GAAGCCTGAA ATCGTTGAGG TGTCGATTTT
  901 TGTGTGTACG AGACTTACTG GAAGTGTACT TGGTGAGCGC ACTGAGACAC CGAATCGGTA
  961 TGCTATGACA CTGCCTACAG GTGAGGAGTT TCTCGATGCT CGATGCACGC GGGCCACTCA
1021 TCATATCGAT CTCATCGATG ATAAGGTTTG CATAGAATCT
//
Primer: ADH-Forward
Sequence file: RDB14490_A6L7b.seq check
>06200_14490_A6L7_ADH-Forward_B04_04_ABI24.ab1
    1 TTCTCATTAA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
   61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
  121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
  181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
  241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
  301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
  361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
  421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
  481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
  541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
  601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT
  661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG
  721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG
  781 ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTTGT
  841 AATTTTATTT CTCATTGTTT TACTTTAAAA TGAAATGCGA AATTAGAGAA AACTTTCAAC
  901 GAAATGTCAA AATAAGCTCA GCAAAATATA CAATTTTAGG GAAAGCGATC AGCAACTTAT
  961 TCTCCGTGTA GATTCTTTTT TCTTCATTAA ACTCGACATG TAACTGCCCA ACTGAATTTT
 1021 TCTCTAGATG CTTAATAAAT TGAACTATGA ATATATAGCC AGTTGATAAT GTAACCTCTA
1081 A
//
Primer: f1ori_R3
Sequence file: RDB14490_A6L7c.seq check
>06200_14490_A6L7_f1ori_R3_C04_07_ABI24.ab1
    1 GGCAGTGCGG CTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
  241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
  301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
  361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
  421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
  481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
  541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
  601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA
  661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA
  721 CCAATTTTAT AATCAGGAAG TTAAGTGAAT GGTAGCACAT GTCGTAAAAA TTGTGAATTT
  781 TTATTGAATA ATATTTTAAA TACAAGCCTT TCTAGACTAG GTATACTCAT AAACATATGA
  841 GCAAAAGGAT AGAGGAGATT ACATTGCATC TTCTACAAAT TTATTTATTG CCCTTTACTG
  901 AAAAATTAAA TAATGAGTAC TAAATGATAA AAAAGCGCTC AGTACAAGAA AGATTGCAAA
  961 AAATATTGCA TTCTTCATGA ATTAAAGGTG CATATACGCA TATTGAAGGT ATTAGTTACT
 1021 AAACAGGCAG GTTAGCGAAA TGTTAGCATT ATATCGCAGA ACATGTGACA GTAATAAAAA
1081 CTG
//
Primer: Reverse2
Sequence file: RDB14490_A6L7d.seq check
>06200_14490_A6L7_Reverse2_D04_10_ABI24.ab1
    1 CAGGCATGAT TACGCCAAGC TTGTGATATT GACGAAACTT TTTGACATCT AATTTATTCT
   61 GTTCCAACAC CAATGTTTAT AACCAAGTTT TATCTTGTTT GTCTACATGG TATTTTACAT
  121 TCATCTACAT ACATCTTTCA TTGGCTTTGT ACATAGTTAT CATTACAAGT CTAAAAAAAT
  181 TCACTCTTTT CTTATTCAAT GTCAATCCAA GAGAAAAGAT TGTGGTAATG TTGTAGGAGC
  241 ATGTTTAATA AATTACTATA GCAAATTACT TTTTATTCCC AAGGTGTTTA TCTATAATAG
  301 TTAATATTTT AGTCGCTACA TAAAATTTTA CCAAAGAGTA CTTGTATACT AATTCTAAAT
  361 GCCTTCTGAC ATAAAACGCC TAGGAAAACA AACGCAAACA AGGCATCGAC TTTTTCAATA
  421 ACCAACCAAA AAAATTTTAC ATTAGTCTTT TTTTAATGCT GAGAAAGTCT TTGCTGATAT
  481 GCCTTCCAAC CAGCTTCTCT ATATCTCTTG GCTTCGACAA CAGGATTACG ACCAGCTCCA
  541 TAGACTCCAC GACCAACAAT GATGATGTCG CTACCGCAGT TTACAATCAC TTCTTCAGGA
  601 GTACGATATT GCTGTCCCAG CCCGTCTCCT TTAACATCCA AGCCGATACC AGGGGACATA
  661 GTTATGTAGT CGCTTTGAAG GTTAGGAAAT CGACGACCAG CTATAAAGCC AAAGCAAAAA
  721 TCGGTATGCT TCTCAAACCA TTCTAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
  781 CTTTGGAAGA CATTTCAGCC AAAAGCAGGA GACACGTCCA AAGGTAAACC ACTTCTTTGA
  841 GGCTTGTATA TACCCTCGCT GCACTGTATG GCCATTTGTG ATATGAGCCC AGAGCATTTT
  901 GTACAACCAG ATGCATATTT GTAGCCTGGA CGTAATTGCA TGTTCTGGCA ATGCCGAATC
961 TCAGATAGGA CGATGCTTAC CTAGGCACAG TTTCACGAAT CCTGTCGAAT C
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]