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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-HFG81

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06198
Resource name pDUAL2-HFG81
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364064.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06198 pDUAL2-HFG81 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06198z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14463_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14463_A6L7a.seq check
            >06198_14463_A6L7_nmt1Fw_D03_12_ABI24.ab1
                1 TGCAATTTAT TAAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
               61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
              121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG
              181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC
              241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCTATGC
              301 ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG GGTAAAGGAG
              361 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC
              421 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA
              481 AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC AACACTTGTC ACTACTTTCT
              541 GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT GAAACAGCAT GACTTTTTCA
              601 AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA
              661 ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT
              721 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT
              781 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAAAT CAAAGTTAAC
              841 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA
              901 AATACTCCAA TTTGGCGATG GGCCCTGTCC TTTTACCAGA CACCATTACC TGTCCACAAC
              961 AATCTGCCCT TTCGAAAGAT CCAACGAAAA AGAGAGACCA CATGGTCTTC TTGAGTTTGT
             1021 AACAGCCTGC CTGGGATACA CATGGCCATG GAATGACCTA TACAAAGGAA TCATGAGATC
            1081 TCACTCTAGG AGGATTCGGG CGCGCTCAAT TTCTA
            //
            Primer: ADH_rev
            Sequence file: RDB14463_A6L7b.seq check
            >06198_14463_A6L7_ADH_rev_E03_15_ABI24.ab1
                1 GGGGGGTCTC AGAATAAGAT TTTCGTTTTA AACCTAAGAG TCACTTTAAA ATTTGTATAC
               61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
              121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC ATGATATCTT TGTATAGTTC
              181 ATCCATGCCA TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT
              241 CTTTTCGTTG GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA
              301 AAGGACAGGG CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT
              361 TCCATCTTCA ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC
              421 TGCCATGATG TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT
              481 TCCATCTTCT TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC
              541 AAACTTGACT TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC
              601 CTGTACATAG CCTTCGGGCA TGGCACTCTT GAAAAAGTCA TGCTGTTTCA TGTGATCTGG
              661 GTATCTTGAA AAGCATTGAA CACCATAACA GAAAGTAGTG ACAAGTGTTG GCCACGGAAC
              721 AGGTAGTTTT CCAGTAGTGC AAATAAATTT AAAGGGTAAG TTTTCCGTAT GTTGCATCAC
              781 CCTTCACCCT CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAAATTCAAC
              841 AAGAATTGGG ACAACTCCAG TGAAAAGTTC TTCTCCTTTA CCCATGGGTT TATCGTCATC
              901 ATCCCTTATA ATCCATATGA TGATGGTGGT GATGCATAGA TCCAGGCCTG TCGACGCTAG
              961 GCTTTAACAA AGCGACTTAT AGGTTCAGAA GGTGGAGATG AGATTGCATA TTAATTCAAC
            1021 TATCAACTGG ATGAATTATG CCCA
            //
            Primer: ADH-Forward
            Sequence file: RDB14463_A6L7c.seq check
            >06198_14463_A6L7_ADH-Forward_F03_18_ABI24.ab1
                1 TGGTCTTTTA ATTGACACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
               61 TAGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT
              121 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG
              181 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG
              241 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT
              301 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG
              361 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT
              421 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACCTTC CAATTCATTA AATCGAGTCC
              481 TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA
              541 TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT
              601 TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA
              661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA
              721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG GGGCAAGCGA GACAGAATGG
              781 GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTTG
              841 TAAATTTTAT TTCTCATTGT TTTACTTTAA ATGAATGCGA AATTAGAGAA AACTTCAACG
              901 AAATGTCAAA AATAAGCTCA GCAAAATATA CAATTTTAGG AAGCGATCAG CACTATCTCG
              961 TTGTAGATTC TTTTTCCTTC ATAATTCGAC ATGGTACCTG CGATGATTTT CTCTAGATGC
            1021 CTATAAATGG AACTAATGAA TTCGTACCCA GTTCGATT
            //
            Primer: f1ori_R3
            Sequence file: RDB14463_A6L7d.seq check
            >06198_14463_A6L7_f1ori_R3_G03_21_ABI24.ab1
                1 GGCGTGGCGC ATGCGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
              241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
              301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
              361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
              421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
              481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
              541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
              601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA
              661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA
              721 CCAATTTTAT AATCAGGAAG TTTAAGTGAA TGGGTAGCAC ATGTCGTAAA AATTGTGAAT
              781 TTTTATTGAA TAATATTTTT AAAATACAAG CCTTTTCTAG ACTAGGTATA CTCATAAAAC
              841 ATATGAGCAA AACGATAAGA GGGAGATTAC ATTGCATCTT CTACAAATTT ATTTTATTGC
              901 CCCTTTACTG AAAATAAATA ATGAGTACTA AATGATAAAA GCGCTCAGTA ACAAGAAAGA
              961 TGCCAAAAAT ATGCAATTCC TCAATGATCA AGTGCCAATC AGCCATATTG GAAAGTCAAT
            1021 GTACCTGAAG CAGCCAGTTA GGCCGGA
            //
            Primer: Reverse2
            Sequence file: RDB14463_A6L7e.seq check
            >06198_14463_A6L7_Reverse2_H03_24_ABI24.ab1
                1 CGGGCATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
               61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
              121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
              181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
              241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
              301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
              361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
              421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
              481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
              541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
              601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
              661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
              721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
              781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT
              841 TGAGGCCTTG TATAATACCC TCGCCCTGGC ACTGTATGGC AATTTGTGAT ATGAGCCCAA
              901 GAAGCAATTT TGTACACACC AGATGCATAT TGTAGCTGAC GGTATTTCCC AATGTCTGCG
              961 AATTTGCGAT TCTTCGAGAT TAGAGACGAA TGCTTTTTAA CTAAAGGTCA CAGTTTTTCC
            1021 TAACGTATAT CCTGATCGAA TCCTCGACAA CGGTCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl