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pDUAL2-HFG81

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06198
Resource name pDUAL2-HFG81
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter.
Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364064.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06198 pDUAL2-HFG81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06198z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14463_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14463_A6L7a.seq
>06198_14463_A6L7_nmt1Fw_D03_12_ABI24.ab1
    1 TGCAATTTAT TAAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG
  181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC
  241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCTATGC
  301 ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG GGTAAAGGAG
  361 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC
  421 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA
  481 AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC AACACTTGTC ACTACTTTCT
  541 GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT GAAACAGCAT GACTTTTTCA
  601 AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA
  661 ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT
  721 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT
  781 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAAAT CAAAGTTAAC
  841 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA
  901 AATACTCCAA TTTGGCGATG GGCCCTGTCC TTTTACCAGA CACCATTACC TGTCCACAAC
  961 AATCTGCCCT TTCGAAAGAT CCAACGAAAA AGAGAGACCA CATGGTCTTC TTGAGTTTGT
 1021 AACAGCCTGC CTGGGATACA CATGGCCATG GAATGACCTA TACAAAGGAA TCATGAGATC
1081 TCACTCTAGG AGGATTCGGG CGCGCTCAAT TTCTA
//
Primer: ADH_rev RDB14463_A6L7b.seq
>06198_14463_A6L7_ADH_rev_E03_15_ABI24.ab1
    1 GGGGGGTCTC AGAATAAGAT TTTCGTTTTA AACCTAAGAG TCACTTTAAA ATTTGTATAC
   61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
  121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC ATGATATCTT TGTATAGTTC
  181 ATCCATGCCA TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT
  241 CTTTTCGTTG GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA
  301 AAGGACAGGG CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT
  361 TCCATCTTCA ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC
  421 TGCCATGATG TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT
  481 TCCATCTTCT TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC
  541 AAACTTGACT TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC
  601 CTGTACATAG CCTTCGGGCA TGGCACTCTT GAAAAAGTCA TGCTGTTTCA TGTGATCTGG
  661 GTATCTTGAA AAGCATTGAA CACCATAACA GAAAGTAGTG ACAAGTGTTG GCCACGGAAC
  721 AGGTAGTTTT CCAGTAGTGC AAATAAATTT AAAGGGTAAG TTTTCCGTAT GTTGCATCAC
  781 CCTTCACCCT CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAAATTCAAC
  841 AAGAATTGGG ACAACTCCAG TGAAAAGTTC TTCTCCTTTA CCCATGGGTT TATCGTCATC
  901 ATCCCTTATA ATCCATATGA TGATGGTGGT GATGCATAGA TCCAGGCCTG TCGACGCTAG
  961 GCTTTAACAA AGCGACTTAT AGGTTCAGAA GGTGGAGATG AGATTGCATA TTAATTCAAC
1021 TATCAACTGG ATGAATTATG CCCA
//
Primer: ADH-Forward RDB14463_A6L7c.seq
>06198_14463_A6L7_ADH-Forward_F03_18_ABI24.ab1
    1 TGGTCTTTTA ATTGACACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
   61 TAGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT
  121 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG
  181 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG
  241 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT
  301 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG
  361 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT
  421 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACCTTC CAATTCATTA AATCGAGTCC
  481 TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA
  541 TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT
  601 TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA
  661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA
  721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG GGGCAAGCGA GACAGAATGG
  781 GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTTG
  841 TAAATTTTAT TTCTCATTGT TTTACTTTAA ATGAATGCGA AATTAGAGAA AACTTCAACG
  901 AAATGTCAAA AATAAGCTCA GCAAAATATA CAATTTTAGG AAGCGATCAG CACTATCTCG
  961 TTGTAGATTC TTTTTCCTTC ATAATTCGAC ATGGTACCTG CGATGATTTT CTCTAGATGC
1021 CTATAAATGG AACTAATGAA TTCGTACCCA GTTCGATT
//
Primer: f1ori_R3 RDB14463_A6L7d.seq
>06198_14463_A6L7_f1ori_R3_G03_21_ABI24.ab1
    1 GGCGTGGCGC ATGCGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
  241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
  301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
  361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
  421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
  481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
  541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
  601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA
  661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA
  721 CCAATTTTAT AATCAGGAAG TTTAAGTGAA TGGGTAGCAC ATGTCGTAAA AATTGTGAAT
  781 TTTTATTGAA TAATATTTTT AAAATACAAG CCTTTTCTAG ACTAGGTATA CTCATAAAAC
  841 ATATGAGCAA AACGATAAGA GGGAGATTAC ATTGCATCTT CTACAAATTT ATTTTATTGC
  901 CCCTTTACTG AAAATAAATA ATGAGTACTA AATGATAAAA GCGCTCAGTA ACAAGAAAGA
  961 TGCCAAAAAT ATGCAATTCC TCAATGATCA AGTGCCAATC AGCCATATTG GAAAGTCAAT
1021 GTACCTGAAG CAGCCAGTTA GGCCGGA
//
Primer: Reverse2 RDB14463_A6L7e.seq
>06198_14463_A6L7_Reverse2_H03_24_ABI24.ab1
    1 CGGGCATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
  721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
  781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT
  841 TGAGGCCTTG TATAATACCC TCGCCCTGGC ACTGTATGGC AATTTGTGAT ATGAGCCCAA
  901 GAAGCAATTT TGTACACACC AGATGCATAT TGTAGCTGAC GGTATTTCCC AATGTCTGCG
  961 AATTTGCGAT TCTTCGAGAT TAGAGACGAA TGCTTTTTAA CTAAAGGTCA CAGTTTTTCC
1021 TAACGTATAT CCTGATCGAA TCCTCGACAA CGGTCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl