RDB06198 - pDUAL2-HFG81

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pDUAL2-HFG81 (#RDB06198)

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter

Clone info.	S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter.
Comment	Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone	pDUAL2 (RDB06192) (Plasmid)
Selectable markers	Amp^r
Reference of insert sequence	AB364064.1 (DDBJ accession number)
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06198z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06198	pDUAL2-HFG81	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-HFG81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06198).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14463_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14463_A6L7a.seq
>06198_14463_A6L7_nmt1Fw_D03_12_ABI24.ab1 1 TGCAATTTAT TAAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG 61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG 121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG 181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC 241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCTATGC 301 ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG GGTAAAGGAG 361 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC 421 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA 481 AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC AACACTTGTC ACTACTTTCT 541 GTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCACAT GAAACAGCAT GACTTTTTCA 601 AGAGTGCCAT GCCCGAAGGC TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA 661 ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT 721 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT 781 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAAAT CAAAGTTAAC 841 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA 901 AATACTCCAA TTTGGCGATG GGCCCTGTCC TTTTACCAGA CACCATTACC TGTCCACAAC 961 AATCTGCCCT TTCGAAAGAT CCAACGAAAA AGAGAGACCA CATGGTCTTC TTGAGTTTGT 1021 AACAGCCTGC CTGGGATACA CATGGCCATG GAATGACCTA TACAAAGGAA TCATGAGATC 1081 TCACTCTAGG AGGATTCGGG CGCGCTCAAT TTCTA //
Primer: ADH_rev Sequence file: RDB14463_A6L7b.seq
>06198_14463_A6L7_ADH_rev_E03_15_ABI24.ab1 1 GGGGGGTCTC AGAATAAGAT TTTCGTTTTA AACCTAAGAG TCACTTTAAA ATTTGTATAC 61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA 121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC ATGATATCTT TGTATAGTTC 181 ATCCATGCCA TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT 241 CTTTTCGTTG GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA 301 AAGGACAGGG CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT 361 TCCATCTTCA ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC 421 TGCCATGATG TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT 481 TCCATCTTCT TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC 541 AAACTTGACT TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC 601 CTGTACATAG CCTTCGGGCA TGGCACTCTT GAAAAAGTCA TGCTGTTTCA TGTGATCTGG 661 GTATCTTGAA AAGCATTGAA CACCATAACA GAAAGTAGTG ACAAGTGTTG GCCACGGAAC 721 AGGTAGTTTT CCAGTAGTGC AAATAAATTT AAAGGGTAAG TTTTCCGTAT GTTGCATCAC 781 CCTTCACCCT CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAAATTCAAC 841 AAGAATTGGG ACAACTCCAG TGAAAAGTTC TTCTCCTTTA CCCATGGGTT TATCGTCATC 901 ATCCCTTATA ATCCATATGA TGATGGTGGT GATGCATAGA TCCAGGCCTG TCGACGCTAG 961 GCTTTAACAA AGCGACTTAT AGGTTCAGAA GGTGGAGATG AGATTGCATA TTAATTCAAC 1021 TATCAACTGG ATGAATTATG CCCA //
Primer: ADH-Forward Sequence file: RDB14463_A6L7c.seq
>06198_14463_A6L7_ADH-Forward_F03_18_ABI24.ab1 1 TGGTCTTTTA ATTGACACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC 61 TAGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT 121 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG 181 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG 241 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT 301 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG 361 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT 421 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACCTTC CAATTCATTA AATCGAGTCC 481 TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA 541 TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT 601 TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA 661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA 721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG GGGCAAGCGA GACAGAATGG 781 GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTTG 841 TAAATTTTAT TTCTCATTGT TTTACTTTAA ATGAATGCGA AATTAGAGAA AACTTCAACG 901 AAATGTCAAA AATAAGCTCA GCAAAATATA CAATTTTAGG AAGCGATCAG CACTATCTCG 961 TTGTAGATTC TTTTTCCTTC ATAATTCGAC ATGGTACCTG CGATGATTTT CTCTAGATGC 1021 CTATAAATGG AACTAATGAA TTCGTACCCA GTTCGATT //
Primer: f1ori_R3 Sequence file: RDB14463_A6L7d.seq
>06198_14463_A6L7_f1ori_R3_G03_21_ABI24.ab1 1 GGCGTGGCGC ATGCGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC 121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA 181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG 241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC 301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC 361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT 421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA 481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT 541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG 601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA 661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA 721 CCAATTTTAT AATCAGGAAG TTTAAGTGAA TGGGTAGCAC ATGTCGTAAA AATTGTGAAT 781 TTTTATTGAA TAATATTTTT AAAATACAAG CCTTTTCTAG ACTAGGTATA CTCATAAAAC 841 ATATGAGCAA AACGATAAGA GGGAGATTAC ATTGCATCTT CTACAAATTT ATTTTATTGC 901 CCCTTTACTG AAAATAAATA ATGAGTACTA AATGATAAAA GCGCTCAGTA ACAAGAAAGA 961 TGCCAAAAAT ATGCAATTCC TCAATGATCA AGTGCCAATC AGCCATATTG GAAAGTCAAT 1021 GTACCTGAAG CAGCCAGTTA GGCCGGA //
Primer: Reverse2 Sequence file: RDB14463_A6L7e.seq
>06198_14463_A6L7_Reverse2_H03_24_ABI24.ab1 1 CGGGCATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG 61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT 121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT 181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA 241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT 301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG 361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA 421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG 481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT 541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG 601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG 661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT 721 CGGTATGCTT CTCAAACCAT TCTAAGGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC 781 CTTTGGAAGA CATTTCAGCC AAAAGCAAGA GACCACGTCC CAAAGGTAAA CCAACTTCTT 841 TGAGGCCTTG TATAATACCC TCGCCCTGGC ACTGTATGGC AATTTGTGAT ATGAGCCCAA 901 GAAGCAATTT TGTACACACC AGATGCATAT TGTAGCTGAC GGTATTTCCC AATGTCTGCG 961 AATTTGCGAT TCTTCGAGAT TAGAGACGAA TGCTTTTTAA CTAAAGGTCA CAGTTTTTCC 1021 TAACGTATAT CCTGATCGAA TCCTCGACAA CGGTCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

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