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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-HFG41

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06197
Resource name pDUAL2-HFG41
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364063.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06197 pDUAL2-HFG41 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06197z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14462_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14462_A6L7a.seq check
            >06197_14462_A6L7_nmt1Fw_G02_20_ABI24.ab1
                1 AGCATTTTAT TAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
               61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
              121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAA
              181 AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA
              241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCTA
              301 TGCATCACCA CCATCATCAT ATGGATTATA AGGATGATGA CGATAAACCC ATGGGTAAAG
              361 GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG
              421 GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC
              481 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTT
              541 TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG CATGACTTTT
              601 TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG
              661 GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG
              721 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAAATAC
              781 AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG AATCAAAGTT
              841 TAACTTCAAA ATTAGACACA ACATTGAAGA TGGGAAGCGT TTCAACTAGC AGACCATTAT
              901 CAACAAAAAT ACTCCAATTG GCGATGGGCC CTGTCCTTTT ACCAGACACC ATTACCTGTC
              961 CACACATCTG CCTTTCGAAA GATCCCCACG AAAAAGAAGA GAACACATGG TCTTCTTGAG
             1021 TTTGTAACAG CTGCCTGGGA ATTAACACAT TGGCCATTGA ATGGACTATA CAAGCATTCA
            1081 TGAATCTCGA ACTTCTTAGA GAGGGGACTT CCGGGC
            //
            Primer: ADH_rev
            Sequence file: RDB14462_A6L7b.seq check
            >06197_14462_A6L7_ADH_rev_H02_23_ABI24.ab1
                1 GGGGAGTCTC AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
               61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
              121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT GTATAGTTCA
              181 TCCATGCCAT GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT GTGGTCTCTC
              241 TTTTCGTTGG GATCTTTCGA AAGGGCAGAT TGTGTGGACA GGTAATGGTT GTCTGGTAAA
              301 AGGACAGGGC CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGCTT
              361 CCATCTTCAA TGTTGTGTCT AATTTTGAAG TTAACTTTGA TTCCATTCTT TTGTTTGTCT
              421 GCCATGATGT ATACATTGTG TGAGTTATAG TTGTATTCCA ATTTGTGTCC AAGAATGTTT
              481 CCATCTTCTT TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT ATCACCTTCA
              541 AACTTGACTT CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT AGTTCTTTCC
              601 TGTACATAGC CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCTGTTTCAT GTGATCTGGG
              661 TATCTTGAAA AGCATTGAAC ACCATAACAG AAAGTAGTGA CAAGTGTTGG CCACGGAACA
              721 GGTAGTTTTC CAGTAGTGCA AATAAATTTA AAGGGTAAGT TTTCCGTATG TTGCATCACC
              781 TTCACCCTCT CCACTGACAG AAAATTTGTG CCCATTAAAC ATCACCATCT AATTCAACAA
              841 GAAATTGGGA CAACTCCCAG TGAAAAGTTT CTTCTCCTTT ACCCATGGGG TTTTATCGTC
              901 ATCATCCCTT ATAATCCATT ATGATGATGG TGGTGATGCC ATAGGATCCA AGGGCCTGTC
              961 GACGCTAGGC TTTAACAAAA GCGACCTATA GGTCAGGAAG TGGAGTATGA GATGCAGATT
            1021 AGTCACTATT TCCAATTGGA
            //
            Primer: ADH-Forward
            Sequence file: RDB14462_A6L7c.seq check
            >06197_14462_A6L7_ADH-Forward_A03_03_ABI24.ab1
                1 TAGGCACTTA ATGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
               61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
              121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
              181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
              241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
              301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
              361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
              421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
              481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
              541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
              601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTTCTA
              661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA
              721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAAGTA GGGCAAGCGA GACAGAATGG
              781 GATACAAGGG CATCGTCTAT TAATTATTAG CTAAAAATGT ATTTTAATTT GTATTTTTGT
              841 ATTTTATTTT CTCAATGTTT ACTAAAATGA ATGCGACTAG AAGAAAACTT TCACGAATGT
              901 CAAAAATAAG CTCAGCAAAA TTATACAATT TAAACAAGCG ATCAGCACTT ATTCTCGGTT
              961 GTAGAATTCT TTTCTACAAT AATCGACATG TTACTGGCAA TTGGAATGTC CCTTGATGCC
            1021 TTATAAATGA AGCCTAATG
            //
            Primer: f1ori_R3
            Sequence file: RDB14462_A6L7d.seq check
            >06197_14462_A6L7_f1ori_R3_B03_06_ABI24.ab1
                1 GCGATTGCGG CTTGCGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
               61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
              121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
              181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG
              241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA
              301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT
              361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC
              421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT
              481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT
              541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC
              601 GGGCCCGAGT CTAACTCCTT AACCACTCGG ACATAGTGAC TTATCTGACA CTCATTGTAA
              661 ATTAATATAT ATATAGGCAT TTTGTTTAGT TAAAGGTACT TAAGTAATTA GTATAAACGA
              721 ACCAATTTTA TAATCAGGAA GTTAAGTGAA TGGTAGCACA TGTCGTAAAA ATTGTGAATT
              781 TTTATTGAAT AATATTTTAA ATACAAGCCT TTCTAGACTA GGTATACTCA TAAACATATG
              841 AGCAAAAGGA TAGAGGAGAT TACATTGCAT CTTCTACAAA TTTATTTATT GCCCTTTACT
              901 GAAAAAATTA AATAATGAGT ACTAAATGAT AAAAAGCGCT CAGTACAAGA AAGATTGCAA
              961 AAATATTGCA TTCCTTCATG AAATTAAAGT TGCATATACG CATATTGAAA GTAATAGTAC
             1021 TTAAAACAGC AGTTTAGCGA AAATTAATTA GGATTATATT CGGCAAGACA TGTGACAGTC
            1081 AATTTAAAAA ATTGTAGCAA TTTTTTTTAC
            //
            Primer: Reverse2
            Sequence file: RDB14462_A6L7e.seq check
            >06197_14462_A6L7_Reverse2_C03_09_ABI24.ab1
                1 CAGAATCATT ACGCCAGCTT GTGATATTGA CGAAACTTTT TGACATCTAA TTTATTCTGT
               61 TCCAACACCA ATGTTTATAA CCAAGTTTTA TCTTGTTTGT CTACATGGTA TTTTACATTC
              121 ATCTACATAC ATCTTTCATT GGCTTTGTAC ATAGTTATCA TTACAAGTCT AAAAAAATTC
              181 ACTCTTTTCT TATTCAATGT CAATCCAAGA GAAAAGATTG TGGTAATGTT GTAGGAGCAT
              241 GTTTAATAAA TTACTATAGC AAATTACTTT TTATTCCCAA GGTGTTTATC TATAATAGTT
              301 AATATTTTAG TCGCTACATA AAATTTTACC AAAGAGTACT TGTATACTAA TTCTAAATGC
              361 CTTCTGACAT AAAACGCCTA GGAAAACAAA CGCAAACAAG GCATCGACTT TTTCAATAAC
              421 CAACCAAAAA AATTTTACAT TAGTCTTTTT TTAATGCTGA GAAAGTCTTT GCTGATATGC
              481 CTTCCAACCA GCTTCTCTAT ATCTCTTGGC TTCGACAACA GGATTACGAC CAGCTCCATA
              541 GACTCCACGA CCAACAATGA TGATGTCGCT ACCGCAGTTT ACAATCACTT CTTCAGGAGT
              601 ACGATATTGC TGTCCCAGCC CGTCTCCTTT AACATCCAAG CCGATACCAG GGGACATAGT
              661 TATGTAGTCG CTTTGAAGGT TAGGAAATCG ACGACCAGCT ATAAAGCCAA AGCAAAAATC
              721 GGTATGCTTC TCAAACCATT CTAAGGTTTT CTCTGTGTAG GAACCAGTAG CCAAAGAGCC
              781 TTTGGAAGAC ATTTCAGCCA AAAGCAAGAG ACCACGTCCC AAAGGTAAAC CAACTTCTTT
              841 GAGGCCTTGT ATAATACCCT CGCCCTGGCA CTGTATGGCA AATTTGTGAT ATGAGCCCAA
              901 GAAGCAATTT TGTACACACC AGATGCATAT TGTAGCTTGA CGGTATTTTC AATGTCTGCG
              961 ATTGCGATCC TCAAAGAATA GGAAACCGAT GCCTTTTACT ACGTCACAGT TTTCTAACAT
             1021 ATCTGATTCG AATCCTCGGA CACCGTCGAA TGTGATCTGA ATACTCAGGA ACTAGGGGAT
            1081 CA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl