Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL2-HFG1

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Catalog number RDB06196
Resource name pDUAL2-HFG1
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364062.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06196 pDUAL2-HFG1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06196z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14461_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14461_A6L7a.seq check
            >06196_14461_A6L7_nmt1Fw_B02_05_ABI24.ab1
                1 CGGCATTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT
               61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA
              121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATATAT
              181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
              241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
              301 TATGCATCAC CACCATCATC ATATGGATTA TAAGGATGAT GACGATAAAC CCATGGGTAA
              361 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA
              421 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC
              481 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTGTCACTAC
              541 TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC AGCATGACTT
              601 TTTCAAAGAG TGCCATGCCC GAAGGCTATG TACAGGAAAG AACTATATTT TTCAAAGATG
              661 ACGGGGAACT ACAAGACACG TGCTGAAGTC AAAGTTTTGA AGGTGATACC CTTGTTAATA
              721 GAAATCGAGT TAAAAGGTAT TTGAATTTTA AAGAAAGATG GGAAACATTT CTTGGGACAC
              781 AAATTGGAAT ACAACTATTA ACTCACCACA ATTGTATACC ATCAATGGTC AGACAAAACC
              841 AAAAGAATTG GAATCAAAAG TTAACTTTCC AAAATTGAGT CACAACATTT GAAGAATGGA
              901 AAGCCGTTGC AACCTAGCAA GACCATTTAT CCACCAAAGT ACTTCGAATT GGGCGAATGC
              961 GCCCTGGTAC CTTTTTAACA AAACAACCAT TTTACCCTGA CCCATAGCAA TGCTGGCCAT
            1021 TACAAAAGAT ATCCCAAACG AAAAAAAAAG CACTACTGAG GTC
            //
            Primer: ADH_rev
            Sequence file: RDB14461_A6L7b.seq check
            >06196_14461_A6L7_ADH_rev_C02_08_ABI24.ab1
                1 GCGAGAATCT CAGAATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
              121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCT TTGTATAGTT
              181 CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC
              241 TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA
              301 AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC
              361 TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT
              421 CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT
              481 TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT
              541 CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT
              601 CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG
              661 GGTATCTTGA AAAGCATTGA ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA
              721 CAGGTAGTTT TCCAGTAGTG CAAATAAATT TAAAGGGTAA GTTTTCCGTA TGTTGCATCA
              781 CCTTCACCCT CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAAATTCAAC
              841 AAGAATTGGG ACACTCCAGT GAAAAGTTCT TTCTCCTTTA CCCATGGGTT TATCGTCATC
              901 ATCCCTTATA ATCCATATGA TGATGGTGGG TGATGCATAA GATCCAGGCC CTTGTCGACG
              961 CTAGCTTTAA ACAAAGCGAC TATAGTCAGA AAGTGAGATT GAGATGAAAT ATGACTTCCA
            1021 CTATCATTGG ATGGAATATG CAGGAATCCG TCTTCCTTAA AAATCGTCCA AGT
            //
            Primer: ADH-Forward
            Sequence file: RDB14461_A6L7c.seq check
            >06196_14461_A6L7_ADH-Forward_D02_11_ABI24.ab1
                1 AACCTCCTCT TAATTGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
               61 ACTAGTGCCT CTATTGATGC CTATGGAACC CCTTTGACTG ACGAGACTGT GAAGGCTTGT
              121 TTGGAAGCTG ACGGTGTTCT TTTGGGTGCC GTTGGTGGTC CTGAATGGAC CAACCCCAAT
              181 TGTCGTCCTG AGCAAGGTTT ATTGAAGCTT CGTAAGAGTA TGGGTGTTTG GGCCAACCTT
              241 CGACCTTGCA ACTTTGCCAG CAAGTCTTTA GTCAAGTACA GCCCTTTGAA GCCTGAAATC
              301 GTTGAAGGTG TCGATTTTTG TGTTGTACGA GAACTTACTG GAGGTTGTTA CTTTGGTGAG
              361 CGCACTGAGG ACAACGGATC GGGTTATGCT ATGGACACTT GGCCTTACAG TTTGGAAGAA
              421 GTTTCTCGTA TTGCTCGTTT GGCGGCCGCG GGCCCAACCT TCCAATTCAT TAAATCGAGT
              481 CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT AAGTAAGAGA AGTTGTTAGT
              541 GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG ATCTCTTCTC CATTGTTTCC
              601 TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT ATTTTCTGAT TATTTTTTTC
              661 TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG TAAAATCGGG GTATGGTTAG
              721 TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT AGGGGCAAGC GAGACAGAAT
              781 GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG TATTTTAATT TGTATTTTTT
              841 TGTAATTTTA TTTCTCATTG TTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC
              901 GAAATGTCAA AAATAAGCTC AGCAAAATAT ACAATTTTAC GGGAAAGCGA TCAGCAACTT
              961 TATTCTCCGT TTGTAGATTC TTTTTTCTTC ATAAATTCCC AACATGTAAA CTGCCCATTT
             1021 GATTTTTTCT CTAGATGCCT AATAAATTGA AACTTATGTA TAATAATTAC ACAAGGTTTT
            1081 GATAATGTAA ACTCTTACTC AGAAAAGGA
            //
            Primer: f1ori_R3
            Sequence file: RDB14461_A6L7d.seq check
            >06196_14461_A6L7_f1ori_R3_E02_14_ABI24.ab1
                1 GGGATGCTCT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
               61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
              121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
              181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG
              241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC
              301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG
              361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT
              421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT
              481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG
              541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG
              601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT
              661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC
              721 CAATTTTATA ATCAGGAAGT TTAAGTGAAT GGGTAGCACA TGTCGTAAAA ATTGTGAATT
              781 TTTATTGAAT AATATTTTTA AATACAAGCC CTTTTCTAGA CTAGGTATAC TCATAAACAT
              841 ATGAGCAAAA TGATAGAGGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT
              901 TACTGAAAAA TTAAATAATG AGTACTAAAT GATAAAAGCC GCCCTCAGTT ACAAGAAAGA
              961 TTGCAAAAAT ATTGCCATCG TCATGGACTC AAGGTGCCAT ATAGCCATAT TTGAAGATTG
            1021 ATAGTACTAA CTGCAGTTAG CGCG
            //
            Primer: Reverse2
            Sequence file: RDB14461_A6L7e.seq check
            >06196_14461_A6L7_Reverse2_F02_17_ABI24.ab1
                1 CGTACTCATT ACGCCAGCTT GTGATATTGA CGAAACTTTT TGACATCTAA TTTATTCTGT
               61 TCCAACACCA ATGTTTATAA CCAAGTTTTA TCTTGTTTGT CTACATGGTA TTTTACATTC
              121 ATCTACATAC ATCTTTCATT GGCTTTGTAC ATAGTTATCA TTACAAGTCT AAAAAAATTC
              181 ACTCTTTTCT TATTCAATGT CAATCCAAGA GAAAAGATTG TGGTAATGTT GTAGGAGCAT
              241 GTTTAATAAA TTACTATAGC AAATTACTTT TTATTCCCAA GGTGTTTATC TATAATAGTT
              301 AATATTTTAG TCGCTACATA AAATTTTACC AAAGAGTACT TGTATACTAA TTCTAAATGC
              361 CTTCTGACAT AAAACGCCTA GGAAAACAAA CGCAAACAAG GCATCGACTT TTTCAATAAC
              421 CAACCAAAAA AATTTTACAT TAGTCTTTTT TTAATGCTGA GAAAGTCTTT GCTGATATGC
              481 CTTCCAACCA GCTTCTCTAT ATCTCTTGGC TTCGACAACA GGATTACGAC CAGCTCCATA
              541 GACTCCACGA CCAACAATGA TGATGTCGCT ACCGCAGTTT ACAATCACTT CTTCAGGAGT
              601 ACGATATTGC TGTCCCAGCC CGTCTCCTTT AACATCCAAG CCGATACCAG GGGACATAGT
              661 TATGTAGTCG CTTTGAAAGT TAGGAAATCG ACGACCAGCT ATAAAGCCAA AGCAAAAATC
              721 GGTATGCTTC TCAAACCATT CTAAAGTTTT CTCTGTGTAG GAACCAGTAG CCAAAGAGCC
              781 TTTGGGAAGA CATTTCAGCC CAAAAGCAAG AGACCACGTC CCAAGGTAAA CCAAACTTCT
              841 TTTGAGGCCT TGTATATACC CCTCGCCTGG CACTGTATGG CAATTTGTGA TATGAGCCCA
              901 AGAAGCAATT TTGTACACAC CAGATGCATA TTGTAGCTGA CGTATTTCAT GTCTGCGATT
              961 GCGATCTTCA AAGAATAAGA AAACGAATGC TTTTACTTAA GCCACAGTTT TCTACGATAT
            1021 CTGCTCGTAT CTCGACACGG TCAATTGGTG CCTTGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            user_report Tanaka, T., Display of active beta-glucosidase on the surface of Schizosaccharomyces pombe cells using novel anchor proteins. Appl. Microbiol. Biotechnol., 97 (10): 4343-4352 (2013). PMID 23385477.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl