RDB06195 - pDUAL2-HFF81

Resource data sheet
Prev.

pDUAL2-HFF81

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (low strength) promoter

Catalog number	RDB06195
Resource name	pDUAL2-HFF81
Clone info.	S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (low strength) promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone	pDUAL2 (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364061.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06195	pDUAL2-HFF81	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06195z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14489_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw	RDB14489_A6L7a.seq
>06195_14489_A6L7_nmt1Fw_F01_16_ABI24.ab1 1 AACATTTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG 61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG 121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG 181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC 241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCTATGC 301 ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG GACTACAAGG 361 ACGACGATGA CAAAGATATC ATGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA 421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA 481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA 541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC 601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTGC CTCTATTGAT 661 GCCTATGGAA CCCCTTTGAC TGACGAGACT GTGAAGGCTT GTTTGGAAGC TGACGGTGTT 721 CTTTTGGGTG CCGTTGGTGG TCCTGAATGG ACCAACCCCA ATTGTCGTCC TGAGCAAAGG 781 TTTATTGAAG CTTCGTAAGA GTATGGGTGT TTGGGCCAAC CTTCGACCTT GCAACTTTGC 841 CAGCAAGTCT TTAGTCAAGT ACAGCCCTTT GAAGCCCTGA AATCGTTGAA GGTGTCGATT 901 TTTGTGTTGT ACGAGAACTT ACTGGAGGTT GTTACTTTGG TGGAGCGCAC TGAAGACACG 961 GGATCGGGTT ATGCTATGGG ACACTGGCCT TACAGTTTGG AGGAGGTTTT CTCGTATGCT 1021 CGATTGGGCG GCGCGGGCCG AAGCATTCAA ATCATCAAGT CGAAGTTCGT CAATCAGAAT 1081 GATTTTTACC AG //
Primer: ADH-Forward	RDB14489_A6L7b.seq
>06195_14489_A6L7_ADH-Forward_G01_19_ABI24.ab1 1 TGGTCACTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG 121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT 181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA 241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT 301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC 361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT 421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT 481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT 541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT 601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT 661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG 721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG 781 ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTTGT 841 AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT TAGAGAAAAC TTCAACGAAA 901 TGTCAAAATA AGCTCAGCAA AATATACAAT TTTTAGGGAA AGCGATCAGC AACTTTATTT 961 CTCCGTTTGT AGATTTCTTT TTCTTTCATA AATTTCCTAC AATGTAAACT GCCCAATTTG 1021 GATTTTTCTC TAGATGGCTA ATAAATCAAC CTTATTGAAT ATATACCAGT TTTGATAAAT 1081 TGTAAACTTC TTTACTTCGA //
Primer: f1ori_R3	RDB14489_A6L7c.seq
>06195_14489_A6L7_f1ori_R3_H01_22_ABI24.ab1 1 GGCATGGCTC AGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT 121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA 181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA 241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA 301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT 361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC 421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT 481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA 541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG 601 GCCCGAGTCT AACTCCTTAA CCACTCGGAC ATAGTGACTT ATCTGACACT CATTGTAAAT 661 TAATATATAT ATAGGCATTT TGTTTAGTTA AAGGTACTTA AGTAATTAGT ATAAACGAAC 721 CAATTTTATA ATCAGGAAGT TAAGTGAATG GTAGCACATG TCGTAAAAAT TGTGAATTTT 781 TATTGAATAA TATTTTAAAT ACAAGCCTTT CTAGACTAGG TATACTCATA AACATATGAG 841 CAAAAGGATA GAGGAGATTA CATTGCATCT TCTACAATTT ATTTATTGCC CCTTTACTGA 901 AAAAATTAAT AATGAGTACT AAATGAATAA AAAGCGCCTC AGTACAAGAA AAGATTTGCT 961 AAATATGCCA TTCCTTCCAT GCATCAAGTG GCATAATAAG CATATTGGCA GTATAGGTAC 1021 CTTAAACTGC AGTAGTCCGA AATTATTGA //
Primer: Reverse2	RDB14489_A6L7d.seq
>06195_14489_A6L7_Reverse2_A02_02_ABI24.ab1 1 CGGGAATCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG 61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT 121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT 181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA 241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT 301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG 361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA 421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG 481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT 541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG 601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG 661 TTATGTAGTC GCTTTGAAAG GTTAGGAAAT CGACGACCAG CTATAAAGCC AAAGCAAAAA 721 TCGGTATGCT TCTCAAACCA TTCTAAAGGT TTTCTCTGTG TAGGAACCCA GTAGCCAAGA 781 GCCTTTGGGA AGACATTTCA GCCCAAAAGC AGAGACCACG TCCCCAAAGG TAAACCAACT 841 TCTTTGAGCC TTGTATAATA CCCTCGCTGC ACTGTATGGC AAATTTGTGA ATATGAGCCA 901 GGAGCATTTG TACACACCAG ATGCATATGG TAGCCTGAAC GTATTTCGAA TGTCCTGCAA 961 ATAGCGAATC GTCAGGATAG AATCGATGCA TTAACTTAAA GCCACCA //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl