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pDUAL2-HFF81 (#RDB06195)

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (low strength) promoter

Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (RDB06192) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364061.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06195z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

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RDB06195 pDUAL2-HFF81 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-HFF81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06195).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14489_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14489_A6L7a.seq check
>06195_14489_A6L7_nmt1Fw_F01_16_ABI24.ab1
    1 AACATTTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG
  181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC
  241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCTATGC
  301 ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG GACTACAAGG
  361 ACGACGATGA CAAAGATATC ATGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA
  421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA
  481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA
  541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC
  601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTGC CTCTATTGAT
  661 GCCTATGGAA CCCCTTTGAC TGACGAGACT GTGAAGGCTT GTTTGGAAGC TGACGGTGTT
  721 CTTTTGGGTG CCGTTGGTGG TCCTGAATGG ACCAACCCCA ATTGTCGTCC TGAGCAAAGG
  781 TTTATTGAAG CTTCGTAAGA GTATGGGTGT TTGGGCCAAC CTTCGACCTT GCAACTTTGC
  841 CAGCAAGTCT TTAGTCAAGT ACAGCCCTTT GAAGCCCTGA AATCGTTGAA GGTGTCGATT
  901 TTTGTGTTGT ACGAGAACTT ACTGGAGGTT GTTACTTTGG TGGAGCGCAC TGAAGACACG
  961 GGATCGGGTT ATGCTATGGG ACACTGGCCT TACAGTTTGG AGGAGGTTTT CTCGTATGCT
 1021 CGATTGGGCG GCGCGGGCCG AAGCATTCAA ATCATCAAGT CGAAGTTCGT CAATCAGAAT
1081 GATTTTTACC AG
//
Primer: ADH-Forward
Sequence file: RDB14489_A6L7b.seq check
>06195_14489_A6L7_ADH-Forward_G01_19_ABI24.ab1
    1 TGGTCACTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
   61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
  121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
  181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
  241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
  301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
  361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
  421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
  481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
  541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
  601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT
  661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG
  721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG
  781 ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTTGT
  841 AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT TAGAGAAAAC TTCAACGAAA
  901 TGTCAAAATA AGCTCAGCAA AATATACAAT TTTTAGGGAA AGCGATCAGC AACTTTATTT
  961 CTCCGTTTGT AGATTTCTTT TTCTTTCATA AATTTCCTAC AATGTAAACT GCCCAATTTG
 1021 GATTTTTCTC TAGATGGCTA ATAAATCAAC CTTATTGAAT ATATACCAGT TTTGATAAAT
1081 TGTAAACTTC TTTACTTCGA
//
Primer: f1ori_R3
Sequence file: RDB14489_A6L7c.seq check
>06195_14489_A6L7_f1ori_R3_H01_22_ABI24.ab1
    1 GGCATGGCTC AGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
   61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
  121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
  181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
  241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
  301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
  361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
  421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
  481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
  541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
  601 GCCCGAGTCT AACTCCTTAA CCACTCGGAC ATAGTGACTT ATCTGACACT CATTGTAAAT
  661 TAATATATAT ATAGGCATTT TGTTTAGTTA AAGGTACTTA AGTAATTAGT ATAAACGAAC
  721 CAATTTTATA ATCAGGAAGT TAAGTGAATG GTAGCACATG TCGTAAAAAT TGTGAATTTT
  781 TATTGAATAA TATTTTAAAT ACAAGCCTTT CTAGACTAGG TATACTCATA AACATATGAG
  841 CAAAAGGATA GAGGAGATTA CATTGCATCT TCTACAATTT ATTTATTGCC CCTTTACTGA
  901 AAAAATTAAT AATGAGTACT AAATGAATAA AAAGCGCCTC AGTACAAGAA AAGATTTGCT
  961 AAATATGCCA TTCCTTCCAT GCATCAAGTG GCATAATAAG CATATTGGCA GTATAGGTAC
1021 CTTAAACTGC AGTAGTCCGA AATTATTGA
//
Primer: Reverse2
Sequence file: RDB14489_A6L7d.seq check
>06195_14489_A6L7_Reverse2_A02_02_ABI24.ab1
    1 CGGGAATCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAAG GTTAGGAAAT CGACGACCAG CTATAAAGCC AAAGCAAAAA
  721 TCGGTATGCT TCTCAAACCA TTCTAAAGGT TTTCTCTGTG TAGGAACCCA GTAGCCAAGA
  781 GCCTTTGGGA AGACATTTCA GCCCAAAAGC AGAGACCACG TCCCCAAAGG TAAACCAACT
  841 TCTTTGAGCC TTGTATAATA CCCTCGCTGC ACTGTATGGC AAATTTGTGA ATATGAGCCA
  901 GGAGCATTTG TACACACCAG ATGCATATGG TAGCCTGAAC GTATTTCGAA TGTCCTGCAA
961 ATAGCGAATC GTCAGGATAG AATCGATGCA TTAACTTAAA GCCACCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]