RDB06194 - pDUAL2-HFF41

Resource data sheet
Prev.

pDUAL2-HFF41

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter

Catalog number	RDB06194
Resource name	pDUAL2-HFF41
Clone info.	S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter. Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone	pDUAL2 (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364060.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06194	pDUAL2-HFF41	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06194z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14488_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw	RDB14488_A6L7a.seq
>06194_14488_A6L7_nmt1Fw_D04_04_ABI08.ab1 1 TGAAATTTAA TTTAATCAAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA 61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT 121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA 181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC 241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC 301 CTATGCATCA CCACCATCAT CATATGGATT ATAAGGATGA TGACGATAAA CCCATGGACT 361 ACAAGGACGA CGATGACAAA GATATCATGA GATCTCGACT CTAGAGGATC CGGCGCGCCA 421 CTTCTAAATA AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA GTTATAAAAA 481 AAATAAGTGT ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA TTCTTATTCT 541 TGAGTAACTC TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG GTCGCTCTTA 601 TTGACCACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC TAGTGCCTCT 661 ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT GGAAGCTGAC 721 GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG TCGTCCTGAG 781 CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG ACCTTGCAAC 841 TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT TGAAGTGTCG 901 ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGGTGAGCC GCCACTGAGG 961 ACACGATCGG TTATGCTATG GACCACTTGG CCCTTACAGT TGGAAGAAGT TTCCTCGTAA 1021 TGGCCTCGAT TTGGCGGCCC GCGGGCCGAC TCCCAATCCA ATTGATCGAG TCGTGATCGA 1081 ATGAATTACA ACGTTTTTGC GACTA //
Primer: ADH-Forward	RDB14488_A6L7b.seq
>06194_14488_A6L7_ADH-Forward_E04_05_ABI08.ab1 1 ATCTCCTTTT AATTGAAACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA 61 CTAGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT 121 TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT 181 GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC 241 GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG 301 TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC 361 GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG 421 TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACCTT CCAATTCATT AAATCGAGTC 481 CTCCATCAGA TGATTTACAA GGTTTTGCAT AGAATCCCTA AGTAAGAGAA GTTGTTAGTG 541 ATTTCTTCAA GTTTACTAAA ATTTACATAC AAATGGATGA TCTCTTCTCC ATTGTTTCCT 601 TTCTTCTTGC AAACGCGTTT TACAAAGAGA TGACATGCTA TTTTCTGATT ATTTTTTTCT 661 ATAGTTTTCT ATTTTGTACG TCCTTGGGAG TCCGAAATGT AAAATCGGGG TATGGTTAGT 721 AGCGGCAGCG TTCATTATGG TATATAAAAG ATAAAAAAGT AGGGGCAAGC GAGACAGAAT 781 GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAAATT GTATTTTAAT TTGTATTTTT 841 TGTAAATTTT ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTTAAAG AAAAACTTCA 901 ACGAAATGGT CAAAATTAAA GCTCCACAAA AATATTACCA ATTTTTAGGG AAAGCCGATT 961 CAGCAACTTT ATTCTTCCGG TTTGTAGGAT TTCTTTTTTC TTTCAATAAA TTCCAACCAT 1021 GCTACCTGGC CAATTGAATT CTTTCTCTCT AAATTGCTAT TAATTGAAAC CTAATGGATA 1081 TACTACCACA GCGTTTTTTG GACATTAAAT TGTTATGAAC TAT //
Primer: f1ori_R3	RDB14488_A6L7c.seq
>06194_14488_A6L7_f1ori_R3_F04_06_ABI08.ab1 1 GGCGTAGGGT CCTTGGCAGT ATGCGGGTGT GAATACCGCA CAGATGCGTA AGGAGAAAAT 61 ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC 121 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT 181 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC 241 TCGGGAGCGC TACCGTGAAT GGGCTCAACC AAACAATGAA CCTTTTCTTC TGATTTTCCT 301 ACCACACCGG AAAGGGAGGC AGAAGGCAAA AGGCCCAAGC TACCAGGAAT GACAGAAGCC 361 TCATCTGAAA TAATGTCACC AAACAAGTTG TCAGTCAAAA CAACACCGTT AAGTGTACGA 421 GGGCTCTTGA CCAAAAGCAT GGCTGCGGAG TCAATGAGCT GGTTTTTTAA GGTAAGGTGA 481 GGATATTCCT CCTTAAAAAT CTTAGCTACA GTCTTGCGCC AAAGACGAGA AGTTGCCAAA 541 ACATTAGCTT TGTCGAGTAA TGTGACGGGA GCAGGAGGGT TGGAAGTTTC AGCTAAGCGG 601 CCGCGGGCCC GAGTCTAACT CCTTAACCAC TCGGACATAG TGACTTATCT GACACTCATT 661 GTAAATTAAT ATATATATAG GCATTTTGTT TAGTTAAAGG TACTTAAGTA ATTAGTATAA 721 ACGAACCAAT TTTATAATCA GGAAGTTAAG TGAATGGTAG CACATGTCGT AAAAATTGTG 781 AATTTTTATT GAATAATATT TTAAATACAA GCCTTTCTAG ACTAGGTATA CTCATAAACA 841 TATGAGCAAA AGGATAGAGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT 901 TACTGAAAAA ATTAAATAAT GAGTACTAAA TGATAAAAAG CGCTCAGTAC AAGAAAGATT 961 GCAAAAATAT TGCATTCTTC ATGAATTTAA AGGTTTGCAT AATAAGGGCA TATTTGAAAG 1021 TAATAGTACT AAAACAGCCA GGTTAAGCCG AGATATTAGA GTTATTATTC GCAGACATGT 1081 GACCATTAGA TTAAAAAGAC TTTGTAAGAC //
Primer: Reverse2	RDB14488_A6L7d.seq
>06194_14488_A6L7_Reverse2_H09_24_ABI24.ab1 1 CATAAATCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG 61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT 121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT 181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA 241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT 301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG 361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA 421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG 481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT 541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG 601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG 661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT 721 CGGTATGCTT TCTCAAACCA TTCTAAGGTT TTCTCTGTGT AGGAAACCAG TAGCCCAAAG 781 AGCCTTTTGG AAGACATTTC AACCCAAAAG CAAAGAGACC ACGTCCCAAA GGTAAACCAA 841 CTTTCTTTGA GGCCCTTTGT ATAATACCCC TCGCCCTGGC ACTGTATGGC AATTTTGTGA 901 TATGAGCCCC AAGAAGCAAT TTTGTACACA CCAGATGCCT ATTTGGTAAC TTTGACCGGT 961 ATTTTCCAAT TGTTCGGCGA AAT //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl