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pDUAL-YFH1

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06191
Resource name pDUAL-YFH1
Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06191 pDUAL-YFH1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06191z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13523_A5Ipp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB13523_A5Ipa.seq
>06191_13523_A5Ip_nmt1Fw_D05_11.ab1
    1 GAGAATAATT TATTAAATCA GGGAAAAACG TAACTCTCGG CTACTGGATG GTTCAGTCAC
   61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC
  121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA
  181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT
  241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC
  301 CTGGATCCGG ATATCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
  361 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG
  421 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
  481 GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCC TGCAGTGCTT CGCCCGCTAC
  541 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
  601 GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC
  661 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC
  721 AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC
  781 GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC
  841 AGCGTGCAGC TCGCCGACCA CTACCAGCAA GAACACCCCC ATCGGCGACG GCCCCGTGCT
  901 GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
  961 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGG
 1021 ACGAGCTGTA CAGCCCATGG ACTACAAGGA CGAACGATGA CAAACATCAC CACCATCACC
 1081 ACTGAGATCT CGACTCTAGA GATCCGGGGC GCCACTTCTA AAATAAGCGG AATTCTATGA
 1141 ATTTATGAAT TTTAATAATT AAATAGTTAT TAAAAAAATA TAGGGTTGTA TATTCTCAAC
1201 A
//
Primer: ADH_rev RDB13523_A5Ipb.seq
>06191_13523_A5Ip_ADHrev_E05_14.ab1
    1 TGAAAGTTAT CAGATAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
   61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
  121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA GTGGTGATGG TGGTGATGTT
  181 TGTCATCGTC GTCCTTGTAG TCCATGGGCT TGTACAGCTC GTCCATGCCG AGAGTGATCC
  241 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC
  301 TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA
  361 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC
  421 GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT
  481 GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA
  541 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG
  601 TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA
  661 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGGCG AAGCACTGCA
  721 GGCCGTAGCC GAAGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC
  781 AGATGAACTT CAGGGTCAGC TTGCCGTAGT GGCATCGCCC TCGCCCTCGC CGGACACGCT
  841 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA
  901 CAGCTCCTCG CCCTTTGCTC ACCATGATAT CCGGGATCCA GGCCTGTCGA CGCTAGCTTT
  961 AACAAAGCGA ACTAATAGTC AGAAAGTGAG AATTGAGATG GAAATCTACT TCACTATCAA
1021 TTGATGGATA TGGCAGGATT CGTCATCTAT GAATATAATC TGCAAATGT
//
Primer: ADH-Forward RDB13523_A5Ipc.seq
>06191_13523_A5Ip_ADH-Forward_F05_17.ab1
    1 TCCCCCCTTT TAATTGGACA CACCTTCTAC CGGGCAGATC CGCTAGGGAT AACAGGGTAA
   61 TATACTAGTA AGATGATTCT TTTATCGTCG ATAACGATGA GGATGTAGAC GATTATGATG
  121 AGAGTGATTA GCTTCTAAAA TTTGGTGTTT TCAAACGCTA TTTACAGTAC AATCTGAAAA
  181 AATTGGATGA ACATTGTAAA TGGTAGGATA CGAGTTCCAA TTAATGTATT TTTTAAGTAT
  241 TACACTTAGC TTATTAATTT TATATATACA TTCTACCTCT GTGGGTTTAA AGACAACTCG
  301 TACACATACT CAAATTATTT ATGAACATTA TAATGCATAT TCGTACTGTC TATTAAACAT
  361 TTTTTTGACA TTAAAAATTT CGTTTACTAA CGTAGAAAGC GCTGATAATT GAGGCTACTA
  421 TTTTAATATC TAGAAACAAA GATTCATTAA TTAATTAAGT AAGCTCCAGG ATACTTGTAT
  481 ATTTCGTTAA AAAAGCGCAA TTTCAACAAT TCCTATGAAC ATCCATGATT TCATCACAAA
  541 ATCAAACATA TCAGGATTTA ACGTGAATTA TTTGCTTTCT TGTGATTTTC ATCTGATTTC
  601 TGGTCATTTA CGTTACTGTA AACAAGCTTT TTAAAAAAAG GATTTTTTAC GCAATATTAT
  661 CGATATCCCA ATCTGTAGTA TAAAAAGTGC AACAGTGTTG AGTTTCCCGA AACCAGGCAT
  721 TTTTGCGGTA GGGTCTGAAA CTTTCACGAA ACCATTTCAC AACAAAACTT CTTCAACAAC
  781 ATGTGTGCAA AGAAAAATCG TCGTCTTACC AGGAGACCAT ATTGGCCCTG AAATTGTTGC
  841 TTCTGCCTTG GAGGTTTTGA AAGTCGTTGA GAAGAAGCGA CCTGAGTTAA AACTCGAGTT
  901 TGAAGAACAC AAGATTGGAG GTGCCTCTAT TGATGCCCTA TGGAAACCCC TTTGACTGAC
  961 GAGACTGTGA AGGCTTGTTT GGAAGCTGAC GGTGTTCTTT TGGTGCCGTT GGGTGGTCCT
 1021 GAATGGACCA ACCCCAATTG TCGTCCTGAG CCAAGGTTTA TTGAAGCCTT CGTTAGAGTA
 1081 TGGGTTGTTT GGGGCAACCT TTCGACCTGC AACTTTGCCA GCAAGTCTTA GGTCAGTAAC
 1141 AGCCCTTTGC AGCCCTGGAA TCGGTTGGAA CGGTGTCGCA TTTTTGCGTT GGAGCAGAGA
1201 AAACCATATA CTG
//
Primer: ars_R1 RDB13523_A5Ipd.seq
>06191_13523_A5Ip_ars_R1_G05_20.ab1
    1 TGGTTCCGTT TTAAAACTAA TTTACTTAAA GTACCCTTTA ACTAAACAAA AATGCCTATA
   61 TATATAATTA ATTTACAATG AGTGTCAGAT AAGTCACTAT GTCCGAGTGG TTAAGGAGTT
  121 AGACTCGAAT TCGCAAGCTT AGCTACAAAT CCCACTGGCT ATATGTATGC ATTTGTGTTA
  181 AAAAAGTTTG TATAGATTAT TTAATCTACT CAGCATTCTT TCTCTAAATA GGAATTTGTT
  241 ACTTAATGGA GAAAAAAATG TTTCGATTTA CCTAGTGTAT TTGTTTGTAT ACTCACGTTT
  301 AATTTCAAAC ATCCATTCTA TCTTGTGTAA TTTTTGGCAT GGTGAAAAAG ATAATCAGCC
  361 TTATAATCTT TACAAAAGTA AGAAATTCTG TAAATAAGCC TTAATGCCCT TGCTTTAAAT
  421 TAAAATGGTT CTTTTTCATG ATAATGTTTG CACTTTGTGA ATATATTTTA GATAGTTCTG
  481 TGAGGTATAA TTAAGATGTT TTAGAGACTT ATACAATTTT GTCTTTATAA ATTCTTAATT
  541 GATTTTACCA TCCCAGTTTA ACTATGCTTC GTCGGCATCT CTGCACATGT CGTGTTTTCT
  601 TACCGTATTG TCCTACCAAG AACCTCTTTT TTGCTTGGAT CGAAATTAAA GGTTTAAAAG
  661 CAAAGTTATG GATGCTAGAG TATTTCAAAG CTATTCAGCT AGAGCTGAGG GGATGAAAAA
  721 TCCCATTGCC AAGGAATTGT TGGCTTTGAT GGAAGAAAAG CAAAGCAACT TGTCAGTCGC
  781 GGTCGATTTG ACGAAGAAAA TCCGAAATCT TAGAAATTGG TAGAATAAAA ATTGGACCCT
  841 ATGTCTGTGT TATCAAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCC ACGATATTGG
  901 TAGAAAAAAC TGGGTGGCTC TTAGGTAAAA GCATTCGTTT TCTTATCTTT GAAGGATCGC
  961 AAAATTCGCA GAACATTGGA AAATACCGTC AAGCTACAAT ATGCATTCTG GTGTGAACAA
 1021 AATTGCTTTC TTGGGGCTCA AATCACAAAT TGCCATAAGT GCCCAAGGCG AGGGTATTAA
 1081 TCCAAGGGCC TCAAAAAAAT TGGTTAACCT TTGGGAACGT GGTCCCCTTG CTTTTTCGCC
1141 TGAAAAGTGT CTTCCAAAGG GCTCTTTTGG CTTAC
//
Primer: f1ori_R3 RDB13523_A5Ipe.seq
>06191_13523_A5Ip_f1ori_R3_H05_23.ab1
    1 GCGGGGGGCT GAAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
   61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
  121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
  181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
  241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
  301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
  361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
  421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
  481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
  541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
  601 GCCCAACCTT CCAATTCATT AAATCGAGTC CTCCATCAGA TGATTTACAA GGTTTTGCAT
  661 AGAATCCCTA AGTAAGAGAA GTTGTTAGTG ATTTCTTCAA GTTTACTAAA ATTTACATAC
  721 AAATGGATGA TCTCTTCTCC ATTGTTTCCT TTCTTCTTGC AAACGCGTTT TACAAAGAGA
  781 TGACATGCTA TTTTCTGATT ATTTTTTTCT ATAGTTTTCT ATTTTGTACG TCCTTGGGAG
  841 TCCGAAATGT AAAATCGGGG GTATGGTTAG TAGCGGCAGC GTTTCATTAT GGTATATAAA
  901 AGATAAAAAA GTATGGGCAA GCGAGACAGA AATGGGGATA CAAAGGCATC GTCTATAATT
  961 ATAGCTAAAA ATTGTATTTT TAAATTTGTA TTTTTTTTGT AAATTTTATT TTCTCATTGT
 1021 TTTTACTTTA AAATGAATGC GAATTTAGAG AAAAACTTCC ACGAAAATGG TCGAAAATAA
1081 GCTCAAGCAA AAGTATACGA ATTTTTAAGG GC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
reference Wei, Y., SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage. Mol. Cell 66 (5): 581-596 (2017). PMID 28552615.
user report Dujeancourt, L., Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. Mitochondrion 13 (6): 871-880 (2013). PMID 23892058.
user report Kuhl, I., A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression. Nucleic Acids Res., 39 (18): 8029-8041 (2011). PMID 21727087.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl