RDB06191 - pDUAL-YFH1

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pDUAL-YFH1 (#RDB06191)

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Clone info.	S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter.
Comment	Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone	pDUAL (RDB06178) (Plasmid)
Selectable markers	Amp^r
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06191z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06191	pDUAL-YFH1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-YFH1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06191).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13523_A5Ipp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F (Pr0536) Region: insert 5'(nmt1 pro 5') Sequence file: RDB13523_A5Ipf.seq
>06191_13523_B2DsS_pUC_CAPbs_up_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTAGCTCAC TCNATTAGGC 61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTTGCAT GCCTGCAGGT 181 CGATCGACTC TAGAGGATCA GAAAATTATC GCCATAAAAG ACAGAATAAG TCATCAGCGG 241 TTGTTTCATT TCCTATATTT TTTTTTTATT TTTTTATTTT TTAATAAGGG AAAATTTAAC 301 GTCTAAGGAT ACAGAAGATT GTTAGCACAT TAAAGTAATA AAGGCTTAAG TAGTAAGTGC 361 CTTAGCATGT TATTGTATTT CAAAGGACAT AATCTAAAAT AATAACAATA TCATTTCTCA 421 CAAGTTATTC AATTTTCTTT TTTTTTTCTA ATAATATCAA GAATGTATTA TTTGTTTGAC 481 ATAAGTCAAC TAATTTATTT AATATGCTGG ATTAATCTTG CAGACATGTA AATTAACAAG 541 TTTTAGTCAA ATAACGTTGA AGTTTCNATG AACTCNAATA ATTTCTCTTT TTTTTTATAT 601 AACNNTAATC TGATTTATAT TTTCCGCANG GATNNNNTGN AGTTATGACA TTTGGNNTNN 661 NTCACTTANN NNCNNGGTCG CCAAATNATA CNAAATCANC GNTANAAAAC NNNNNNNTTT 721 NGNNAANNAA NNNNNNNNNN //
Primer: ADHrev (Pr0048) Region: ADH ter,insert 3'(6xHis,FLAG,YFP 3') Sequence file: RDB13523_A5Ipg.seq
>06191_13523_A5Ip_ADHrev_E05_14.ab1 1 NNNNNNNNNN NNNNNNNANT TTCGTTTTNN ACCTAAGAGT CACTTTAAAA TTTGTATACA 61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT 121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA GTGGTGATGG TGGTGATGTT 181 TGTCATCGTC GTCCTTGTAG TCCATGGGCT TGTACAGCTC GTCCATGCCG AGAGTGATCC 241 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC 301 TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA 361 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC 421 GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT 481 GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA 541 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG 601 TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA 661 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGGCG AANCACTGCA 721 GGCCGTANCC GAANGTGGTC ACGANGGTGG GCCAGGGCAC GGGCAGCTTG CCGGNGGTGC 781 AGATGAACTT CANGGTCAGC TNGCCGNNNN GGCNTCGCCC TCGCCCNCGC CGGNNNNGCN 841 NAACTTGNGG NCNNTTACGT NNNNNNCCNN CTCNNNCANN NNGGGNANNN NCNNNNNNNN 901 NN //
Primer: nmt1Fw (Pr0004) Region: insert mid(nmt1 pro 3',YFP 5') Sequence file: RDB13523_A5Iph.seq
>06191_13523_A5Ip_nmt1Fw_D05_11.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTAACTC TCGGCTACTG GNNGGTTCNG TCACCCAACG 61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG 121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT 181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT 241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT 301 CCGGATATCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC 361 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT 421 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC 481 TGGCCCACCC TCGTGACCAC CTTCGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC 541 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AANGCTACGT CCAGGANCGC 601 ACCATCTTCT TCAAGGACGA CGGCAACTAC NAGACCCGCG CCGANGTGAA GTTCGAGGGC 661 GACACCCTGG TGAACCGCAT CGAGCTGAAN GGCATCGACT TCAAGGANGA NGGCAACNTC 721 CTGGGGCACN AGCTGNANTA NNNCTACNNC AGCCNCNACG TCTATATCAT GGNNGANAAN 781 CAGAANNACG GNNTCNNNNN GNNNTTNNNN NNNGNNNNNA NATCNANNAN NNNNNNNNGC 841 NNNNNNNNNA NNNNTNNNNN NNNNN //
Primer: URA4pro_F (Pr0371) Region: ura4+ mid Sequence file: RDB13523_A5Ipi.seq
>06191_13523_B2DsS_URA4pro_F_C01_03_ABI08.ab1 1 NNNNNNNNNN CCNNNNNNNN ACCTCTTTTT TGCTTGGATC GAAATTAAAG GTTTAAAAGC 61 AAAGTTATGG ATGCTAGAGT ATTTCAAAGC TATTCAGCTA GAGCTGAGGG GATGAAAAAT 121 CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT GTCAGTCGCG 181 GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGTAG ATAAAATTGG ACCCTATGTC 241 TGTGTTATCA AGACACATAT TGACGTTGTC GAGGATTTCG ACCAGGATAT GGTAGAAAAA 301 CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA ATTCGCAGAC 361 ATTGGAAATA CCGTCAAGCT ACAATATGCA TCTGGTGTGT ACAAAATTGC TTCTTGGGCT 421 CATATCACAA ATTGCCATAC AGTGCCAGGC GAGGGTATTA TACAAGGCCT CAAAGAAGTT 481 GGTTTACCTT TGGGACGTGG TCTCTTGCTT TTGGCTGAAA TGTCTTCCAA AGGCTCTTTG 541 GCTACTGGTT CCTACACAGA GAAAACCTTA GAATGGTTTG AGAAGCATAC CGATTTTTGC 601 TTTGGCTTTA TAGCTGGTCG TCGATTTCCT AACCTTCNAA NCGACTACNT AACTATGTCC 661 CCNGNNATCN GCTTNNNNGN NAANNNNANG GNCTGGGNNN GCNANATCNN ACTNNNNNAN 721 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
reference	Wei, Y., SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage. Mol. Cell 66 (5): 581-596 (2017). PMID 28552615. [link to RRC of NBRP]
user_report	Dujeancourt, L., Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. Mitochondrion 13 (6): 871-880 (2013). PMID 23892058. [link to RRC of NBRP]
user_report	Kuhl, I., A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression. Nucleic Acids Res., 39 (18): 8029-8041 (2011). PMID 21727087. [link to RRC of NBRP]

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