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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-YFH1

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06191
Resource name pDUAL-YFH1
Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06191 pDUAL-YFH1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06191z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13523_A5Ipp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pUC-CAPbs_up_F
            Region: insert 5'(nmt1 pro 5')
            Sequence file: RDB13523_A5Ipf.seq check
            >06191_13523_B2DsS_pUC_CAPbs_up_F_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTAGCTCAC TCNATTAGGC
               61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
              121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTTGCAT GCCTGCAGGT
              181 CGATCGACTC TAGAGGATCA GAAAATTATC GCCATAAAAG ACAGAATAAG TCATCAGCGG
              241 TTGTTTCATT TCCTATATTT TTTTTTTATT TTTTTATTTT TTAATAAGGG AAAATTTAAC
              301 GTCTAAGGAT ACAGAAGATT GTTAGCACAT TAAAGTAATA AAGGCTTAAG TAGTAAGTGC
              361 CTTAGCATGT TATTGTATTT CAAAGGACAT AATCTAAAAT AATAACAATA TCATTTCTCA
              421 CAAGTTATTC AATTTTCTTT TTTTTTTCTA ATAATATCAA GAATGTATTA TTTGTTTGAC
              481 ATAAGTCAAC TAATTTATTT AATATGCTGG ATTAATCTTG CAGACATGTA AATTAACAAG
              541 TTTTAGTCAA ATAACGTTGA AGTTTCNATG AACTCNAATA ATTTCTCTTT TTTTTTATAT
              601 AACNNTAATC TGATTTATAT TTTCCGCANG GATNNNNTGN AGTTATGACA TTTGGNNTNN
              661 NTCACTTANN NNCNNGGTCG CCAAATNATA CNAAATCANC GNTANAAAAC NNNNNNNTTT
            721 NGNNAANNAA NNNNNNNNNN
            //
            Primer: ADHrev
            Region: ADH ter,insert 3'(6xHis,FLAG,YFP 3')
            Sequence file: RDB13523_A5Ipg.seq check
            >06191_13523_A5Ip_ADHrev_E05_14.ab1
                1 NNNNNNNNNN NNNNNNNANT TTCGTTTTNN ACCTAAGAGT CACTTTAAAA TTTGTATACA
               61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
              121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA GTGGTGATGG TGGTGATGTT
              181 TGTCATCGTC GTCCTTGTAG TCCATGGGCT TGTACAGCTC GTCCATGCCG AGAGTGATCC
              241 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC
              301 TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA
              361 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC
              421 GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT
              481 GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA
              541 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG
              601 TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA
              661 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGGCG AANCACTGCA
              721 GGCCGTANCC GAANGTGGTC ACGANGGTGG GCCAGGGCAC GGGCAGCTTG CCGGNGGTGC
              781 AGATGAACTT CANGGTCAGC TNGCCGNNNN GGCNTCGCCC TCGCCCNCGC CGGNNNNGCN
              841 NAACTTGNGG NCNNTTACGT NNNNNNCCNN CTCNNNCANN NNGGGNANNN NCNNNNNNNN
            901 NN
            //
            Primer: nmt1Fw
            Region: insert mid(nmt1 pro 3',YFP 5')
            Sequence file: RDB13523_A5Iph.seq check
            >06191_13523_A5Ip_nmt1Fw_D05_11.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNTAACTC TCGGCTACTG GNNGGTTCNG TCACCCAACG
               61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
              121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
              181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
              241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
              301 CCGGATATCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC
              361 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT
              421 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC
              481 TGGCCCACCC TCGTGACCAC CTTCGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC
              541 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AANGCTACGT CCAGGANCGC
              601 ACCATCTTCT TCAAGGACGA CGGCAACTAC NAGACCCGCG CCGANGTGAA GTTCGAGGGC
              661 GACACCCTGG TGAACCGCAT CGAGCTGAAN GGCATCGACT TCAAGGANGA NGGCAACNTC
              721 CTGGGGCACN AGCTGNANTA NNNCTACNNC AGCCNCNACG TCTATATCAT GGNNGANAAN
              781 CAGAANNACG GNNTCNNNNN GNNNTTNNNN NNNGNNNNNA NATCNANNAN NNNNNNNNGC
            841 NNNNNNNNNA NNNNTNNNNN NNNNN
            //
            Primer: URA4pro_F
            Region: ura4+ mid
            Sequence file: RDB13523_A5Ipi.seq check
            >06191_13523_B2DsS_URA4pro_F_C01_03_ABI08.ab1
                1 NNNNNNNNNN CCNNNNNNNN ACCTCTTTTT TGCTTGGATC GAAATTAAAG GTTTAAAAGC
               61 AAAGTTATGG ATGCTAGAGT ATTTCAAAGC TATTCAGCTA GAGCTGAGGG GATGAAAAAT
              121 CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT GTCAGTCGCG
              181 GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGTAG ATAAAATTGG ACCCTATGTC
              241 TGTGTTATCA AGACACATAT TGACGTTGTC GAGGATTTCG ACCAGGATAT GGTAGAAAAA
              301 CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA ATTCGCAGAC
              361 ATTGGAAATA CCGTCAAGCT ACAATATGCA TCTGGTGTGT ACAAAATTGC TTCTTGGGCT
              421 CATATCACAA ATTGCCATAC AGTGCCAGGC GAGGGTATTA TACAAGGCCT CAAAGAAGTT
              481 GGTTTACCTT TGGGACGTGG TCTCTTGCTT TTGGCTGAAA TGTCTTCCAA AGGCTCTTTG
              541 GCTACTGGTT CCTACACAGA GAAAACCTTA GAATGGTTTG AGAAGCATAC CGATTTTTGC
              601 TTTGGCTTTA TAGCTGGTCG TCGATTTCCT AACCTTCNAA NCGACTACNT AACTATGTCC
              661 CCNGNNATCN GCTTNNNNGN NAANNNNANG GNCTGGGNNN GCNANATCNN ACTNNNNNAN
            721 NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            reference Wei, Y., SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage. Mol. Cell 66 (5): 581-596 (2017). PMID 28552615.
            user_report Dujeancourt, L., Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. Mitochondrion 13 (6): 871-880 (2013). PMID 23892058.
            user_report Kuhl, I., A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression. Nucleic Acids Res., 39 (18): 8029-8041 (2011). PMID 21727087.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl