Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-GFH81

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14460_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14460_A6L7a.seq
>06190_14460_A6L7_nmt1Fw_A01_01_ABI24.ab1     1 TACATTTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT    61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA   121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATGGAA   181 GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA TCTCATTCTC   241 ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA TCCGGATATC   301 ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT   361 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA   421 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT   481 GTCACTACTT TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG   541 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC   601 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT   661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA   721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA   781 ATCAAAGTTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGGAAGCGTT TCAACTAGCA   841 GACCATTTAT CAACAAAAAT ACTCCAATTT GGCGATGGGC CCTGTCCTTT TACCAGACAC   901 CATTTACCCT GTCCACAACA ATCTGCCCCT TTCGAAAGAT CCCAAACGGA AAAGAGAGAC   961 ACATGGTCCT TCTTGGAGTT GTACAGCTGC TTGGATACAC ATGCATGATG ACCTATACAA 1021 TCCATGGACT TACAGGAACG ACGATGACTA CCATACTAGC GATCACTACT GGAGATA //
Primer: ADH_rev Sequence file: RDB14460_A6L7b.seq
>06190_14460_A6L7_ADH_rev_B01_04_ABI24.ab1     1 GGGAGAGTTC TCAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA    61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT   121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG   181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG TTTGTATAGT TCATCCATGC CATGTGTAAT   241 CCCAGCAGCT GTTACAAACT CAAGAAGGAC CATGTGGTCT CTCTTTTCGT TGGGATCTTT   301 CGAAAGGGCA GATTGTGTGG ACAGGTAATG GTTGTCTGGT AAAAGGACAG GGCCATCGCC   361 AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG CTTCCATCTT CAATGTTGTG   421 TCTAATTTTG AAGTTAACTT TGATTCCATT CTTTTGTTTG TCTGCCATGA TGTATACATT   481 GTGTGAGTTA TAGTTGTATT CCAATTTGTG TCCAAGAATG TTTCCATCTT CTTTAAAATC   541 AATACCTTTT AACTCGATTC TATTAACAAG GGTATCACCT TCAAACTTGA CTTCAGCACG   601 TGTCTTGTAG TTCCCGTCAT CTTTGAAAAA TATAGTTCTT TCCTGTACAT AGCCTTCGGG   661 CATGGCACTC TTGAAAAAGT CATGCTGTTT CATGTGATCT GGGTATCTTG AAAAGCATTG   721 AACACCATAA CAGAAAGTAG TGACAAGTGT TGGCCACGGA AACAGGTAGT TTTCCAGTAG   781 TGCAAATAAA TTTAAAGGGT AAGTTTTCCG TATGTTGCAT CACCTTCACC CTCTCCACTG   841 ACAGAAAATT TGTGCCATTA ACATCACCCA TCTAATTTCA ACAAGAATTG GGGACACTCC   901 CAGTTGAAAG TTCTCTCCTT TTACTCATGA TATCCCGGAT CCAGGGCCTG TCGACGCTTA   961 GGCTTTACAA AGCGAACTAT TAGGTCAGAA GGTGGAGAAT TGGAGATTGG AATTAATATC 1021 AACTTATCAT TGATGAATAT TGGCCAAGGC ATC //
Primer: ADH-Forward Sequence file: RDB14460_A6L7c.seq
>06190_14460_A6L7_ADH-Forward_C01_07_ABI24.ab1     1 TGGTCATTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT    61 AGTAAGATGA TTCTTTTATC GTCGATAACG ATGAGGATGT AGACGATTAT GATGAGAGTG   121 ATTAGCTTCT AAAATTTGGT GTTTTCAAAC GCTATTTACA GTACAATCTG AAAAAATTGG   181 ATGAACATTG TAAATGGTAG GATACGAGTT CCAATTAATG TATTTTTTAA GTATTACACT   241 TAGCTTATTA ATTTTATATA TACATTCTAC CTCTGTGGGT TTAAAGACAA CTCGTACACA   301 TACTCAAATT ATTTATGAAC ATTATAATGC ATATTCGTAC TGTCTATTAA ACATTTTTTT   361 GACATTAAAA ATTTCGTTTA CTAACGTAGA AAGCGCTGAT AATTGAGGCT ACTATTTTAA   421 TATCTAGAAA CAAAGATTCA TTAATTAATT AAGTAAGCTC CAGGATACTT GTATATTTCG   481 TTAAAAAAGC GCAATTTCAA CAATTCCTAT GAACATCCAT GATTTCATCA CAAAATCAAA   541 CATATCAGGA TTTAACGTGA ATTATTTGCT TTCTTGTGAT TTTCATCTGA TTTCTGGTCA   601 TTTACGTTAC TGTAAACAAG CTTTTTAAAA AAAGGATTTT TTACGCAATA TTATCGATAT   661 CCCAATCTGT AGTATAAAAA GTGCAACAGT GTTGAGTTTC CCGAAACCAG GCATTTTTGC   721 GGTAGGGTCT GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT   781 GCAAAGAAAA TCGTCGTCTT ACCAGGAGAC CATATTGGGC CCTGAAATTG TTGCTTCTGC   841 CTTGGAGGTT TTGAAAGTCG TTGAGAAGAA GCGACCTGAG TTAAAACTCG AGTTTGAAGA   901 ACACAAGATT GGAGTGCCTC TATTGATGCC TATGGGAACC CCTTTGACTG ACGAGACTGT   961 GAAGGCTTGT TGAAGCTGAC GGTGTTCCTT TTGGGTGCGG TGTGTCTGAA TGACACCCAT  1021 GTCGTCTGAG CCAGGTTAAT GAGCCTCGTT ACAGTATGCG TGTTTCGGCC TGATCCATCC 1081 GACTTTGCCG ACT //
Primer: ars_R1 Sequence file: RDB14460_A6L7d.seq
>06190_14460_A6L7_ars_R1_D01_10_ABI24.ab1     1 TGGTTCGTTT ATACTAATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT    61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA   121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT   181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG   241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA   301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC   361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG   421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT   481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC   541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT   601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA   661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG   721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT   781 TGACGAAGAA ATCCGAAATC TTAGAATTTG GTAGATAAAA TTGGACCCTA TGTCTGTGTT   841 ATCAAGACAC ATATTGACGT TGTCGAGGAT TTCGACCAGG ATATGGGTAG AAAAAACTGG   901 TGGCCCTTAA GGTAAAAAGC ATCGTTTTCC TTATCTTTGA GATCGCAAAT CGCAGAACAT   961 TGGAAATACC CGTCCAGGCT TACATATGCA TCTGGGTGTG TACCAAAATG GCCTCTGGGC 1021 TCATATTCAC AACTGGCATA ACCAGTGCCA GTCGAGATAG TTATAACAAG G //
Primer: f1ori_R3 Sequence file: RDB14460_A6L7e.seq
>06190_14460_A6L7_f1ori_R3_E01_13_ABI24.ab1     1 GGCATGGTCT TGAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA    61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT   121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA   181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA   241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA   301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT   361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC   421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT   481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA   541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG   601 GCCCAACCTT CCAATTCATT AAATCGAGTC CTCCATCAGA TGATTTACAA GGTTTTGCAT   661 AGAATCCCTA AGTAAGAGAA GTTGTTAGTG ATTTCTTCAA GTTTACTAAA ATTTACATAC   721 AAATGGATGA TCTCTTCTCC ATTGTTTCCT TTCTTCTTGC AAACGCGTTT TACAAAGAGA   781 TGACATGCTA TTTTCTGATT ATTTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA   841 GTCCGAAATG TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA   901 GATAAAAAGT ACGGGCAAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATATTATAG   961 CCTAAAAATG TATTTTAATT GTATTTTTTG TATTTATTTC TCAATGTTTA CTTAAATGGA  1021 TGCGAATAGA GAAACTCCAC GAGTGTCAGA CTAGCTCAAG CTAAGTTATA CGAAGTTTTT 1081 ACG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl