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pDUAL-GFH81

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06190
Resource name pDUAL-GFH81
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364057.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06190 pDUAL-GFH81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06190z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14460_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14460_A6L7a.seq
>06190_14460_A6L7_nmt1Fw_A01_01_ABI24.ab1
    1 TACATTTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT
   61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA
  121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATGGAA
  181 GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA TCTCATTCTC
  241 ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA TCCGGATATC
  301 ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT
  361 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA
  421 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT
  481 GTCACTACTT TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG
  541 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC
  601 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT
  661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA
  721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA
  781 ATCAAAGTTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGGAAGCGTT TCAACTAGCA
  841 GACCATTTAT CAACAAAAAT ACTCCAATTT GGCGATGGGC CCTGTCCTTT TACCAGACAC
  901 CATTTACCCT GTCCACAACA ATCTGCCCCT TTCGAAAGAT CCCAAACGGA AAAGAGAGAC
  961 ACATGGTCCT TCTTGGAGTT GTACAGCTGC TTGGATACAC ATGCATGATG ACCTATACAA
1021 TCCATGGACT TACAGGAACG ACGATGACTA CCATACTAGC GATCACTACT GGAGATA
//
Primer: ADH_rev RDB14460_A6L7b.seq
>06190_14460_A6L7_ADH_rev_B01_04_ABI24.ab1
    1 GGGAGAGTTC TCAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
  121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG
  181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG TTTGTATAGT TCATCCATGC CATGTGTAAT
  241 CCCAGCAGCT GTTACAAACT CAAGAAGGAC CATGTGGTCT CTCTTTTCGT TGGGATCTTT
  301 CGAAAGGGCA GATTGTGTGG ACAGGTAATG GTTGTCTGGT AAAAGGACAG GGCCATCGCC
  361 AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG CTTCCATCTT CAATGTTGTG
  421 TCTAATTTTG AAGTTAACTT TGATTCCATT CTTTTGTTTG TCTGCCATGA TGTATACATT
  481 GTGTGAGTTA TAGTTGTATT CCAATTTGTG TCCAAGAATG TTTCCATCTT CTTTAAAATC
  541 AATACCTTTT AACTCGATTC TATTAACAAG GGTATCACCT TCAAACTTGA CTTCAGCACG
  601 TGTCTTGTAG TTCCCGTCAT CTTTGAAAAA TATAGTTCTT TCCTGTACAT AGCCTTCGGG
  661 CATGGCACTC TTGAAAAAGT CATGCTGTTT CATGTGATCT GGGTATCTTG AAAAGCATTG
  721 AACACCATAA CAGAAAGTAG TGACAAGTGT TGGCCACGGA AACAGGTAGT TTTCCAGTAG
  781 TGCAAATAAA TTTAAAGGGT AAGTTTTCCG TATGTTGCAT CACCTTCACC CTCTCCACTG
  841 ACAGAAAATT TGTGCCATTA ACATCACCCA TCTAATTTCA ACAAGAATTG GGGACACTCC
  901 CAGTTGAAAG TTCTCTCCTT TTACTCATGA TATCCCGGAT CCAGGGCCTG TCGACGCTTA
  961 GGCTTTACAA AGCGAACTAT TAGGTCAGAA GGTGGAGAAT TGGAGATTGG AATTAATATC
1021 AACTTATCAT TGATGAATAT TGGCCAAGGC ATC
//
Primer: ADH-Forward RDB14460_A6L7c.seq
>06190_14460_A6L7_ADH-Forward_C01_07_ABI24.ab1
    1 TGGTCATTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
   61 AGTAAGATGA TTCTTTTATC GTCGATAACG ATGAGGATGT AGACGATTAT GATGAGAGTG
  121 ATTAGCTTCT AAAATTTGGT GTTTTCAAAC GCTATTTACA GTACAATCTG AAAAAATTGG
  181 ATGAACATTG TAAATGGTAG GATACGAGTT CCAATTAATG TATTTTTTAA GTATTACACT
  241 TAGCTTATTA ATTTTATATA TACATTCTAC CTCTGTGGGT TTAAAGACAA CTCGTACACA
  301 TACTCAAATT ATTTATGAAC ATTATAATGC ATATTCGTAC TGTCTATTAA ACATTTTTTT
  361 GACATTAAAA ATTTCGTTTA CTAACGTAGA AAGCGCTGAT AATTGAGGCT ACTATTTTAA
  421 TATCTAGAAA CAAAGATTCA TTAATTAATT AAGTAAGCTC CAGGATACTT GTATATTTCG
  481 TTAAAAAAGC GCAATTTCAA CAATTCCTAT GAACATCCAT GATTTCATCA CAAAATCAAA
  541 CATATCAGGA TTTAACGTGA ATTATTTGCT TTCTTGTGAT TTTCATCTGA TTTCTGGTCA
  601 TTTACGTTAC TGTAAACAAG CTTTTTAAAA AAAGGATTTT TTACGCAATA TTATCGATAT
  661 CCCAATCTGT AGTATAAAAA GTGCAACAGT GTTGAGTTTC CCGAAACCAG GCATTTTTGC
  721 GGTAGGGTCT GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT
  781 GCAAAGAAAA TCGTCGTCTT ACCAGGAGAC CATATTGGGC CCTGAAATTG TTGCTTCTGC
  841 CTTGGAGGTT TTGAAAGTCG TTGAGAAGAA GCGACCTGAG TTAAAACTCG AGTTTGAAGA
  901 ACACAAGATT GGAGTGCCTC TATTGATGCC TATGGGAACC CCTTTGACTG ACGAGACTGT
  961 GAAGGCTTGT TGAAGCTGAC GGTGTTCCTT TTGGGTGCGG TGTGTCTGAA TGACACCCAT
 1021 GTCGTCTGAG CCAGGTTAAT GAGCCTCGTT ACAGTATGCG TGTTTCGGCC TGATCCATCC
1081 GACTTTGCCG ACT
//
Primer: ars_R1 RDB14460_A6L7d.seq
>06190_14460_A6L7_ars_R1_D01_10_ABI24.ab1
    1 TGGTTCGTTT ATACTAATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT
   61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA
  121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT
  181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG
  241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA
  301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC
  361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG
  421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT
  481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC
  541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT
  601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA
  661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG
  721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT
  781 TGACGAAGAA ATCCGAAATC TTAGAATTTG GTAGATAAAA TTGGACCCTA TGTCTGTGTT
  841 ATCAAGACAC ATATTGACGT TGTCGAGGAT TTCGACCAGG ATATGGGTAG AAAAAACTGG
  901 TGGCCCTTAA GGTAAAAAGC ATCGTTTTCC TTATCTTTGA GATCGCAAAT CGCAGAACAT
  961 TGGAAATACC CGTCCAGGCT TACATATGCA TCTGGGTGTG TACCAAAATG GCCTCTGGGC
1021 TCATATTCAC AACTGGCATA ACCAGTGCCA GTCGAGATAG TTATAACAAG G
//
Primer: f1ori_R3 RDB14460_A6L7e.seq
>06190_14460_A6L7_f1ori_R3_E01_13_ABI24.ab1
    1 GGCATGGTCT TGAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
   61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
  121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
  181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
  241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
  301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
  361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
  421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
  481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
  541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
  601 GCCCAACCTT CCAATTCATT AAATCGAGTC CTCCATCAGA TGATTTACAA GGTTTTGCAT
  661 AGAATCCCTA AGTAAGAGAA GTTGTTAGTG ATTTCTTCAA GTTTACTAAA ATTTACATAC
  721 AAATGGATGA TCTCTTCTCC ATTGTTTCCT TTCTTCTTGC AAACGCGTTT TACAAAGAGA
  781 TGACATGCTA TTTTCTGATT ATTTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA
  841 GTCCGAAATG TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA
  901 GATAAAAAGT ACGGGCAAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATATTATAG
  961 CCTAAAAATG TATTTTAATT GTATTTTTTG TATTTATTTC TCAATGTTTA CTTAAATGGA
 1021 TGCGAATAGA GAAACTCCAC GAGTGTCAGA CTAGCTCAAG CTAAGTTATA CGAAGTTTTT
1081 ACG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl