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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-GFH81

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06190
Resource name pDUAL-GFH81
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364057.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06190 pDUAL-GFH81 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06190z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14460_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14460_A6L7a.seq check
            >06190_14460_A6L7_nmt1Fw_A01_01_ABI24.ab1
                1 TACATTTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT
               61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA
              121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATGGAA
              181 GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA TCTCATTCTC
              241 ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA TCCGGATATC
              301 ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT
              361 GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA
              421 AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT
              481 GTCACTACTT TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG
              541 CATGACTTTT TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC
              601 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT
              661 AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA
              721 TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA
              781 ATCAAAGTTT AACTTCAAAA TTAGACACAA CATTGAAGAT GGGAAGCGTT TCAACTAGCA
              841 GACCATTTAT CAACAAAAAT ACTCCAATTT GGCGATGGGC CCTGTCCTTT TACCAGACAC
              901 CATTTACCCT GTCCACAACA ATCTGCCCCT TTCGAAAGAT CCCAAACGGA AAAGAGAGAC
              961 ACATGGTCCT TCTTGGAGTT GTACAGCTGC TTGGATACAC ATGCATGATG ACCTATACAA
            1021 TCCATGGACT TACAGGAACG ACGATGACTA CCATACTAGC GATCACTACT GGAGATA
            //
            Primer: ADH_rev
            Sequence file: RDB14460_A6L7b.seq check
            >06190_14460_A6L7_ADH_rev_B01_04_ABI24.ab1
                1 GGGAGAGTTC TCAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
              121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CAGTGGTGAT GGTGGTGATG
              181 TTTGTCATCG TCGTCCTTGT AGTCCATGGG TTTGTATAGT TCATCCATGC CATGTGTAAT
              241 CCCAGCAGCT GTTACAAACT CAAGAAGGAC CATGTGGTCT CTCTTTTCGT TGGGATCTTT
              301 CGAAAGGGCA GATTGTGTGG ACAGGTAATG GTTGTCTGGT AAAAGGACAG GGCCATCGCC
              361 AATTGGAGTA TTTTGTTGAT AATGGTCTGC TAGTTGAACG CTTCCATCTT CAATGTTGTG
              421 TCTAATTTTG AAGTTAACTT TGATTCCATT CTTTTGTTTG TCTGCCATGA TGTATACATT
              481 GTGTGAGTTA TAGTTGTATT CCAATTTGTG TCCAAGAATG TTTCCATCTT CTTTAAAATC
              541 AATACCTTTT AACTCGATTC TATTAACAAG GGTATCACCT TCAAACTTGA CTTCAGCACG
              601 TGTCTTGTAG TTCCCGTCAT CTTTGAAAAA TATAGTTCTT TCCTGTACAT AGCCTTCGGG
              661 CATGGCACTC TTGAAAAAGT CATGCTGTTT CATGTGATCT GGGTATCTTG AAAAGCATTG
              721 AACACCATAA CAGAAAGTAG TGACAAGTGT TGGCCACGGA AACAGGTAGT TTTCCAGTAG
              781 TGCAAATAAA TTTAAAGGGT AAGTTTTCCG TATGTTGCAT CACCTTCACC CTCTCCACTG
              841 ACAGAAAATT TGTGCCATTA ACATCACCCA TCTAATTTCA ACAAGAATTG GGGACACTCC
              901 CAGTTGAAAG TTCTCTCCTT TTACTCATGA TATCCCGGAT CCAGGGCCTG TCGACGCTTA
              961 GGCTTTACAA AGCGAACTAT TAGGTCAGAA GGTGGAGAAT TGGAGATTGG AATTAATATC
            1021 AACTTATCAT TGATGAATAT TGGCCAAGGC ATC
            //
            Primer: ADH-Forward
            Sequence file: RDB14460_A6L7c.seq check
            >06190_14460_A6L7_ADH-Forward_C01_07_ABI24.ab1
                1 TGGTCATTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
               61 AGTAAGATGA TTCTTTTATC GTCGATAACG ATGAGGATGT AGACGATTAT GATGAGAGTG
              121 ATTAGCTTCT AAAATTTGGT GTTTTCAAAC GCTATTTACA GTACAATCTG AAAAAATTGG
              181 ATGAACATTG TAAATGGTAG GATACGAGTT CCAATTAATG TATTTTTTAA GTATTACACT
              241 TAGCTTATTA ATTTTATATA TACATTCTAC CTCTGTGGGT TTAAAGACAA CTCGTACACA
              301 TACTCAAATT ATTTATGAAC ATTATAATGC ATATTCGTAC TGTCTATTAA ACATTTTTTT
              361 GACATTAAAA ATTTCGTTTA CTAACGTAGA AAGCGCTGAT AATTGAGGCT ACTATTTTAA
              421 TATCTAGAAA CAAAGATTCA TTAATTAATT AAGTAAGCTC CAGGATACTT GTATATTTCG
              481 TTAAAAAAGC GCAATTTCAA CAATTCCTAT GAACATCCAT GATTTCATCA CAAAATCAAA
              541 CATATCAGGA TTTAACGTGA ATTATTTGCT TTCTTGTGAT TTTCATCTGA TTTCTGGTCA
              601 TTTACGTTAC TGTAAACAAG CTTTTTAAAA AAAGGATTTT TTACGCAATA TTATCGATAT
              661 CCCAATCTGT AGTATAAAAA GTGCAACAGT GTTGAGTTTC CCGAAACCAG GCATTTTTGC
              721 GGTAGGGTCT GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT
              781 GCAAAGAAAA TCGTCGTCTT ACCAGGAGAC CATATTGGGC CCTGAAATTG TTGCTTCTGC
              841 CTTGGAGGTT TTGAAAGTCG TTGAGAAGAA GCGACCTGAG TTAAAACTCG AGTTTGAAGA
              901 ACACAAGATT GGAGTGCCTC TATTGATGCC TATGGGAACC CCTTTGACTG ACGAGACTGT
              961 GAAGGCTTGT TGAAGCTGAC GGTGTTCCTT TTGGGTGCGG TGTGTCTGAA TGACACCCAT
             1021 GTCGTCTGAG CCAGGTTAAT GAGCCTCGTT ACAGTATGCG TGTTTCGGCC TGATCCATCC
            1081 GACTTTGCCG ACT
            //
            Primer: ars_R1
            Sequence file: RDB14460_A6L7d.seq check
            >06190_14460_A6L7_ars_R1_D01_10_ABI24.ab1
                1 TGGTTCGTTT ATACTAATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT
               61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA
              121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT
              181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG
              241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA
              301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC
              361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG
              421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT
              481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC
              541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT
              601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA
              661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG
              721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT
              781 TGACGAAGAA ATCCGAAATC TTAGAATTTG GTAGATAAAA TTGGACCCTA TGTCTGTGTT
              841 ATCAAGACAC ATATTGACGT TGTCGAGGAT TTCGACCAGG ATATGGGTAG AAAAAACTGG
              901 TGGCCCTTAA GGTAAAAAGC ATCGTTTTCC TTATCTTTGA GATCGCAAAT CGCAGAACAT
              961 TGGAAATACC CGTCCAGGCT TACATATGCA TCTGGGTGTG TACCAAAATG GCCTCTGGGC
            1021 TCATATTCAC AACTGGCATA ACCAGTGCCA GTCGAGATAG TTATAACAAG G
            //
            Primer: f1ori_R3
            Sequence file: RDB14460_A6L7e.seq check
            >06190_14460_A6L7_f1ori_R3_E01_13_ABI24.ab1
                1 GGCATGGTCT TGAGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
               61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
              121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
              181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
              241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
              301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
              361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
              421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
              481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
              541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
              601 GCCCAACCTT CCAATTCATT AAATCGAGTC CTCCATCAGA TGATTTACAA GGTTTTGCAT
              661 AGAATCCCTA AGTAAGAGAA GTTGTTAGTG ATTTCTTCAA GTTTACTAAA ATTTACATAC
              721 AAATGGATGA TCTCTTCTCC ATTGTTTCCT TTCTTCTTGC AAACGCGTTT TACAAAGAGA
              781 TGACATGCTA TTTTCTGATT ATTTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA
              841 GTCCGAAATG TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA
              901 GATAAAAAGT ACGGGCAAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATATTATAG
              961 CCTAAAAATG TATTTTAATT GTATTTTTTG TATTTATTTC TCAATGTTTA CTTAAATGGA
             1021 TGCGAATAGA GAAACTCCAC GAGTGTCAGA CTAGCTCAAG CTAAGTTATA CGAAGTTTTT
            1081 ACG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl