RDB06189 - pDUAL-GFH41

Resource data sheet
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pDUAL-GFH41

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number	RDB06189
Resource name	pDUAL-GFH41
Clone info.	S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364056.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	【追加提供依頼書】GFP-A Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06189	pDUAL-GFH41	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06189z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14459_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw	RDB14459_A6L7a.seq
>06189_14459_A6L7_nmt1Fw_F03_06_ABI08.ab1 1 TGAAAATTTA TTTAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA 61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT 121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA 181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC 241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC 301 CGGATATCAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 361 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 421 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCGTGGC 481 CAACACTTGT CACTACTTTC TGTTATGGTG TTCAATGCTT TTCAAGATAC CCAGATCACA 541 TGAAACAGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG CTATGTACAG GAAAGAACTA 601 TATTTTTCAA AGATGACGGG AACTACAAGA CACGTGCTGA AGTCAAGTTT GAAGGTGATA 661 CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG 721 GACACAAATT GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA 781 AGAATGGAAT CAAAGTTAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC 841 TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCCT TTTACCAGAC 901 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCACGAAAAG AGAGACCACA 961 TGGGTTCCTT CTGAGTTTGT AACAGCTGCT GGGATACACA TTGGCATGGA TGACTATACC 1021 AACCATGGAC TTACAGACGA CGATGACCAA CATCACCACC AATCACTACT GGAATCTCGA 1081 ACTCTAGAAG ATCAGACGCG TCCACAT //
Primer: ADH_rev	RDB14459_A6L7b.seq
>06189_14459_A6L7_ADH_rev_G03_07_ABI08.ab1 1 GGGGGATTAT CAAGGATAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT 61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT 121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT 181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA 241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT 301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC 361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT 421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT 481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT 541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC 601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG 661 GCATGGCACT CTTGAAAAAG TCATGCTGTT TCATGTGATC TGGGTATCTT GAAAAGCATT 721 GAACACCATA ACAGAAAGTA GTGACAAGTG TTGGCCACGG AACAGGTAGT TTTCCAGTAG 781 TGCAAATAAA TTTAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA 841 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG 901 TGAAAAAGTT CTTTCTCCTT TACTCATGAT ATCCGGATCC CAGGCCCTGT CGACGCTAGC 961 TTTAACAAAG CGACTATAAG TCAGAAGTGA AGATGAGATT TGAAATATTT AATTCACTTA 1021 ATTCCATTGA ATGGATATGC CCAGGAATCG TCGTCCGATT TATCTTCGAA GGTTATCCAA 1081 GTTAC //
Primer: ADH-Forward	RDB14459_A6L7c.seq
>06189_14459_A6L7_ADH-Forward_H03_08_ABI08.ab1 1 AATTCCCCTT TAATGGAACA CACCTCTACC GGCAGATCCG CTAGGGATAA CAGGGTAATA 61 TACTAGTAAG ATGATTCTTT TATCGTCGAT AACGATGAGG ATGTAGACGA TTATGATGAG 121 AGTGATTAGC TTCTAAAATT TGGTGTTTTC AAACGCTATT TACAGTACAA TCTGAAAAAA 181 TTGGATGAAC ATTGTAAATG GTAGGATACG AGTTCCAATT AATGTATTTT TTAAGTATTA 241 CACTTAGCTT ATTAATTTTA TATATACATT CTACCTCTGT GGGTTTAAAG ACAACTCGTA 301 CACATACTCA AATTATTTAT GAACATTATA ATGCATATTC GTACTGTCTA TTAAACATTT 361 TTTTGACATT AAAAATTTCG TTTACTAACG TAGAAAGCGC TGATAATTGA GGCTACTATT 421 TTAATATCTA GAAACAAAGA TTCATTAATT AATTAAGTAA GCTCCAGGAT ACTTGTATAT 481 TTCGTTAAAA AAGCGCAATT TCAACAATTC CTATGAACAT CCATGATTTC ATCACAAAAT 541 CAAACATATC AGGATTTAAC GTGAATTATT TGCTTTCTTG TGATTTTCAT CTGATTTCTG 601 GTCATTTACG TTACTGTAAA CAAGCTTTTT AAAAAAAGGA TTTTTTACGC AATATTATCG 661 ATATCCCAAT CTGTAGTATA AAAAGTGCAA CAGTGTTGAG TTTCCCGAAA CCAGGCATTT 721 TTGCGGTAGG GTCTGAAACT TTCACGAAAC CATTTCACAA CAAACTTCTT CAACAACATG 781 TGTGCAAAGA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAATTG TTGCTTCTGC 841 CTTGGAGGTT TTGAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTGAGACAC 901 AAGATTTGAG GTGCCTCTAT GATGCCTATG AACCCCTTTG ACTGACGAGA CTGTGAGGCT 961 TGTTTGGAGC CTGACGTGTT CTTTGGGTGC GTTGTGTCTG AATTGACACC TATGTCGTCC 1021 TGGACCAGTA TGAGCTCGGT AGAGATATGG GTTTTG //
Primer: ars_R1	RDB14459_A6L7d.seq
>06189_14459_A6L7_ars_R1_A04_01_ABI08.ab1 1 TGGTCCGATT TATACTAATT ACTTAAGTAC CTTTAACTAA ACAAAATGCC TATATATATA 61 TTAATTTACA ATGAGTGTCA GATAAGTCAC TATGTCCGAG TGGTTAAGGA GTTAGACTCG 121 AATTCGCAAG CTTAGCTACA AATCCCACTG GCTATATGTA TGCATTTGTG TTAAAAAAGT 181 TTGTATAGAT TATTTAATCT ACTCAGCATT CTTTCTCTAA ATAGGAATTT GTTACTTAAT 241 GGAGAAAAAA ATGTTTCGAT TTACCTAGTG TATTTGTTTG TATACTCACG TTTAATTTCA 301 AACATCCATT CTATCTTGTG TAATTTTTGG CATGGTGAAA AAGATAATCA GCCTTATAAT 361 CTTTACAAAA GTAAGAAATT CTGTAAATAA GCCTTAATGC CCTTGCTTTA AATTAAAATG 421 GTTCTTTTTC ATGATAATGT TTGCACTTTG TGAATATATT TTAGATAGTT CTGTGAGGTA 481 TAATTAAGAT GTTTTAGAGA CTTATACAAT TTTGTCTTTA TAAATTCTTA ATTGATTTTA 541 CCATCCCAGT TTAACTATGC TTCGTCGGCA TCTCTGCACA TGTCGTGTTT TCTTACCGTA 601 TTGTCCTACC AAGAACCTCT TTTTTGCTTG GATCGAAATT AAAGGTTTAA AAGCAAAGTT 661 ATGGATGCTA GAGTATTTCA AAGCTATTCA GCTAGAGCTG AGGGGATGAA AAATCCCATT 721 GCCAAGGAAT TGTTGGCTTT GATGGAAGAA AAGCAAAGCA ACTTGTCAGT CGCGGTCGAT 781 TTGACGAAGA AATCCGAAAT CTTAGAATTG GTAGATAAAA TTGGACCCTA TGTCTGTGTT 841 ATCAAGACAC ATATTGACGT TGTCGAGGAT TTCGACCAGG ATATGGTAGA AAAAACTGGT 901 GGGCCTTAGT AAAAAGCATC GTTTTCTTAT CTTTGAGGAT CGCAAATTCG CAGACATTGG 961 AAAATAACCG TCAAAGCTAC AAATAATTGC ATCTGGTGTG TACAAAAATT GCTTCCTTGG 1021 CTTCATATCA CAAAATGGCC ATAACCGATG CCAGCCGAGG GTATTATAAC AACGCTTCAA 1081 GAGTGGTTAC CTTGGACCGT GTCCTCT //
Primer: f1ori_R3	RDB14459_A6L7e.seq
>06189_14459_A6L7_f1ori_R3_B04_02_ABI08.ab1 1 GCCCTGGGGG GTGAGGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG 61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC 121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT 181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG 241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA 301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT 361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC 421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT 481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT 541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC 601 GGGCCCAACC TTCCAATTCA TTAAATCGAG TCCTCCATCA GATGATTTAC AAGGTTTTGC 661 ATAGAATCCC TAAGTAAGAG AAGTTGTTAG TGATTTCTTC AAGTTTACTA AAATTTACAT 721 ACAAATGGAT GATCTCTTCT CCATTGTTTC CTTTCTTCTT GCAAACGCGT TTTACAAAGA 781 GATGACATGC TATTTTCTGA TTATTTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTTGG 841 GAGTCCGAAA TGTAAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA 901 AAAGAATAAA AAAGTAAGGG GCAAGCGAGA CAGAATGGGA TACAAGGCAT CGTCTATAAT 961 TATAGCTAAA AATTGTATTT TAATTTGTAT TTTTTTTGTA AATTTTAATT TCTCATGGTT 1021 TTACTGAAAT GGAATGCGAG TTAGAAGAAA GCTCGACGAA TGTCTAGAAG TAGCCTCCAG 1081 TCGAAAAAAT //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report	Subramanian, L., Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization. EMBO Rep. 17 (4):496-507 (2016). PMID 26921242.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl