Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-GFH41

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14459_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14459_A6L7a.seq
>06189_14459_A6L7_nmt1Fw_F03_06_ABI08.ab1     1 TGAAAATTTA TTTAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA    61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT   121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA   181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC   241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC   301 CGGATATCAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT   361 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA   421 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCGTGGC   481 CAACACTTGT CACTACTTTC TGTTATGGTG TTCAATGCTT TTCAAGATAC CCAGATCACA   541 TGAAACAGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG CTATGTACAG GAAAGAACTA   601 TATTTTTCAA AGATGACGGG AACTACAAGA CACGTGCTGA AGTCAAGTTT GAAGGTGATA   661 CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG   721 GACACAAATT GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA   781 AGAATGGAAT CAAAGTTAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC   841 TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCCT TTTACCAGAC   901 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCACGAAAAG AGAGACCACA   961 TGGGTTCCTT CTGAGTTTGT AACAGCTGCT GGGATACACA TTGGCATGGA TGACTATACC  1021 AACCATGGAC TTACAGACGA CGATGACCAA CATCACCACC AATCACTACT GGAATCTCGA 1081 ACTCTAGAAG ATCAGACGCG TCCACAT //
Primer: ADH_rev Sequence file: RDB14459_A6L7b.seq
>06189_14459_A6L7_ADH_rev_G03_07_ABI08.ab1     1 GGGGGATTAT CAAGGATAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT    61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT   121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT   181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA   241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT   301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC   361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT   421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT   481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT   541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC   601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG   661 GCATGGCACT CTTGAAAAAG TCATGCTGTT TCATGTGATC TGGGTATCTT GAAAAGCATT   721 GAACACCATA ACAGAAAGTA GTGACAAGTG TTGGCCACGG AACAGGTAGT TTTCCAGTAG   781 TGCAAATAAA TTTAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA   841 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG   901 TGAAAAAGTT CTTTCTCCTT TACTCATGAT ATCCGGATCC CAGGCCCTGT CGACGCTAGC   961 TTTAACAAAG CGACTATAAG TCAGAAGTGA AGATGAGATT TGAAATATTT AATTCACTTA  1021 ATTCCATTGA ATGGATATGC CCAGGAATCG TCGTCCGATT TATCTTCGAA GGTTATCCAA 1081 GTTAC //
Primer: ADH-Forward Sequence file: RDB14459_A6L7c.seq
>06189_14459_A6L7_ADH-Forward_H03_08_ABI08.ab1     1 AATTCCCCTT TAATGGAACA CACCTCTACC GGCAGATCCG CTAGGGATAA CAGGGTAATA    61 TACTAGTAAG ATGATTCTTT TATCGTCGAT AACGATGAGG ATGTAGACGA TTATGATGAG   121 AGTGATTAGC TTCTAAAATT TGGTGTTTTC AAACGCTATT TACAGTACAA TCTGAAAAAA   181 TTGGATGAAC ATTGTAAATG GTAGGATACG AGTTCCAATT AATGTATTTT TTAAGTATTA   241 CACTTAGCTT ATTAATTTTA TATATACATT CTACCTCTGT GGGTTTAAAG ACAACTCGTA   301 CACATACTCA AATTATTTAT GAACATTATA ATGCATATTC GTACTGTCTA TTAAACATTT   361 TTTTGACATT AAAAATTTCG TTTACTAACG TAGAAAGCGC TGATAATTGA GGCTACTATT   421 TTAATATCTA GAAACAAAGA TTCATTAATT AATTAAGTAA GCTCCAGGAT ACTTGTATAT   481 TTCGTTAAAA AAGCGCAATT TCAACAATTC CTATGAACAT CCATGATTTC ATCACAAAAT   541 CAAACATATC AGGATTTAAC GTGAATTATT TGCTTTCTTG TGATTTTCAT CTGATTTCTG   601 GTCATTTACG TTACTGTAAA CAAGCTTTTT AAAAAAAGGA TTTTTTACGC AATATTATCG   661 ATATCCCAAT CTGTAGTATA AAAAGTGCAA CAGTGTTGAG TTTCCCGAAA CCAGGCATTT   721 TTGCGGTAGG GTCTGAAACT TTCACGAAAC CATTTCACAA CAAACTTCTT CAACAACATG   781 TGTGCAAAGA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAATTG TTGCTTCTGC   841 CTTGGAGGTT TTGAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTGAGACAC   901 AAGATTTGAG GTGCCTCTAT GATGCCTATG AACCCCTTTG ACTGACGAGA CTGTGAGGCT   961 TGTTTGGAGC CTGACGTGTT CTTTGGGTGC GTTGTGTCTG AATTGACACC TATGTCGTCC 1021 TGGACCAGTA TGAGCTCGGT AGAGATATGG GTTTTG //
Primer: ars_R1 Sequence file: RDB14459_A6L7d.seq
>06189_14459_A6L7_ars_R1_A04_01_ABI08.ab1     1 TGGTCCGATT TATACTAATT ACTTAAGTAC CTTTAACTAA ACAAAATGCC TATATATATA    61 TTAATTTACA ATGAGTGTCA GATAAGTCAC TATGTCCGAG TGGTTAAGGA GTTAGACTCG   121 AATTCGCAAG CTTAGCTACA AATCCCACTG GCTATATGTA TGCATTTGTG TTAAAAAAGT   181 TTGTATAGAT TATTTAATCT ACTCAGCATT CTTTCTCTAA ATAGGAATTT GTTACTTAAT   241 GGAGAAAAAA ATGTTTCGAT TTACCTAGTG TATTTGTTTG TATACTCACG TTTAATTTCA   301 AACATCCATT CTATCTTGTG TAATTTTTGG CATGGTGAAA AAGATAATCA GCCTTATAAT   361 CTTTACAAAA GTAAGAAATT CTGTAAATAA GCCTTAATGC CCTTGCTTTA AATTAAAATG   421 GTTCTTTTTC ATGATAATGT TTGCACTTTG TGAATATATT TTAGATAGTT CTGTGAGGTA   481 TAATTAAGAT GTTTTAGAGA CTTATACAAT TTTGTCTTTA TAAATTCTTA ATTGATTTTA   541 CCATCCCAGT TTAACTATGC TTCGTCGGCA TCTCTGCACA TGTCGTGTTT TCTTACCGTA   601 TTGTCCTACC AAGAACCTCT TTTTTGCTTG GATCGAAATT AAAGGTTTAA AAGCAAAGTT   661 ATGGATGCTA GAGTATTTCA AAGCTATTCA GCTAGAGCTG AGGGGATGAA AAATCCCATT   721 GCCAAGGAAT TGTTGGCTTT GATGGAAGAA AAGCAAAGCA ACTTGTCAGT CGCGGTCGAT   781 TTGACGAAGA AATCCGAAAT CTTAGAATTG GTAGATAAAA TTGGACCCTA TGTCTGTGTT   841 ATCAAGACAC ATATTGACGT TGTCGAGGAT TTCGACCAGG ATATGGTAGA AAAAACTGGT   901 GGGCCTTAGT AAAAAGCATC GTTTTCTTAT CTTTGAGGAT CGCAAATTCG CAGACATTGG   961 AAAATAACCG TCAAAGCTAC AAATAATTGC ATCTGGTGTG TACAAAAATT GCTTCCTTGG  1021 CTTCATATCA CAAAATGGCC ATAACCGATG CCAGCCGAGG GTATTATAAC AACGCTTCAA 1081 GAGTGGTTAC CTTGGACCGT GTCCTCT //
Primer: f1ori_R3 Sequence file: RDB14459_A6L7e.seq
>06189_14459_A6L7_f1ori_R3_B04_02_ABI08.ab1     1 GCCCTGGGGG GTGAGGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG    61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC   121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT   181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG   241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA   301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT   361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC   421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT   481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT   541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC   601 GGGCCCAACC TTCCAATTCA TTAAATCGAG TCCTCCATCA GATGATTTAC AAGGTTTTGC   661 ATAGAATCCC TAAGTAAGAG AAGTTGTTAG TGATTTCTTC AAGTTTACTA AAATTTACAT   721 ACAAATGGAT GATCTCTTCT CCATTGTTTC CTTTCTTCTT GCAAACGCGT TTTACAAAGA   781 GATGACATGC TATTTTCTGA TTATTTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTTGG   841 GAGTCCGAAA TGTAAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA   901 AAAGAATAAA AAAGTAAGGG GCAAGCGAGA CAGAATGGGA TACAAGGCAT CGTCTATAAT   961 TATAGCTAAA AATTGTATTT TAATTTGTAT TTTTTTTGTA AATTTTAATT TCTCATGGTT  1021 TTACTGAAAT GGAATGCGAG TTAGAAGAAA GCTCGACGAA TGTCTAGAAG TAGCCTCCAG 1081 TCGAAAAAAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl