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pDUAL-GFH41

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06189
Resource name pDUAL-GFH41
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364056.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06189 pDUAL-GFH41 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06189z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14459_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14459_A6L7a.seq
>06189_14459_A6L7_nmt1Fw_F03_06_ABI08.ab1
    1 TGAAAATTTA TTTAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC
  241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC
  301 CGGATATCAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT
  361 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA
  421 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCGTGGC
  481 CAACACTTGT CACTACTTTC TGTTATGGTG TTCAATGCTT TTCAAGATAC CCAGATCACA
  541 TGAAACAGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG CTATGTACAG GAAAGAACTA
  601 TATTTTTCAA AGATGACGGG AACTACAAGA CACGTGCTGA AGTCAAGTTT GAAGGTGATA
  661 CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG
  721 GACACAAATT GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA
  781 AGAATGGAAT CAAAGTTAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC
  841 TAGCAGACCA TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCCT TTTACCAGAC
  901 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCACGAAAAG AGAGACCACA
  961 TGGGTTCCTT CTGAGTTTGT AACAGCTGCT GGGATACACA TTGGCATGGA TGACTATACC
 1021 AACCATGGAC TTACAGACGA CGATGACCAA CATCACCACC AATCACTACT GGAATCTCGA
1081 ACTCTAGAAG ATCAGACGCG TCCACAT
//
Primer: ADH_rev RDB14459_A6L7b.seq
>06189_14459_A6L7_ADH_rev_G03_07_ABI08.ab1
    1 GGGGGATTAT CAAGGATAGA ATTTTCGTTT TAAACCTAAG AGTCACTTTA AAATTTGTAT
   61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT
  121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT
  181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GTTTGTATAG TTCATCCATG CCATGTGTAA
  241 TCCCAGCAGC TGTTACAAAC TCAAGAAGGA CCATGTGGTC TCTCTTTTCG TTGGGATCTT
  301 TCGAAAGGGC AGATTGTGTG GACAGGTAAT GGTTGTCTGG TAAAAGGACA GGGCCATCGC
  361 CAATTGGAGT ATTTTGTTGA TAATGGTCTG CTAGTTGAAC GCTTCCATCT TCAATGTTGT
  421 GTCTAATTTT GAAGTTAACT TTGATTCCAT TCTTTTGTTT GTCTGCCATG ATGTATACAT
  481 TGTGTGAGTT ATAGTTGTAT TCCAATTTGT GTCCAAGAAT GTTTCCATCT TCTTTAAAAT
  541 CAATACCTTT TAACTCGATT CTATTAACAA GGGTATCACC TTCAAACTTG ACTTCAGCAC
  601 GTGTCTTGTA GTTCCCGTCA TCTTTGAAAA ATATAGTTCT TTCCTGTACA TAGCCTTCGG
  661 GCATGGCACT CTTGAAAAAG TCATGCTGTT TCATGTGATC TGGGTATCTT GAAAAGCATT
  721 GAACACCATA ACAGAAAGTA GTGACAAGTG TTGGCCACGG AACAGGTAGT TTTCCAGTAG
  781 TGCAAATAAA TTTAAGGGTA AGTTTTCCGT ATGTTGCATC ACCTTCACCC TCTCCACTGA
  841 CAGAAAATTT GTGCCCATTA ACATCACCAT CTAATTCAAC AAGAATTGGG ACAACTCCAG
  901 TGAAAAAGTT CTTTCTCCTT TACTCATGAT ATCCGGATCC CAGGCCCTGT CGACGCTAGC
  961 TTTAACAAAG CGACTATAAG TCAGAAGTGA AGATGAGATT TGAAATATTT AATTCACTTA
 1021 ATTCCATTGA ATGGATATGC CCAGGAATCG TCGTCCGATT TATCTTCGAA GGTTATCCAA
1081 GTTAC
//
Primer: ADH-Forward RDB14459_A6L7c.seq
>06189_14459_A6L7_ADH-Forward_H03_08_ABI08.ab1
    1 AATTCCCCTT TAATGGAACA CACCTCTACC GGCAGATCCG CTAGGGATAA CAGGGTAATA
   61 TACTAGTAAG ATGATTCTTT TATCGTCGAT AACGATGAGG ATGTAGACGA TTATGATGAG
  121 AGTGATTAGC TTCTAAAATT TGGTGTTTTC AAACGCTATT TACAGTACAA TCTGAAAAAA
  181 TTGGATGAAC ATTGTAAATG GTAGGATACG AGTTCCAATT AATGTATTTT TTAAGTATTA
  241 CACTTAGCTT ATTAATTTTA TATATACATT CTACCTCTGT GGGTTTAAAG ACAACTCGTA
  301 CACATACTCA AATTATTTAT GAACATTATA ATGCATATTC GTACTGTCTA TTAAACATTT
  361 TTTTGACATT AAAAATTTCG TTTACTAACG TAGAAAGCGC TGATAATTGA GGCTACTATT
  421 TTAATATCTA GAAACAAAGA TTCATTAATT AATTAAGTAA GCTCCAGGAT ACTTGTATAT
  481 TTCGTTAAAA AAGCGCAATT TCAACAATTC CTATGAACAT CCATGATTTC ATCACAAAAT
  541 CAAACATATC AGGATTTAAC GTGAATTATT TGCTTTCTTG TGATTTTCAT CTGATTTCTG
  601 GTCATTTACG TTACTGTAAA CAAGCTTTTT AAAAAAAGGA TTTTTTACGC AATATTATCG
  661 ATATCCCAAT CTGTAGTATA AAAAGTGCAA CAGTGTTGAG TTTCCCGAAA CCAGGCATTT
  721 TTGCGGTAGG GTCTGAAACT TTCACGAAAC CATTTCACAA CAAACTTCTT CAACAACATG
  781 TGTGCAAAGA AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAATTG TTGCTTCTGC
  841 CTTGGAGGTT TTGAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTGAGACAC
  901 AAGATTTGAG GTGCCTCTAT GATGCCTATG AACCCCTTTG ACTGACGAGA CTGTGAGGCT
  961 TGTTTGGAGC CTGACGTGTT CTTTGGGTGC GTTGTGTCTG AATTGACACC TATGTCGTCC
1021 TGGACCAGTA TGAGCTCGGT AGAGATATGG GTTTTG
//
Primer: ars_R1 RDB14459_A6L7d.seq
>06189_14459_A6L7_ars_R1_A04_01_ABI08.ab1
    1 TGGTCCGATT TATACTAATT ACTTAAGTAC CTTTAACTAA ACAAAATGCC TATATATATA
   61 TTAATTTACA ATGAGTGTCA GATAAGTCAC TATGTCCGAG TGGTTAAGGA GTTAGACTCG
  121 AATTCGCAAG CTTAGCTACA AATCCCACTG GCTATATGTA TGCATTTGTG TTAAAAAAGT
  181 TTGTATAGAT TATTTAATCT ACTCAGCATT CTTTCTCTAA ATAGGAATTT GTTACTTAAT
  241 GGAGAAAAAA ATGTTTCGAT TTACCTAGTG TATTTGTTTG TATACTCACG TTTAATTTCA
  301 AACATCCATT CTATCTTGTG TAATTTTTGG CATGGTGAAA AAGATAATCA GCCTTATAAT
  361 CTTTACAAAA GTAAGAAATT CTGTAAATAA GCCTTAATGC CCTTGCTTTA AATTAAAATG
  421 GTTCTTTTTC ATGATAATGT TTGCACTTTG TGAATATATT TTAGATAGTT CTGTGAGGTA
  481 TAATTAAGAT GTTTTAGAGA CTTATACAAT TTTGTCTTTA TAAATTCTTA ATTGATTTTA
  541 CCATCCCAGT TTAACTATGC TTCGTCGGCA TCTCTGCACA TGTCGTGTTT TCTTACCGTA
  601 TTGTCCTACC AAGAACCTCT TTTTTGCTTG GATCGAAATT AAAGGTTTAA AAGCAAAGTT
  661 ATGGATGCTA GAGTATTTCA AAGCTATTCA GCTAGAGCTG AGGGGATGAA AAATCCCATT
  721 GCCAAGGAAT TGTTGGCTTT GATGGAAGAA AAGCAAAGCA ACTTGTCAGT CGCGGTCGAT
  781 TTGACGAAGA AATCCGAAAT CTTAGAATTG GTAGATAAAA TTGGACCCTA TGTCTGTGTT
  841 ATCAAGACAC ATATTGACGT TGTCGAGGAT TTCGACCAGG ATATGGTAGA AAAAACTGGT
  901 GGGCCTTAGT AAAAAGCATC GTTTTCTTAT CTTTGAGGAT CGCAAATTCG CAGACATTGG
  961 AAAATAACCG TCAAAGCTAC AAATAATTGC ATCTGGTGTG TACAAAAATT GCTTCCTTGG
 1021 CTTCATATCA CAAAATGGCC ATAACCGATG CCAGCCGAGG GTATTATAAC AACGCTTCAA
1081 GAGTGGTTAC CTTGGACCGT GTCCTCT
//
Primer: f1ori_R3 RDB14459_A6L7e.seq
>06189_14459_A6L7_f1ori_R3_B04_02_ABI08.ab1
    1 GCCCTGGGGG GTGAGGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
   61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG
  241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA
  301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT
  361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC
  421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT
  481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT
  541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC
  601 GGGCCCAACC TTCCAATTCA TTAAATCGAG TCCTCCATCA GATGATTTAC AAGGTTTTGC
  661 ATAGAATCCC TAAGTAAGAG AAGTTGTTAG TGATTTCTTC AAGTTTACTA AAATTTACAT
  721 ACAAATGGAT GATCTCTTCT CCATTGTTTC CTTTCTTCTT GCAAACGCGT TTTACAAAGA
  781 GATGACATGC TATTTTCTGA TTATTTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTTGG
  841 GAGTCCGAAA TGTAAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA
  901 AAAGAATAAA AAAGTAAGGG GCAAGCGAGA CAGAATGGGA TACAAGGCAT CGTCTATAAT
  961 TATAGCTAAA AATTGTATTT TAATTTGTAT TTTTTTTGTA AATTTTAATT TCTCATGGTT
 1021 TTACTGAAAT GGAATGCGAG TTAGAAGAAA GCTCGACGAA TGTCTAGAAG TAGCCTCCAG
1081 TCGAAAAAAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report Subramanian, L., Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization. EMBO Rep. 17 (4):496-507 (2016). PMID 26921242.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl