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pDUAL-GFH1

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06188
Resource name pDUAL-GFH1
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364055.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06188 pDUAL-GFH1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06188z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14458_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14458_A6L7a.seq
>06188_14458_A6L7_nmt1Fw_A03_01_ABI08.ab1
    1 TAAATTTTAA TTAATCAGGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
  241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
  301 ATCCGGATAT CATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG
  361 AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG
  421 CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCGT
  481 GGCCAACACT TGTCACTACT TTCTGTTATG GTGTTCAATG CTTTTCAAGA TACCCAGATC
  541 ACATGAAACA GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGCTATGTA CAGGAAAGAA
  601 CTATATTTTT CAAAGATGAC GGGAACTACA AGACACGTGC TGAAGTCAAG TTTGAAGGTG
  661 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC
  721 TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC
  781 AAAAGAATGG GAATCAAAGT TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT
  841 CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA
  901 GACAACCATT ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC
  961 CACATGGGTC CTTTCTTGAG TTTGTAACAG CCTGCTGGGA TTACACATGG CATGGGATGG
 1021 ACTATACAAC CCATGGACTA CCAGGACGAC GATGAACTAA CCATCACACC ATCACCACTT
1081 GGAGATCTCG ACTCCTAGAA GGAATTCCCG
//
Primer: ADH_rev RDB14458_A6L7b.seq
>06188_14458_A6L7_ADH_rev_B03_02_ABI08.ab1
    1 GGGGGGTTAA TCAAGGAATA AGAATTTTCG TTTTAAACCT AAGGAGGTCA CTTTAAAATT
   61 TGGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA
  121 TTCGCTTATT TAGAAGTGGC GCGCCGGATC CTCTAGAGTC GAGATCTCAG TGGTGATGGT
  181 GGTGATGTTT GTCATCGTCG TCCTTGTAGT CCATGGGTTT GTATAGTTCA TCCATGCCAT
  241 GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT GTGGTCTCTC TTTTCGTTGG
  301 GATCTTTCGA AAGGGCAGAT TGTGTGGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC
  361 CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGCTT CCATCTTCAA
  421 TGTTGTGTCT AATTTTGAAG TTAACTTTGA TTCCATTCTT TTGTTTGTCT GCCATGATGT
  481 ATACATTGTG TGAGTTATAG TTGTATTCCA ATTTGTGTCC AAGAATGTTT CCATCTTCTT
  541 TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT ATCACCTTCA AACTTGACTT
  601 CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT AGTTCTTTCC TGTACATAGC
  661 CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCTGTTTCAT GTGATCTGGG TATCTTGAAA
  721 AGCATTGAAC ACCATAACAG AAAGTAGTGA CAAGTGTTGG CCACGGAACA GGTAGTTTTC
  781 CAGTAGTGCA AATAAATTTA AGGGTAAGTT TTCCGTATGT TGCATCACCT TCACCCTCTC
  841 CACTGACAGA AAATTTTGTG CCCATTAACA TCACCATCTA AATTCAACAA GAATTTGGGA
  901 CAACTCCAGT GAAAAGTTCT TCTCCTTTAC TCATGATATC CGGATCCAGG CCTGTCGACG
  961 CTAGCTTTAA CAAAGCGACT ATAAGTTCAG AAAGTGAGAT GAGATTGGAA ATTAGTAATC
 1021 CAACCTAATT CAATTGATGG ATATGGCCAA GAATGTTCTA TCCTTGAATA TATCTTCGAT
1081 GTTTCCGATT AAACCA
//
Primer: ADH-Forward RDB14458_A6L7c.seq
>06188_14458_A6L7_ADH-Forward_C03_03_ABI08.ab1
    1 AGCGGCCCTT TAATGGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
   61 ACTAGTAAGA TGATTCTTTT ATCGTCGATA ACGATGAGGA TGTAGACGAT TATGATGAGA
  121 GTGATTAGCT TCTAAAATTT GGTGTTTTCA AACGCTATTT ACAGTACAAT CTGAAAAAAT
  181 TGGATGAACA TTGTAAATGG TAGGATACGA GTTCCAATTA ATGTATTTTT TAAGTATTAC
  241 ACTTAGCTTA TTAATTTTAT ATATACATTC TACCTCTGTG GGTTTAAAGA CAACTCGTAC
  301 ACATACTCAA ATTATTTATG AACATTATAA TGCATATTCG TACTGTCTAT TAAACATTTT
  361 TTTGACATTA AAAATTTCGT TTACTAACGT AGAAAGCGCT GATAATTGAG GCTACTATTT
  421 TAATATCTAG AAACAAAGAT TCATTAATTA ATTAAGTAAG CTCCAGGATA CTTGTATATT
  481 TCGTTAAAAA AGCGCAATTT CAACAATTCC TATGAACATC CATGATTTCA TCACAAAATC
  541 AAACATATCA GGATTTAACG TGAATTATTT GCTTTCTTGT GATTTTCATC TGATTTCTGG
  601 TCATTTACGT TACTGTAAAC AAGCTTTTTA AAAAAAGGAT TTTTTACGCA ATATTATCGA
  661 TATCCCAATC TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT
  721 TGCGGTAGGG TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG
  781 TGTGCAAAGA AAATCGTCGT CTTACCAGGA GACCATATTG GCCCTGAAAT TGTTGCTTCT
  841 GCCTTGGAGG TTTTGAAAGT CGTTGAGAAG AAGCGACCTG AGTTTAAAAC TCGAGTTTGA
  901 AGAACACAAG ATTGGAGGTG CCTCTATTGA TGCCTATGGG AACCCCTTTG ACTGACGAGA
  961 CTGTGAAAGC TTGTTTGGAA GCTGACGTGT TCTTTGGGTG CCGTTGGTGG TCTTGAATTG
 1021 ACGACCCCAT TGTCGTCTGA GCCAAGGTTA TGAGCTTCGT AGAGTATGAG ATTAGGACCA
1081 ACCATG
//
Primer: ars_R1 RDB14458_A6L7d.seq
>06188_14458_A6L7_ars_R1_D03_04_ABI08.ab1
    1 TGGTTCGGTT TATCTAATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT
   61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA
  121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT
  181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG
  241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA
  301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC
  361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG
  421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT
  481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC
  541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT
  601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA
  661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG
  721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT
  781 TGACGAAGAA ATCCGAAATC TTAGAATTGG TAGATAAAAT TGGACCCTAT GTCTGTGTTA
  841 TCAAGACACA TATTGACGTT GTCGAGGATT TCGACCAGGA TATGGTAGAA AAACTGGTGG
  901 CCTTAGTAAA AGCATCGTTT TTCTTATCTT GAGATCGCAA ATTTCGCAGA CATTTGGAAA
  961 TACCGGTCAA GCTACATATG CATCCTGGTG TGTACAAAAT GCTTCTTGGG CTTCATATCA
 1021 CAATGCATAA CAGTGACAAG GCGAGGGTAT TAATACCATG CTCAGAGTTG ATACCTTGGG
1081 GACCGGTG
//
Primer: f1ori_R3 RDB14458_A6L7e.seq
>06188_14488_A6L7_f1ori_R3_E03_05_ABI08.ab1
    1 GGGGTTGGGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
  241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
  301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
  361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
  421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
  481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCCAAGA CGAGAAGATG CCCCAACATT
  541 ATCTTTTTCT AGTAATGTGA CACGGAGCAC GAGAGGGGGT AAAATTTTCC TCTAAGCGGG
  601 CCGGGGGCCC AACCTTCCAA TTAATTAAAA TCGAAGTCCT CCCTCACAAT GATTTACAAA
  661 GTTGTTGCAT AAGAAATCCC TTAAGTAAGA GAAGATTGTT TAGTGGATTT TCTTTCACGT
  721 TTACTAAAAA TTTACATACA AAATGGATGG ATCTTCTTTC TCAATTGTTT CCCTTTCTTT
  781 CTTGCAAACG CGTTTAACAA AGAAGATGAC ATGCTATTTT CTGATAATTT TTTCTATAGT
  841 TTTCTAATTT TTGTACGTCC TTGGGGAGTC CGAATGTAAA ATTCGGGGTA TGGTAGTAGC
  901 GGCAGCCGTT CATTATGGTT ATTTAGGATA AAGTTAGGGC CAAGCGAGAA ACAGAATGGG
  961 ATAACAAGGC ATCGGTCTTA TAAATATTAG CTGAAAACTG CAATTATTGA ATTTTGATTA
1021 TTTCCATGGT ACTCAAATGA ATGCCGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl