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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-GFH1

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter

Catalog number RDB06188
Resource name pDUAL-GFH1
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364055.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06188 pDUAL-GFH1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06188z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14458_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14458_A6L7a.seq check
            >06188_14458_A6L7_nmt1Fw_A03_01_ABI08.ab1
                1 TAAATTTTAA TTAATCAGGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 ATATAAAGGA AGAGGAATCC TGGCATATCA TCAATTGAAT AAGTTGAATT AATTATTTCA
              241 ATCTCATTCT CACTTTCTGA CTTATAGTCG CTTTGTTAAA GCTAGCGTCG ACAGGCCTGG
              301 ATCCGGATAT CATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG
              361 AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG
              421 CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCGT
              481 GGCCAACACT TGTCACTACT TTCTGTTATG GTGTTCAATG CTTTTCAAGA TACCCAGATC
              541 ACATGAAACA GCATGACTTT TTCAAGAGTG CCATGCCCGA AGGCTATGTA CAGGAAAGAA
              601 CTATATTTTT CAAAGATGAC GGGAACTACA AGACACGTGC TGAAGTCAAG TTTGAAGGTG
              661 ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC
              721 TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC
              781 AAAAGAATGG GAATCAAAGT TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT
              841 CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA
              901 GACAACCATT ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC
              961 CACATGGGTC CTTTCTTGAG TTTGTAACAG CCTGCTGGGA TTACACATGG CATGGGATGG
             1021 ACTATACAAC CCATGGACTA CCAGGACGAC GATGAACTAA CCATCACACC ATCACCACTT
            1081 GGAGATCTCG ACTCCTAGAA GGAATTCCCG
            //
            Primer: ADH_rev
            Sequence file: RDB14458_A6L7b.seq check
            >06188_14458_A6L7_ADH_rev_B03_02_ABI08.ab1
                1 GGGGGGTTAA TCAAGGAATA AGAATTTTCG TTTTAAACCT AAGGAGGTCA CTTTAAAATT
               61 TGGTATACAC TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA
              121 TTCGCTTATT TAGAAGTGGC GCGCCGGATC CTCTAGAGTC GAGATCTCAG TGGTGATGGT
              181 GGTGATGTTT GTCATCGTCG TCCTTGTAGT CCATGGGTTT GTATAGTTCA TCCATGCCAT
              241 GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT GTGGTCTCTC TTTTCGTTGG
              301 GATCTTTCGA AAGGGCAGAT TGTGTGGACA GGTAATGGTT GTCTGGTAAA AGGACAGGGC
              361 CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGCTT CCATCTTCAA
              421 TGTTGTGTCT AATTTTGAAG TTAACTTTGA TTCCATTCTT TTGTTTGTCT GCCATGATGT
              481 ATACATTGTG TGAGTTATAG TTGTATTCCA ATTTGTGTCC AAGAATGTTT CCATCTTCTT
              541 TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT ATCACCTTCA AACTTGACTT
              601 CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT AGTTCTTTCC TGTACATAGC
              661 CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCTGTTTCAT GTGATCTGGG TATCTTGAAA
              721 AGCATTGAAC ACCATAACAG AAAGTAGTGA CAAGTGTTGG CCACGGAACA GGTAGTTTTC
              781 CAGTAGTGCA AATAAATTTA AGGGTAAGTT TTCCGTATGT TGCATCACCT TCACCCTCTC
              841 CACTGACAGA AAATTTTGTG CCCATTAACA TCACCATCTA AATTCAACAA GAATTTGGGA
              901 CAACTCCAGT GAAAAGTTCT TCTCCTTTAC TCATGATATC CGGATCCAGG CCTGTCGACG
              961 CTAGCTTTAA CAAAGCGACT ATAAGTTCAG AAAGTGAGAT GAGATTGGAA ATTAGTAATC
             1021 CAACCTAATT CAATTGATGG ATATGGCCAA GAATGTTCTA TCCTTGAATA TATCTTCGAT
            1081 GTTTCCGATT AAACCA
            //
            Primer: ADH-Forward
            Sequence file: RDB14458_A6L7c.seq check
            >06188_14458_A6L7_ADH-Forward_C03_03_ABI08.ab1
                1 AGCGGCCCTT TAATGGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
               61 ACTAGTAAGA TGATTCTTTT ATCGTCGATA ACGATGAGGA TGTAGACGAT TATGATGAGA
              121 GTGATTAGCT TCTAAAATTT GGTGTTTTCA AACGCTATTT ACAGTACAAT CTGAAAAAAT
              181 TGGATGAACA TTGTAAATGG TAGGATACGA GTTCCAATTA ATGTATTTTT TAAGTATTAC
              241 ACTTAGCTTA TTAATTTTAT ATATACATTC TACCTCTGTG GGTTTAAAGA CAACTCGTAC
              301 ACATACTCAA ATTATTTATG AACATTATAA TGCATATTCG TACTGTCTAT TAAACATTTT
              361 TTTGACATTA AAAATTTCGT TTACTAACGT AGAAAGCGCT GATAATTGAG GCTACTATTT
              421 TAATATCTAG AAACAAAGAT TCATTAATTA ATTAAGTAAG CTCCAGGATA CTTGTATATT
              481 TCGTTAAAAA AGCGCAATTT CAACAATTCC TATGAACATC CATGATTTCA TCACAAAATC
              541 AAACATATCA GGATTTAACG TGAATTATTT GCTTTCTTGT GATTTTCATC TGATTTCTGG
              601 TCATTTACGT TACTGTAAAC AAGCTTTTTA AAAAAAGGAT TTTTTACGCA ATATTATCGA
              661 TATCCCAATC TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT
              721 TGCGGTAGGG TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG
              781 TGTGCAAAGA AAATCGTCGT CTTACCAGGA GACCATATTG GCCCTGAAAT TGTTGCTTCT
              841 GCCTTGGAGG TTTTGAAAGT CGTTGAGAAG AAGCGACCTG AGTTTAAAAC TCGAGTTTGA
              901 AGAACACAAG ATTGGAGGTG CCTCTATTGA TGCCTATGGG AACCCCTTTG ACTGACGAGA
              961 CTGTGAAAGC TTGTTTGGAA GCTGACGTGT TCTTTGGGTG CCGTTGGTGG TCTTGAATTG
             1021 ACGACCCCAT TGTCGTCTGA GCCAAGGTTA TGAGCTTCGT AGAGTATGAG ATTAGGACCA
            1081 ACCATG
            //
            Primer: ars_R1
            Sequence file: RDB14458_A6L7d.seq check
            >06188_14458_A6L7_ars_R1_D03_04_ABI08.ab1
                1 TGGTTCGGTT TATCTAATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT
               61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA
              121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT
              181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG
              241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA
              301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC
              361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG
              421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT
              481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC
              541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT
              601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA
              661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG
              721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT
              781 TGACGAAGAA ATCCGAAATC TTAGAATTGG TAGATAAAAT TGGACCCTAT GTCTGTGTTA
              841 TCAAGACACA TATTGACGTT GTCGAGGATT TCGACCAGGA TATGGTAGAA AAACTGGTGG
              901 CCTTAGTAAA AGCATCGTTT TTCTTATCTT GAGATCGCAA ATTTCGCAGA CATTTGGAAA
              961 TACCGGTCAA GCTACATATG CATCCTGGTG TGTACAAAAT GCTTCTTGGG CTTCATATCA
             1021 CAATGCATAA CAGTGACAAG GCGAGGGTAT TAATACCATG CTCAGAGTTG ATACCTTGGG
            1081 GACCGGTG
            //
            Primer: f1ori_R3
            Sequence file: RDB14458_A6L7e.seq check
            >06188_14488_A6L7_f1ori_R3_E03_05_ABI08.ab1
                1 GGGGTTGGGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
              241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
              301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
              361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
              421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
              481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCCAAGA CGAGAAGATG CCCCAACATT
              541 ATCTTTTTCT AGTAATGTGA CACGGAGCAC GAGAGGGGGT AAAATTTTCC TCTAAGCGGG
              601 CCGGGGGCCC AACCTTCCAA TTAATTAAAA TCGAAGTCCT CCCTCACAAT GATTTACAAA
              661 GTTGTTGCAT AAGAAATCCC TTAAGTAAGA GAAGATTGTT TAGTGGATTT TCTTTCACGT
              721 TTACTAAAAA TTTACATACA AAATGGATGG ATCTTCTTTC TCAATTGTTT CCCTTTCTTT
              781 CTTGCAAACG CGTTTAACAA AGAAGATGAC ATGCTATTTT CTGATAATTT TTTCTATAGT
              841 TTTCTAATTT TTGTACGTCC TTGGGGAGTC CGAATGTAAA ATTCGGGGTA TGGTAGTAGC
              901 GGCAGCCGTT CATTATGGTT ATTTAGGATA AAGTTAGGGC CAAGCGAGAA ACAGAATGGG
              961 ATAACAAGGC ATCGGTCTTA TAAATATTAG CTGAAAACTG CAATTATTGA ATTTTGATTA
            1021 TTTCCATGGT ACTCAAATGA ATGCCGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl