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pDUAL-FFH81

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06187
Resource name pDUAL-FFH81
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364054.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06187 pDUAL-FFH81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06187z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14486_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14486_A6L7a.seq
>06187_14486_A6L7_nmt1Fw_8_B01_02_ABI08.ab1
    1 AGGGATATTA ATTAATCAAG GAAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
   61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
  121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
  181 ATGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC
  241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG
  301 GATATCATGG ATTATAAGGA TGATGACGAT AAACCCATGG ACTACAAGGA CGACGATGAC
  361 AAACATCACC ACCATCACCA CTGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA
  421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA
  481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA
  541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC
  601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTAA GATGATTCTT
  661 TTATCGTCGA TAACGATGAG GATGTAGACG ATTATGATGA GAGTGATTAG CTTCTAAAAT
  721 TTGGTGTTTT CAAACGCTAT TTACAGTACA ATCTGAAAAA ATTGGATGAA CATTGTAAAT
  781 GGTAGGGATA CGAGTTCCAA TTAATGTATT TTTTAAGTAT TACACTTAGC TTATTAATTT
  841 TATATATACA TTCTACCTCT GTGGGTTTAA AGACAACTCG TACACATACT CAAATTATTT
  901 ATGGAACATT ATAATGCATA TTCGTACTGT CTATTAAACA TTTTTTTTGA CATTAAAAAT
  961 TTCGTTTACT AAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT ATCTAGAACA
 1021 AAGGATTCAT TAATTTAATT TAAGGTAAGC TCCAGGGATA CTTGTATATT TTCGGTTTAG
 1081 AAAAAGCGGC AGTTTCACAA TTCCTATGAA CATTCATGAT TCAATCACAA AATTCAACAT
1141 ATCCAGGAAT TTTAACGGTG AT
//
Primer: LEU1pro_F RDB14486_A6L7b.seq
>06187_14486_A6L7_LEU1pro_F_8_D01_04_ABI08.ab1
    1 GATTAAAGTG CACAGTGTTG AGTTTCCCGA AACCAGGCAT TTTTGCGGTA GGGTCTGAAA
   61 CTTTCACGAA ACCATTTCAC AACAAAACTT CTTCAACAAC ATGTGTGCAA AGAAAATCGT
  121 CGTCTTACCA GGAGACCATA TTGGCCCTGA AATTGTTGCT TCTGCCTTGG AGGTTTTGAA
  181 AGTCGTTGAG AAGAAGCGAC CTGAGTTAAA ACTCGAGTTT GAAGAACACA AGATTGGAGG
  241 TGCCTCTATT GATGCCTATG GAACCCCTTT GACTGACGAG ACTGTGAAGG CTTGTTTGGA
  301 AGCTGACGGT GTTCTTTTGG GTGCCGTTGG TGGTCCTGAA TGGACCAACC CCAATTGTCG
  361 TCCTGAGCAA GGTTTATTGA AGCTTCGTAA GAGTATGGGT GTTTGGGCCA ACCTTCGACC
  421 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTGAAGCCTG AAATCGTTGA
  481 AGGTGTCGAT TTTTGTGTTG TACGAGAACT TACTGGAGGT TGTTACTTTG GTGAGCGCAC
  541 TGAGGACAAC GGATCGGGTT ATGCTATGGA CACTTGGCCT TACAGTTTGG AAGAAGTTTC
  601 TCGTATTGCT CGTTTGGCGG CCGCGGGCCC AACACCAATG TTTATAACCA AGTTTTATCT
  661 TGTTTGTCTA CATGGTATTT TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA
  721 GTTATCATTA CAAGTCTAAA AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA
  781 AAGATTGTGG TAATGTTGTA GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA
  841 TTCCCAAGGT GTTTATCTAT AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA
  901 GAGTACTTGT ATACTAATTC TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC
  961 AAACAAAGGC ATCGACTTTT TCAATAACCA ACCAAAAAAA TTTTACATTA GTCTTTTTTT
 1021 AATGCTGAGA AAGTCTTTGC TGATATGCCT TCCCAACCAG CTTCTTCTAT ATCCTTCTTG
 1081 GCTTTCGACA CAGATTAACG ACCCAGCTCC ATAGAACTCA CGAACCCAAC ATTGATTGAA
1141 TGTCGCTACG CAGTTACATC ACTCTCAGGA GTACCGATAA AATTGC
//
Primer: ars_R1 RDB14486_A6L7c.seq
>06187_14486_A6L7_ars_R1_8_F01_06_ABI08.ab1
    1 TTGGTTTCGG TTTATACTAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA
   61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT
  121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA
  181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA
  241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT
  301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA
  361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA
  421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG
  481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT
  541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG
  601 TATTGTCCTA CCAAGAACCT CTTTTTTGCT TGGATCGAAA TTAAAGGTTT AAAAGCAAAG
  661 TTATGGATGC TAGAGTATTT CAAAGCTATT CAGCTAGAGC TGAGGGGATG AAAAATCCCA
  721 TTGCCAAGGA ATTGTTGGCT TTGATGGAAG AAAAGCAAAG CAACTTGTCA GTCGCGGTCG
  781 ATTTGACGAA GAAATCCGAA ATCTTAGAAT TGGTAGATAA AATTGGACCC TATGTCTGTG
  841 TTATCAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCA GGATATGGTA GAAAAACTGG
  901 TGGCCTTAGG TAAAAAGCAT CGTTTTCTTA TCTTTGAGGA TCGCAAATTC GCAGACATTG
  961 GAAATACCGT CAAGCTACAT ATGCATCTGG GTGTGTAACA AATTTGCTTC TTGGCTCATA
 1021 TCACAATTTG CCATACAGTG CCAGGCGAAG GGTATTATAC AGCTCAAAGA AGTGGTTTAC
 1081 CCTTTGGACG TGGTCTCTTG CTTTTGGGCT GGATGTCTTC CAGGCTCTTT TGGCTACTGG
1141 TTTCCTTACC ACGGAGAAAA CCTTTAGA
//
Primer: f1ori_R3 RDB14486_A6L7d.seq
>06187_14486_A6L7_f1ori_R3_8_H01_08_ABI08.ab1
    1 GAAATGGAGG CTTGAGGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC
   61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
  121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
  181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG
  241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC
  301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA
  361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG
  421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA
  481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA
  541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG
  601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG
  661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA
  721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG
  781 AGATGACATG CTATTTTCTG ATTATTTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG
  841 GGAGTCCGAA ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA
  901 AAAGATAAAA AAGTAGGGGC AAGCGAGACA GAATGGGATA CAAGGGCATC GTCTATAATT
  961 ATAGCTAAAA ATTGTATTTT AATTTGTATT TTTTGTAATT TTATTTCTCA TGTTTACTAA
 1021 ATGAATGCGA ATTAGAGAAA CTTCAACGAA TGTCAAAATA GCTCAGCCAA ATTATACCAG
1081 TTTTAGAAGC GATCAGCACT ATCTCGATGT TAGACTTCCT TTTTCTTCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl