RDB06187 - pDUAL-FFH81

Resource data sheet
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pDUAL-FFH81

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter

Catalog number	RDB06187
Resource name	pDUAL-FFH81
Clone info.	S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364054.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06187	pDUAL-FFH81	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06187z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14486_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw	RDB14486_A6L7a.seq
>06187_14486_A6L7_nmt1Fw_8_B01_02_ABI08.ab1 1 AGGGATATTA ATTAATCAAG GAAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC 61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT 121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG 181 ATGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC 241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG 301 GATATCATGG ATTATAAGGA TGATGACGAT AAACCCATGG ACTACAAGGA CGACGATGAC 361 AAACATCACC ACCATCACCA CTGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA 421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA 481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA 541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC 601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTAA GATGATTCTT 661 TTATCGTCGA TAACGATGAG GATGTAGACG ATTATGATGA GAGTGATTAG CTTCTAAAAT 721 TTGGTGTTTT CAAACGCTAT TTACAGTACA ATCTGAAAAA ATTGGATGAA CATTGTAAAT 781 GGTAGGGATA CGAGTTCCAA TTAATGTATT TTTTAAGTAT TACACTTAGC TTATTAATTT 841 TATATATACA TTCTACCTCT GTGGGTTTAA AGACAACTCG TACACATACT CAAATTATTT 901 ATGGAACATT ATAATGCATA TTCGTACTGT CTATTAAACA TTTTTTTTGA CATTAAAAAT 961 TTCGTTTACT AAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT ATCTAGAACA 1021 AAGGATTCAT TAATTTAATT TAAGGTAAGC TCCAGGGATA CTTGTATATT TTCGGTTTAG 1081 AAAAAGCGGC AGTTTCACAA TTCCTATGAA CATTCATGAT TCAATCACAA AATTCAACAT 1141 ATCCAGGAAT TTTAACGGTG AT //
Primer: LEU1pro_F	RDB14486_A6L7b.seq
>06187_14486_A6L7_LEU1pro_F_8_D01_04_ABI08.ab1 1 GATTAAAGTG CACAGTGTTG AGTTTCCCGA AACCAGGCAT TTTTGCGGTA GGGTCTGAAA 61 CTTTCACGAA ACCATTTCAC AACAAAACTT CTTCAACAAC ATGTGTGCAA AGAAAATCGT 121 CGTCTTACCA GGAGACCATA TTGGCCCTGA AATTGTTGCT TCTGCCTTGG AGGTTTTGAA 181 AGTCGTTGAG AAGAAGCGAC CTGAGTTAAA ACTCGAGTTT GAAGAACACA AGATTGGAGG 241 TGCCTCTATT GATGCCTATG GAACCCCTTT GACTGACGAG ACTGTGAAGG CTTGTTTGGA 301 AGCTGACGGT GTTCTTTTGG GTGCCGTTGG TGGTCCTGAA TGGACCAACC CCAATTGTCG 361 TCCTGAGCAA GGTTTATTGA AGCTTCGTAA GAGTATGGGT GTTTGGGCCA ACCTTCGACC 421 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTGAAGCCTG AAATCGTTGA 481 AGGTGTCGAT TTTTGTGTTG TACGAGAACT TACTGGAGGT TGTTACTTTG GTGAGCGCAC 541 TGAGGACAAC GGATCGGGTT ATGCTATGGA CACTTGGCCT TACAGTTTGG AAGAAGTTTC 601 TCGTATTGCT CGTTTGGCGG CCGCGGGCCC AACACCAATG TTTATAACCA AGTTTTATCT 661 TGTTTGTCTA CATGGTATTT TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA 721 GTTATCATTA CAAGTCTAAA AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA 781 AAGATTGTGG TAATGTTGTA GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA 841 TTCCCAAGGT GTTTATCTAT AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA 901 GAGTACTTGT ATACTAATTC TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC 961 AAACAAAGGC ATCGACTTTT TCAATAACCA ACCAAAAAAA TTTTACATTA GTCTTTTTTT 1021 AATGCTGAGA AAGTCTTTGC TGATATGCCT TCCCAACCAG CTTCTTCTAT ATCCTTCTTG 1081 GCTTTCGACA CAGATTAACG ACCCAGCTCC ATAGAACTCA CGAACCCAAC ATTGATTGAA 1141 TGTCGCTACG CAGTTACATC ACTCTCAGGA GTACCGATAA AATTGC //
Primer: ars_R1	RDB14486_A6L7c.seq
>06187_14486_A6L7_ars_R1_8_F01_06_ABI08.ab1 1 TTGGTTTCGG TTTATACTAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA 61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT 121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA 181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA 241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT 301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA 361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA 421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG 481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT 541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG 601 TATTGTCCTA CCAAGAACCT CTTTTTTGCT TGGATCGAAA TTAAAGGTTT AAAAGCAAAG 661 TTATGGATGC TAGAGTATTT CAAAGCTATT CAGCTAGAGC TGAGGGGATG AAAAATCCCA 721 TTGCCAAGGA ATTGTTGGCT TTGATGGAAG AAAAGCAAAG CAACTTGTCA GTCGCGGTCG 781 ATTTGACGAA GAAATCCGAA ATCTTAGAAT TGGTAGATAA AATTGGACCC TATGTCTGTG 841 TTATCAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCA GGATATGGTA GAAAAACTGG 901 TGGCCTTAGG TAAAAAGCAT CGTTTTCTTA TCTTTGAGGA TCGCAAATTC GCAGACATTG 961 GAAATACCGT CAAGCTACAT ATGCATCTGG GTGTGTAACA AATTTGCTTC TTGGCTCATA 1021 TCACAATTTG CCATACAGTG CCAGGCGAAG GGTATTATAC AGCTCAAAGA AGTGGTTTAC 1081 CCTTTGGACG TGGTCTCTTG CTTTTGGGCT GGATGTCTTC CAGGCTCTTT TGGCTACTGG 1141 TTTCCTTACC ACGGAGAAAA CCTTTAGA //
Primer: f1ori_R3	RDB14486_A6L7d.seq
>06187_14486_A6L7_f1ori_R3_8_H01_08_ABI08.ab1 1 GAAATGGAGG CTTGAGGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC 61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG 121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG 181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG 241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC 301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA 361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG 421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA 481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA 541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG 601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG 661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA 721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG 781 AGATGACATG CTATTTTCTG ATTATTTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG 841 GGAGTCCGAA ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA 901 AAAGATAAAA AAGTAGGGGC AAGCGAGACA GAATGGGATA CAAGGGCATC GTCTATAATT 961 ATAGCTAAAA ATTGTATTTT AATTTGTATT TTTTGTAATT TTATTTCTCA TGTTTACTAA 1021 ATGAATGCGA ATTAGAGAAA CTTCAACGAA TGTCAAAATA GCTCAGCCAA ATTATACCAG 1081 TTTTAGAAGC GATCAGCACT ATCTCGATGT TAGACTTCCT TTTTCTTCA //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl