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pDUAL-FFH81 (#RDB06187)

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter


Drawn by SnapGene® software
Sequence (full) RDB06187hts01.seq
Publication Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012). [link to RRC of NBRP]
Publication Matsuyama, A., Yeast,.21 (15):1289-1305 (2004). [link to RRC of NBRP]
Test sheet RDB20206_B3Khp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Reference of insert sequence AB364054.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

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RDB06187 pDUAL-FFH81 DNA solution

How to cite this biological resource

Materials & Methods section:

The pDUAL-FFH81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06187).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]