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pDUAL-FFH81

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06187
Resource name pDUAL-FFH81
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364054.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06187 pDUAL-FFH81 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06187z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14486_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14486_A6L7a.seq check
            >06187_14486_A6L7_nmt1Fw_8_B01_02_ABI08.ab1
                1 AGGGATATTA ATTAATCAAG GAAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
               61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
              121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
              181 ATGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC
              241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG
              301 GATATCATGG ATTATAAGGA TGATGACGAT AAACCCATGG ACTACAAGGA CGACGATGAC
              361 AAACATCACC ACCATCACCA CTGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA
              421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA
              481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA
              541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC
              601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTAA GATGATTCTT
              661 TTATCGTCGA TAACGATGAG GATGTAGACG ATTATGATGA GAGTGATTAG CTTCTAAAAT
              721 TTGGTGTTTT CAAACGCTAT TTACAGTACA ATCTGAAAAA ATTGGATGAA CATTGTAAAT
              781 GGTAGGGATA CGAGTTCCAA TTAATGTATT TTTTAAGTAT TACACTTAGC TTATTAATTT
              841 TATATATACA TTCTACCTCT GTGGGTTTAA AGACAACTCG TACACATACT CAAATTATTT
              901 ATGGAACATT ATAATGCATA TTCGTACTGT CTATTAAACA TTTTTTTTGA CATTAAAAAT
              961 TTCGTTTACT AAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT ATCTAGAACA
             1021 AAGGATTCAT TAATTTAATT TAAGGTAAGC TCCAGGGATA CTTGTATATT TTCGGTTTAG
             1081 AAAAAGCGGC AGTTTCACAA TTCCTATGAA CATTCATGAT TCAATCACAA AATTCAACAT
            1141 ATCCAGGAAT TTTAACGGTG AT
            //
            Primer: LEU1pro_F
            Sequence file: RDB14486_A6L7b.seq check
            >06187_14486_A6L7_LEU1pro_F_8_D01_04_ABI08.ab1
                1 GATTAAAGTG CACAGTGTTG AGTTTCCCGA AACCAGGCAT TTTTGCGGTA GGGTCTGAAA
               61 CTTTCACGAA ACCATTTCAC AACAAAACTT CTTCAACAAC ATGTGTGCAA AGAAAATCGT
              121 CGTCTTACCA GGAGACCATA TTGGCCCTGA AATTGTTGCT TCTGCCTTGG AGGTTTTGAA
              181 AGTCGTTGAG AAGAAGCGAC CTGAGTTAAA ACTCGAGTTT GAAGAACACA AGATTGGAGG
              241 TGCCTCTATT GATGCCTATG GAACCCCTTT GACTGACGAG ACTGTGAAGG CTTGTTTGGA
              301 AGCTGACGGT GTTCTTTTGG GTGCCGTTGG TGGTCCTGAA TGGACCAACC CCAATTGTCG
              361 TCCTGAGCAA GGTTTATTGA AGCTTCGTAA GAGTATGGGT GTTTGGGCCA ACCTTCGACC
              421 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTGAAGCCTG AAATCGTTGA
              481 AGGTGTCGAT TTTTGTGTTG TACGAGAACT TACTGGAGGT TGTTACTTTG GTGAGCGCAC
              541 TGAGGACAAC GGATCGGGTT ATGCTATGGA CACTTGGCCT TACAGTTTGG AAGAAGTTTC
              601 TCGTATTGCT CGTTTGGCGG CCGCGGGCCC AACACCAATG TTTATAACCA AGTTTTATCT
              661 TGTTTGTCTA CATGGTATTT TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA
              721 GTTATCATTA CAAGTCTAAA AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA
              781 AAGATTGTGG TAATGTTGTA GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA
              841 TTCCCAAGGT GTTTATCTAT AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA
              901 GAGTACTTGT ATACTAATTC TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC
              961 AAACAAAGGC ATCGACTTTT TCAATAACCA ACCAAAAAAA TTTTACATTA GTCTTTTTTT
             1021 AATGCTGAGA AAGTCTTTGC TGATATGCCT TCCCAACCAG CTTCTTCTAT ATCCTTCTTG
             1081 GCTTTCGACA CAGATTAACG ACCCAGCTCC ATAGAACTCA CGAACCCAAC ATTGATTGAA
            1141 TGTCGCTACG CAGTTACATC ACTCTCAGGA GTACCGATAA AATTGC
            //
            Primer: ars_R1
            Sequence file: RDB14486_A6L7c.seq check
            >06187_14486_A6L7_ars_R1_8_F01_06_ABI08.ab1
                1 TTGGTTTCGG TTTATACTAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA
               61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT
              121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA
              181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA
              241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT
              301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA
              361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA
              421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG
              481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT
              541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG
              601 TATTGTCCTA CCAAGAACCT CTTTTTTGCT TGGATCGAAA TTAAAGGTTT AAAAGCAAAG
              661 TTATGGATGC TAGAGTATTT CAAAGCTATT CAGCTAGAGC TGAGGGGATG AAAAATCCCA
              721 TTGCCAAGGA ATTGTTGGCT TTGATGGAAG AAAAGCAAAG CAACTTGTCA GTCGCGGTCG
              781 ATTTGACGAA GAAATCCGAA ATCTTAGAAT TGGTAGATAA AATTGGACCC TATGTCTGTG
              841 TTATCAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCA GGATATGGTA GAAAAACTGG
              901 TGGCCTTAGG TAAAAAGCAT CGTTTTCTTA TCTTTGAGGA TCGCAAATTC GCAGACATTG
              961 GAAATACCGT CAAGCTACAT ATGCATCTGG GTGTGTAACA AATTTGCTTC TTGGCTCATA
             1021 TCACAATTTG CCATACAGTG CCAGGCGAAG GGTATTATAC AGCTCAAAGA AGTGGTTTAC
             1081 CCTTTGGACG TGGTCTCTTG CTTTTGGGCT GGATGTCTTC CAGGCTCTTT TGGCTACTGG
            1141 TTTCCTTACC ACGGAGAAAA CCTTTAGA
            //
            Primer: f1ori_R3
            Sequence file: RDB14486_A6L7d.seq check
            >06187_14486_A6L7_f1ori_R3_8_H01_08_ABI08.ab1
                1 GAAATGGAGG CTTGAGGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC
               61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
              121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
              181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG
              241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC
              301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA
              361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG
              421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA
              481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA
              541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG
              601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG
              661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA
              721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG
              781 AGATGACATG CTATTTTCTG ATTATTTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG
              841 GGAGTCCGAA ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA
              901 AAAGATAAAA AAGTAGGGGC AAGCGAGACA GAATGGGATA CAAGGGCATC GTCTATAATT
              961 ATAGCTAAAA ATTGTATTTT AATTTGTATT TTTTGTAATT TTATTTCTCA TGTTTACTAA
             1021 ATGAATGCGA ATTAGAGAAA CTTCAACGAA TGTCAAAATA GCTCAGCCAA ATTATACCAG
            1081 TTTTAGAAGC GATCAGCACT ATCTCGATGT TAGACTTCCT TTTTCTTCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl