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pDUAL-FFH41

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06186
Resource name pDUAL-FFH41
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364053.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06186 pDUAL-FFH41 DNA solution

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References and tips

Electronic file

Electronic file Sequence RDB06186z.seq from Depositor

Original reference

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14485_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw | Sequence: RDB14485_A6L7a.seq
>06186_14485_A6L7_nmt1Fw_D02_04_ABI08.ab1
    1 TAAAATTTAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
   61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
  121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
  181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC
  241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG
  301 GATATCATGG ATTATAAGGA TGATGACGAT AAACCCATGG ACTACAAGGA CGACGATGAC
  361 AAACATCACC ACCATCACCA CTGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA
  421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA
  481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA
  541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC
  601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTAA GATGATTCTT
  661 TTATCGTCGA TAACGATGAG GATGTAGACG ATTATGATGA GAGTGATTAG CTTCTAAAAT
  721 TTGGTGTTTT CAAACGCTAT TTACAGTACA ATCTGAAAAA ATTGGATGAA CATTGTAAAT
  781 GGTAGGATAC GAGTTCCAAT TAATGTATTT TTTTAAGTAT TACACTTAGC TTATTAATTT
  841 TATATATACA TTCTACCTCT GTGGGTTTAA AGACAACTCG TACACATACT CAAATTATTT
  901 ATGAACATTA TAATGCATAT TCGTACTGTC TATTAAACAT TTTTTTTTGA CATTAAAAAT
  961 TTCGTTTACT ACGTAGAAGC GCTGATTATT GAGCTACTAT TTTTATATCT AGAACCAAGG
 1021 ATCAATAAGT AATTAGTTTA GCTTCAGGGA TACCTGGATA TCGTAAAGCG CAGTTCCACA
1081 ACTCCTTATG CA
//
Primer: LEU1pro_F | Sequence: RDB14485_A6L7b.seq
>06186_14485_A6L7_LEU1pro_F_H06_24_ABI24.ab1
    1 CGATAAGGTG CAACAGTGTT GAGTTTCCCG AAACCAGGCA TTTTTGCGGT AGGGTCTGAA
   61 ACTTTCACGA AACCATTTCA CAACAAAACT TCTTCAACAA CATGTGTGCA AAGAAAATCG
  121 TCGTCTTACC AGGAGACCAT ATTGGCCCTG AAATTGTTGC TTCTGCCTTG GAGGTTTTGA
  181 AAGTCGTTGA GAAGAAGCGA CCTGAGTTAA AACTCGAGTT TGAAGAACAC AAGATTGGAG
  241 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG
  301 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC
  361 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC
  421 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG
  481 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA
  541 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT
  601 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACACCAAT GTTTATAACC AAGTTTTATC
  661 TTGTTTGTCT ACATGGTATT TTACATTCAT CTACATACAT CTTTTCATTG GCTTTGTACA
  721 TAGTTATCAT AACAAGTCTA AAGAATTCCT CCTTTTCTTA TTTCAATGTC AATCCCAAAA
781 GAAAAGAATT GTGGGTATTG TTGTAGGAGT CATGGTTAAA AGAAATCAGC ATG
//
Primer: ars_R1 | Sequence: RDB14485_A6L7c.seq
>06186_14485_A6L7_ars_R1_E02_05_ABI08.ab1
    1 TGGTTCGGTT TTATACTAAT TACTTAAGTA CCTTTAACTA AACAAAATGC CTATATATAT
   61 ATTAATTTAC AATGAGTGTC AGATAAGTCA CTATGTCCGA GTGGTTAAGG AGTTAGACTC
  121 GAATTCGCAA GCTTAGCTAC AAATCCCACT GGCTATATGT ATGCATTTGT GTTAAAAAAG
  181 TTTGTATAGA TTATTTAATC TACTCAGCAT TCTTTCTCTA AATAGGAATT TGTTACTTAA
  241 TGGAGAAAAA AATGTTTCGA TTTACCTAGT GTATTTGTTT GTATACTCAC GTTTAATTTC
  301 AAACATCCAT TCTATCTTGT GTAATTTTTG GCATGGTGAA AAAGATAATC AGCCTTATAA
  361 TCTTTACAAA AGTAAGAAAT TCTGTAAATA AGCCTTAATG CCCTTGCTTT AAATTAAAAT
  421 GGTTCTTTTT CATGATAATG TTTGCACTTT GTGAATATAT TTTAGATAGT TCTGTGAGGT
  481 ATAATTAAGA TGTTTTAGAG ACTTATACAA TTTTGTCTTT ATAAATTCTT AATTGATTTT
  541 ACCATCCCAG TTTAACTATG CTTCGTCGGC ATCTCTGCAC ATGTCGTGTT TTCTTACCGT
  601 ATTGTCCTAC CAAGAACCTC TTTTTTGCTT GGATCGAAAT TAAAGGTTTA AAAGCAAAGT
  661 TATGGATGCT AGAGTATTTC AAAGCTATTC AGCTAGAGCT GAGGGGATGA AAAATCCCAT
  721 TGCCAAGGAA TTGTTGGCTT TGATGGAAGA AAAGCAAAGC AACTTGTCAG TCGCGGTCGA
  781 TTTGACGAAG AAATCCGAAA TCTTAGAATT GGTAGATAAA ATTGGACCCT ATGTCTGTGT
  841 TATCAAGACA CATATTGACG TTGTCGAGGA TTTCGACCAG GATATGGTAG AAAAAACTGG
  901 TGGCCCTTAG TAAAAAGCAT CGTTTTCTTA TCTTTGAGAT CGCAAATTCG CAGACATTGG
  961 AAATACCGTC AGCTACAATA TGCATCTGGT GTGTACCAAA ATTGCTTTCC TTGGCTCATA
 1021 ATCACAAATT GGCCATAACA GTGCAAGGCC GAAGGTATTA ATACAGGCTT CAAGAAGTGA
1081 ATTAACGTTG GGACCG
//
Primer: f1ori_R3 | Sequence: RDB14485_A6L7d.seq
>06186_14485_A6L7_f1ori_R3_F02_06_ABI08.ab1
    1 GCCATGCGCT TTGAAGTATG GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC
   61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
  121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
  181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG
  241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC
  301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA
  361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG
  421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA
  481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA
  541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG
  601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG
  661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA
  721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG
  781 AGATGACATG CTATTTTCTG ATTATTTTTT TTCTATAGTT TTTCTATTTT TGTACGTCCT
  841 TGGGAGTCCG AAATGTAAAA TCGGGGTATG GGTTAGTAGC GGCAGCGTTC ATTATGGTAT
  901 ATAAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAAATGG GATACAAGGG CATCGTCTAA
  961 TAATTATAGC TAAAAATTGT AATTTTAAAT TTGTATTTTT TTGTAAATTT TTATTTCTCA
 1021 TGGTTTACTA AATGGAATGC GATTAGAGAA ACCTTCCACG AAATGTCAAT TAGCTCAGCC
1081 GAAGTATATA CGAA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report Fleck, O., Deoxynucleoside Salvage in Fission Yeast Allows Rescue of Ribonucleotide Reductase Deficiency but Not Spd1-Mediated Inhibition of Replication. Genes (Basel). 8 (5). pii: E128 (2017). PMID 28441348.

2019.10.05

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