RDB06186 - pDUAL-FFH41

Resource data sheet
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pDUAL-FFH41

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number	RDB06186
Resource name	pDUAL-FFH41
Clone info.	S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364053.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06186	pDUAL-FFH41	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06186z.seq from Depositor

Original reference

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14485_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw \| Sequence: RDB14485_A6L7a.seq
>06186_14485_A6L7_nmt1Fw_D02_04_ABI08.ab1 1 TAAAATTTAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC 61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG 121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA 181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC 241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG 301 GATATCATGG ATTATAAGGA TGATGACGAT AAACCCATGG ACTACAAGGA CGACGATGAC 361 AAACATCACC ACCATCACCA CTGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA 421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA 481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA 541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC 601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTAA GATGATTCTT 661 TTATCGTCGA TAACGATGAG GATGTAGACG ATTATGATGA GAGTGATTAG CTTCTAAAAT 721 TTGGTGTTTT CAAACGCTAT TTACAGTACA ATCTGAAAAA ATTGGATGAA CATTGTAAAT 781 GGTAGGATAC GAGTTCCAAT TAATGTATTT TTTTAAGTAT TACACTTAGC TTATTAATTT 841 TATATATACA TTCTACCTCT GTGGGTTTAA AGACAACTCG TACACATACT CAAATTATTT 901 ATGAACATTA TAATGCATAT TCGTACTGTC TATTAAACAT TTTTTTTTGA CATTAAAAAT 961 TTCGTTTACT ACGTAGAAGC GCTGATTATT GAGCTACTAT TTTTATATCT AGAACCAAGG 1021 ATCAATAAGT AATTAGTTTA GCTTCAGGGA TACCTGGATA TCGTAAAGCG CAGTTCCACA 1081 ACTCCTTATG CA //
Primer: LEU1pro_F \| Sequence: RDB14485_A6L7b.seq
>06186_14485_A6L7_LEU1pro_F_H06_24_ABI24.ab1 1 CGATAAGGTG CAACAGTGTT GAGTTTCCCG AAACCAGGCA TTTTTGCGGT AGGGTCTGAA 61 ACTTTCACGA AACCATTTCA CAACAAAACT TCTTCAACAA CATGTGTGCA AAGAAAATCG 121 TCGTCTTACC AGGAGACCAT ATTGGCCCTG AAATTGTTGC TTCTGCCTTG GAGGTTTTGA 181 AAGTCGTTGA GAAGAAGCGA CCTGAGTTAA AACTCGAGTT TGAAGAACAC AAGATTGGAG 241 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG 301 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC 361 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC 421 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG 481 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA 541 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT 601 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACACCAAT GTTTATAACC AAGTTTTATC 661 TTGTTTGTCT ACATGGTATT TTACATTCAT CTACATACAT CTTTTCATTG GCTTTGTACA 721 TAGTTATCAT AACAAGTCTA AAGAATTCCT CCTTTTCTTA TTTCAATGTC AATCCCAAAA 781 GAAAAGAATT GTGGGTATTG TTGTAGGAGT CATGGTTAAA AGAAATCAGC ATG //
Primer: ars_R1 \| Sequence: RDB14485_A6L7c.seq
>06186_14485_A6L7_ars_R1_E02_05_ABI08.ab1 1 TGGTTCGGTT TTATACTAAT TACTTAAGTA CCTTTAACTA AACAAAATGC CTATATATAT 61 ATTAATTTAC AATGAGTGTC AGATAAGTCA CTATGTCCGA GTGGTTAAGG AGTTAGACTC 121 GAATTCGCAA GCTTAGCTAC AAATCCCACT GGCTATATGT ATGCATTTGT GTTAAAAAAG 181 TTTGTATAGA TTATTTAATC TACTCAGCAT TCTTTCTCTA AATAGGAATT TGTTACTTAA 241 TGGAGAAAAA AATGTTTCGA TTTACCTAGT GTATTTGTTT GTATACTCAC GTTTAATTTC 301 AAACATCCAT TCTATCTTGT GTAATTTTTG GCATGGTGAA AAAGATAATC AGCCTTATAA 361 TCTTTACAAA AGTAAGAAAT TCTGTAAATA AGCCTTAATG CCCTTGCTTT AAATTAAAAT 421 GGTTCTTTTT CATGATAATG TTTGCACTTT GTGAATATAT TTTAGATAGT TCTGTGAGGT 481 ATAATTAAGA TGTTTTAGAG ACTTATACAA TTTTGTCTTT ATAAATTCTT AATTGATTTT 541 ACCATCCCAG TTTAACTATG CTTCGTCGGC ATCTCTGCAC ATGTCGTGTT TTCTTACCGT 601 ATTGTCCTAC CAAGAACCTC TTTTTTGCTT GGATCGAAAT TAAAGGTTTA AAAGCAAAGT 661 TATGGATGCT AGAGTATTTC AAAGCTATTC AGCTAGAGCT GAGGGGATGA AAAATCCCAT 721 TGCCAAGGAA TTGTTGGCTT TGATGGAAGA AAAGCAAAGC AACTTGTCAG TCGCGGTCGA 781 TTTGACGAAG AAATCCGAAA TCTTAGAATT GGTAGATAAA ATTGGACCCT ATGTCTGTGT 841 TATCAAGACA CATATTGACG TTGTCGAGGA TTTCGACCAG GATATGGTAG AAAAAACTGG 901 TGGCCCTTAG TAAAAAGCAT CGTTTTCTTA TCTTTGAGAT CGCAAATTCG CAGACATTGG 961 AAATACCGTC AGCTACAATA TGCATCTGGT GTGTACCAAA ATTGCTTTCC TTGGCTCATA 1021 ATCACAAATT GGCCATAACA GTGCAAGGCC GAAGGTATTA ATACAGGCTT CAAGAAGTGA 1081 ATTAACGTTG GGACCG //
Primer: f1ori_R3 \| Sequence: RDB14485_A6L7d.seq
>06186_14485_A6L7_f1ori_R3_F02_06_ABI08.ab1 1 GCCATGCGCT TTGAAGTATG GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC 61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG 121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG 181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG 241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC 301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA 361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG 421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA 481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA 541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG 601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG 661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA 721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG 781 AGATGACATG CTATTTTCTG ATTATTTTTT TTCTATAGTT TTTCTATTTT TGTACGTCCT 841 TGGGAGTCCG AAATGTAAAA TCGGGGTATG GGTTAGTAGC GGCAGCGTTC ATTATGGTAT 901 ATAAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAAATGG GATACAAGGG CATCGTCTAA 961 TAATTATAGC TAAAAATTGT AATTTTAAAT TTGTATTTTT TTGTAAATTT TTATTTCTCA 1021 TGGTTTACTA AATGGAATGC GATTAGAGAA ACCTTCCACG AAATGTCAAT TAGCTCAGCC 1081 GAAGTATATA CGAA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report	Fleck, O., Deoxynucleoside Salvage in Fission Yeast Allows Rescue of Ribonucleotide Reductase Deficiency but Not Spd1-Mediated Inhibition of Replication. Genes (Basel). 8 (5). pii: E128 (2017). PMID 28441348.

2019.10.05

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