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pDUAL-FFH41

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06186
Resource name pDUAL-FFH41
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364053.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06186 pDUAL-FFH41 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06186z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14485_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14485_A6L7a.seq check
            >06186_14485_A6L7_nmt1Fw_D02_04_ABI08.ab1
                1 TAAAATTTAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
               61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
              121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
              181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC
              241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG
              301 GATATCATGG ATTATAAGGA TGATGACGAT AAACCCATGG ACTACAAGGA CGACGATGAC
              361 AAACATCACC ACCATCACCA CTGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA
              421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA
              481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA
              541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC
              601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTAA GATGATTCTT
              661 TTATCGTCGA TAACGATGAG GATGTAGACG ATTATGATGA GAGTGATTAG CTTCTAAAAT
              721 TTGGTGTTTT CAAACGCTAT TTACAGTACA ATCTGAAAAA ATTGGATGAA CATTGTAAAT
              781 GGTAGGATAC GAGTTCCAAT TAATGTATTT TTTTAAGTAT TACACTTAGC TTATTAATTT
              841 TATATATACA TTCTACCTCT GTGGGTTTAA AGACAACTCG TACACATACT CAAATTATTT
              901 ATGAACATTA TAATGCATAT TCGTACTGTC TATTAAACAT TTTTTTTTGA CATTAAAAAT
              961 TTCGTTTACT ACGTAGAAGC GCTGATTATT GAGCTACTAT TTTTATATCT AGAACCAAGG
             1021 ATCAATAAGT AATTAGTTTA GCTTCAGGGA TACCTGGATA TCGTAAAGCG CAGTTCCACA
            1081 ACTCCTTATG CA
            //
            Primer: LEU1pro_F
            Sequence file: RDB14485_A6L7b.seq check
            >06186_14485_A6L7_LEU1pro_F_H06_24_ABI24.ab1
                1 CGATAAGGTG CAACAGTGTT GAGTTTCCCG AAACCAGGCA TTTTTGCGGT AGGGTCTGAA
               61 ACTTTCACGA AACCATTTCA CAACAAAACT TCTTCAACAA CATGTGTGCA AAGAAAATCG
              121 TCGTCTTACC AGGAGACCAT ATTGGCCCTG AAATTGTTGC TTCTGCCTTG GAGGTTTTGA
              181 AAGTCGTTGA GAAGAAGCGA CCTGAGTTAA AACTCGAGTT TGAAGAACAC AAGATTGGAG
              241 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG
              301 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC
              361 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC
              421 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG
              481 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA
              541 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT
              601 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACACCAAT GTTTATAACC AAGTTTTATC
              661 TTGTTTGTCT ACATGGTATT TTACATTCAT CTACATACAT CTTTTCATTG GCTTTGTACA
              721 TAGTTATCAT AACAAGTCTA AAGAATTCCT CCTTTTCTTA TTTCAATGTC AATCCCAAAA
            781 GAAAAGAATT GTGGGTATTG TTGTAGGAGT CATGGTTAAA AGAAATCAGC ATG
            //
            Primer: ars_R1
            Sequence file: RDB14485_A6L7c.seq check
            >06186_14485_A6L7_ars_R1_E02_05_ABI08.ab1
                1 TGGTTCGGTT TTATACTAAT TACTTAAGTA CCTTTAACTA AACAAAATGC CTATATATAT
               61 ATTAATTTAC AATGAGTGTC AGATAAGTCA CTATGTCCGA GTGGTTAAGG AGTTAGACTC
              121 GAATTCGCAA GCTTAGCTAC AAATCCCACT GGCTATATGT ATGCATTTGT GTTAAAAAAG
              181 TTTGTATAGA TTATTTAATC TACTCAGCAT TCTTTCTCTA AATAGGAATT TGTTACTTAA
              241 TGGAGAAAAA AATGTTTCGA TTTACCTAGT GTATTTGTTT GTATACTCAC GTTTAATTTC
              301 AAACATCCAT TCTATCTTGT GTAATTTTTG GCATGGTGAA AAAGATAATC AGCCTTATAA
              361 TCTTTACAAA AGTAAGAAAT TCTGTAAATA AGCCTTAATG CCCTTGCTTT AAATTAAAAT
              421 GGTTCTTTTT CATGATAATG TTTGCACTTT GTGAATATAT TTTAGATAGT TCTGTGAGGT
              481 ATAATTAAGA TGTTTTAGAG ACTTATACAA TTTTGTCTTT ATAAATTCTT AATTGATTTT
              541 ACCATCCCAG TTTAACTATG CTTCGTCGGC ATCTCTGCAC ATGTCGTGTT TTCTTACCGT
              601 ATTGTCCTAC CAAGAACCTC TTTTTTGCTT GGATCGAAAT TAAAGGTTTA AAAGCAAAGT
              661 TATGGATGCT AGAGTATTTC AAAGCTATTC AGCTAGAGCT GAGGGGATGA AAAATCCCAT
              721 TGCCAAGGAA TTGTTGGCTT TGATGGAAGA AAAGCAAAGC AACTTGTCAG TCGCGGTCGA
              781 TTTGACGAAG AAATCCGAAA TCTTAGAATT GGTAGATAAA ATTGGACCCT ATGTCTGTGT
              841 TATCAAGACA CATATTGACG TTGTCGAGGA TTTCGACCAG GATATGGTAG AAAAAACTGG
              901 TGGCCCTTAG TAAAAAGCAT CGTTTTCTTA TCTTTGAGAT CGCAAATTCG CAGACATTGG
              961 AAATACCGTC AGCTACAATA TGCATCTGGT GTGTACCAAA ATTGCTTTCC TTGGCTCATA
             1021 ATCACAAATT GGCCATAACA GTGCAAGGCC GAAGGTATTA ATACAGGCTT CAAGAAGTGA
            1081 ATTAACGTTG GGACCG
            //
            Primer: f1ori_R3
            Sequence file: RDB14485_A6L7d.seq check
            >06186_14485_A6L7_f1ori_R3_F02_06_ABI08.ab1
                1 GCCATGCGCT TTGAAGTATG GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC
               61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
              121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
              181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG
              241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC
              301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA
              361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG
              421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA
              481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA
              541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG
              601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG
              661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA
              721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG
              781 AGATGACATG CTATTTTCTG ATTATTTTTT TTCTATAGTT TTTCTATTTT TGTACGTCCT
              841 TGGGAGTCCG AAATGTAAAA TCGGGGTATG GGTTAGTAGC GGCAGCGTTC ATTATGGTAT
              901 ATAAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAAATGG GATACAAGGG CATCGTCTAA
              961 TAATTATAGC TAAAAATTGT AATTTTAAAT TTGTATTTTT TTGTAAATTT TTATTTCTCA
             1021 TGGTTTACTA AATGGAATGC GATTAGAGAA ACCTTCCACG AAATGTCAAT TAGCTCAGCC
            1081 GAAGTATATA CGAA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            user_report Fleck, O., Deoxynucleoside Salvage in Fission Yeast Allows Rescue of Ribonucleotide Reductase Deficiency but Not Spd1-Mediated Inhibition of Replication. Genes (Basel). 8 (5). pii: E128 (2017). PMID 28441348.

            2022.05.18

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