Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-FFH41

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14485_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14485_A6L7a.seq
>06186_14485_A6L7_nmt1Fw_D02_04_ABI08.ab1     1 TAAAATTTAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC    61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG   121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA   181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC   241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG   301 GATATCATGG ATTATAAGGA TGATGACGAT AAACCCATGG ACTACAAGGA CGACGATGAC   361 AAACATCACC ACCATCACCA CTGAGATCTC GACTCTAGAG GATCCGGCGC GCCACTTCTA   421 AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA   481 GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA   541 ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT CTTATTGACC   601 ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTAA GATGATTCTT   661 TTATCGTCGA TAACGATGAG GATGTAGACG ATTATGATGA GAGTGATTAG CTTCTAAAAT   721 TTGGTGTTTT CAAACGCTAT TTACAGTACA ATCTGAAAAA ATTGGATGAA CATTGTAAAT   781 GGTAGGATAC GAGTTCCAAT TAATGTATTT TTTTAAGTAT TACACTTAGC TTATTAATTT   841 TATATATACA TTCTACCTCT GTGGGTTTAA AGACAACTCG TACACATACT CAAATTATTT   901 ATGAACATTA TAATGCATAT TCGTACTGTC TATTAAACAT TTTTTTTTGA CATTAAAAAT   961 TTCGTTTACT ACGTAGAAGC GCTGATTATT GAGCTACTAT TTTTATATCT AGAACCAAGG  1021 ATCAATAAGT AATTAGTTTA GCTTCAGGGA TACCTGGATA TCGTAAAGCG CAGTTCCACA 1081 ACTCCTTATG CA //
Primer: LEU1pro_F Sequence file: RDB14485_A6L7b.seq
>06186_14485_A6L7_LEU1pro_F_H06_24_ABI24.ab1     1 CGATAAGGTG CAACAGTGTT GAGTTTCCCG AAACCAGGCA TTTTTGCGGT AGGGTCTGAA    61 ACTTTCACGA AACCATTTCA CAACAAAACT TCTTCAACAA CATGTGTGCA AAGAAAATCG   121 TCGTCTTACC AGGAGACCAT ATTGGCCCTG AAATTGTTGC TTCTGCCTTG GAGGTTTTGA   181 AAGTCGTTGA GAAGAAGCGA CCTGAGTTAA AACTCGAGTT TGAAGAACAC AAGATTGGAG   241 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG   301 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC   361 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC   421 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG   481 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA   541 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT   601 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACACCAAT GTTTATAACC AAGTTTTATC   661 TTGTTTGTCT ACATGGTATT TTACATTCAT CTACATACAT CTTTTCATTG GCTTTGTACA   721 TAGTTATCAT AACAAGTCTA AAGAATTCCT CCTTTTCTTA TTTCAATGTC AATCCCAAAA 781 GAAAAGAATT GTGGGTATTG TTGTAGGAGT CATGGTTAAA AGAAATCAGC ATG //
Primer: ars_R1 Sequence file: RDB14485_A6L7c.seq
>06186_14485_A6L7_ars_R1_E02_05_ABI08.ab1     1 TGGTTCGGTT TTATACTAAT TACTTAAGTA CCTTTAACTA AACAAAATGC CTATATATAT    61 ATTAATTTAC AATGAGTGTC AGATAAGTCA CTATGTCCGA GTGGTTAAGG AGTTAGACTC   121 GAATTCGCAA GCTTAGCTAC AAATCCCACT GGCTATATGT ATGCATTTGT GTTAAAAAAG   181 TTTGTATAGA TTATTTAATC TACTCAGCAT TCTTTCTCTA AATAGGAATT TGTTACTTAA   241 TGGAGAAAAA AATGTTTCGA TTTACCTAGT GTATTTGTTT GTATACTCAC GTTTAATTTC   301 AAACATCCAT TCTATCTTGT GTAATTTTTG GCATGGTGAA AAAGATAATC AGCCTTATAA   361 TCTTTACAAA AGTAAGAAAT TCTGTAAATA AGCCTTAATG CCCTTGCTTT AAATTAAAAT   421 GGTTCTTTTT CATGATAATG TTTGCACTTT GTGAATATAT TTTAGATAGT TCTGTGAGGT   481 ATAATTAAGA TGTTTTAGAG ACTTATACAA TTTTGTCTTT ATAAATTCTT AATTGATTTT   541 ACCATCCCAG TTTAACTATG CTTCGTCGGC ATCTCTGCAC ATGTCGTGTT TTCTTACCGT   601 ATTGTCCTAC CAAGAACCTC TTTTTTGCTT GGATCGAAAT TAAAGGTTTA AAAGCAAAGT   661 TATGGATGCT AGAGTATTTC AAAGCTATTC AGCTAGAGCT GAGGGGATGA AAAATCCCAT   721 TGCCAAGGAA TTGTTGGCTT TGATGGAAGA AAAGCAAAGC AACTTGTCAG TCGCGGTCGA   781 TTTGACGAAG AAATCCGAAA TCTTAGAATT GGTAGATAAA ATTGGACCCT ATGTCTGTGT   841 TATCAAGACA CATATTGACG TTGTCGAGGA TTTCGACCAG GATATGGTAG AAAAAACTGG   901 TGGCCCTTAG TAAAAAGCAT CGTTTTCTTA TCTTTGAGAT CGCAAATTCG CAGACATTGG   961 AAATACCGTC AGCTACAATA TGCATCTGGT GTGTACCAAA ATTGCTTTCC TTGGCTCATA  1021 ATCACAAATT GGCCATAACA GTGCAAGGCC GAAGGTATTA ATACAGGCTT CAAGAAGTGA 1081 ATTAACGTTG GGACCG //
Primer: f1ori_R3 Sequence file: RDB14485_A6L7d.seq
>06186_14485_A6L7_f1ori_R3_F02_06_ABI08.ab1     1 GCCATGCGCT TTGAAGTATG GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC    61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG   121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG   181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG   241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC   301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA   361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG   421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA   481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA   541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG   601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG   661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA   721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG   781 AGATGACATG CTATTTTCTG ATTATTTTTT TTCTATAGTT TTTCTATTTT TGTACGTCCT   841 TGGGAGTCCG AAATGTAAAA TCGGGGTATG GGTTAGTAGC GGCAGCGTTC ATTATGGTAT   901 ATAAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAAATGG GATACAAGGG CATCGTCTAA   961 TAATTATAGC TAAAAATTGT AATTTTAAAT TTGTATTTTT TTGTAAATTT TTATTTCTCA  1021 TGGTTTACTA AATGGAATGC GATTAGAGAA ACCTTCCACG AAATGTCAAT TAGCTCAGCC 1081 GAAGTATATA CGAA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl