Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-FFH1

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14484_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14484_A6L7a.seq
>06185_14484_A6L7_nmt1Fw_H01_08_ABI08.ab1     1 GAGAATTAAT TTAAATCAGG GGAAAAACGG TAACTCTCGG CTACTGGATG GTTCAGTCAC    61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC   121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA   181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT   241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC   301 CTGGATCCGG ATATCATGGA TTATAAGGAT GATGACGATA AACCCATGGA CTACAAGGAC   361 GACGATGACA AACATCACCA CCATCACCAC TGAGATCTCG ACTCTAGAGG ATCCGGCGCG   421 CCACTTCTAA ATAAGCGAAT TTCTTATGAT TTATGATTTT TATTATTAAA TAAGTTATAA   481 AAAAAATAAG TGTATACAAA TTTTAAAGTG ACTCTTAGGT TTTAAAACGA AAATTCTTAT   541 TCTTGAGTAA CTCTTTCCTG TAGGTCAGGT TGCTTTCTCA GGTATAGTAT GAGGTCGCTC   601 TTATTGACCA CACCTCTACC GGCAGATCCG CTAGGGATAA CAGGGTAATA TACTAGTAAG   661 ATGATTCTTT TATCGTCGAT AACGATGAGG ATGTAGACGA TTATGATGAG AGTGATTAGC   721 TTCTAAAATT TGGTGTTTTC AAACGCTATT TACAGTACAA TCTGAAAAAA TTGGATGAAC   781 ATTGTAAATG GTAGGATACG AGTTCCAATT AATGTATTTT TTTAAGTATT ACACTTAGCT   841 TATTAATTTT ATATATACAT TCTACCTCTG TGGGTTTAAA GACACTCGTA CACATACTCA   901 ATTATTTATG ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT TTTTGACATT   961 AAAAATTTCG TTAACTACGT AGAAGCGCTG ATATGAGCTA CTATTTTAAT TATCTAGAAA  1021 CAAGATCATT ATTATTAAGG TAAGCTTCAA GGATACTTGT AATTTCGTAA AGGCCAAGTC 1081 CAGGCCGAG //
Primer: LEU1pro_F Sequence file: RDB14484_A6L7b.seq
>06185_14484_A6L7_LEU1pro_F_A02_01_ABI08.ab1     1 AATAAAGGGT GCCAACAGTG TTGAGTTTCC CGAAACCAGG CATTTTTGCG GTAGGGTCTG    61 AAACTTTCAC GAAACCATTT CACAACAAAA CTTCTTCAAC AACATGTGTG CAAAGAAAAT   121 CGTCGTCTTA CCAGGAGACC ATATTGGCCC TGAAATTGTT GCTTCTGCCT TGGAGGTTTT   181 GAAAGTCGTT GAGAAGAAGC GACCTGAGTT AAAACTCGAG TTTGAAGAAC ACAAGATTGG   241 AGGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT   301 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG   361 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG   421 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT   481 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG   541 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT   601 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACACCA ATGTTTATAA CCAAGTTTTA   661 TCTTGTTTGT CTACATGGTA TTTTACATTC ATCTACATAC ATCTTTCATT GGCTTTGTAC   721 ATAGTTATCA TTACAAGTCT AAAAAAAATT CACTCTTTTC TTATTCAATG TCAATCCAAG   781 AGAAAAGATT GTGGGTAATG TTGTAGGAGC ATGTTTAATA AATTACTATA GCAAATTACT   841 TTTTTATTCC CAAGGTGTTT ATCTATAATA GTTAATATTT TTAGTCGCTA CATAAAATTT   901 TACCCAAAGA GTACTTGTAT ACTAAATTCT AAATGCCCTT CTGACATAAA ACGCCCTAGG   961 AAAACAAACG CAAACAAGGC ATCGACTTTT TCAATACCAA CGAAAAATTT TACATTAGTC  1021 CTTTTTTTTA ATGCCTGAGA AAGTCTTTGC TGGATATGCG TCGAACAGCT TCTCGATATT 1081 CTCTTGTCTT CGACAACCAG GGATTAACGA CTA //
Primer: ars_R1 Sequence file: RDB14484_A6L7c.seq
>06185_14484_A6L7_ars_R1_B02_02_ABI08.ab1     1 TGGTCCGGTT TTATACTAAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA    61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT   121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA   181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA   241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT   301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA   361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA   421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG   481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT   541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG   601 TATTGTCCTA CCAAGAACCC TCTTTTTTTG CTTGGATCGA AATTAAAGGT TTAAAAGCAA   661 AGTTATGGGA TGCTAGAGTA TTTCAAGCTA TTCAGCTAGA GCTGAGGATG AAAAATCCAT   721 TGCCAGGAAT TGTTGCTTGA TGAAGAAAAG CAAGCACTGG TCAGTCGCGT CGATTTGACG   781 AAGAATTCGG AAATCTAGAA TGTTAGAATA AATGGAACCT ATGGCTGGTA TCAGACCTAT   841 GACGTTGTCC AGGATTCGAT CAGAAATTGT GAACTAGTGC CTAGTAAAGC ACGTCTTATC 901 TTAGGATCGC //
Primer: f1ori_R3 Sequence file: RDB14484_A6L7d.seq
>06185_14484_A6L7_f1ori_R3_C02_03_ABI08.ab1     1 CGCTTAGGGT GCCTGAGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC    61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG   121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG   181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG   241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC   301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA   361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG   421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA   481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA   541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG   601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG   661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA   721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG   781 AGATGACATG CTATTTTTCT GATTATTTTT TTTTCTATAG TTTTTCTATT TTTGTACGTC   841 CCTTGGGGAG TCCGAAATGT AAAAATCGGG GGTATGGGTT AGTAGCGGGC AGCGTTTCAT   901 TATGGGTATA TAAAAAGATA AAAAAGTAAG GGGCAAGCGA AGACAGAAAT GGGAATACAA   961 AGGGCATCGT CTATAATTTA TAGCTAAAAA TTTGTATTTT AATTTGTATT TTTTTGTAAT  1021 TTTATTCTCA ATTGTTTACT AAATGGAATG CGAATTAGAG AAACTTCGAC GAACTGTCCG 1081 AGATTAGCCT TCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl