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pDUAL-FFH1

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06185
Resource name pDUAL-FFH1
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364052.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06185 pDUAL-FFH1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06185z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14484_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14484_A6L7a.seq check
            >06185_14484_A6L7_nmt1Fw_H01_08_ABI08.ab1
                1 GAGAATTAAT TTAAATCAGG GGAAAAACGG TAACTCTCGG CTACTGGATG GTTCAGTCAC
               61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC
              121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA
              181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT
              241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC
              301 CTGGATCCGG ATATCATGGA TTATAAGGAT GATGACGATA AACCCATGGA CTACAAGGAC
              361 GACGATGACA AACATCACCA CCATCACCAC TGAGATCTCG ACTCTAGAGG ATCCGGCGCG
              421 CCACTTCTAA ATAAGCGAAT TTCTTATGAT TTATGATTTT TATTATTAAA TAAGTTATAA
              481 AAAAAATAAG TGTATACAAA TTTTAAAGTG ACTCTTAGGT TTTAAAACGA AAATTCTTAT
              541 TCTTGAGTAA CTCTTTCCTG TAGGTCAGGT TGCTTTCTCA GGTATAGTAT GAGGTCGCTC
              601 TTATTGACCA CACCTCTACC GGCAGATCCG CTAGGGATAA CAGGGTAATA TACTAGTAAG
              661 ATGATTCTTT TATCGTCGAT AACGATGAGG ATGTAGACGA TTATGATGAG AGTGATTAGC
              721 TTCTAAAATT TGGTGTTTTC AAACGCTATT TACAGTACAA TCTGAAAAAA TTGGATGAAC
              781 ATTGTAAATG GTAGGATACG AGTTCCAATT AATGTATTTT TTTAAGTATT ACACTTAGCT
              841 TATTAATTTT ATATATACAT TCTACCTCTG TGGGTTTAAA GACACTCGTA CACATACTCA
              901 ATTATTTATG ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT TTTTGACATT
              961 AAAAATTTCG TTAACTACGT AGAAGCGCTG ATATGAGCTA CTATTTTAAT TATCTAGAAA
             1021 CAAGATCATT ATTATTAAGG TAAGCTTCAA GGATACTTGT AATTTCGTAA AGGCCAAGTC
            1081 CAGGCCGAG
            //
            Primer: LEU1pro_F
            Sequence file: RDB14484_A6L7b.seq check
            >06185_14484_A6L7_LEU1pro_F_A02_01_ABI08.ab1
                1 AATAAAGGGT GCCAACAGTG TTGAGTTTCC CGAAACCAGG CATTTTTGCG GTAGGGTCTG
               61 AAACTTTCAC GAAACCATTT CACAACAAAA CTTCTTCAAC AACATGTGTG CAAAGAAAAT
              121 CGTCGTCTTA CCAGGAGACC ATATTGGCCC TGAAATTGTT GCTTCTGCCT TGGAGGTTTT
              181 GAAAGTCGTT GAGAAGAAGC GACCTGAGTT AAAACTCGAG TTTGAAGAAC ACAAGATTGG
              241 AGGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT
              301 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG
              361 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG
              421 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT
              481 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG
              541 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT
              601 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACACCA ATGTTTATAA CCAAGTTTTA
              661 TCTTGTTTGT CTACATGGTA TTTTACATTC ATCTACATAC ATCTTTCATT GGCTTTGTAC
              721 ATAGTTATCA TTACAAGTCT AAAAAAAATT CACTCTTTTC TTATTCAATG TCAATCCAAG
              781 AGAAAAGATT GTGGGTAATG TTGTAGGAGC ATGTTTAATA AATTACTATA GCAAATTACT
              841 TTTTTATTCC CAAGGTGTTT ATCTATAATA GTTAATATTT TTAGTCGCTA CATAAAATTT
              901 TACCCAAAGA GTACTTGTAT ACTAAATTCT AAATGCCCTT CTGACATAAA ACGCCCTAGG
              961 AAAACAAACG CAAACAAGGC ATCGACTTTT TCAATACCAA CGAAAAATTT TACATTAGTC
             1021 CTTTTTTTTA ATGCCTGAGA AAGTCTTTGC TGGATATGCG TCGAACAGCT TCTCGATATT
            1081 CTCTTGTCTT CGACAACCAG GGATTAACGA CTA
            //
            Primer: ars_R1
            Sequence file: RDB14484_A6L7c.seq check
            >06185_14484_A6L7_ars_R1_B02_02_ABI08.ab1
                1 TGGTCCGGTT TTATACTAAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA
               61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT
              121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA
              181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA
              241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT
              301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA
              361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA
              421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG
              481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT
              541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG
              601 TATTGTCCTA CCAAGAACCC TCTTTTTTTG CTTGGATCGA AATTAAAGGT TTAAAAGCAA
              661 AGTTATGGGA TGCTAGAGTA TTTCAAGCTA TTCAGCTAGA GCTGAGGATG AAAAATCCAT
              721 TGCCAGGAAT TGTTGCTTGA TGAAGAAAAG CAAGCACTGG TCAGTCGCGT CGATTTGACG
              781 AAGAATTCGG AAATCTAGAA TGTTAGAATA AATGGAACCT ATGGCTGGTA TCAGACCTAT
              841 GACGTTGTCC AGGATTCGAT CAGAAATTGT GAACTAGTGC CTAGTAAAGC ACGTCTTATC
            901 TTAGGATCGC
            //
            Primer: f1ori_R3
            Sequence file: RDB14484_A6L7d.seq check
            >06185_14484_A6L7_f1ori_R3_C02_03_ABI08.ab1
                1 CGCTTAGGGT GCCTGAGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC
               61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG
              121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG
              181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG
              241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC
              301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA
              361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG
              421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA
              481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA
              541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG
              601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG
              661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA
              721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG
              781 AGATGACATG CTATTTTTCT GATTATTTTT TTTTCTATAG TTTTTCTATT TTTGTACGTC
              841 CCTTGGGGAG TCCGAAATGT AAAAATCGGG GGTATGGGTT AGTAGCGGGC AGCGTTTCAT
              901 TATGGGTATA TAAAAAGATA AAAAAGTAAG GGGCAAGCGA AGACAGAAAT GGGAATACAA
              961 AGGGCATCGT CTATAATTTA TAGCTAAAAA TTTGTATTTT AATTTGTATT TTTTTGTAAT
             1021 TTTATTCTCA ATTGTTTACT AAATGGAATG CGAATTAGAG AAACTTCGAC GAACTGTCCG
            1081 AGATTAGCCT TCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            user_report Takayama, S., Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe. Microb. Cell Fact. 17 (1): 176 (2018). PMID 30424766.
            user_report Dujeancourt, L., Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. Mitochondrion 13 (6): 871-880 (2013). PMID 23892058.
            user_report Kuhl, I., A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression. Nucleic Acids Res., 39 (18): 8029-8041 (2011). PMID 21727087.

            2022.05.18

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