RDB06185 - pDUAL-FFH1

Resource data sheet
Prev.

pDUAL-FFH1

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number	RDB06185
Resource name	pDUAL-FFH1
Clone info.	S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364052.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06185	pDUAL-FFH1	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06185z.seq from Depositor

Original reference

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

Further references such as user reports and related articles (go to bottom)

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14484_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw \| Sequence: RDB14484_A6L7a.seq
>06185_14484_A6L7_nmt1Fw_H01_08_ABI08.ab1 1 GAGAATTAAT TTAAATCAGG GGAAAAACGG TAACTCTCGG CTACTGGATG GTTCAGTCAC 61 CCAACGATTA CTGGGGAGAG AAAACAGGGC AAAAGCAAAG CTTAAAGGAA TCCGATTGTC 121 ATTCGGCAAT GTGCAGCGAA ACTAAAAACC GGATAATGGA CCTGTTAATC GAAACATTGA 181 AGATATATAA AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT 241 TTCAATCTCA TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC 301 CTGGATCCGG ATATCATGGA TTATAAGGAT GATGACGATA AACCCATGGA CTACAAGGAC 361 GACGATGACA AACATCACCA CCATCACCAC TGAGATCTCG ACTCTAGAGG ATCCGGCGCG 421 CCACTTCTAA ATAAGCGAAT TTCTTATGAT TTATGATTTT TATTATTAAA TAAGTTATAA 481 AAAAAATAAG TGTATACAAA TTTTAAAGTG ACTCTTAGGT TTTAAAACGA AAATTCTTAT 541 TCTTGAGTAA CTCTTTCCTG TAGGTCAGGT TGCTTTCTCA GGTATAGTAT GAGGTCGCTC 601 TTATTGACCA CACCTCTACC GGCAGATCCG CTAGGGATAA CAGGGTAATA TACTAGTAAG 661 ATGATTCTTT TATCGTCGAT AACGATGAGG ATGTAGACGA TTATGATGAG AGTGATTAGC 721 TTCTAAAATT TGGTGTTTTC AAACGCTATT TACAGTACAA TCTGAAAAAA TTGGATGAAC 781 ATTGTAAATG GTAGGATACG AGTTCCAATT AATGTATTTT TTTAAGTATT ACACTTAGCT 841 TATTAATTTT ATATATACAT TCTACCTCTG TGGGTTTAAA GACACTCGTA CACATACTCA 901 ATTATTTATG ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT TTTTGACATT 961 AAAAATTTCG TTAACTACGT AGAAGCGCTG ATATGAGCTA CTATTTTAAT TATCTAGAAA 1021 CAAGATCATT ATTATTAAGG TAAGCTTCAA GGATACTTGT AATTTCGTAA AGGCCAAGTC 1081 CAGGCCGAG //
Primer: LEU1pro_F \| Sequence: RDB14484_A6L7b.seq
>06185_14484_A6L7_LEU1pro_F_A02_01_ABI08.ab1 1 AATAAAGGGT GCCAACAGTG TTGAGTTTCC CGAAACCAGG CATTTTTGCG GTAGGGTCTG 61 AAACTTTCAC GAAACCATTT CACAACAAAA CTTCTTCAAC AACATGTGTG CAAAGAAAAT 121 CGTCGTCTTA CCAGGAGACC ATATTGGCCC TGAAATTGTT GCTTCTGCCT TGGAGGTTTT 181 GAAAGTCGTT GAGAAGAAGC GACCTGAGTT AAAACTCGAG TTTGAAGAAC ACAAGATTGG 241 AGGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT 301 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG 361 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG 421 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT 481 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG 541 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT 601 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACACCA ATGTTTATAA CCAAGTTTTA 661 TCTTGTTTGT CTACATGGTA TTTTACATTC ATCTACATAC ATCTTTCATT GGCTTTGTAC 721 ATAGTTATCA TTACAAGTCT AAAAAAAATT CACTCTTTTC TTATTCAATG TCAATCCAAG 781 AGAAAAGATT GTGGGTAATG TTGTAGGAGC ATGTTTAATA AATTACTATA GCAAATTACT 841 TTTTTATTCC CAAGGTGTTT ATCTATAATA GTTAATATTT TTAGTCGCTA CATAAAATTT 901 TACCCAAAGA GTACTTGTAT ACTAAATTCT AAATGCCCTT CTGACATAAA ACGCCCTAGG 961 AAAACAAACG CAAACAAGGC ATCGACTTTT TCAATACCAA CGAAAAATTT TACATTAGTC 1021 CTTTTTTTTA ATGCCTGAGA AAGTCTTTGC TGGATATGCG TCGAACAGCT TCTCGATATT 1081 CTCTTGTCTT CGACAACCAG GGATTAACGA CTA //
Primer: ars_R1 \| Sequence: RDB14484_A6L7c.seq
>06185_14484_A6L7_ars_R1_B02_02_ABI08.ab1 1 TGGTCCGGTT TTATACTAAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA 61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT 121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA 181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA 241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT 301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA 361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA 421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG 481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT 541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG 601 TATTGTCCTA CCAAGAACCC TCTTTTTTTG CTTGGATCGA AATTAAAGGT TTAAAAGCAA 661 AGTTATGGGA TGCTAGAGTA TTTCAAGCTA TTCAGCTAGA GCTGAGGATG AAAAATCCAT 721 TGCCAGGAAT TGTTGCTTGA TGAAGAAAAG CAAGCACTGG TCAGTCGCGT CGATTTGACG 781 AAGAATTCGG AAATCTAGAA TGTTAGAATA AATGGAACCT ATGGCTGGTA TCAGACCTAT 841 GACGTTGTCC AGGATTCGAT CAGAAATTGT GAACTAGTGC CTAGTAAAGC ACGTCTTATC 901 TTAGGATCGC //
Primer: f1ori_R3 \| Sequence: RDB14484_A6L7d.seq
>06185_14484_A6L7_f1ori_R3_C02_03_ABI08.ab1 1 CGCTTAGGGT GCCTGAGTAT GCGGTGTGAA TACCGCACAG ATGCGTAAGG AGAAAATACC 61 GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG 121 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG 181 TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTCGAGCTCG 241 GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT TTTCTTCTGA TTTTCCTACC 301 ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC CAGGAATGAC AGAAGCCTCA 361 TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA CACCGTTAAG TGTACGAGGG 421 CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT TTTTTAAGGT AAGGTGAGGA 481 TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA GACGAGAAGT TGCCAAAACA 541 TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG AAGTTTCAGC TAAGCGGCCG 601 CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC AGATGATTTA CAAGGTTTTG 661 CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT CAAGTTTACT AAAATTTACA 721 TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT TGCAAACGCG TTTTACAAAG 781 AGATGACATG CTATTTTTCT GATTATTTTT TTTTCTATAG TTTTTCTATT TTTGTACGTC 841 CCTTGGGGAG TCCGAAATGT AAAAATCGGG GGTATGGGTT AGTAGCGGGC AGCGTTTCAT 901 TATGGGTATA TAAAAAGATA AAAAAGTAAG GGGCAAGCGA AGACAGAAAT GGGAATACAA 961 AGGGCATCGT CTATAATTTA TAGCTAAAAA TTTGTATTTT AATTTGTATT TTTTTGTAAT 1021 TTTATTCTCA ATTGTTTACT AAATGGAATG CGAATTAGAG AAACTTCGAC GAACTGTCCG 1081 AGATTAGCCT TCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
user report	Takayama, S., Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe. Microb. Cell Fact. 17 (1): 176 (2018). PMID 30424766.
user report	Dujeancourt, L., Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. Mitochondrion 13 (6): 871-880 (2013). PMID 23892058.
user report	Kuhl, I., A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression. Nucleic Acids Res., 39 (18): 8029-8041 (2011). PMID 21727087.

2019.10.05

GNP_filter3_RDBDEP_html_190927.pl