RDB06184 - pDUAL-HFG81

Resource data sheet
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pDUAL-HFG81

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter

Catalog number	RDB06184
Resource name	pDUAL-HFG81
Clone info.	S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone	pDUAL (Plasmid)
Selectable markers	Amp^r
Reference of nucleotide sequence	AB364051.1 (DDBJ accession number)
Depositor	Yoshida, Minoru \| Matsuyama, Akihisa \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering Please visit Information of Request for Distribution.
Order form [Credit Card Payment] [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]

Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	【追加提供依頼書】GFP-A Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06184	pDUAL-HFG81	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB06184z.seq from Depositor

Featured content	Expression Vector Backbone of Schizosaccharomyces pombe (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14457_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw	RDB14457_A6L7a.seq
>06184_14457_A6L7_nmt1Fw_F08_06_ABI08.ab1 1 TGTTATTAAT TTAAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA 61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT 121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA 181 TGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA 241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCTA 301 TGCATCACCA CCATCATCAT ATGGATTATA AGGATGATGA CGATAAACCC ATGGGTAAAG 361 GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG 421 GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC 481 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTT 541 TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG CATGACTTTT 601 TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG 661 GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 721 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 781 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTA 841 ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC 901 AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC 961 AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCCT TCTTGAGTTT 1021 GTAACAGCTG CTGGGATTAC ACATGGGCAT GGATGAACTA TACAAAGATA TCATGAGATC 1081 TCGACTCTAG AGATCCGGCG CCGCCACTTC TAAATAAGCG AATTCTATGA TTTATGATTT 1141 TAATAATCAG TAGGTATAAA AAAAAATAGT TGTAATACAA GTTTTTAAAG GTTGACCCTC 1201 TTAAAGG //
Primer: ADH_rev	RDB14457_A6L7b.seq
>06184_14457_A6L7_ADH_rev_G05_20_ABI24.ab1 1 GGGGGAGTAA TCAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA 61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT 121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCT TTGTATAGTT 181 CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC 241 TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA 301 AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC 361 TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT 421 CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT 481 TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT 541 CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT 601 CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG 661 GGTATCTTGA AAAGCATTGA ACACCATAAC AGGAAAGTAG TGACAAGTGT TGGCCACGGA 721 ACAGGTAGTT TTCCAGTAGT GCAAATAAAT TTAAGGGTAA GTTTTCCGTA TGTTGCATCA 781 CCCTTCACCC TCTCCCACTG ACAGAAAAAT TTGTGCCCAT TAACATCACC AATCTAATCC 841 ACAAGAATTG GAACACCTCC AGGTGAAAAG TTCCTTCTTC CCTTTACCCA TGGGTTTATC 901 GTCCATCCAT CCCTTATTAA TCCCATGATG ATGGATGGGG GTG //
Primer: ADH-Forward	RDB14457_A6L7c.seq
>06184_14457_A6L7_ADH-Forward_G08_07_ABI08.ab1 1 TGCCCCCTTT TATTTGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT 61 ACTAGTAAGA TGATTCTTTT ATCGTCGATA ACGATGAGGA TGTAGACGAT TATGATGAGA 121 GTGATTAGCT TCTAAAATTT GGTGTTTTCA AACGCTATTT ACAGTACAAT CTGAAAAAAT 181 TGGATGAACA TTGTAAATGG TAGGATACGA GTTCCAATTA ATGTATTTTT TAAGTATTAC 241 ACTTAGCTTA TTAATTTTAT ATATACATTC TACCTCTGTG GGTTTAAAGA CAACTCGTAC 301 ACATACTCAA ATTATTTATG AACATTATAA TGCATATTCG TACTGTCTAT TAAACATTTT 361 TTTGACATTA AAAATTTCGT TTACTAACGT AGAAAGCGCT GATAATTGAG GCTACTATTT 421 TAATATCTAG AAACAAAGAT TCATTAATTA ATTAAGTAAG CTCCAGGATA CTTGTATATT 481 TCGTTAAAAA AGCGCAATTT CAACAATTCC TATGAACATC CATGATTTCA TCACAAAATC 541 AAACATATCA GGATTTAACG TGAATTATTT GCTTTCTTGT GATTTTCATC TGATTTCTGG 601 TCATTTACGT TACTGTAAAC AAGCTTTTTA AAAAAAGGAT TTTTTACGCA ATATTATCGA 661 TATCCCAATC TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT 721 TGCGGTAGGG TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG 781 TGTGCAAAGA AAATCGTCGT CTTACCAGGA GACCATATTG GCCCTGAAAT TGTTGCTTCT 841 GCCTTGGAGG TTTTGAAAGT CGTTGAGAAG AAGCGACCTG AGTTAAAACT CGAGTTTGAA 901 GAACACAAGA TTGGAGGTGC CTCTATTGAT GCCTATGGAA CCCCTTTGAC TGACGAGACT 961 GTGAAAGGCT TGTTTGGAAG CTGACGGTGT TCTTTTGGGT GCCGTTGGTG GTCCTGAATG 1021 GACCAACCCC AATTGTCGTC CTGAGCAAGG TTTATTGAAG CTTCGTAAGA GTATGGGTGT 1081 TTGGCACTTC GACTTGCACT TTGCCAGCAG TCTTTAGTCA GTACAGGCCA TTGAGCTGGA 1141 TCGGTGAGGT TCGATTGTGT GTACGAGAAC TTACCTTGAA GTGT //
Primer: ars_R1	RDB14457_A6L7d.seq
>06184_14457_A6L7_ars_R1_H08_08_ABI08.ab1 1 TGGGGTTCGG TTTTTTACTA ATTACTTAAG TACCTTTAAC TAAACAAAAT GCCTATATAT 61 ATATTAATTT ACAATGAGTG TCAGATAAGT CACTATGTCC GAGTGGTTAA GGAGTTAGAC 121 TCGAATTCGC AAGCTTAGCT ACAAATCCCA CTGGCTATAT GTATGCATTT GTGTTAAAAA 181 AGTTTGTATA GATTATTTAA TCTACTCAGC ATTCTTTCTC TAAATAGGAA TTTGTTACTT 241 AATGGAGAAA AAAATGTTTC GATTTACCTA GTGTATTTGT TTGTATACTC ACGTTTAATT 301 TCAAACATCC ATTCTATCTT GTGTAATTTT TGGCATGGTG AAAAAGATAA TCAGCCTTAT 361 AATCTTTACA AAAGTAAGAA ATTCTGTAAA TAAGCCTTAA TGCCCTTGCT TTAAATTAAA 421 ATGGTTCTTT TTCATGATAA TGTTTGCACT TTGTGAATAT ATTTTAGATA GTTCTGTGAG 481 GTATAATTAA GATGTTTTAG AGACTTATAC AATTTTGTCT TTATAAATTC TTAATTGATT 541 TTACCATCCC AGTTTAACTA TGCTTCGTCG GCATCTCTGC ACATGTCGTG TTTTCTTACC 601 GTATTGTCCT ACCAAGAACC TCTTTTTTGC TTGGATCGAA ATTAAAGGTT TAAAAGCAAA 661 GTTATGGATG CTAGAGTATT TCAAAGCTAT TCAGCTAGAG CTGAGGGGAT GAAAAATCCC 721 ATTGCCAAGG AATTGTTGGC TTTGATGGAA GAAAAGCAAA GCAACTTGTC AGTCGCGGTC 781 GATTTGACGA AGAAATCCGA AATCTTAGAA TTGGTAGATA AAATTGGACC CTATGTCTGT 841 GTTATCAAGA CACATATTGA CGTTGTCGAG GATTTCGACC AGGATATGGT AGAAAAACTG 901 GGTGGGCCTT AGGTAAAAAG CATCGTTTTC TTATCTTTGA GGATCGCAAA TTCGCAGACA 961 TTGGGAATAC CGTCAGCTAC ATATGCATCT GGTGTGTACA AATTGCTTCT TGGGCTCATA 1021 TCACAAATTG CCATACAGTG CCAGGCGGAG GGTATATACA GGCCTCAAGG AGGTTGGTTA 1081 CTTTTGGGAC GTGGTCCTCT TGCTTTTGCT GGAATGTCTT CAAGGGCTCA TGCTACTGGT 1141 TCTACCAGAG GAAAGCTAGA TGGGTTGGGG GAAGGCAATA CCGGAATATA TG //
Primer: f1ori_R3	RDB14457_A6L7e.seq
>06184_14457_A6L7_f1ori_R3_F01_16_ABI24.ab1 1 GCAGGGATGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC 121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA 181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG 241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC 301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC 361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT 421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA 481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT 541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG 601 GGCCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AAGTTTTGCA 661 TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA 721 CAAATGGGAT GATCTCTTCT CCATTGTTTC CTTTTCTTCT TGCAAACGCG TTTTACAAGA 781 GATGACATGC TATTTTCTGA ATATTTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTGGG 841 GAGTTCGAAT GTAAAATCGG GTATGGTAGT AGCGCAGCGT CATATGTATT AGGATTAAGT 901 AAGGCCAGCG AGAACGAATG GGATACTGGC ATCGTCTATA ATTATAGGCT AAAATGGAAT 961 TCGTGATTTT GCAATATGCC ATGTACTATG AATG //

Please visit Sequencing and PCR primers for primer information.

References

original	Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original	Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl