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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDUAL-HFG81

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06184
Resource name pDUAL-HFG81
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364051.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06184 pDUAL-HFG81 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06184z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14457_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14457_A6L7a.seq check
            >06184_14457_A6L7_nmt1Fw_F08_06_ABI08.ab1
                1 TGTTATTAAT TTAAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
               61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
              121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
              181 TGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA
              241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCTA
              301 TGCATCACCA CCATCATCAT ATGGATTATA AGGATGATGA CGATAAACCC ATGGGTAAAG
              361 GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG
              421 GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC
              481 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTT
              541 TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG CATGACTTTT
              601 TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG
              661 GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG
              721 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA
              781 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTA
              841 ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC
              901 AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC
              961 AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCCT TCTTGAGTTT
             1021 GTAACAGCTG CTGGGATTAC ACATGGGCAT GGATGAACTA TACAAAGATA TCATGAGATC
             1081 TCGACTCTAG AGATCCGGCG CCGCCACTTC TAAATAAGCG AATTCTATGA TTTATGATTT
             1141 TAATAATCAG TAGGTATAAA AAAAAATAGT TGTAATACAA GTTTTTAAAG GTTGACCCTC
            1201 TTAAAGG
            //
            Primer: ADH_rev
            Sequence file: RDB14457_A6L7b.seq check
            >06184_14457_A6L7_ADH_rev_G05_20_ABI24.ab1
                1 GGGGGAGTAA TCAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
               61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
              121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCT TTGTATAGTT
              181 CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC
              241 TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA
              301 AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC
              361 TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT
              421 CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT
              481 TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT
              541 CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT
              601 CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG
              661 GGTATCTTGA AAAGCATTGA ACACCATAAC AGGAAAGTAG TGACAAGTGT TGGCCACGGA
              721 ACAGGTAGTT TTCCAGTAGT GCAAATAAAT TTAAGGGTAA GTTTTCCGTA TGTTGCATCA
              781 CCCTTCACCC TCTCCCACTG ACAGAAAAAT TTGTGCCCAT TAACATCACC AATCTAATCC
              841 ACAAGAATTG GAACACCTCC AGGTGAAAAG TTCCTTCTTC CCTTTACCCA TGGGTTTATC
            901 GTCCATCCAT CCCTTATTAA TCCCATGATG ATGGATGGGG GTG
            //
            Primer: ADH-Forward
            Sequence file: RDB14457_A6L7c.seq check
            >06184_14457_A6L7_ADH-Forward_G08_07_ABI08.ab1
                1 TGCCCCCTTT TATTTGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
               61 ACTAGTAAGA TGATTCTTTT ATCGTCGATA ACGATGAGGA TGTAGACGAT TATGATGAGA
              121 GTGATTAGCT TCTAAAATTT GGTGTTTTCA AACGCTATTT ACAGTACAAT CTGAAAAAAT
              181 TGGATGAACA TTGTAAATGG TAGGATACGA GTTCCAATTA ATGTATTTTT TAAGTATTAC
              241 ACTTAGCTTA TTAATTTTAT ATATACATTC TACCTCTGTG GGTTTAAAGA CAACTCGTAC
              301 ACATACTCAA ATTATTTATG AACATTATAA TGCATATTCG TACTGTCTAT TAAACATTTT
              361 TTTGACATTA AAAATTTCGT TTACTAACGT AGAAAGCGCT GATAATTGAG GCTACTATTT
              421 TAATATCTAG AAACAAAGAT TCATTAATTA ATTAAGTAAG CTCCAGGATA CTTGTATATT
              481 TCGTTAAAAA AGCGCAATTT CAACAATTCC TATGAACATC CATGATTTCA TCACAAAATC
              541 AAACATATCA GGATTTAACG TGAATTATTT GCTTTCTTGT GATTTTCATC TGATTTCTGG
              601 TCATTTACGT TACTGTAAAC AAGCTTTTTA AAAAAAGGAT TTTTTACGCA ATATTATCGA
              661 TATCCCAATC TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT
              721 TGCGGTAGGG TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG
              781 TGTGCAAAGA AAATCGTCGT CTTACCAGGA GACCATATTG GCCCTGAAAT TGTTGCTTCT
              841 GCCTTGGAGG TTTTGAAAGT CGTTGAGAAG AAGCGACCTG AGTTAAAACT CGAGTTTGAA
              901 GAACACAAGA TTGGAGGTGC CTCTATTGAT GCCTATGGAA CCCCTTTGAC TGACGAGACT
              961 GTGAAAGGCT TGTTTGGAAG CTGACGGTGT TCTTTTGGGT GCCGTTGGTG GTCCTGAATG
             1021 GACCAACCCC AATTGTCGTC CTGAGCAAGG TTTATTGAAG CTTCGTAAGA GTATGGGTGT
             1081 TTGGCACTTC GACTTGCACT TTGCCAGCAG TCTTTAGTCA GTACAGGCCA TTGAGCTGGA
            1141 TCGGTGAGGT TCGATTGTGT GTACGAGAAC TTACCTTGAA GTGT
            //
            Primer: ars_R1
            Sequence file: RDB14457_A6L7d.seq check
            >06184_14457_A6L7_ars_R1_H08_08_ABI08.ab1
                1 TGGGGTTCGG TTTTTTACTA ATTACTTAAG TACCTTTAAC TAAACAAAAT GCCTATATAT
               61 ATATTAATTT ACAATGAGTG TCAGATAAGT CACTATGTCC GAGTGGTTAA GGAGTTAGAC
              121 TCGAATTCGC AAGCTTAGCT ACAAATCCCA CTGGCTATAT GTATGCATTT GTGTTAAAAA
              181 AGTTTGTATA GATTATTTAA TCTACTCAGC ATTCTTTCTC TAAATAGGAA TTTGTTACTT
              241 AATGGAGAAA AAAATGTTTC GATTTACCTA GTGTATTTGT TTGTATACTC ACGTTTAATT
              301 TCAAACATCC ATTCTATCTT GTGTAATTTT TGGCATGGTG AAAAAGATAA TCAGCCTTAT
              361 AATCTTTACA AAAGTAAGAA ATTCTGTAAA TAAGCCTTAA TGCCCTTGCT TTAAATTAAA
              421 ATGGTTCTTT TTCATGATAA TGTTTGCACT TTGTGAATAT ATTTTAGATA GTTCTGTGAG
              481 GTATAATTAA GATGTTTTAG AGACTTATAC AATTTTGTCT TTATAAATTC TTAATTGATT
              541 TTACCATCCC AGTTTAACTA TGCTTCGTCG GCATCTCTGC ACATGTCGTG TTTTCTTACC
              601 GTATTGTCCT ACCAAGAACC TCTTTTTTGC TTGGATCGAA ATTAAAGGTT TAAAAGCAAA
              661 GTTATGGATG CTAGAGTATT TCAAAGCTAT TCAGCTAGAG CTGAGGGGAT GAAAAATCCC
              721 ATTGCCAAGG AATTGTTGGC TTTGATGGAA GAAAAGCAAA GCAACTTGTC AGTCGCGGTC
              781 GATTTGACGA AGAAATCCGA AATCTTAGAA TTGGTAGATA AAATTGGACC CTATGTCTGT
              841 GTTATCAAGA CACATATTGA CGTTGTCGAG GATTTCGACC AGGATATGGT AGAAAAACTG
              901 GGTGGGCCTT AGGTAAAAAG CATCGTTTTC TTATCTTTGA GGATCGCAAA TTCGCAGACA
              961 TTGGGAATAC CGTCAGCTAC ATATGCATCT GGTGTGTACA AATTGCTTCT TGGGCTCATA
             1021 TCACAAATTG CCATACAGTG CCAGGCGGAG GGTATATACA GGCCTCAAGG AGGTTGGTTA
             1081 CTTTTGGGAC GTGGTCCTCT TGCTTTTGCT GGAATGTCTT CAAGGGCTCA TGCTACTGGT
            1141 TCTACCAGAG GAAAGCTAGA TGGGTTGGGG GAAGGCAATA CCGGAATATA TG
            //
            Primer: f1ori_R3
            Sequence file: RDB14457_A6L7e.seq check
            >06184_14457_A6L7_f1ori_R3_F01_16_ABI24.ab1
                1 GCAGGGATGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
              241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
              301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
              361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
              421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
              481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
              541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
              601 GGCCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AAGTTTTGCA
              661 TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA
              721 CAAATGGGAT GATCTCTTCT CCATTGTTTC CTTTTCTTCT TGCAAACGCG TTTTACAAGA
              781 GATGACATGC TATTTTCTGA ATATTTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTGGG
              841 GAGTTCGAAT GTAAAATCGG GTATGGTAGT AGCGCAGCGT CATATGTATT AGGATTAAGT
              901 AAGGCCAGCG AGAACGAATG GGATACTGGC ATCGTCTATA ATTATAGGCT AAAATGGAAT
            961 TCGTGATTTT GCAATATGCC ATGTACTATG AATG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl