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pDUAL-HFG81

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter

Catalog number RDB06184
Resource name pDUAL-HFG81
Clone info. S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter.
Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of nucleotide sequence AB364051.1 (DDBJ accession number)
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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RDB06184 pDUAL-HFG81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06184z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14457_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14457_A6L7a.seq
>06184_14457_A6L7_nmt1Fw_F08_06_ABI08.ab1
    1 TGTTATTAAT TTAAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA
  241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCTA
  301 TGCATCACCA CCATCATCAT ATGGATTATA AGGATGATGA CGATAAACCC ATGGGTAAAG
  361 GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG
  421 GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC
  481 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTT
  541 TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG CATGACTTTT
  601 TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG
  661 GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG
  721 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA
  781 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTA
  841 ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC
  901 AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC
  961 AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCCT TCTTGAGTTT
 1021 GTAACAGCTG CTGGGATTAC ACATGGGCAT GGATGAACTA TACAAAGATA TCATGAGATC
 1081 TCGACTCTAG AGATCCGGCG CCGCCACTTC TAAATAAGCG AATTCTATGA TTTATGATTT
 1141 TAATAATCAG TAGGTATAAA AAAAAATAGT TGTAATACAA GTTTTTAAAG GTTGACCCTC
1201 TTAAAGG
//
Primer: ADH_rev RDB14457_A6L7b.seq
>06184_14457_A6L7_ADH_rev_G05_20_ABI24.ab1
    1 GGGGGAGTAA TCAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA
   61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT
  121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCT TTGTATAGTT
  181 CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC
  241 TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA
  301 AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC
  361 TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT
  421 CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT
  481 TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT
  541 CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT
  601 CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG
  661 GGTATCTTGA AAAGCATTGA ACACCATAAC AGGAAAGTAG TGACAAGTGT TGGCCACGGA
  721 ACAGGTAGTT TTCCAGTAGT GCAAATAAAT TTAAGGGTAA GTTTTCCGTA TGTTGCATCA
  781 CCCTTCACCC TCTCCCACTG ACAGAAAAAT TTGTGCCCAT TAACATCACC AATCTAATCC
  841 ACAAGAATTG GAACACCTCC AGGTGAAAAG TTCCTTCTTC CCTTTACCCA TGGGTTTATC
901 GTCCATCCAT CCCTTATTAA TCCCATGATG ATGGATGGGG GTG
//
Primer: ADH-Forward RDB14457_A6L7c.seq
>06184_14457_A6L7_ADH-Forward_G08_07_ABI08.ab1
    1 TGCCCCCTTT TATTTGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
   61 ACTAGTAAGA TGATTCTTTT ATCGTCGATA ACGATGAGGA TGTAGACGAT TATGATGAGA
  121 GTGATTAGCT TCTAAAATTT GGTGTTTTCA AACGCTATTT ACAGTACAAT CTGAAAAAAT
  181 TGGATGAACA TTGTAAATGG TAGGATACGA GTTCCAATTA ATGTATTTTT TAAGTATTAC
  241 ACTTAGCTTA TTAATTTTAT ATATACATTC TACCTCTGTG GGTTTAAAGA CAACTCGTAC
  301 ACATACTCAA ATTATTTATG AACATTATAA TGCATATTCG TACTGTCTAT TAAACATTTT
  361 TTTGACATTA AAAATTTCGT TTACTAACGT AGAAAGCGCT GATAATTGAG GCTACTATTT
  421 TAATATCTAG AAACAAAGAT TCATTAATTA ATTAAGTAAG CTCCAGGATA CTTGTATATT
  481 TCGTTAAAAA AGCGCAATTT CAACAATTCC TATGAACATC CATGATTTCA TCACAAAATC
  541 AAACATATCA GGATTTAACG TGAATTATTT GCTTTCTTGT GATTTTCATC TGATTTCTGG
  601 TCATTTACGT TACTGTAAAC AAGCTTTTTA AAAAAAGGAT TTTTTACGCA ATATTATCGA
  661 TATCCCAATC TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT
  721 TGCGGTAGGG TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG
  781 TGTGCAAAGA AAATCGTCGT CTTACCAGGA GACCATATTG GCCCTGAAAT TGTTGCTTCT
  841 GCCTTGGAGG TTTTGAAAGT CGTTGAGAAG AAGCGACCTG AGTTAAAACT CGAGTTTGAA
  901 GAACACAAGA TTGGAGGTGC CTCTATTGAT GCCTATGGAA CCCCTTTGAC TGACGAGACT
  961 GTGAAAGGCT TGTTTGGAAG CTGACGGTGT TCTTTTGGGT GCCGTTGGTG GTCCTGAATG
 1021 GACCAACCCC AATTGTCGTC CTGAGCAAGG TTTATTGAAG CTTCGTAAGA GTATGGGTGT
 1081 TTGGCACTTC GACTTGCACT TTGCCAGCAG TCTTTAGTCA GTACAGGCCA TTGAGCTGGA
1141 TCGGTGAGGT TCGATTGTGT GTACGAGAAC TTACCTTGAA GTGT
//
Primer: ars_R1 RDB14457_A6L7d.seq
>06184_14457_A6L7_ars_R1_H08_08_ABI08.ab1
    1 TGGGGTTCGG TTTTTTACTA ATTACTTAAG TACCTTTAAC TAAACAAAAT GCCTATATAT
   61 ATATTAATTT ACAATGAGTG TCAGATAAGT CACTATGTCC GAGTGGTTAA GGAGTTAGAC
  121 TCGAATTCGC AAGCTTAGCT ACAAATCCCA CTGGCTATAT GTATGCATTT GTGTTAAAAA
  181 AGTTTGTATA GATTATTTAA TCTACTCAGC ATTCTTTCTC TAAATAGGAA TTTGTTACTT
  241 AATGGAGAAA AAAATGTTTC GATTTACCTA GTGTATTTGT TTGTATACTC ACGTTTAATT
  301 TCAAACATCC ATTCTATCTT GTGTAATTTT TGGCATGGTG AAAAAGATAA TCAGCCTTAT
  361 AATCTTTACA AAAGTAAGAA ATTCTGTAAA TAAGCCTTAA TGCCCTTGCT TTAAATTAAA
  421 ATGGTTCTTT TTCATGATAA TGTTTGCACT TTGTGAATAT ATTTTAGATA GTTCTGTGAG
  481 GTATAATTAA GATGTTTTAG AGACTTATAC AATTTTGTCT TTATAAATTC TTAATTGATT
  541 TTACCATCCC AGTTTAACTA TGCTTCGTCG GCATCTCTGC ACATGTCGTG TTTTCTTACC
  601 GTATTGTCCT ACCAAGAACC TCTTTTTTGC TTGGATCGAA ATTAAAGGTT TAAAAGCAAA
  661 GTTATGGATG CTAGAGTATT TCAAAGCTAT TCAGCTAGAG CTGAGGGGAT GAAAAATCCC
  721 ATTGCCAAGG AATTGTTGGC TTTGATGGAA GAAAAGCAAA GCAACTTGTC AGTCGCGGTC
  781 GATTTGACGA AGAAATCCGA AATCTTAGAA TTGGTAGATA AAATTGGACC CTATGTCTGT
  841 GTTATCAAGA CACATATTGA CGTTGTCGAG GATTTCGACC AGGATATGGT AGAAAAACTG
  901 GGTGGGCCTT AGGTAAAAAG CATCGTTTTC TTATCTTTGA GGATCGCAAA TTCGCAGACA
  961 TTGGGAATAC CGTCAGCTAC ATATGCATCT GGTGTGTACA AATTGCTTCT TGGGCTCATA
 1021 TCACAAATTG CCATACAGTG CCAGGCGGAG GGTATATACA GGCCTCAAGG AGGTTGGTTA
 1081 CTTTTGGGAC GTGGTCCTCT TGCTTTTGCT GGAATGTCTT CAAGGGCTCA TGCTACTGGT
1141 TCTACCAGAG GAAAGCTAGA TGGGTTGGGG GAAGGCAATA CCGGAATATA TG
//
Primer: f1ori_R3 RDB14457_A6L7e.seq
>06184_14457_A6L7_f1ori_R3_F01_16_ABI24.ab1
    1 GCAGGGATGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
  241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
  301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
  361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
  421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
  481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
  541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
  601 GGCCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AAGTTTTGCA
  661 TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA
  721 CAAATGGGAT GATCTCTTCT CCATTGTTTC CTTTTCTTCT TGCAAACGCG TTTTACAAGA
  781 GATGACATGC TATTTTCTGA ATATTTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTGGG
  841 GAGTTCGAAT GTAAAATCGG GTATGGTAGT AGCGCAGCGT CATATGTATT AGGATTAAGT
  901 AAGGCCAGCG AGAACGAATG GGATACTGGC ATCGTCTATA ATTATAGGCT AAAATGGAAT
961 TCGTGATTTT GCAATATGCC ATGTACTATG AATG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl