Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-HFG81

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (low strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14457_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14457_A6L7a.seq
>06184_14457_A6L7_nmt1Fw_F08_06_ABI08.ab1     1 TGTTATTAAT TTAAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA    61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT   121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA   181 TGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA   241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCTA   301 TGCATCACCA CCATCATCAT ATGGATTATA AGGATGATGA CGATAAACCC ATGGGTAAAG   361 GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG   421 GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC   481 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTT   541 TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG CATGACTTTT   601 TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG   661 GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG   721 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA   781 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTA   841 ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC   901 AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC   961 AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCCT TCTTGAGTTT  1021 GTAACAGCTG CTGGGATTAC ACATGGGCAT GGATGAACTA TACAAAGATA TCATGAGATC  1081 TCGACTCTAG AGATCCGGCG CCGCCACTTC TAAATAAGCG AATTCTATGA TTTATGATTT  1141 TAATAATCAG TAGGTATAAA AAAAAATAGT TGTAATACAA GTTTTTAAAG GTTGACCCTC 1201 TTAAAGG //
Primer: ADH_rev Sequence file: RDB14457_A6L7b.seq
>06184_14457_A6L7_ADH_rev_G05_20_ABI24.ab1     1 GGGGGAGTAA TCAGATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA    61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT   121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCT TTGTATAGTT   181 CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC   241 TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA   301 AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC   361 TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT   421 CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT   481 TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT   541 CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT   601 CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG   661 GGTATCTTGA AAAGCATTGA ACACCATAAC AGGAAAGTAG TGACAAGTGT TGGCCACGGA   721 ACAGGTAGTT TTCCAGTAGT GCAAATAAAT TTAAGGGTAA GTTTTCCGTA TGTTGCATCA   781 CCCTTCACCC TCTCCCACTG ACAGAAAAAT TTGTGCCCAT TAACATCACC AATCTAATCC   841 ACAAGAATTG GAACACCTCC AGGTGAAAAG TTCCTTCTTC CCTTTACCCA TGGGTTTATC 901 GTCCATCCAT CCCTTATTAA TCCCATGATG ATGGATGGGG GTG //
Primer: ADH-Forward Sequence file: RDB14457_A6L7c.seq
>06184_14457_A6L7_ADH-Forward_G08_07_ABI08.ab1     1 TGCCCCCTTT TATTTGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT    61 ACTAGTAAGA TGATTCTTTT ATCGTCGATA ACGATGAGGA TGTAGACGAT TATGATGAGA   121 GTGATTAGCT TCTAAAATTT GGTGTTTTCA AACGCTATTT ACAGTACAAT CTGAAAAAAT   181 TGGATGAACA TTGTAAATGG TAGGATACGA GTTCCAATTA ATGTATTTTT TAAGTATTAC   241 ACTTAGCTTA TTAATTTTAT ATATACATTC TACCTCTGTG GGTTTAAAGA CAACTCGTAC   301 ACATACTCAA ATTATTTATG AACATTATAA TGCATATTCG TACTGTCTAT TAAACATTTT   361 TTTGACATTA AAAATTTCGT TTACTAACGT AGAAAGCGCT GATAATTGAG GCTACTATTT   421 TAATATCTAG AAACAAAGAT TCATTAATTA ATTAAGTAAG CTCCAGGATA CTTGTATATT   481 TCGTTAAAAA AGCGCAATTT CAACAATTCC TATGAACATC CATGATTTCA TCACAAAATC   541 AAACATATCA GGATTTAACG TGAATTATTT GCTTTCTTGT GATTTTCATC TGATTTCTGG   601 TCATTTACGT TACTGTAAAC AAGCTTTTTA AAAAAAGGAT TTTTTACGCA ATATTATCGA   661 TATCCCAATC TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT   721 TGCGGTAGGG TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG   781 TGTGCAAAGA AAATCGTCGT CTTACCAGGA GACCATATTG GCCCTGAAAT TGTTGCTTCT   841 GCCTTGGAGG TTTTGAAAGT CGTTGAGAAG AAGCGACCTG AGTTAAAACT CGAGTTTGAA   901 GAACACAAGA TTGGAGGTGC CTCTATTGAT GCCTATGGAA CCCCTTTGAC TGACGAGACT   961 GTGAAAGGCT TGTTTGGAAG CTGACGGTGT TCTTTTGGGT GCCGTTGGTG GTCCTGAATG  1021 GACCAACCCC AATTGTCGTC CTGAGCAAGG TTTATTGAAG CTTCGTAAGA GTATGGGTGT  1081 TTGGCACTTC GACTTGCACT TTGCCAGCAG TCTTTAGTCA GTACAGGCCA TTGAGCTGGA 1141 TCGGTGAGGT TCGATTGTGT GTACGAGAAC TTACCTTGAA GTGT //
Primer: ars_R1 Sequence file: RDB14457_A6L7d.seq
>06184_14457_A6L7_ars_R1_H08_08_ABI08.ab1     1 TGGGGTTCGG TTTTTTACTA ATTACTTAAG TACCTTTAAC TAAACAAAAT GCCTATATAT    61 ATATTAATTT ACAATGAGTG TCAGATAAGT CACTATGTCC GAGTGGTTAA GGAGTTAGAC   121 TCGAATTCGC AAGCTTAGCT ACAAATCCCA CTGGCTATAT GTATGCATTT GTGTTAAAAA   181 AGTTTGTATA GATTATTTAA TCTACTCAGC ATTCTTTCTC TAAATAGGAA TTTGTTACTT   241 AATGGAGAAA AAAATGTTTC GATTTACCTA GTGTATTTGT TTGTATACTC ACGTTTAATT   301 TCAAACATCC ATTCTATCTT GTGTAATTTT TGGCATGGTG AAAAAGATAA TCAGCCTTAT   361 AATCTTTACA AAAGTAAGAA ATTCTGTAAA TAAGCCTTAA TGCCCTTGCT TTAAATTAAA   421 ATGGTTCTTT TTCATGATAA TGTTTGCACT TTGTGAATAT ATTTTAGATA GTTCTGTGAG   481 GTATAATTAA GATGTTTTAG AGACTTATAC AATTTTGTCT TTATAAATTC TTAATTGATT   541 TTACCATCCC AGTTTAACTA TGCTTCGTCG GCATCTCTGC ACATGTCGTG TTTTCTTACC   601 GTATTGTCCT ACCAAGAACC TCTTTTTTGC TTGGATCGAA ATTAAAGGTT TAAAAGCAAA   661 GTTATGGATG CTAGAGTATT TCAAAGCTAT TCAGCTAGAG CTGAGGGGAT GAAAAATCCC   721 ATTGCCAAGG AATTGTTGGC TTTGATGGAA GAAAAGCAAA GCAACTTGTC AGTCGCGGTC   781 GATTTGACGA AGAAATCCGA AATCTTAGAA TTGGTAGATA AAATTGGACC CTATGTCTGT   841 GTTATCAAGA CACATATTGA CGTTGTCGAG GATTTCGACC AGGATATGGT AGAAAAACTG   901 GGTGGGCCTT AGGTAAAAAG CATCGTTTTC TTATCTTTGA GGATCGCAAA TTCGCAGACA   961 TTGGGAATAC CGTCAGCTAC ATATGCATCT GGTGTGTACA AATTGCTTCT TGGGCTCATA  1021 TCACAAATTG CCATACAGTG CCAGGCGGAG GGTATATACA GGCCTCAAGG AGGTTGGTTA  1081 CTTTTGGGAC GTGGTCCTCT TGCTTTTGCT GGAATGTCTT CAAGGGCTCA TGCTACTGGT 1141 TCTACCAGAG GAAAGCTAGA TGGGTTGGGG GAAGGCAATA CCGGAATATA TG //
Primer: f1ori_R3 Sequence file: RDB14457_A6L7e.seq
>06184_14457_A6L7_f1ori_R3_F01_16_ABI24.ab1     1 GCAGGGATGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC    61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC   121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA   181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG   241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC   301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC   361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT   421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA   481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT   541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG   601 GGCCCAACCT TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AAGTTTTGCA   661 TAGAATCCCT AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA   721 CAAATGGGAT GATCTCTTCT CCATTGTTTC CTTTTCTTCT TGCAAACGCG TTTTACAAGA   781 GATGACATGC TATTTTCTGA ATATTTTTTT TCTATAGTTT TCTATTTTGT ACGTCCTGGG   841 GAGTTCGAAT GTAAAATCGG GTATGGTAGT AGCGCAGCGT CATATGTATT AGGATTAAGT   901 AAGGCCAGCG AGAACGAATG GGATACTGGC ATCGTCTATA ATTATAGGCT AAAATGGAAT 961 TCGTGATTTT GCAATATGCC ATGTACTATG AATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl